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Seberapa Efektifkah Pemberantasan DBD dengan Insektisida? Lusiyana, Novyan
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 6, No 2, (2014)
Publisher : Faculty of Medicine

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol6.Iss2.Art1

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Wolbachia Sebagai Alternatif Pengendalian Vektor Nyamuk Aedes SP. Lusiyana, Novyan
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 6, No 3, (2014)
Publisher : Faculty of Medicine

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Schistosomiasis Husnawati, Eka; Lusiyana, Novyan
Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 7, No 3, (2016)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol7.Iss3.art6

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Schistosomiasis, also known as bilharzia, is a zoonosis disease caused by blood flukes (trematodes) of the genus Schistosoma sp. There are a few species that can infect humans and animals, including Schistosoma aponicum, Schistosoma mansonia, Schistosoma hematobium, Schistosoma intercalatum Schistosoma guineensis and Schistosoma mekongi.1 This disease is endemic in 78 countries, in which 52 of them are countries with moderate-high level of endemicity. Schistosoma sp are very difficult to eliminate, because the transmission is highly influenced by evironmental, habitual, parasitic, vector, and host factors.
KOLONISASI NYAMUK Aedes aegypti MENGGUNAKAN TEHNIK MEMBRAN ARTIFISIAL DI LABORATORIUM Lusiyana, Novyan; Tri Cahyani, Mira Sagita
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 6, No 3, (2014)
Publisher : Faculty of Medicine

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Latar belakang  Kolonisasi nyamuk  Aedes aegypti  di laboratorium  dapat menggunakan AMF (Artificial Membrane Feeding). Membran artifisial yang sering digunakan seperti parafilm M, latek kondom, dan kulit mencit.  Penggunaan membran artifisial  mempengaruhi  kemampuan reproduksi nyamuk  Ae. aegypti.    Tujuan  Mengetahui pengaruh penggunaan membran artifisial parafilm M, latek kondom, dan kulit mencit  terhadap persentase nyamuk dewasa yang mampu menghisap darah, rerata jumlah telur dan persentase daya tetas telur nyamuk  Ae. aegypti di laboratorium.   Metode Penelitian ini merupakan penelitian eksperimental murni. Nyamuk  Ae. aegypti  sebanyak 30 ekor (8 replikasi) dimasukkan ke dalam gelas  rearing  yang telah diberi AMF berisi darah dengan membran yang berbeda  jenisnya yaitu, parafilm M, latek kondom dan kulit mencit. Blood feeding dilaksanakan selama 60 menit, setelah 60 menit kemudian dihitung jumlah nyamuk yang mampu menghisap darah. Nyamuk yang telah menghisap darah kemudian dimasukkan ke dalam gelas rearing secara individu untuk bertelur. Jumlah telur yang diproduksi dihitung setelah 7 hari paska pemberian umpan darah. Telur kemudian direndam dengan air selama 7 hari untuk pengamatan daya tetas. Hasil dianalisis dengan Uji One way ANNOVA.     Hasil Persentase  nyamuk  Ae. aegypti  yang mampu menghisap darah menunjukkan perbedaan signifikan (p=0,000) antara membran parafilm M (88,33%), latek kondom (45,42%), dan kulit mencit (86,66%).  Jumlah telur  per ekor nyamuk betina  juga menunjukkan beda signifikan (p=0,002) antara membran parafilm M (51,63), latek kondom (50,53), dan kulit mencit (53,65),  sedangkan persentase daya  tetas  telur  juga menunjukkan perbedaan signifikan (p=0,02) antara membran parafilm M (99,94%), latek kondom (99,91%), dan kulit mencit (99,96%).   Kesimpulan Membran  artifisial  kulit mencit  menunjukkan hasil yang lebih baik sehingga lebih direkomendasikan sebagai metode rearing nyamuk Ae. aegypti di laboratorium.   Kata kunci: Artificial Membrane Feeding (AMF), blood feeding, membran parafilm M,  latek kondom dan kulit mencit
THE EFFECT OF ANTICOAGULANT IN BLOOD MEAL SOURCE ON THE AEDES AEGYPTI REPRODUCTIVE ABILITY IN LABORATORY Lusiyana, Novyan; Mulyaningsih, Budi; Umniyati, Sitti Rahmah
Tropical Medicine Journal Vol 3, No 2 (2013): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.018 KB) | DOI: 10.22146/tmj.5872

