W. Manalu
Faculty of Veterinary Medicine, Bogor Agricultural University, Jl. Agatis, Darmaga Campus, Bogor 16680

Published : 22 Documents
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Relationship Between Milk Potassium Ion Concentration and Bacterial Cell Count, Somatic Cell Count and California Mastitis Test Score in Lactating Ewes

Media Veteriner Vol 7, No 1 (2006): Media Veteriner
Publisher : Media Veteriner

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Abstract

Thirty two lactating ewes were observed for 12 weeks to study the relationship between the concentration of milk potassium ion and the total bacterial count, somatic cell count and the scores of Californian mastitis test (CMT)in an effort to design an alternative method of detecting subdinical mastitis. Milk samples were collected weekly in the morning and tested for 12 weeks during lactation period. Parameters used in the study were the concentration of milk potassium ion, total bacterial cellcount, somatic cell count, and CMT scores. The result showed that the increase in bacterial cell count and somatic cell count was closely related with the increase in the concentration of milk potassium ion (

Progesterone and Estradiol Profil In Ewes Injected With Prostaglandin (PGF,,) in Late Pregnancy

Media Veteriner Vol 3, No 2 (1996): Media Veteriner
Publisher : Media Veteriner

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Abstract

Suatu percobaan telah dilakukan untuk mempelajari profil progesteron dan estradiol pada domba bunting yang korpus luteumnya telah dihilangkan pada akhir kebuntingan.Percobaan menggunakan tiga puluh lima domba yang terdiri dari dua puluh delapan domba bunting (14 ekor disuntik dengan prostaglandin F2, sintetik sedangkan 14 ekor lainnya tidak menerima penyuntikan prostaglandin F2=), dan tujuh ekor domba yang tidak bunting dan bersiklus sebagai kontrol. Domba percobaan dipelihara dalam kandang dengan makananrumput raja kering ditambah konsentrat. vitamin dan mineral Air minum tersedia secara ad libitum. Setiap minggu sampel darah diambil dari vena jugularis dengan menggunakanvacutainer steril untuk mendapatkan serum yang selanjutnya akan digunakan untuk menganalisa progesteron dan estradiol dengan radioimmunoassay fase padat Hasil percobaanmenunjukan bahwa penyuntikan prostaglandin pada akhir kebuntingan pada dornba tidak menyebabkan penurunan konsentrasi progesteron dan estradiol yang bermakna dalam serum induk. Luteolisis oleh prostaglandin F:,. tidak bermakna menurunkan konsentrasi progesteron dan estradiol dalam serum induk. Disimpulkan bahwa pada akhir kebuntinganpada domba, sumbangan korpus luteum terhadap total konsentrasi progesteron dan estradiol serta perkembangan kelenjar susu induk sangat kecil.

Feeding Broodstock on a Diet Containing Vitamin E and Fish Oil Improve Eggs and Larval Quality of Nile tilapia (Oreochromis niloticus)

Jurnal Akuakultur Indonesia Vol 7, No 1 (2008): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

The experiment was conducted to determine the effect of dietary vitamin E and n-3 fatty acids on the gonad maturation, egg and larva quality of Nile tilapia. Fish were treated by various combinations of dietary dosage of vitamin E (50, 100, 150, and 200 mg/kg feed) and fish oil (10, 20, 30, and 40 g/kg feed). Three hundreds and twenty pairs of broodstock fish (female at stage of maturity II) were selected and used for this experiment. Fish were fed on the experimental diets three times a day at satiation. The tested of parameters such as gonad somatic index, egg diameter, fecundity, number of spawned fish, hatching rate, and survival rate. Results of the experiment indicated that supplementation of vitamin E and fish oil stimulated gonad development and increased fecundity, hatching rate and survival rate of Nile tilapia larva. Combination of vitamin E 150 mg/kg feed and fish oil 30 g/kg feed significantly improved egg and larva quality of Nile tilapia. Thus, combination of vitamin E in 150 mg/kg diet with fish oil in dose 30 g/kg diet give the best reproduction performance. Keywords: Nile tilapia,  fish oil, vitamin E, reproduction performance   ABSTRAK Penelitian ini dilakukan untuk mendapatkan kadar kombinasi vitamin E dan asam lemak esensial n-3 untuk pematangan gonad, kualitas telur dan larva ikan nila. Adapun dosis dari masing-masing perlakuan terdiri dari 4 taraf. Untuk vitamin E yaitu: 50 mg/kg, 100 mg/kg, 150 mg/kg,  dan 200 mg/kg pakan  sedangkan minyak ikan yaitu: 10 g/kg, 20 g/kg, 30 g/kg dan 40 g/kg pakan. Ikan uji yang digunakan sebanyak 320 ekor induk betina dan 320 ekor induk jantan dan diseleksi.  Setiap hari ikan diberi pakan uji 2 kali sehari (pagi dan sore) secara at satiation. Selama periode pemeliharaan parameter yang diamati meliputi: indeks gonad somatik, diameter telur, fekunditas, jumlah induk yang memijah, derajat tetas telur, dan ketahanan hidup larva. Hasil penelitian ini menunjukkan bahwa pakan dengan kombinasi dosis vitamin E 150 mg/kg dan minyak ikan 30 g/kg memberi respons terhadap perkembangan gonad, fekunditas, derajat tetas telur dan ketahanan hidup larva ikan nila.  Kombinasi vitamin E 150 mg/kg dan minyak ikan 30 g/kg pakan memberi pengaruh signifikan dalam meningkatkan kualitas telur dan larva ikan nila. Dengan demikian, kombinasi vitamin E 150 mg/kg dan minyak ikan 30 g/kg pakan adalah memberi hasil terbaik pada performa reproduksi. Kata kunci: ikan nila, minyak ikan, vitamin E, performa reproduksi

