Articles

Peningkatan Konsentrasi Testosteron pada Tikus Akibat Paparan Ekstrak Air Biji Pinang Akmal, Muslim; Mahdi, Chanif; -, Aulanni’am
Jurnal Veteriner Vol 11, No 4 (2010)
Publisher : Jurnal Veteriner

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Abstract

Areca catechu which is known in Indonesia as pinang, contains alkaloids such as arecoline, arecaine,arecaidine, arecolidine, guvacine, guvacoline, and isoguvasine. Arecoline has an ability to change gonadmorfofunction, including shape abnormality of sperm. The aim of this research was to find out the prospectof extract betel nut of A.catechu as male anti fertility agents based on its activity to increase the testosteroneconcentration. Animal models used consisted of 5 groups of 2-3 months male rats (Rattus norvegicus,Wistar strain) and induced for 1 week by water extract of betel nut at the dose of 0, 1, 2, 3, and 4 gram/200gram body weight. Testosterone concentration was determined by ELISA technique. The result showedthat extract betel nut of A. catechu is potential source of natural and beneficial male anti fertility agentsas it can increase the testosterone concentration.
PENGARUH TERAPI KURKUMIN TERHADAP KADAR MALONDIALDEHID (MDA) HASIL ISOLASI PAROTIS DAN PROFIL PROTEIN TIKUS PUTIH YANG TERPAPAR LIPOPOLISAKARIDA (LPS) Darwadi, Rizky Prayoga; Aulanni’am, aulanni’am; Mahdi, Chanif
Jurnal Ilmu Kimia Universitas Brawijaya Vol 1, No 1 (2013)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Abstract

Periodontitis merupakan penyakit mulut dan infeksi yang disebabkan oleh bakteri anaerob gram negatif pada rongga mulut. Salah satu bakteri yang diduga sebagai penyebab periodontitis adalah Porphyromonas gingivalis. Merupakan bakteri gram negatif yang bersifat patogen karena membran terluar bakteri tersusun oleh LPS (Lipopolisakarida). LPS diketahui dapat memicu beberapa jenis reaksi peradangan atau infeksi (inflammatory) pada sel makrofag dan sel lainnya yang diikuti dengan terbentuknya radikal bebas. Kurkumin dapat digunakan sebagai terapi karena berfungsi sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui pengaruh terapi kurkumin terhadap MDA dan profil protein pada tikus yang terpapar LPS. Penelitian ini menggunakan tiga kelompok tikus yaitu kontrol, terpapar LPS secara intrasulkuler dan terapi kurkumin. Pengukuran kadar MDA menggunakan metode Thiobarbituric Acid (TBA) dan profil protein menggunakan metode SDS-PAGE. Hasil penelitian menunjukkan paparan LPS mampu meningkatkan kadar MDA secara signifikan (p,0,01) dan juga terdapat beberapa pita protein yang hilang pada metode SDS-PAGE, sebaliknya pada terapi kurkumin kadar MDA memiliki pengaruh menurunkan kadar MDA  dan munculnya pita protein yang rusak.Kata kunci: Lipopolisakarida, Malondildehid, profil protein, radikal bebas
Labelling Antibodi Anti-TPO Menggunakan Alkaline Phosphatase (AP) Untuk Deteksi Autoantibodi TPO Pada Serum Pasien Autoimmune Thyroiditis Disease (AITD) Menggunakan Dot-Blot Nurmahdi, Hilman; Mahdi, Chanif
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 2 (2013)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Abstract

Autoimmune thyroiditis disease (AITD) is an organ specific autoimmune disorder which commonly found in the world. Presence of Thyroid Peroxidase (TPO) enzyme in AITD patient is often used as biomarker in AITD diseases. The aims of this research is done by labelling a polyclonal antibody against autoantibody TPO using Alkaline Phosphatase that used to detect autoantibody TPO in AITD patient using direct Dot Blot method. The protein of Autoantibody TPO is isolated from human AITD sera and has molecular weight of 51.1 kDa. This has been confirmed by western blot using monoclonal antibody standard for TPO. Autoantibody TPO is used as an antigen to produce polyclonal antibody against autoantibody TPO in rabbbit (Oryctolagus cuniculus).  The result of this research shown that autoantibody TPO is immunogenic that could stimulate immune system to produce polyclonal antibody against autoantibody TPO. The highest titer of polyclonal antibody against autoantibody TPO from rabbit sera has optical density of 1,75 using ELISA method. The result of Dot Blot showed that polyclonal antibody which labelled with Alkaline Phosphatase, is able to recognize autoantibody TPO  in patient sera and have the highest colour intensity on the third bleeding.Key words : AITD, Alkaline Phospahatase, autoantibody TPO, Dot Blot, ELISA
OPTIMASI AMOBILISASI UREASE DARI Schizzosaccharomyces pombe MENGGUNAKAN MATRIK Ca-ALGINAT Maharani, Laras Dwi; Prasetyawan, Sasangka; Mahdi, Chanif
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 1 (2013)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Abstract