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ABSTRACTIntroduction: Aedes aegypti is one of the major vectors of dengue hemorrhagic fever (DHF) that can be reared in laboratory. Artificial membrane feeding (AMF) assay is used as a simulated host to blood feeding mosquitoes in laboratory.Objectives: The purpose of this study are to investigate the effect of heater and the most widely used anticoagulant of K3EDTA, heparin and sodium citrate on blood feeding sucsses, feeding rate, fecundity, hatchability, preadult development and survival rate of Aedes Aegypti colonies maintained by AMF system compared to direct human feeding.Methods: The system consisted of AMF with parafilm membrane which are warmed and not warmed by a waterbath. Human blood samples were used to feed Aedes aegypti using AMF. The number of eggs were counted seven days after feeding after mosquitoes laid the eggs. Every eggs were hatched in a 500 mL of rearing glass to evaluate the hatchability and preadult development. Survival rate is evaluated after blood feeding until 30 days. Data was analyzed using one-way ANOVA and paired t-tests and a p value <0.05 considered as significantly difference.Results: Blood feeding success of Aedes aegypti was not significantly differ when offered blood meal using anticoagulant heparin with heater (82.22%) compare to that of control groups (81.67%) (p=0.917). There was a significant difference in feeding rate (p=0.000), fecundity (p=0.000), hatchability (p=0.000) between groups. All results were higher in heparin than K3EDTA and sodium citrate, but in pre adult development and survival rate K3EDTA showed better result than that of control, heparin and sodium citrate groups. So this anticoagulant was acceptable for maintenance of laboratory colonies of Aedes aegypti.Conclusion: We conclude that heater can increase the blood feeding sucsses. The K3EDTA, heparin and sodium citrate can affect the feeding rate, fecundity, hatchability, and preadult development, but do not affect survival rate. Heparin can be used for routine colonization of laboratory strain of Aedes aegypti with  AMF assay.Keywords: Anticoagulants, artificial membrane feeding, reproductive ability, survival rate
CASE-BASED LEARNING MENINGKATKAN CLINICAL REASONING PADA BLOK PENYAKIT INFEKSI TROPIS MAHASISWA FAKULTAS KEDOKTERAN UNIVERSITAS ISLAM INDONESIA Sulistyoningrum, Evy; Lusiyana, Novyan
Jurnal Penelitian Pendidikan Vol 35, No 2 (2018): Oktober 2018
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpp.v35i2.15253

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Case-based learning (CBL) is a newer modality of teaching healthcare, combining medical theory and practices. Many researchs reported effectivity of CBL to improve student-centered learning. The aim of this study is to evaluate how CBL improved students’ clinical reasoning compared with other method such as problem-based learning. An interventional research involved 4th grade medical students receiving PBL and CBL methods supervised by a tutor or instructure. An alternative method combining CBL and structured role play (in an Integrated-Patient Management method) also performed using clinical scenario with medical practice setting. The result showed that student received CBL had better clinical reasoning indicated from better miniquizz result compared with PBL students (p < 0,05, Mann Whitney U test. Students exposed with CBL had final MCQ and essay exam grade compared with unexposed students. Students exposed with CBL also had better clinical skill indicated from Objective Structure Clinical Examination (OSCE) result (p < 0,05, independent t test). Students’ acceptance level on CBL method is 79, 4%. It is concluded that compared with PBL, CBL is more effective in improved students’ performance, learning outcomes and clinical reasoning and also had high acceptance among medical students.
Peningkatan Literasi Masyarakat terhadap Orang Dengan Skizofrenia (ODS) di Desa Sindumartani Yogyakarta Rafik, Abdur; Febrianti, Yosi; Lusiyana, Novyan
Jurnal Abdimas Madani dan Lestari (JAMALI) Volume 01, Issue 02, September 2019
Publisher : UII