Ovarian development of female mud crab, Scylla serrata supplemented with cholesterol and injected with serotonin

Jurnal Akuakultur Indonesia Vol 9, No 1 (2010): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

Cholesterol is known to play an important role in nutrition of crustacean and function as a precursor for steroids synthesis, while neurohormone of serotonin could induce ovarian maturation in crustacean. Ovarian development of adult females Scylla serrata was induced by adding cholesterol in the diet and serotonin injection. This research was designed to study the effectiveness of cholesterol supplementation and serotonin injection in ovarian development. Broodstocks were stocked in nine experimental units in three fiber tanks. The fiber tank was equipped with sands substrate and flow through seawater system. The experimental crabs were assigned into a completely randomized design with a 3 x 3 factorial arrangement. The first factor was cholesterol supplementation in the diet with 3 levels (0, 0,5 and 1,0%). The second factor was serotonin injection with 3 levels (0, 5 and 10 μg/g BW). Samples of broodstock were taken every four days to evaluate the stages of ovarian maturity and parameters were used to evaluate the ovarian maturation stage are gonad index (GI) and oocyte diameter, concentration of estradiol 17β, yolk protein concentrations, and fecundity. Results showed that female crabs supplemented with 0,5% cholesterol and a combination of cholesterol 0,5% supplementation and injection serotonin with a dose of 10 μg/g BW had better reproduction development. It is concluded that ovarian development of Scylla serrata could be improved by cholesterol supplementation and serotonin injection. Key words: Cholesterol, serotonin, ovarian development, Scylla serrata   ABSTRAK Kolesterol diketahui merupakan nutrien spesifik yang berperan dalam sisntesis hormon steroid dan mengontrol reproduksi, sementara serotonin merupakan salah satu neurohormon yang dilaporkan dapat merangsang pematangan ovari dan pemijahan pada krustase. Penelitian ini bertujuan untuk mengetahui pengaruh tingkat pemberian kolesterol yang optimal dalam pakan buatan, serta dosis penyuntikan serotonin yang efektif untuk mempercepat proses perkembangan dan pematangan ovarium induk kepiting bakau Scylla serrata. Pemeliharaan induk dilakukan dengan menggunakan tiga buah bak fiber. Bak pemeliharaan dilengkapi dengan substrat pasir dan sistim air laut mengalir. Eksperimen menggunakan rancangan acak lengkap (RAL) pola faktorial, dengan 9 satuan percobaan. Faktor pertama, suplemen kolesterol didalam pakan dengan 3 tingkat dosis (0; 0,5; dan 1%) dan faktor kedua, injeksi serotonin dengan 3 tingkat dosis (0, 5, dan 10 μg/g bobot tubuh). Pengamatan terhadap tingkat kematangan ovari dilakukan setiap 4 hari sekali. Paramater pengambilan sampel meliputi  tingkat kematangan ovari, indeks gonad dan diameter oosit, konsentrasi estradiol 17β, konsentrasi protein yolk, dan fekunditas. Hasil penelitian menunjukkan bahwa induk kepiting yang disuplementasi dengan dosis kolesterol 0,5% dan induk kepiting yang mendapat perlakuan kombinasi, suplementasi kolesterol 0,5% dan injeksi serotonin dosis 10 μg/g bobot tubuh dapat menghasilkan perkembangan ovari yang terbaik. Jadi kolesterol dan serotonin dapat digunakan untuk meningkatkan perkembangan ovari. Kata-kata kunci: Kolesterol, serotonin, perkembangan ovari, Scylla serrata

