Articles

Found 37 Documents
Search

Diurnal Patterns of Testosterone and Cortisol Metabolites in Fecal of Javan Gibbons (Hylobates moloch) in Captivity

HAYATI Journal of Biosciences Vol 13, No 2 (2006): June 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (78.12 KB)

Abstract

The aims of this research were to determine diurnal patterns of testosterone and cortisol metabolites to predict the testis functional status. In this study, fecal testosterone and cortisol were quantified in 77 samples from three male Hylobates moloch during a course of three months period. These data showed that the highest concentration of fecal testosterone occured at 18.00-06.00 (23.61 ng/g dried feces), then declined gradually. The lowest concentration was in the evening (5.54 ng/g dried feces). Our tests showed that there was a decrease in the mean testosterone concentration from 06.00-10.00 to 10.00-14.00 to 14.00-18.00. For cortisol, the highest concentration occured at 06.00-10.00 (597.84 ng/g dried feces), then decline gradually in the evening (225.73 ng/g dried feces). Key words: Hylobates moloch, feces, testosterone, cortisol, diurnal pattern

Quality of Epididymal and Ejaculated Sperms of Spotted Buffalo in Dextrose Supplemented Extender

HAYATI Journal of Biosciences Vol 17, No 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (32.619 KB)

Abstract

Quality of epididymal and ejaculated sperms of spotted buffalo in three different extenders (i.e. Andromed, Andromed supplemented with 0.2% dextrose, and Andromed supplemented with 0.4% dextrose) was studied.  The results showed that there was no significantly different (P > 0.05) quality of ejaculated and epididymal sperms.  The motility percentage of post thawing epididymal and ejaculated sperms was 41-46% and 41-45%, respectively.  And percentage of cytoplasmic membrane integrity of the post thawing epididymal and ejaculated sperms was 66-67% and 47-54% respectively. There was also no significant different (P > 0.05) in the percentage of cytoplasmic membrane integrity between the extenders of both ejaculated and epididymal sperms. This result suggested that epididymal sperms were reliable for artificial insemination as good as ejaculated sperms.

Fecal Steroid Profile of Female Javan Gibbons (Hylobates moloch) Maintained in Pairing-Typed Cage

HAYATI Journal of Biosciences Vol 17, No 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (528.43 KB)

Abstract

Estrone conjugate (E1C) and pregnanediol glucuronide (PdG) were predominant steroid metabolites of estrogen and progesterone in feces of most primates and could be used to evaluate ovarian function. These metabolites were determined along with records of genital swelling throughout 3-4 months period from three female Javan Gibbons (Hylobates moloch) maintained in pairing-typed cage at Schmutzer Primate Center, Jakarta (Ullah) and at Taman Margasatwa Taman Sari, Bandung (Donna and Citah). Following methanolic extraction of lyophilized fecal powder, samples were analyzed using enzyme linked immunosorbent assay (ELISA) for E1C and PdG.  In all of the three females observed, both hormone profiles did not indicate any regular cycle of ovarian function even though genital swellings were sometimes observed. In one female (Donna) the hormone patterns showed clear signs of cycle irregularities with extended luteal phase of 40 days and erratic pattern of follicular phase. Of the other two females, no ovarian cycle was found. The data indicate that the fecal steroids analysis is a practical and valuable diagnostic tool for providing reliable information on ovarian function in Javan Gibbon. Factors affected reproductive hormonal profile should be taken in consideration in trying to achieve success in captive breeding program for this species.

Peningkatan Kualitas Spermatozoa Epididimis Kerbau Belang yang Dikriopreservasi dengan Beberapa Konsentrasi Sukrosa (THE QUALITY ENHANCEMENT OF EPIDIDYMAL SPERMATOZOA OF SPOTTED BUFFALO CRYOPRESERVING WITH VARIOUS SUCROSE CONCENTRATIONS)

