I Gusti Ngurah Mahardika
Laboratorium Biomedik Fakultas Kedokteran Hewan,Universitas Udayana

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STRUKTUR GENETIK DAN FILOGENI YELLOWFIN TUNA (THUNNUS ALBACARES) BERDASARKAN SEKUEN DNA MITKONDRIA CONTROL REGION SITOKROM OKSIDASE I PADA DIVERSITAS ZONE BIOGEOGRAFI Wijana, I Made Sara; Mahardika, I Gusti Ngurah
Bumi Lestari Journal of Environment Vol 10, No 2
Publisher : Udayana University

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Abstract

Genetic structure and phylogeni of 37 sequences control region DNA mitochondrial cytocrome oxidase I of yellowfin tuna (Thunnus albacores) have been downloaded from GeneBank and analised using Maximum Likehood (ML), Pairwise Genetic Distance and Bootstrapping Phylogeni Model of Kimura 2 Parameter. The result shows that the corrected value of the data was 5,5% and the mean of genetic distances was 3,7 %, where the shortest distance was 0.0% and the longest was 5%. The genetic distances with the out group (Thunnus obesus) ranged between 7,8 % – 9,8 % and with the Thunnus thynus ranged between 10,4% – 12,5%. The value of bootstrap phylogeny of 37 sequences of yellowfin tuna was less than 50%. All those results shows that there was no significant genetic differences of 34 samples sourced from Philipines and 3 from Spain based on sequence region DNA mitochondrial cytocrome oxidase I.
Survei Penyakit Porcine Reproductive and Respiratory Syndrome pada Peternakan Babi di Bali (SEROLOGICAL SURVEILLANCE OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME IN PIGGERIES IN BALI) Suartha, I Nyoman; Anthara, I Made Suma; Wirata, I Wayan; Sari, Tri Komala; Dewi, Ni Made Ritha Krisna; Narendra, I Gusti Ngurah; Mahardika, I Gusti Ngurah
Jurnal Veteriner Vol 14, No 1 (2013)
Publisher : Jurnal Veteriner

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This study aimed to determine the presence and burden of Porcine Respiration and ReproductiveSyndrome (PRRS) virus in pig farms in Bali. A total of 305 sera samples were collected from 10 intensivepig farms and backyard piggeries located in eight districts, in Bali. The PRRS antibody and the virus wasdetected using enzyme linked immunosorbent assay (ELISA) and transverse reverse polymerase chainreaction (RT-PCR), respectively. The results showed that, generally the average percentage of positiveswine anti-PRRS antibody was 13.4%, 14.3%,  and 11.7% in the backyard farms and commercial farms,respectively. Whereas, the detection rate of PRRS virus was 8.9% (15.3% and 5.6% in the backyard farmand commercial farms, respectively). It was concluded that PRRS virus is endemic in pigs, in Bali.Vaccination, management, biosafety, and quarantine  should be implemented to prevent the economicloss due to PRRS.
REVALENSI PORCINE CIRCO VIRUS SECARA SEROLOGIS PADA PETERNAKAN BABI DI BALI Suartha, I Nyoman; Anthara, I Made Suma; Wirata, Wayan; Krisna Dewi, Ni Made Ritha; Narendra, I Gusti Ngurah; Mahardika, I Gusti Ngurah
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.488 KB) | DOI: 10.21157/j.ked.hewan.v9i1.2790

Abstract

Penelitian ini bertujuan mengetahui seroepidemiologi infeksi porcine circo virus (PCV-2) dua pada peternakan babi di Bali. Pada penelitian ini sampel yang dianalisis sebanyak 295 sampel. Sampel berasal dari peternakan babi rakyat sebanyak 98 dan dari peternakan babi intensif sebanyak 197. Sampel berasal dari delapan kabupaten dari sembilan kabupaten yang ada di Bali. Deteksi antibodi dilaksanakan dengan uji enzyme-linked immunosorbent assay (ELISA) dan deteksi virus dilakukan dengan polymerase chain reaction (PCR). Hasil penelitian menunjukkan bahwa seroprevalensi antibodi anti-PCV-2 adalah 84,1%, dengan sebaran di peternakan rakyat dan peternakan intensif masingmasing sebesar 70,4 dan 91,2%. Semua peternakan babi intensif menunjukkan antibodi positif. Prevalensi virus PCV-2 di seluruh Bali sebesar 1,7% dengan sebaran pada peternakan rakyat peternakan intensif masing-masing sebesar 3,1 dan 1,0%. Dari hasil penelitian disimpulkan bahwa infeksi PCV-2 pada peternakan babi di Bali bersifat endemis.