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Relationship Between Pod Characteristics of Some Cocoa Clones (T. cacaoL.) and Their Resistance Response to Cocoa Pod Borer Wahyu Susilo, Agung; Mangoendidjojo, Woerjono; ., Witjaksono
Pelita Perkebunan (Coffee and Cocoa Research Journal) Vol 23, No 3 (2007)
Publisher : Indonesian Coffee and Cocoa Research Institute

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Abstract

Characteristic study on CPB resistance had been carried out by characterizing mesocarp layer of cocoa pod where egg laying and larva penetration are carried out. For this study, 4 clones were selected which performed different response to CPB, namely resistant clones of KW 514 and ARDACIAR 10, moderately resistant clone of KW 411 and the susceptible clone of RCC 72. Pod samples were classified as young and mature were detached from cocoa collection at the experimental station of Kaliwining, Indonesian Coffee and Cocoa Research Institute in Jember. Characterization was conducted based on microchemical method at the Microtechnique Laboratory of Gadjah Mada University to identify trichome appearance at upper layer of pod, distributed granules of tannin through mesocarp and lignification of the sclerotic. It was characterized that the trichome density performing resistant in which the resistant and moderately resistant clones had more number of trichome density than of the susceptible one. The distributed granules of tannin at young pod showed significant difference among those clones that indicate resistant characteristics. The number of distributed granules of tannin was higher of those the resistant and moderatly resistant clones than of the susceptible clones that were formed both at furrow and at ridge of pod. However, the granules of tannin were distributed more at ridge than at furrow where furrow were preferred by CPB for egg laying. The characteristic differences between the resistant and moderately resistant clones were identified based on the intensity of lignification at sclerotic tissue. The lignified tissue of the resistant clones perform more intensive and more compact than of the moderately resistant clones. Tissue of young pod of the moderately resistant and susceptible clones was not yet lignified. Thickness of lignified tissue at the furrow of resistant clone was higher than of the moderately resistant and susceptible clones. Key words : Theobroma cocoa L.,cocoa pod borer, resistant, mesocarp, sclerotic, trichome, tannin.
Variasi Genetik Anggrek Alam Phalaenopsis amabilis (L.) Blume Hasil Iradiasi Sinar Gamma Sulistianingsih, Rahayu; Purwantoro, Aziz; Mangoendidjojo, Woerjono; Semiarti, Endang
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 8, No 1 (2012): Juni 2012
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1202.758 KB) | DOI: 10.17146/jair.2012.8.1.488

Abstract

Untuk mendapatkan kultivar baru anggrek alam Phalaenopsis amabilis (L.)Blume telah dilaksanakan iradiasi sinar gamma Co-60. Hasil iradiasi menunjukkan keragamanfenotip, sehingga perlu dilakukan penelitian secara biologi molekuler untuk mengetahuiapakah keragaman fenotip tersebut memang disebabkan karena adanya perbedaan genotippada tanaman-tanaman hasil iradiasi tersebut. Dalam penelitian ini dilakukan analisiskeragaman genetic antar individu dalam populasi tanaman hasil iradiasi dengan menggunakanteknik RAPD (Random Amplified Polymorphic DNA). Bahan tanaman berupa hasil iradiasi sinargamma 0, 15, 20, 25, 20+20 dan 40 Gray. Masing-masing diisolasi DNA genom dari tanamantersebut dan diamplifikasi dengan 22 primer yang ditentukan secara random. Hasil PCR(Polymease Chain Reaction) dianalisis dengan elektrophoresis dengan 1.5 % agarose. AnalisisDNA dilakukan dengan teknik RAPD menggunakan 8 primer terseleksi dari 22 primer yangdigunakan analisis polimorfis dan keragaman molekuler dianalisis dengan metode Nei’s genediversity dengan program GenAlEx 6.1. Hasil penelitian menunjukkan Keragaman geneticdapat dianalisis pada awal pertumbuhan tanaman anggrek bulan alam Phalaenopsis amabilis(L.) Blume hasil iradiasi sinar gamma Co-60 dengan menggunakan teknik RAPD dan dosisiradiasi 15 dan 40 Gy memberikan variabilitas genetik tinggi dibandingkan tanpa perlakuan
Analisis Interaksi Genotip X Lingkungan Tanaman Perkebunan (Studi Kasus pada Tanaman Teh) Mangoendidjojo, Woerjono
Zuriat Vol 11, No 1 (2000)
Publisher : Zuriat