Gumboro Disease: Etiology, Epidemiology, Pathology, Diagnosis And Disease Control

WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 21, No 3 (2011): SEPTEMBER 2011
Publisher : Indonesian Center for Animal Research and Development

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Abstract

Infectious bursal disease (IBD) or known as Gumboro, is a disease that attacks chicken older than 3 weeks, caused by famili Birnaviridae virus. Gumboro in Indonesia was firstly reported in 1983 and until now is commonly found. Very virulent IBD virus causes high morbidity and mortality that can even reach 100%. Clinical symptoms are exhibited as sluggish chicken, dropped wings and cloacal pasting. At gross examination, the bursa was found swollen, with yellowish fluid or hemorrhagic 3 days after infection. The bursa will get atrophy from 7 days post-infection. Meanwhile, the non virulent IBD virus causes subclinical symptoms. Chicken that survived, became stunted or dwarfed. On gross and histopathological findings, the bursa Fabricius has mild lesion and will recover at 14 days post-infection. Diagnosis of IBD can be determined based on pathological observation, supported by immunohistochemical examination and laboratory confirmation of disease by agar gel immunodiffusion, polymerase chain reaction techniques, antigen capture enzyme linked immunosorbent assay and isolation. Detection of antibodies can be made by serum neutralization technique or enzyme linked immunosorbent assay. Prevention can be done by routine vaccination in the field when the maternal antibodies have declined. The review describes the etiology, epidemiology, pathogenesis clinical symptoms, pathological discription and control of the disease to improve the knowledge of poultry farmer or people who are interested in poultry health. Key words: Gumboro, etiology, epidemiology, pathology, diagnosis

PENGARUH PENAMBAHAN MINERAL Fe PADA PAKAN IKAN TERHADAP STATUS KESEHATAN IKAN KERAPU BEBEK (Cromileptes aftivelis)

Jurnal Perikanan Dan kelautan Vol 12, No 01 (2007)
Publisher : Jurnal Perikanan Dan kelautan

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Abstract

This study was conducted to find out the health status of humpback grouper fish((´hrtmtileptes altiverts) that are fed on inorganic-iron supplement. Both, thetreatment and the control was tested in anoxia condition. The fish (average weight4.18±0.13 g/ind) was cultured in 60x40x50 cm3 aquarium and stocked by 10indJaquarium. The fish was reared for 10 weeks: and they were fed threetimes/day at satiation. Results shown that the fish fed by non-iron supplement wasmore susceptible than the normal condition; base on the index of leucocytes,nionocytes. phagocytic and MCHC distribution. However, 25 mg Fe/kgsupplement in the diet was increased concentration of Fe by 35% - 100% in liver,intestine, body tissue and enhance fish vitality at anoxia condition. The survivalrate of fish increased by 1.3 times compare to the non-iron supplementation.

Maternal Serum Concentrations of Several Hormones in Does Bearing Different Fetal Number

Buletin Peternakan 1995: BULETIN PETERNAKAN SPECIAL EDITION 1995
Publisher : Fakultas Peternakan UGM

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The Effect of Corpora Luteal Number on Serum Progesterone and Estradiol of Ewes During Luteal Phase of Estrous Cycle and Pregnancy

Buletin Peternakan 1995: BULETIN PETERNAKAN SPECIAL EDITION 1995
Publisher : Fakultas Peternakan UGM

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Contributions of Maternal Serum Progesterone and Estradiol Concentrations or Corpora Luteal and Fetal Number to Mammary Growth and Development of Ewes During Pregnancy

Buletin Peternakan 1995: BULETIN PETERNAKAN SPECIAL EDITION 1995
Publisher : Fakultas Peternakan UGM

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The Effect of Fetal Number on Maternal Serum Progesterone and Estradiol of Ewes During Pregnancy

Buletin Peternakan 1995: BULETIN PETERNAKAN SPECIAL EDITION 1995
Publisher : Fakultas Peternakan UGM

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