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GENETIC DIVERSITY OF OLIVE RIDLEY LEPIDOCHELYS OLIVACEA ASSOCIATED WITH CURRENT PATTERN IN CENDRAWASIH BAY, PAPUA Bahri, Samsul; Atmadipoera, Agus S.; Madduppa, Hawis H.
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 9 No. 2 (2017): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1322.338 KB) | DOI: 10.29244/jitkt.v9i2.19307

Abstract

Cendrawasih Bay is habitat for olive ridley (Lepidochelys olivacea) which is directly adjacent to the Pacific Ocean. The presence and the diversity of L. olivacea in Cendrawasih Bay has been threatened. Genetics can be the key to conservation because they play an important role in maintaining population and recovering from damage. This study aims to determine the genetic diversity of L. olivacea and its association to the current pattern in the waters of Cendrawasih Bay. Samples of L. olivacea (n=20) were collected in Kwatisore (n=8) and Yapen Island (n=12) in Cendrawasih Bay waters from August 2015 to December 2016. The molecular analysis was based on 791-bp fragment of D-Loop on the non-coding region gen. The current pattern analysis was performed through INDESO data and visualized by using Ferret software. Phylogenetic analysis showed that 2 groups of L. olivacea from a total of 2 haplotypes, whose population from Kwatisore was dispersed in two haplotypes, while the population from Yapen Island was only dispersed in one haplotype. Populations from Kwatisore showed higher variations than populations from Yapen Island. The current pattern analysis suggests that the two study sites, Kwatisore and Yapen islands are different. Both populations are only connected by the Northwest monsoon currents period that indicates a little geneflow between this populations. Thus causing differences variation between Kwatisore and Yapen Island populations genetically. Keywords: genetic diversity, current pattern, coral triangle, olive ridley
Laju Dekomposisi Serasah Daun di Ekosistem Bakau Pulau Kelong, Kabupaten Bintan Dharmawan, I Wayan Eka; Zamani, Neviaty P.; Madduppa, Hawis H.
Oseanologi dan Limnologi di Indonesia Vol 1, No 1 (2016)
Publisher : Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/oldi.2016.v1i1.8

Abstract

Hutan bakau di bagian tenggara Pulau Kelong memiliki cadangan karbon yang banyak dalam tegakan dan sedimen yang mengindikasikan produktivitas primer kawasan yang tinggi. Dekomposisi serasah daun, sebagai bagian dari produktivitas kawasan, menghasilkan unsur hara esensial sederhana yang dimanfaatkan untuk menopang pertumbuhan bakau. Tujuan penelitian ini adalah untuk mengetahui laju dekomposisi serasah daun di setiap zona penelitian, yaitu zona darat (Landward, L), tengah (Middle zone, M), dan dekat laut (Seaward, S) yang dibedakan berdasarkan kelimpahan jenis tumbuhan dan parameter in situ. Penelitian dilaksanakan pada bulan Maret–Mei 2015 di kawasan bakau Pulau Kelong, Kabupaten Bintan. Proses dekomposisi diamati pada enam interval waktu, yaitu 2, 4, 7, 14, 28, dan 42 hari dengan menggunakan kantong jaring yang berisi 10 g daun basah yang diletakkan di permukaan substrat. Hasil penelitian menunjukkan bahwa L merupakan zona dengan persentase dekomposisi tertinggi (56,34 ± 20,58%) dibandingkan dengan M dan S yang berturut-turut sebesar 51,59 ± 15,46% dan 51,09 ± 8,70%. Laju dekomposisi serasah paling tinggi di zona L yaitu 1,40 ± 0,82 g·m-2.hari-1 yang berbeda nyata dari zona M dan S, berturut-turut sebesar 0,83 ± 0,89 g·m-2.hari-1 dan 0,78 ± 0,70 g·m-2.hari-1.  Analisis koefisien laju dekomposisi (k) menunjukkan bahwa laju dekomposisi di dalam kawasan tergolong tinggi (k > 0,01). Laju dekomposisi memiliki korelasi dengan kelimpahan spesies bakau, nilai salinitas air, dan suhu tanah
Phylogenetic of tuna fish (Thunnus spp.) in North Mollucas Sea, Indonesia Akbar, Nebuchadnezzar; Aris, Muhammad; Irfan, Muhammad; Baksir, Abdurrachman; Surahman, Surahman; Madduppa, Hawis H.; Kotta, Raismin
Jurnal Iktiologi Indonesia Vol 18, No 1 (2018): February 2018
Publisher : Masyarakat Iktiologi Indonesia (MII )

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.845 KB) | DOI: 10.32491/jii.v18i1.370