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Abstract

Interaksi genotip dengan lingkungannya (G× E) merupakan hal yang sangat penting untuk diketahui oleh para pemulia tanaman, karena keragaan suatu genotip yang ditanam pada berbagai lingkungan dapat berbeda. Keberadaan interaksi (G × E) dapat menimbulkan kesulitan dalam program pemuliaan, meskipun demikian analisis (G × E) banyak digunakan untuk memperkirakan seberapa jauh daya adaptasi dan stabilitas yang dimiliki oleh suatu varietas. Lingkungan tumbuh tanaman, khususnya tanaman perkebunan untuk analisis (G × E) dapat bersifat spatial dan sequential. Analisis (G × E) yang dilakukan ini merupakan studi kasus pada tanaman teh yang ditanam di kebun Pagilaran menggunakan data hasil sepuluh klon yang diuji selama satu tahun dengan bulan sebagai lingkungan yang bersifat spatial. Hasil analisis menunjukkan bahwa Sembilan dari sepuluh klon yang dievaluasi, mempunyai stabilitas yang baik padaberbagai lingkungan. Tiga klon yang menduduki peringkat teratas berturutturut adalah GPPS-1/2678, MPS-7/2109/2, dan MPS-5/140/1. Klon TRI-2025 yang merupakan klon unggulan selama ini hanya menduduki peringkat ke tujuh.
Pendugaan Kemajuan Seleksi Gabungan Keturunan Saudara Tiri dan S1 pada Populasi Jagung Bisma Murti, Rudi Hari; Nasrullah, ,; Mangoendidjojo, Woerjono
Zuriat Vol 11, No 1 (2000)
Publisher : Zuriat

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Abstract

Tujuan penelitian adalah mendapatkan pembobot yang unique untuk seleksi gabungan dan mendapatkan nilai duga kemajuan genetik seleksi gabungan keturunan saudara tiri dan keturunan menyerbuk sendiri. Percobaan dilakukan di Kebun Percobaan, Penelitian dan Pengembangan Pertanian Kalitirtro (KP4), Universitas Gadjah Mada, Yogyakarta mulai Juli sampai Oktober 1999. Sebanyak 81 pasang keturunan, masing-masing pasang terdiri satu keturunan menyerbuk sendiri dan empat keturunan saudara tiri dievaluasi dengan menggunakan petak baris tunggal (delapan tanaman per baris). Rancangan yang digunakan adalah rancangan acak kelompok lengkap dengan tiga ulangan. Hasil penelitian menunjukkan komponen vektor penciri yang digunakan sebagai pembobot keturunan menyerbuk sendiri jauh lebih besar (0.93-0.99) daripada pembobot keturunan saudara tiri (0.07-0.34) untuk semua variabel yang diamati. Kemajuan seleksi harapan paling besar terjadi pada berat kering biji per tongkol (10.23% per siklus) dan paling kecil diameter tongkol (1.31%). Kemajuan seleksi gabungan yang lebih besar daripada seleksi individualnya hanya terjadi pada panjang tongkol yaitu 4.11% dan terjadi sebaliknya pada variabel yang lain.
Tanggapan Planlet Vanili yang Diradiasi dengan Sinar Gamma dan Keragaannya Setelah Diinokulasi dengan Jamur Fusarium oxysporum f.sp. vanillae Mangoendidjojo, Woerjono; Hadisutrisno, Bambang; Inayati, Alfi
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 2 (2000)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12390