Urease immobilization is required to be used in repetition The enzyme hydrolize urea to be ammonia and carbondioxide. Urease was isolated from Schizzosaccharomyces pombe and was purified through precipitation by using ammonium sulphate at 30-45% saturation level, followed by dialysis. Urease was immobilized by entrapment method in a matrix of Ca-alginate. The study aimed to determine optimum concentration of Na-alginate and enzym concentration. In this research, Na-alginate concentration (1,5 ; 2 ; 2,5 ; 3 ; 3,5) % (w/v) and variation of urease concentration (0,840; 1,680; 2,520; 3,360; 4,200) mg/mL. Protein content was determined by Biuret reagen and enzyme activity was determined as the amount of mikromol amonia per minute, and resulted amonia was analyzed by Nessler method. The results showed that the optimum condition of immobilization urease is achieved on 3% Na-alginate solution and 2,52 mg/ml urease yielding in 2,52 mg of entrapped enzyme/gram Ca-alginate and the activity of 0,55 Units. Key words: activity, Ca-alginate, Schizzosaccharomyces pombe, urease.
OPTIMASI AMOBILISASI UREASE DARI Schizzosaccharomyces pombe MENGGUNAKAN MATRIK KITOSAN-POLIETILEN GLIKOL Dewi, Hastini Artha; Prasetyawan, Sasangka; Mahdi, Chanif
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 1 (2013)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Abstract

Schizosaccharomyces pombe is yeast that production urease and extraceluller enzyme. Urease can hydrolyze urea to be ammonia and carbondioxide. Free urease is unable to be reused, therefore urease is needed to immobilized in certain matrix. This research studies to determine optimum condition from chitosan concentration and enzyme concentration. Urease was immobilized by entrapment method using matrix chitosan-polyethylene glycol with make a variation to chitosan concentration (1.5; 2; 2.5; 3; 3.5) % (w/v) and make a variation to  urease concentration (0.840; 1.680; 2.520; 3.360; 4.200) mg/mL. Enzyme activity was defined as the amount of mikromol ammonia per minute, and resulted ammonia was analyzed by Nessler method. The results showed that the optimum condition of  immobilization uerase is achieved on chitosan concentration 3% (w/v) and urease concentration of  2.52 mg/mL and urease yielding in 2.36  mg/gram matrix chitosan-polyethylene glycol within the activity of  0.53 Units. Key words: activity, chitosan-polyethylene glycol, Schizosaccharomyces pombe, urease
PENGARUH PENGOBATAN HERBAL SPRAY BERBASIS BIOAKTIF DARI SPIRULINA (SPIRULINA SP.) TERHADAP PROFIL PROTEIN LUKA DAN HISTOLOGI PANKREAS TIKUS (RATTUS NORVEGICUS) TERPAPAR MULTIPLE LOW DOSE STREPTOZOTOCIN (MLD-STZ) Rahma, Kartika; Mahdi, Chanif
Jurnal Ilmu Kimia Universitas Brawijaya Vol 1, No 1 (2014)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Abstract