Jurnal Veteriner Vol 8, No 4 (2007)
Publisher : Jurnal Veteriner

Show Abstract | Original Source | Check in Google Scholar

Abstract

Penelitian dilakukan untuk mengetahui kualitas spermatozoa epididimis kerbau belang selama proses pembekuan (kriopreservasi). Bahan pengencer yang digunakan adalah AndroMed® sebagai kontrol (AM) serta kombinasi AndroMed® dengan 0,2% (S0,2) dan 0,4% sukrosa (S0,4). Hasil penelitian menunjukkan bahwa persentase motilitas spermatozoa epididimis kerbau belang setelah thawing dalam bahan pengencer AM (41%), nyata (P<0,05) lebih rendah dibandingkan dengan S0,2 (46%) dan S0,4 (46%). Demikian pula halnya dengan persentase hidup spermatozoa setelah thawing dalam bahan pengencer AM, S0,2, dan S0,4 secara berturut-turut adalah 52,2; 59,8 dan 60,8% (P<0,05). Sementara itu, tidak terdapat perbedaan nyata (P>0,05) pada persentase membran plasma utuh (MPU) setelah thawing dalam ketiga bahan pengencer. Persentase MPU setelah thawing perlakuan AM, S0,2, dan S0,4 adalah masing-masing 68; 68,8 dan 66,8%. Dapat disimpulkan bahwa penambahan sukrosa sebagai krioprotektan eksternal ke dalam bahan pengencer AndroMed® dapat meningkatkan kualitas spermatozoa epididimis kerbau belang setelah thawing.

Physiological Response of Bligon Buck to Transportation: Relation to Level of Thyroid Hormone

Jurnal Veteriner Vol 11, No 2 (2010)
Publisher : Jurnal Veteriner

Show Abstract | Original Source | Check in Google Scholar

Abstract

Transportated animals may subject to a variety of physical stimuli including metabolism, crowding,noise, handling, isolation, agitation, and extreme temperature .The aim of this study was to determine thechanges of serum T4 and T3 concentration, during animals transportation. Six adult Bligon buck with bodyweight ranging from 26-30 Kg were used in this study. Two weeks prior to the experiment, the animalswere given anthelmintic Albendazole to eliminate egg worm. All animals were fed standard diet in theirpen at 10% of their body weight per head daily and commercial concentrate also given everyday. Freshwater was provided ad libitum. All animals were transported around village for 16 hours starting from18.00 pm until 10.00 am in open small truck (3 x 2 m); eye contact each others would be possible. Bloodsamples were withdrawn from jugular vein using vacutainer tubes containing heparin into 1.5 mL glasstubes, then centrifuged at 500 g for 15 minutes. Plasma was collected to be stored at –20° C. The bloodwere collected every 4 hours from 8 hours before transportation ( at 10.00 am, 14.00 pm and 18.00 pm)until the time of arriving after transportation at 10.00 am. Plasma was harvested and stored at –20° Cuntil T3 and T4 concentrations were measured using ELISA method (enzyme linked immunosorbentassay) product DRG, Germany. The result showed that transportation of Bligon bucks for 16 hours havean affect on level of T4 only (P<0.05) and not for T3 concentration (P>0.05) due to physical stimuli such ascrowding, heat stress, noise, handling would be discarded so that the metabolic process was stable. Duringtransportation, decreasing of T4 levels indicated conversion of T4 to T3 to form active hormone.

Maltosa Mempertahankan Viabilitas Spermatozoa Epididimis Kerbau Belang yang Disimpan dalam Bentuk Cair

Jurnal Veteriner Vol 11, No 2 (2010)
Publisher : Jurnal Veteriner

Show Abstract | Original Source | Check in Google Scholar

Abstract

A study to determine the effect of maltose supplement in andromed based semen extender on theviability of buffalo’s epididymal spermatozoa following 12 and 24 hour storage as liquid semen. Epididymalspermatozoa were collected by combined methods of slicing, flushing and tissue pressure. The spermatozoawas then diluted with three different extenders, i.e Andromed only (A) as control, Andromed + 0.2% w/vMaltose (M1) and Andromed + 0.4% w/v Maltose (M2) as treatments. The percentages of progressivelymotil spermatozoa in the liquid semen in the following treatment of A, M1 and M2 were 48.33%; 53.33%and 55% respectively (after 12h of storage), and 45%; 46.67% dan 45% respectively (after 24h of storage).Besides, the percentage of live spermatozoa in A, M1 and M2 after 12h of storage were 70.33%; 72 % and72.33 % and after 24h of storage were 66.33%; 70% and 70.67 %. In conclusion, the addition of 0.2% and0.4% w/v maltose into Andromed extender could maintain the life of the spotted buffalo epididymalspermatozoa after storage for 4°C up to 24 hours.

