IAN JAMES MACFARLANE
2School of Biotechnology and Biomolecular Sciences, The University of New South Wales, NSW 2052, Australia

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THE GENETIC RELATIONSHIPS OF GREVILLEA HYBRIDS DETERMINED BY RAPD MARKER PHARMAWATI, MADE; MACFARLANE, IAN JAMES
HAYATI Journal of Biosciences Vol. 20 No. 4 (2013): December 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (488.987 KB) | DOI: 10.4308/hjb.20.4.196-200

Abstract

Grevillea (Proteaceae) is native to Australia and is known as garden plant. The flowers have high diversity colors, which range over orange, yellow, pink, red and green. It is a large genus, consisted of 357 species. This genus is economically important and there are hundreds of Grevillea hybrids with beautiful color and shape of flower.  Information of the genetic relationships of Grevillea hybrids is not available. Ten Grevillea hybrids were used in this study to evaluate the genetic relationships between hybrids and with their parents. PCR-RAPD technique was employed in this study with seven RAPD primers. The dendrogram of Grevillea hybrids was performed using Neighbor-Joining analysis based on genetic distance. The analysis revealed that ?Moonlight?, ?Caloundra Gem?, and Little Pink Willie? were clustered with Grevillea banksii. The three hybrids were hybrid of G. banksii and other Grevillea species.  ?Superb? was closely related to ?Robyn Gordon? as they are hybrid of G. banksii and G. bipinnatifida.