Articles

The Comparison of Streptococcus agalactiae Isolated from Fish and Bovine using Multilocus Sequence Typing LUSIASTUTI, ANGELA MARIANA; SEEGER, HELGA; INDRAWATI, AGUSTIN; ZSCHÖCK, MICHAEL
HAYATI Journal of Biosciences Vol 20, No 4 (2013): December 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (654.76 KB) | DOI: 10.4308/hjb.20.4.157-162

Abstract

Multilocus sequence typing (MLST) has greater utility for determining the recent ancestral lineage and the relatedness of individual strains. Group B streptococci (GBS) is one of the major causes of subclinical mastitis of dairy cattle in several countries. GBS also sporadically causes epizootic infections in fish. The aim of this study was to compare the evolutionary lineage of fish and bovine isolates in relation to the S. agalactiae global population as a whole by comparing the MLST profiles. Twenty S. agalactiae isolates were obtained from dairy cattle and fish. PCR products were amplified with seven different oligonucleotide primer pairs designed from the NEM316 GBS genome sequence. Clone complexes demonstrated that bovine and fish isolates were separate populations. These findings lead us to conclude that fish S. agalactiae is not a zoonotic agent for bovine. MLST could help clarify the emergence of pathogenic clones and to decide whether the host acts as a reservoir for another pathogenic lineage.
Pengaruh Ekstrak Daun Sirih Merah (Piper crocatum) Terhadap Profil Darah Dan Kelulushidupan Ikan Mas (Cyprinus carpio) Yang Diinfeksi Bakteri Aeromonas hydrophila Syahida, Intan Eska Amalia; Sarjito, Sarjito; Prayitno, Slamet Budi; Lusiastuti, Angela Mariana
Journal of Aquaculture Management and Technology Vol 2, No 4 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

The study aimed was to know the influence of Piper crocatum exstract on the blood profile included total erythrocyte, total leukocyte, differential leukocyte (DL), phagocytosis indeks (IF) and survival rate of common carp (Cyprinus carpio) infected by Aeromonas hydrophila and the best concentration of P. crocatum for imunostimulatory common carp of infected A. hydrophila. The research was conducted at Research and Development of Freshwater Aquaculture, Bogor. The methods used in this research is experimental methods by using random design complete (RAL) with 4 treatments and three replicates. The research was conducted at Research and Development of Freshwater Aquaculture, Bogor. The treatments of different addition of P. crocatum exstract on fish feed, 0 gr/kg feed (treatment A), 5 gr/kg feed (treatment B), 10 gr/kg feed (treatment C), 15 gr/kg feed (treatment D). The treatnent was given 21 days. The research results obtained additional P. crocatum exstract significantly different effect (P0,05) on percentage of netrofil (2%) and survival rate of common carp (C. carpio) infected by A. hydrophila (40%), the best concentration of P. crocatum on feed for immunostimulant common carp of infected A. hydrophila, the result showed the best dose 5 g/kg feed
Pengaruh Ekstrak Daun Sirsak (Annona muricata) Terhadap Profil Darah Dan Kelulushidupan Ikan Mas (Cyprinus carpio) Yang Diinfeksi Bakteri Aeromonas hydrophila Nurjannah, R. Dewi Dharina; Prayitno, Slamet Budi; Sarjito, Sarjito; Lusiastuti, Angela Mariana
Journal of Aquaculture Management and Technology Vol 2, No 4 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Carp is a freshwater species that is easily cultivated. One of the constraints in cultivating carps (C. carpio) is diseases that ultimately caused mass mortality. The disease that generally affects carp is Motile Aeromonas Septicemia (MAS) caused by Aeromonas hydrophila. Soursop leaves is one of the herbs that can be used as an ingredient to control fish diseases because it contains chemicals that acts bacteriocide. The purpose of this research was to determine the influence and the best dose of soursop leaf extract in feed to the blood profile and survival of carp infected by A. hydrophila. The treatments of this research were addition of soursop leaf extract on feed with 4 different dosages and 3 replicates. The treatments were A (0 g/kg diet), B (5 g/kg diet), C (10 g/kg diet) and D (15 g/kg diet). The result showed that soursop leaf extract in feed of treatment B (5 g/kg diet) significantly different (P0.05) on total erythrocyte (0.83×106 cells/mm3), differential leukocyte include the percentage of monocytes (7.67%), neutrophils (8.67%), lymphocytes (83.67%), phagocytic activity (37%) and survival rate (50%). It can be conclude that soursop leaf extract at dosage 5g/kg was able to stimulate immune response of carp, it was characterized by an increased total leukocytes of carp infected by A. hydrophila.
Pengaruh Ekstrak Daun Sirsak (Annona muricata L) Terhadap Profil Darah dan Kelulushidupan Ikan Lele Sangkuriang (Clarias gariepinus Var. Sangkuriang) yang Diinfeksi Bakteri Aeromonas Hydrophila Kurniawan, Kurniawan; Prayitno, Slamet Budi; Sarjito, Sarjito; Lusiastuti, Angela Mariana
Journal of Aquaculture Management and Technology Vol 2, No 4 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