Penerapan Cahaya Monokromatik untuk Perbaikan Kuantitas Telur Puyuh (Coturnix coturnix japonica.L)

JURNAL ANATOMI FISIOLOGI Vol 19, No 1 (2011) : Volume XIX, Nomor 1, Maret 2011
Publisher : JURNAL ANATOMI FISIOLOGI

Show Abstract | Original Source | Check in Google Scholar | Full PDF (531.969 KB)

Abstract

The quail’s egg have value of nutrient which similar to the chicken’s egg and the duck’s egg. Various of the programme of the light to improve repair of the quantity and the quality of the quail egg. The objectives of this research were to applied monochromatic light to repair the quality and quantity of the quail egg. Two hundred and seventy female quails (DOQ) were divided into nine treatments of light, with ten replications and three quails in each replication, the treatments were without light, controls with 15 and 25 W, red, green, and blue lights with intensities of 15 and 25 lux. Control treatment used incandescent bulb. The red, green, and blue lights were provided by light emitting diodes (LED). All lights treatment were given for 14 h daily, started from 17.00 to 07.00. Parameters measured were amount of the egg weekly, henday egg production, and quality of the egg consist of the weights of the egg, protein and fat of the egg. The result showed that quails exposed to blue light had higher of the amount of the egg, henday production, and the weights of the egg, while the quality of the egg, such as protein and fat in the egg were not change (P>0,05). Blue light could be used to improve repairing quantities of the quail egg.

Kajian Fisiologis Status Kalsium Puyuh (Coturnix Coturnix Japonica) setelah Pemberian Cahaya Monokromatik

JURNAL ANATOMI FISIOLOGI Vol 18, No 1 (2010): Vol. XVIII, No. 1, Maret 2010
Publisher : JURNAL ANATOMI FISIOLOGI

Show Abstract | Original Source | Check in Google Scholar | Full PDF (425.765 KB)

Abstract

Deposited calsium makes of the eggshell is influnce of the light. The objective of the study was evaluate exposed of monochromatic light to obtain basic information of the physiology aspect of calcium satus in the quail which direct relation with quality of the eggshell. Two hundred and seventy quails were divided into nine treatments of light, with ten replications and three quails in each replication. The treatments were without light, controls with 15 and 25 W, red, green, and blue lights with intensities of 15 and 25 lux. Control treatment used incandescent bulb. The red, green, and blue lights were provided by light emitting diodes (LED). All lights treatment were given for 14 h daily, started from 17.00 to 07.00. Parameters measured were serum calcium concentrations, calcium contents in bone and the eggshell, weights and thichness of the eggshell. The data obtained were analyzed by analysis of variance (ANOVA), and the differences between treatments groups were subjected for Duncan’s Multiple Range Test (DMRT). All of the data analyzed were done GLM (general linear model) procedure in the SAS program. Quails exposed to monochromatic light had higher serum calcium concentrations and calcium contents in bone (P<0,05). Blue light could be used to increase quality of the eggshell.

KADAR ESTROGEN DAN PROFIL OVIDUK PUYUH (Coturnix coturnix japonica) SETELAH PEMBERIAN CAHAYA MONOKROMATIK

JURNAL ANATOMI FISIOLOGI Vol 17, No 2 (2009): Volume XVII, Nomor 2, Oktober 2009
Publisher : JURNAL ANATOMI FISIOLOGI

Show Abstract | Original Source | Check in Google Scholar | Full PDF (323.577 KB)

Abstract

Light plays an important role in the avian life. The present study was designed to evaluate the effects of monochromatic light on serum estrogen concentrations and profile the oviduct, that were the length and weight of the oviduct in the quail. Two hundred and seventy quails were divided into nine treatments of light, with ten replications and three quails in each replication. The treatments were without light,  controls  with 15 and 25 W, red, green, and blue lights with intensities of  15 and 25 lux. Control  treatment used incandescent bulb.  The red,  green,  and  blue lights  were  provided  by   light emitting   diodes  (LED). All lights   treatment   were   given   for   14 h  daily,   started from 17.00 to 07.00. Parameters   measured  were  serum estrogen  concentrations, and  weights  and  lengths  of   the  oviduct  on  weeks 5,  7,  and  9.  Quails   exposed  to  monochromatic   light had  higher  serum  estrogen  concentrations (P<0,05). Quails  exposed to monochromatic light had  better  weights  and  lenghts of  the  oviduct  development. Blue light could be used to increase serum estrogen concentrations and stimulate shell gland development.