Abstract

The tuna fish (Thunnus spp.) is highly migratory and commercial tuna fishery. The fish tuna abudance supported ocea-nography and geography condition in North Mallucas Sea. The fishery targets catch increase on fish tuna provided a view of the need for assessment of phylogenetic tuna. The study was conducted to infer the phylogenetic in North Mollucas Sea. The research method was PCR-Sequensing. Moleculer analysis included extraction, Polymerase Chain Reaction (PCR), electrophoresis and DNA sequencing in control region mtDNA locus. Phylogenetic reconstructed with Neigbor joining with Kimura 2-parameter model using MEGA5. The result showed that four clade (bigeye, yellowfin, alalunga and skipjack). Genetic distance between bigeye with yellowfin was (0.084), bigeye with alalunga (0.163), ye-llowfin with alalunga (0.174), bigeye with skipjack (0.294), skipjack with alalunga (0.312) and yellowfin with skipjack (0.297). The overall result showed significant genetic different. That information explain about one populations species tuna. The tuna phylogeography unlimitedin geographic distributions. AbstrakIkan tuna (Thunnus spp.) adalah ikan pelagis yang memiliki kemampuan ruaya dan nilai komersial. Kondisi oseanogra-fis dan letak geografis mendukung kelimpahan stok sumber daya ikan tuna di Perairan Maluku Utara. Aktifitas penang-kapan yang meningkat memberikan pandangan perlu adanya pengkajian filogenetik ikan tuna. Penelitian ini bertujuan untuk memperoleh informasi filogenetik ikan tuna di perairan Maluku Utara. Metode yang digunakan adalah metode PCR-Sekuensing pada lokus mtDNA control region. Analisis molekuler meliputi ekstraksi, Polymerase Chain Reaction (PCR), elektroforesis dan sekuensing DNA. Rekonstruksi pohon filogenetik dengan metode Neighbor joining dengan model evolusi Kimura 2-parameter dilakukan menggunakan aplikasi MEGA5. Hasil penelitian menemukan empat clade spesies ikan tuna yang berbeda (tuna mata besar, sirip kuning, alalunga, dan cakalang). Jarak genetik tuna mata besar (Thunnus obesus) dengan sirip kuning (Thunnus albacares) adalah 0,084; tuna mata besar dengan tuna alalunga (Thunnus albacore) adalah 0,163; tuna sirip kuning dengan tuna alalunga sebesar 0,174; tuna mata besar dengan caka-lang (Katsuwonus pelamis) adalah 0,294; cakalang dengan tuna alalunga adalah 0,312; dan tuna sirip kuning dengan cakalang adalah 0,297. Semua hasil menunjukkan perbedaan genetik signifikan. Namun dapat dijelaskan bahwa spesies tuna berasal dari satu keturunan. Filogeografi tuna tidak memiliki batas distribusi yang nyata spesies.
STUDY ON BIOROCK® TECHNIQUE USING THREE DIFFERENT ANODE MATERIALS (MAGNESIUM, ALUMINUM, AND TITANIUM) Zamani, Neviaty P.; Bachtiar, Ramadian; Madduppa, Hawis H.; Adi, Jhoni Wahyu; Isnul, Jeddah; Iqbal, Muhamad; Subhan, Beginer
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 2 No. 1 (2010): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.332 KB) | DOI: 10.29244/jitkt.v2i1.7858

Abstract

Biorock® technique is the earliest methods to rehabilitate the damage of coral reef ecosystem. Its improvement is need to carry on since it is subject to some inhibitions in particular the dependence of expensively-imported Titanium (Ti) as the anode materials. The main purpose of this research was to find the best anode material as a possible subtitution which can be economically and easily to apply in Indonesia. Therefore we compared Titanium (Ti) with two other potential anodes material (Magnesium-Mg, and Aluminum (Al). The laboratory study was carried out for two days period (48 hours) in the stagnant sea water aquaria. Four aquarium tanks were treated by different electric current treatment (1 Ampere, 2 Ampere, 3 Ampere, and 5 Ampere, respectively). The reduction of electrode weigh (anode and cathode) was measured. During the experiment, water quality (i.e. pH, and salinity) and mineral waters (calcium) were collected every 6 hour in 48 hours. The solid form of calcium carbonate was analyzed using XDS (X-ray Dispersion Spectrophotometer). The accretion rates on cathode and anode decay were compared between anodes which were treated by different electrical current. Based on the study, three anodes (aluminum, magnesium and titanium) showed different respond. Titanium and Magnesium anode showed electric current affected accretion rate (P < 1), while for aluminum anode showed no differences on treatments.Keywords: mineral accretion, Biorock®, anode, coral reef rehabilitation, accretion rate, anode decay rate, mineral uptake