The main problem in catfish farms is mass mortality coused by Motile Aeromonas Septicemia (MAS) by Aeromonas hydrophila. Natural ingredients that are currently being developed is soursop leaf. It was because soursop leaf contains acetogenin compounds that wishes compared to other plant parts. An acetogenin compound has the benefit of as an antibacterial. The purpose of this research was to determine the influence and best dose of sourshoup leaf extract in feed to the blood profile and survival rate of catfish infected by A. hydrophila. The treatment in this study were the addition of soursop leaf extract on feed with different dosage and 3 replicates. The treatments were A (0 g/kg diet), B (5 g/kg diet), C (10 g/kg diet), D (15 g/kg diet). The result showed that soursop leaf extract in feed of treatment B on the best dose (5 g/kg diet) was total erythrocytes(1,51 x 106 sel/mm3), total leukocytes (8,00 x 103 sel/mm3), limphocytes (92%), monocyte (8%), neutrophils, phagocytic index (75%), and survival rate (93%). It can be concluded that dose of 5 g/kg diet was the best dosage of soursop leaf extract in the feed that was able to stimulate the immune response of catfish, it was characterized by an increased erythrocytes, total leukocytes, differetial leukocyte (limphocytes and monocyte) of catfish infected by A. hydrophila.
Kandidat Vaksin Potensial Streptococcus agalactiae untuk Pencegahan Penyakit Streptococcosis pada Ikan Nila (Oreochromis niloticus) (POTENTIAL VACCINE CANDIDATE OF STREPTOCOCCUS AGALACTIAE FOR PREVENT STREPCOCOCOSIS ON NILA TILAPIA (OREOCHROMIS NILOTICUS) Hardi, Esti Handayani; ., Sukenda; Harris, Enang; Lusiastuti, Angela Mariana
Jurnal Veteriner Vol 14, No 4 (2013)
Publisher : Jurnal Veteriner

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Abstract

The effectiveness of Streptococcus agalactiae vaccine in tilapia (Oreochromis niloticus) was evaluatedfor prevention of streptococcal disease. The vaccine was prepared using formalin-killed whole cell andconcentrated extracellular products/ECP (62.3 and 55.8 kDa) of â-haemolityc isolate and 62.3; 55,8 and51.8 kDa protein of  non-haemolityc ECP of S. agalactiae.  Vaccination and challenged (103 colony-formingunits (CFU)/fish of â-haemolityc and 105 CFU/fish of non-haemolityc S. agalactiae) trial was conducted byintraperitonial (IP) injection into fish with average body weight of 15 g.  Fish were vaccinated with wholecell, ECP and mixed (whole cell and ECP) vaccine.  Tilapia vaccinated with whole cell of â-haemolitycisolate had a relative percent survival (RPS) rates higher than those of ECP â-haemolityc vaccine. However,fish  vaccinated with mixed (whole cell and ECP) of â-haemolityc has a better protection rates as comparedto those of two type of S. agalactie infection. Whereas those vaccinated with mixed (whole cell non-haemolitycand ECP of â-haemolityc) vaccine has protection rate of 79% from â-haemolityc and 42% from non-haemolitycinfection.  Tilapia vaccinated with whole cell of non-haemolityc was only able to protect fish from non-haemolityc infection and was unable to protect fish from other types.  Tilapia vaccinated with ECP non-haemolityc had a worse RPS than others vaccines in which mix whole cell and ECP vaccine of non-haemolitychad a protection 50-56% from  S. agalactiae infection. Whereas vaccinated with mixed (whole cell â-haemolityc and ECP of non-haemolityc) vaccine showed a better to protect from â-haemolityc than non-haemolityc infection.  It showed thatvaccination with mixed (whole-cell and extracellular product)  vaccineof S. agalactiae â-haemolityc  was more effective to protect tilapia against Streptococcosis.
APLIKASI VAKSIN Streptococcus agalactiae UNTUK PENCEGAHAN PENYAKIT STREPTOCOCCOSIS PADA BUDIDAYA IKAN NILA (Oreochromis niloticus) [Application of Streptococcus agalactiae vaccine to prevent streptococcosis on tilapia culture, Oreochromis niloticus] Taukhid, Taukhid; Lusiastuti, Angela Mariana; Sumiati, Tuti
BERITA BIOLOGI Vol 13, No 3 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.857 KB) | DOI: 10.14203/beritabiologi.v13i3.662