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The Results of Basic Health Research from Ministry of Health of the Republic in Indonesia places Yogyakarta as one of the provinces with the highest prevalence of schizophrenia after Bali, and Sindumartani Village is one of the villages with the highest number of People with Schizophrenia (PWS) in Yogyakarta. The presence of negative stigma from the community and even family members of PWS is one of the crucial obstacles to the PWS healing process. The stigma and low social awareness towards PWS are thought to hamper the process of enriching ODS? social cognition. Our activities try to provide counseling and education to non-PWS members groups and PWS? family members so that they can be well literated for schizophrenia with all the characteristics and handling. In addition to the counseling, we educate them by forming a healthy mental alert cadre within the community. In the period after the implementation of the activities, the results showed an increase in communities? literacy in schizophrenia and a decrease in the prevalence of recurrence in PWS. To improve the sustainability of results, further therapy such as vocational therapy is recommended.?Hasil Riset Kesehatan Dasar (RISKESDAS) Kementerian Kesehatan Republik Indonesia menempatkan Yogyakarta sebagai salah satu provinsi dengan prevalensi skizofrenia tertinggi di Indonesia setelah Bali, dan Desa Sindumartani adalah salah satu desa dengan jumlah Orang dengan Skizofrenia (ODS) tertinggi di Yogyakarta. Adanya stigma negatif dari masyarakat dan bahkan anggota keluarga ODS menjadi salah satu penghambat krusial proses penyembuhan ODS. Stigma dan kesadaran sosial yang rendah terhadap ODS disinyalir mmenghambat proses pengembangan kognisi sosial pada ODS. Pengabdian masyarakat ini mencoba melakukan penyuluhan dan edukasi terhadap kelompok masyarakat non-ODS dan anggota keluarga ODS agar mereka dapat terliterasi secara baik akan skizofrenia dengan segala karekteristik dan penanganannya. Selain dengan penyuluhan, edukasi dilakukan dengan membentuk kader siaga sehat jiwa dalam kelompok masyarakat. Dalam kurun waktu setelah pelaksanaan kegiatan, hasil menunjukkan adanya peningkatan literasi masyarakat terhadap skizofrenia dan penurunan prevalensi kekambuhan pada ODS. Untuk meningkatkan kesinambungan hasil, terapi lanjutan dalam bentuk vokasional dianjurkan untuk dilakukan.
Optimalisasi problem based learning dengan media pembelajaran video case untuk meningkatkan ketercapaian tujuan belajar blok penyakit infeksi tropis Lusiyana, Novyan
Refleksi Pembelajaran Inovatif Vol 1, No 1 (2019): Volume 1 Nomor 1 Tahun 2019
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/rpi.vol1.iss1.art1

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Latar belakang: Tutorial merupakan salah satu bentuk kegiatan belajar dalam Problem based learning (PBL). Selama ini pencapaian tujuan belajar tutorial dalam blok penyakit infeksi tropis belum maksimal karena masih menggunakan skenario berbentuk paper case. Penggunaan video case dalam tutorial blok penyakit infeksi tropis diharapkan mampu meningkatkan ketercapaian tujuan belajar dalam blok penyakit infeksi tropis. Tujuan: Membandingkan rerata ketercapaian ujuan belajar, rerata nilai ujian tulis antara mahasiswa dengan skenario tutorial video case dan paper case dan akseptabilitas mahasiswa terhadap penggunaan skenario video case. Metode: Penelitian ini merupakan penelitian kuantitatif non-eksperimental dengan rancangan cross-sectional. Penelitian ini melibatkan 137 mahasiswa pendidikan dokter FK UII angkatan 2012 dan 2011. Mahasiswa angkatan 2012 mendapatkan 3 skenario video case sedangkan angkatan 2011 menggunakan 3 skenario paper case. Kemudian dilihat rerata ketercapaian ujuan belajar, rerata nilai ujian tulis dan akseptabilitas skenario video case. Penelitian ini juga membandingkan hasil yang didapat antara mahasiswa angkatan 2012 dan 2011. Hasil dan pembahasan: Persentase ketercapaian tujuan belajar angkatan 2012 pada ketiga skenario berturut turut adalah 100%; 100%; dan 100%, sedangkan pada angkatan 2011 adalah 89,9%; 88,8%; dan 58,5%. Ketercapaian tujuan belajar angkatan 2012 lebih tinggi dan berbeda secara bermakna (p=0,000) dibandingkan dengan angkatan 2011. Persentase jawaban benar ujian akhir angkatan 2012 (71,7%) lebih tinggi dibandingkan angkatan 2011 (40,3%) dan berbeda bermakna (p=0,000). Sebanyak 98,33% mahasiswa menilai video case bermanfaat, 95% meningkatkan pemahaman, 85% pelaksanaannya telah baik.