MARIA ENDO MAHATA
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Characterization of Extracellular Chitinase from Bacterial Isolate 99 and Enterobacter sp. G-1 from Matsue City, Japan MAHATA, MARIA ENDO; DHARMA, ABDI; RYANTO, IRSAN; RIZAL, YOSE
Microbiology Indonesia Vol 2, No 1 (2008): April 2008
Publisher : Indonesian Society for microbiology

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Abstract

One hundred and twenty isolates of chitosanase producing bacteria were screened from water and soil from localies around Matsue city, Japan. In previous experiments, four isolates (isolates 96, 97, 99, and 100 strain ) were analyzed for their chitosanase characteristics, and one of the isolates (99) was detected as being both a chitosanase and a chitinase producer. Characteristics of the chitinase enzyme were analyzed in this study. Chitinase from bacterial isolate 99 showed higher activities compared to that Enterobacter sp. G-1 (isolated from water in Matsue city, Japan), the activity was 0.039 U/ml and the specific activity was 0.56 U/mg protein, while those from Enterobacter sp. G-1 were 0.029 U/ml and 0.48 U/mg protein respectively. Chitinase from isolate 99 was stable in a pH range between 4-7, while that from Enterobacter sp. G-1 was stable in pH range 3-7. Optimum pH of the chitinase produced by isolate 99 was 5 whereas the chitinase from Enterobacter sp. G-1 it was pH 7. Chitinase from isolate 99 was stable at temperature 20-60°C, while that from Enterobacter sp. G-1 at 20-50°C. Chitinase secreted by isolate 99 showed optimum temperature of 50°C while chitinase from Enterobacter sp. G-1 was optimal at 40°C. Several ions (Fe2+, Ba2+, Co2+) increased the activity of the enzyme from isolate 99 whereas Ca2+ and Co2+ increased activity of the Enterobacter sp. G-1 chitinase..
PENINGKATAN KUALITAS CAMPURAN KULIT PISANG DENGAN AMPAS TAHU MELALUI FERMENTASI DENGAN Phanerochaete chrysosporium DAN Neurospora crassa SEBAGAI PAKAN TERNAK Nuraini, Nuraini; Mahata, Maria Endo; Djulardi, Ade
JURNAL PETERNAKAN Vol 11, No 1 (2014): Februari 2014
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/jupet.v11i1.2324

Abstract

Banana peel can be used as an alternative feed based on the potential availability and nutritional. The experiments were conducted to improve the nutrient quality of Banana Peel and Tofu Waste mixture (BPTW) by using Phanerochaete chrysosporium and Neurospora crassa. This substrate consists of banana peel 70% and tofu waste 30%. The experiment was performed in 3 treatment completely randomized design (CRD) with 6 replicates. The treatments were : A= fermentation BPTW by Phanerochaete chrysosporium and Neurospora crassa (1:1), B = fermentation BPTW by Phanerochaete chrysosporium and Neurospora crassa (2:1), and C = fermentation BPTW by Phanerochaete chrysosporium and Neurospora crassa (1:2). Measured variables were Crude Fiber (CF), Crude Protein (CP), Nitrogen Retention and CF digestion. The result of the research showed that the treatment affected significantly (p<0.01) reduced CF and increased CP, nitrogen retention and CF digestion. In conclusion, fermentation by Phanerochaete chrysosporium and Neurospora crassa (2:1) was the best treatment for improving banana peel and tofu waste nutrient quality
POTENSI DAN PENINGKATAN KUALITAS NUTRISI BUAH TOMAT (Lycopersicon esculentum) AFKIR SEBAGAI PAKAN TERNAK UNGGAS Handayani, Ulvi Fitri; Mahata, Maria Endo; Rizal, Yose; Suliansyah, Irfan; Wizna, Wizna
JURNAL PETERNAKAN Vol 15, No 1 (2018): Februari 2018
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/jupet.v15i1.4279

Abstract

Salah satu hal yang penting dalam menentukan kualitas produk ternak adalah bahan pakan. Kualitas produk ternak akan dipengaruhi oleh asupan nutrisi yang di berikan. Buah tomat afkir merupakan salah satu bahan pakan yang istimewa karena tingginya kandungan likopen didalamnya. Likopen sudah terkenal baik memiliki kemampuan sebagai antioksidan dan menurunkan kandungan kolesterol. Akan tetapi likopen ini masih berada dalam matrik jaringan tomat dan berbentuk trans likopen yang sulit untuk dicerna dan diserap oleh tubuh. Sehingga dalam paper ini menyajikan sejauh mana perkembangan penelitian pemanfaatan buah tomat yang tinggi likopen sebagai pakan unggas. Serta membuka lebih luas bagaimana meningkatkan nilai guna likopen tomat dalam produk ternak unggas.