Abstract

The research with the aim to know the effectivity (yield gap) of the application of Streptococcus agalactiae vaccine (pure whole cell) in prevention of streptococcosis on tilapia (Oreochromis niloticus) culture has been carried out. The isolate of S.agalactiae – N14G was used as a master seed on vaccine production. Priming vaccination was administered by immersion method, and booster vaccination was taken th place two months latter by oral method. Challenge test at the lethal dose (LD50) against active bacteria was done at 14 days post booster vaccination, and observation was taken place for 14 days post artificial infection. The results of the research showed that the highest survival rate and relative percent survival (RPS) was found in group treated with Streptovac vaccine (S. agalactiae and A. hydrophila combination) (65.58% and 35.36%) followed by S. agalactiae vaccine (52.08% and 10.01%). The lowest survival rate was found in control group (46.75%). The result of confirmation effectivity of the vaccines by challenge test in the laboratory showed that the highest survival rate and relative percent survival (RPS) was found in S. agalactiae vaccine (50.00% dan 37.50%) followed by Streptovac vaccine (40.00% and 25.00%), and the lowest survival rate was found in control group (20.00%). Vaccination is better than the non vaccinated.
OPTIMASI FREKUENSI PEMBERIAN VITAMIN C PAD A PAKAN KOMERSIAL UNTUK PENGENDALIAN PENYAKIT KOI HERPES VIRUS (KHV) PADA IKAN MAS (Cyprinus carpio Linn.) Taukhid, Taukhid; Lusiastuti, Angela Mariana; Suryadi, Kusumasari; Rosidah, Rosidah; Setiadharma, Gunawan
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (529.665 KB) | DOI: 10.14203/beritabiologi.v10i3.749

Abstract

The research with objective to understand optimization frequency of supplemented ascorbic acid (microencapsulated vitamin C CFC-90) feeding to control the Koi Herpes Virus (KHV) disease infecting common carp has been done in Fish Disease Laboratory Fishes were reared in plastic container (80 litres), with density of 20 fish sized 10 gram in average. The treatments were: (A) daily application, (B) three daily application, (C) five daily application, and (D) without vitamin C as a control. Examined fishes were challenged to KHV infection after the 21 days rearing period by cohabitation method for 2 weeks. Observations been done on behaviour, clinical signs and mortality of fishes. The results showed that the highest survival rate was found on the application o vitamin C given every 3 days (50.0%); followed by every day (12.5%), every 5 days (7.5%), and the lowest was found on contro group (1.3%). Control techniques in the case of KHV carp populations through the provision of vitamin C immunostimulatory conducted regularly since well before the existence of KHV infection provides the best protective level.
PENGGUNAAN VAKSIN Aeromonas hydrophila: PENGARUHNYATERHADAP SIANTAN DAN IMUNITAS LARVA IKAN PATIN (Pangasionodon hypophthalmus) Lusiastuti, Angela Mariana; Hadie, Wartono
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (892.062 KB) | DOI: 10.14203/beritabiologi.v10i2.1967

Abstract

Aeromonas hydrophila is a pathogen that often causes considerable losses in the area of freshwater fish fanning. Vaccination is one way to simulate parent catfish make specific immunity. Specific immunity generated by the parent will be forwarded through the oocytes produced during a certain time span. The aim of this research was to know the effect and the effectivity of using hydrovac vaccine with and without the complete adjuvant. This research was done on Patin fish Pangasionodon hypophthalmus whose givng Hydrovac 0.4 ml/kg of body weight. The comparation between complete adjuvant and vaccine was 1:1. Injection was done by intra peritoneal for three mothers each with and without complete adjuvant. Injection was done at gonad maturity level II. The result showed that antibody were positively detected on mother serum which used adjuvant or not. On larva stage, antibody was detected until four weeks old. While on 2 weeks old of larva, the concentration of titer antibody was very high and raised the dilution of 1: 2048. Survival rate of juvenile which their mother got a vaccine raised 93%, was better than 73%-63% using mother without vaccine. Booster immersion of hydrovac vaccine could give preferably at the end of three weeks old or in the beginning of fourth weeks old of larva.
Specific Immune Response Kinetics and Mortality Patterns of Tilapia Oreochromis niloticus on Post-Cocktail Vaccination Period against the Infection of Aeromonas hydrophila and Streptococcus agalactiae Sukenda, Sukenda; Sumiati, Tuti; Nuryati, Sri; Lusiastuti, Angela Mariana; Hidayatullah, Dendi
Journal Omni-Akuatika Vol 13, No 2 (2017): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.583 KB) | DOI: 10.20884/1.oa.2017.13.2.279