FECAL STEROID PROFILE AND GENITAL SWELLING OF FEMALE JAVAN GIBBONS (Hylobates moloch Audebert 1797) MAINTAINED IN INDIVIDUAL CAGE

MEDIA KONSERVASI Vol 12, No 1 (2007): Media Konservasi
Publisher : MEDIA KONSERVASI

Show Abstract | Original Source | Check in Google Scholar | Full PDF (222.389 KB)

Abstract

Pengukuran estron terkonjugasi (E1C) dan pregnanediol glucuronat (PdG) di feses dilakukan pada tiga ekor Owa Jawa (Hylobates moloch AUDEBERT 1797) betina untuk mengevaluasi fungsi ovarium ketiga owa tersebut yang masing-masing dipelihara di kandang individu di Pusat Studi Satwa Primata IPB dan di Taman Margasatwa Ragunan, Jakarta.. Contoh feses dikoleksi secara regular pada jam 07.00-09.00, 5-7 kali seminggu selama 4-9 bulan. Setelah melalui proses pengeringbekuan, contoh feses diekstraksi menggunakan metanol dan asai E1C dan PdG dilakukan dengan enzyme immunoassay (EIA). Profil hormon yang diperoleh menggambarkan adanya satu betina yang bersiklus dengan panjang siklus 21-25 hari. Lama fase folikular yang diperoleh pada betina tersebut bervariasi antara 11-18 hari, dan fase luteal yang relative konstan dengan lama 8-12 hari. Akan halnya dua betina yang lain, tidak diperoleh adanya gambaran pola yang menunjukkan siklus ovarium yang regular. Pembengkakan organ kelamin luar yang berfluktuasi juga hanya teramati pada betina yang bersiklus dengan lama pembengkakan 3-5 hari, dan perdarahan menstruasi terjadi selama 2-3 hari. Hasil yang diperoleh menunjukkan bahwa pengukuran steroid di feses merupakan suatu metoda nonivasif yang dapat diterapkan untuk mengevaluasi fungsi ovarium Owa Jawa. Keadaan fisiologis dari individual betina mungkin menjadi bahan pertimbangan lain dibandingkan tipe perkandangan sebagai faktor yang mempengaruhi profil hormonal.Kata kunci: Owa Jawa, steroid di feses, pembengkakan organ kelamin luar

Co-Authors . Herdis . Safrida . Yulnawati 1Debby Jacqueline Jochebed Rayer, 1Debby Jacqueline Jochebed ACHMAD FARAJALLAH Amelia Hana Andriyanto A Anisa Rahma, Anisa Anshar, Amelia Ramadhani ARIEF BOEDIONO Aryani Sismin Satyaningtijas Asmarani Kusumawati Bambang Kiranadi Bambang Purwantara BEKALANI, YENI KEZIA Cahyadi, Danang Dwi Claude Mona Airin Claude Mona Airin Darusman, Huda Salahuddin Darusman, Huda Salahudin Dirwan Rahman, Dirwan Divo Jondriatno DONDIN SAJUTHI Effendi, E. Mulyati Effendi, Mulyati ERIC HAYES Etih Sudarnika Eva Harlina Ganjar Maulana Nugraha HADI SUKADI ALIKODRA Hadi Sukardi Alikodra Hanadhita, Desrayni Hanadhita, Desrayni Herdis - Husna, Mar’atul Husna, Rina Siti Nurul I WAYAN TEGUH WIBAWAN Isdoni Bustaman Julianto ., Julianto Juliati Gani, Evi Juliati, Evi Kasiyati Kasiati Kasiyati Kasiyati Koekoeh Santoso, Koekoeh LUTHFIRALDA SJAHFIRDI Luthfiralda Sjahfirji Luthfiralda Syahfirdi Maria Fatima Palupi Maria Prihtamala Omega, Maria Prihtamala Meta Levi Kurnia, Meta Levi Mozes R. Tolihere Muhammad Darjat Darulfalah Muhammad Gunawan MUHAMMAD RIZAL Muhammad Zhaahir, Muhammad Muladno . MUSTOFA, MUHAMMAD ADIB Nastiti Kusomorini NASTITI KUSUMORINI Nikki Aldi, Nikki Prawira, Andhika Yudha Pudji Achmadi PUDJI ASTUTI Puji Astuti Purnomo, Rachmatullah Hadi Putri Krida Gita P. Reviany Widjajakusuma Ridi Arif Rivany Widjajakusuma Rosdianto, Aziiz Mardanarian Safrida s Sandra Hapsari Sarmin - Sasmita, Annissa Nuridfi Slamet Widiyanto Srihadi Agungpriyono Supratikno, Supratikno Teguh Budipitojo TUTY LASWARDI YUSUF Umi Cahyaningsih Wasmen Manalu Wisnugroho Agung Pribadi Yulnawati -