This research aims to determine the effect of Spirulina sp. extracts in the form of spray on pancreatic tissue histology, and protein profiles of skin wound rats (Rattus norvegicus) induced by Multiple Low Dose streptozotocin (MLD-STZ). The injection of MLD-STZ at a dose of 20 mg/kg BW causes Diabetes Mellitus (DM) type 1. In this study, rats were divided into 3 groups: negative control (no STZ induction), positive control (MLD-STZ-induced), and therapy group (MLD-STZ-induced and therapy). Therapy was given in the form of spray-based herbal bioactive Spirulina sp. with a dose of ± 200 μL right on rat wound 3 times/day during 2 weeks after being DM as a result of MLD-STZ induced. The method used for the analysis of protein is SDS-PAGE and pancreatic tissue histology using hematoxylin-eosin staining methods (HE). The results of the study showed that post-therapy causes the difference of protein band profiles of the three treatment groups, emergence a new protein band with a molecular weight of 93 kDa in skin wounds of diabetic rats, and this protein bands disappear in skin wound rats after treated by herbal spray of Spirulina sp.. In addition, results of histological pancreatic tissue diabetic group has reduction of pancreatic β cells and islet of Langerhans in the cavity width. As well, there is improvement in the therapy group of pancreatic histology.Keywords: diabetes mellitus, pancreatic histology, skin protein bands 
PENGARUH PEMBERIAN HERBAL SPRAY BERBASIS BIOAKTIF DARI SPIRULINA (Spirulina sp.) TERHADAP EKSPRESI iNOS PADA SEL β-PANKREAS DAN AKTIVITAS PROTEASE PADA LUKA TIKUS DM TIPE 1 Agustin, Rachmawati Dwi; Mahdi, Chanif
Jurnal Ilmu Kimia Universitas Brawijaya Vol 1, No 1 (2014)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Abstract

Diabetes mellitus (DM) is a disease caused by disorders of carbohydrate metabolism. DM can also be caused by the destruction of β cells of the islet of Langerhans in the pancreas that functions as an insulin-producing. In patients with DM when injured will be more difficult in the wound healing process. This study aims to determine the effect of Spirulina herbal spray against changes cuts, reduction in iNOS expression in β-cells of the pancreas and protease activity in wound induction results MLD STZ rats (Multi Low Dose Streptozotocin) at a dose of 20 mg / kg for 5 consecutive days in a row. In this study using rats (Rattus norvegicus) were divided into three groups with each group consisting of 5 rats, the three groups are groups of healthy mice with skin wound on his back, a group of diabetic rats with wound and groups diabetic rats with wound and to get therapy of Spirulina herbal spray during 14 days. The results showed that administration of Spirulina herbal spray can reduce the length of incision wounds in diabetic rats from 4,28 cm until 0,5 cm, able to decrease the expression of iNOS in pancreatic β-cells of DM type 1 rats and also able to decrease of 48,23% protease activity in wound DM type 1 rats.Keywords: protease activity, DM type 1, iNOS expression, herbal spray Spirulina, wound.
PENENTUAN WAKTU FERMENTASI OPTIMUM PRODUKSI XILANASE DARI Trichoderma viride MENGGUNAKAN SUBSTRAT KULIT PISANG DAN KULIT MELON DENGAN FERMENTASI SEMI PADAT Hastari, Ayunda Antika; Mahdi, Chanif; Sutisno, Sutrisno
Jurnal Ilmu Kimia Universitas Brawijaya Vol 1, No 1 (2014)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Abstract

Trichoderma viride is a type of mold that most available in land and has several more advantages when compared with other types of soil fungi . This reseach is aimed to know optimum fermentation periode and type of optimum substrate in xylanase production from Trichoderma viride by semi solid fermentation. Optimum fermentation periode and type of optimum substrate determined by measure of enzyme activity and protein content in various fermentation times ( 0 , 12 , 24 , 36 , 48 , 60 , 72 . 84 , and 96 hours ) using melon peels and banana. Xylanase is type of enzyme that able to hydrolyze hemicellulose ( xylan ) into xylose . Determination of enzyme activity begins with a solution of pure xylan in 1 % concentration at pH 5 and a temperature of 60 ° C (optimum conditions). Determination of enzyme activity is determined by measure xilosa in enzimatis reaction with spectrofotometri methode and DNS regen. Protein content determined by spetrophotometry methode with biuret reagen. The results fermentation 60 hour showed optimum activity in the melon peel and banana peel, that is (20,16±8,17) unit dan (19,42±8,00) unit (P<0,01) and protein content (16,86±8,23) mg/mL dan (16,88±4,32) mg/mL (P<0,01).
OPTIMASI AMOBILISASI XILANASE DARI Trichoderma viride MENGGUNAKAN MATRIKS CA-ALGINAT-KITOSAN Mesla, Wifda; Mahdi, Chanif; Sutrisno, Sutrisno
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 1 (2014)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Abstract