Abstract

ABSTRACTFish vaccination aims to induce a specific immune response indicated by an increase of antibodies in vaccinated fish. However, in accordance with time the presence of antibodies will continue to decline. The purpose of this study was to determine the kinetics of specifik immune response and trend mortality against Aeromonas hydrophila and Streptococcus agalactiae on tilapia following vaccination with cocktail vaccine. Fish vaccinated through immersion for 30 minutes in a solution of diluted vaccine. Challenge test was performed for three periods, on day 22, 50, and 78 post-vaccination, fish were challenged with single infection of A. hydrophila 108 cfu. mL-1 and S. agalactiae 104 cfu. mL-1 and co-infection of both bacteria by intraperitoneal. During rearing, the blood fish were taken for determining of serum antibodies, and its  measured by ELISA. The results showed that the concentration of specific antibodies vaccinated fish were significantly higher than the control. The basal antibody levels of A. hydrophila before vaccination were higher than S. agalactiae with OD of 0.104 and 0.069 respectively. The maximum  antibody  response  was  reached  within  70  days  of  the  A. hydrophila OD= 0.264 and 56 days against S. agalactiae OD= 0.188. The mortality rate in the control group was significantly higher than vaccinated on all types and each challenge test period. The trend of mortality due to a single infection of A. hydrophila and co-infections occur more quickly than by S. agalactiae. Lowest mortality occurred in the vaccinated group at 50 day tested challenge.Keywords: kinetics antibody, Aeromnas hydrophila, Streptococcus agalactiae, Oreochromis niloticus
Frekuensi Penambahan Probiotik Bacillus sp. Dan Staphylococcus sp. Pada Media Pemeliharaan Benih Ikan Lele Dumbo (Clarias gariepinus) Untuk Ketahanan Terhadap Aeromonas hydrophila Yustiati, Ayi; Lusiastuti, Angela Mariana
Jurnal Perikanan Kelautan Vol 6, No 2(1) (2015): Jurnal Perikanan dan Kelautan
Publisher : Fakultas Perikanan dan Ilmu Kelautan Universitas Padjadjaran

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Abstract

 Penelitian ini bertujuan untuk mengetahui frekuensi pemberian probiotik multispecies Bacillus sp. dan Staphylococcus sp. yang tepat pada media pemeliharaan sehingga dapat meningkatkan kelangsungan hidup benih ikan lele dumbo (Clarias gariepinus) yang terinfeksi Aeromonas hydrophila. Metode yang digunakan dalam penelitian ini adalah metode eksperimental Rancangan Acak Lengkap (RAL) dengan empat perlakuan dan tiga ulangan. Perlakuan tersebut adalah A (tanpa penambahan probiotik Bacillus sp. dan Staphylococcus sp. dan tanpa pemberian Aeromonas hydrophila (kontrol negatif)), B  (tanpa penambahan probiotik Bacillus sp.  dan  Staphylococcus sp.  dan  pemberian Aeromonas hydrophila 103 CFU/ml (kontrol positif)), C (penambahan probiotik Bacillus sp.   103 CFU/ml dan Staphylococcus sp. 103  CFU/ml diberikan setiap hari serta penambahan Aeromonas hydrophila 103  CFU/ml) dan D (penambahan probiotik Bacillus sp. 103  CFU/ml dan Staphylococcus sp. 103  CFU/ml diberikan setiap dua hari sekali serta penambahan Aeromonas hydrophila 103 CFU/ml). Parameter yang diamati adalah tingkat kelangsungan hidup ikan uji  selama masa pemeliharaan pertumbuhan biomassa, Feed  Convertion Ratio  (FCR), differensial leukosit, indeks fagositik dan kualitas air. Hasil penelitian menunjukkan bahwa probiotik perlakuan D (Bacillus sp. 103  CFU/ml dan Staphylococcus sp.               103 CFU/ml) pada media pemeliharaan benih ikan lele dumbo yang diberikan setiap dua hari sekali menghasilkan kelangsungan hidup tertinggi 93,33%, proporsi monosit 30% dan aktivitas fagosit 77,5% dibandingkan dengan perlakuan A, B dan C. Kata kunci :   Bacillus sp., Staphylococcus sp., Aeromonas hydrophila, lele dumbo, Clarias gariepinus