Abstract

Vanilla is one of spice crops and important to world commerce. Indonesia is one of producing and exporting countries for decades. Recently, the planted area tends to decrease due to several factors. One of them is fusarium wilt caused by Fusarium oxysporum fsp. vanillae. The disease ranked among the most devastating disease attacking vanilla plants. The most effective method controlling fusarium wilt is the use of resistant varieties. The study aimed to know responses of vanilla plantlets irradiated by Gamma rays at several doses (0-4 krad). Five months after irradiation, the survival mutant plantlets were inoculated with suspension of the F. oxysporum fsp. vanillae to evaluate their resistance. Data of first concern taken were survival plantlets; number of leave, root, and sucker initiation; and infection intensity. The results indicated that higher the doses, decreased the survival plantlets. The applied doses performed significantly differences on the number of leave, root, and suckerinitiation. The untreated plantlets showed better responses as compared to those treated. Evaluation on their resistance after inoculation indicated that the survival plantlets irradiated at 3 and 1 krad showed lower infection intensity, eventhough variation within a treatment was observed. Evaluation on the individual plantlet basis seemed to be more helpful in order to identify mutant plantlets with better resistance.
Kandungan Katekin dan Kualitas (Warna Air Seduhan, Flavor, Kenampakan) Enam Klon Teh (Camellia sinensis (L.) O. Kuntze) di Ketinggian yang Berbeda Mitrowihardjo, Suyadi; Mangoendidjojo, Woerjono; Hartiko, Hari; Yudono, Prapto
Agritech Vol 32, No 2 (2012)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.9632

Abstract

The purpose of the study was to fi nd out high catechins content and quality of six tea clones which might contribute to tea clone improvements in the future. Altitudes growing (1200 – 1300 m above sea level and 700 – 900 m above sea level) which might infl uence the quality were also evaluated. Catechin (C), epicatechin (EC), epigallocatechin (EGC), apicatechin gallate (ECG), epigallocatechin gallate (EGCG) were analysed using high performance liquid chromatography (HPLC) method and tea quality (color, fl avor, appearance) evaluation were performed by three certifi ed tea testers. The results indicated that at higher altitude TRI 2025, PGL 10, and GMB 9 clones showed high total catechins, while at lower altitude PGL 15, GMB 9, and PGL 10 clones showed such high total catechins. Liquor colour scores were not signifi cantly different either between clones or locations, but liquor fl avor scores were found higher on GMB 7 and PGL 15 at either higher or lower altitudes than the rest of the clones evaluated. Liquor flavor scores tend to be better at lower altitude than those at higher altitude. At higher altitude, PGL 15, TRI 2025, GMB 9 performed highest infusion appearance scores, while at lower altitude, TRI 2025 clone showed the highest score. Similar to flavor scores, there was a tendency that the infusion appearance scores were much better at lower altitude than those at higher altitude.ABSTRAKPenelitian bertujuan untuk mendapatkan informasi klon-klon yang dapat memberikan katekin dan kualitas hasil yang tinggi yang diharapkan bermanfaat sebagai arahan perbaikan dalam pengembangan tanaman teh ke depan. Ketinggian tempat tumbuh (1200 – 1300 m dari permukaan laut dan 700 – 900 m dari permukaan laut) yang diduga berpengaruh terhadap kualitas hasil juga dievaluasi, karena terkait dengan ketersediaan lahan pengembangan. Analisis catechin (C), epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG), epigallocatechin gallate (EGCG) dilakukan dengan metode HPLC (High Performance Liquid Chromatography), sedang evaluasi kualitas (warna air seduhan, flavor, kenampakan) dilakukan oleh tiga orang tester teh bersertifi kat. Hasil analisis menunjukkan bahwa total katekin yang tinggi diperlihatkan TRI 2025, PGL 10, GMB 9 di lokasi ketinggian 1200-1300 m dpl, dan total katekin tinggi juga diperlihatkan PGL 15, GMB 9, dan PGL 10 di lokasi ketinggian 700 – 900 m dpl. Skor warna air seduhan tidak menunjukkan perbedaan antar klon maupun antar lokasi, namun skor flavor GMB 7 dan PGL 15 unggul di lokasi atas maupun di lokasi bawah. Ada kecenderungan skor fl avor lebih unggul di lokasi bawah dibanding dengan lokasi atas. Skor kenampakan ampas setelah teh diseduh tinggi untuk PGL 15, TRI 2025, dan GMB 9 di lokasi atas, sedang TRI 2025 tinggi di lokasi bawah. Serupa dengan skor fl avor, skor kenampakan ampas teh berkecenderungan unggul di lokasi bawah.