ABSTRAK Xilanase bebas tidak dapat digunakan digunakan lebih dari satu kali pemakaian sehingga perlu dilakukan modifikasi enzim dengan metode amobilisasi. Xilanase yang diperoleh dari Trichoderma viride diisolasi dengan sentrifuse dingin pada temperatur 4oC. Xilanase diamobilisasi menggunakan metode penjebakan dalam Ca-alginat-kitosan. Penelitian ini bertujuan untuk mengetahui konsentrasi alginat optimum dan  konsentrasi xilanase optimum dengan cara  menentukan kadar protein dan aktivitas optimum xilanase pada variasi konsentrasi  alginat dan xilanase,  serta menentukan efisiensi pemakaian ulang xilanase amobil. Pada penelitian ini dilakukan variasi konsntrasi alginat (1,5; 2; 2,5; 3; 3,5) % dan variasi xilanase (0,157; 0,209; 0,261; 0,314;0,366) mg/mL. Kadar protein xilanase diuji dengan metode spektrofotometri menggunakan reagen biuret dan aktivitas gula pereduksi dengan reagen DNS. Kondisi optimum amobilisasi xilanase adalah pada konsentrasi alginat 3% dan konsntrasi xilanase 0,314 mg/mL dengan jumlah enzim yang terjebak 0,849 mg dan aktivitas sebesar 17,008 unit (P < 0,01). Efisiensi pemakaian ulang xilanase amobil yang diamobilkan menggunakan matriks Ca-alginat-kitosan adalah hingga lima kali pemakaian ulang dengan efisiensi sebesar 51,28%.   Kata kunci : Xilanase, Trichoderma viride, amobilisasi, Ca-alginat-kitosan, efisiensi    
KARAKTERISASI ENZIM ORGANOFOSFAT HIDROLASE (OPH) DARI PSEUDOMONAS PUTIDA PADA SUBSTRAT KLORPIRIFOS DAN PROFENOFOS Wardani, Kartika Kusuma; Prasetyawan, Sasangka; Mahdi, Chanif
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 1 (2014)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Abstract

Organofosfat hidrolase (OPH) merupakan enzim intraseluler yang diperoleh dengan cara isolasi dari bakteri Pseudomonas putida. Enzim ini mampu mendegradasi pestisida golongan organofosfat. Penelitian ini dilakukan untuk mengetahui kemampuan enzim OPH dalam mendegradasi pestisida golongan organofosfat klorpirifos dan profenofos, serta mengetahui kondisi kerja optimum dan kinetika kimia reaksi enzimastisnya. Enzim OPH dimurnikan melalui fraksinasi bertingkat menggunakan ammonium sulfat dengan fraksi pengendapan 0-45% dan 45%-65%. Uji aktivitas enzim OPH dilakukan dengan mereaksikan enzim dan substrat profenofos dan klorpirifos pada pH optimumnya. Kinetika reaksi enzimatis enzim OPH dengan substrat klorpirifos dan profenofos ditentukan dengan cara mereaksikan enzim OPH dengan substrat profenofos dan klorpirifos dengan variasi konsentrasi 2-10 ppm, kemudian ditentukan Vmaks dan KM. Hasil penelitian menunjukkan aktivitas enzim spesifik untuk substrat klorpirifos adalah 0,017 unit sedangkan untuk substrat profenofos 0,024 unit. pH optimum OPH untuk substrat klorpirifos yaitu pH 7,5 sedangkan pH optimum untuk substrat profenofos yaitu pH 8,5. Parameter kinetika reaksi enzimatis enzim OPH untuk substrat klorpirifos Vmax= 1,34 x 10-3 µg. mL-1. menit-1, KM = 2,17 mg/L dan profenofos Vmax = 1,87 x 10-3 µg. mL-1. menit-1, KM =3,08 mg/L. Hasil analisa dengan menggunakan pola rancangan acak lengkap (RAL) menunjukkan bahwa perlakuan pH berpengaruh nyata terhadap aktivitas OPH (p<0,05).   Kata kunci: aktivitas enzim spesifik, OPH, organofosfat, Pseudomonas putida