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Kemampuan Bakteri Probiotik pada medium Soygurt Kacang Merah (Phaseolus vulgaris L) dengan konsentrasi skim yang berbeda dalam menurunkan kadar kolesterol secara in vitro

BIOMA Vol. 09, No. 1, Tahun 2007
Publisher : BIOMA

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Abstract

Pemanfaatan strain strain bakteri

Optimasi Produksi Inulinase isolat P 12 pada Tepung Umbi Dahlia ( Dahlia variabilis Wild ) dengan Variasi Konsentrasi Nitrogen Organik dan Waktu Inkubasi

Bioma Vol. 12, No. 1, Tahun 2010
Publisher : Bioma

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Abstract

Efforts to address health problems mainly related to the digestive tract is by consuming one prebiotic, egfruktooligosakarida (FOS). FOS is a prebiotic that one species can be produced from the hydrolysis of inulin usinginulinase enzyme. Isolate P12 is an isolate that has been proven to have high inulinase activity on standard mediuminulin production. Inulinase production increase can be done by adding a source of organic nitrogen in the form ofyeast extract in medium. The results indicate that the best on the concentration of nitrogen concentration P2 (Yeastextract 0.25%) with the activity of 0.7983 IU, while the best 12-hour incubation time with the activity of 0.7899 IU.Likewise for the best interaction P2 T2 treatment with inulinase activity of 0.9025 IU.

Kemampuan Bakteri Asam Laktat Dalam Menghambat Pertumbuhan dan Produksi Aflatoksin B2 Aspergilllus flavus

Bioma Vol. 09, No. 2, Tahun 2007
Publisher : Bioma

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Abstract

Aflatoxins are highly toxic secondary metabolies produced during the growth of several fungi, especiallyAspergillus flavus. AFB1 and AFB2 one of them which contaminates a wide variety of food and feed causing serioushealth problem when consumed by human or animals. This research was aimed to study the potency of Lactic AcidBacteria (LAB) in the inhibition of Aspergillus flavus growth and the production of Aflatoxin B2. Three species ofLAB i.e. Lactobacillus delbrueckii , L. fermentum, L. plantarum were investigated for their potential in inhibitingand degradation of Aflatoxin B2 as well as inhibiting fungal growth. The trial was designed into three variations ofeach isolate by challenging the fungal culture, before fungal inoculation, at the same time as fungal inoculation andafter fungal inoculation. It was found that all the three species of LAB are potential microorganism to inhibitfungal growth as indicated by the reduction of the dry weight of fungal mycelia compared with control.Quantification of Aflatoxin B2 showed that L. fermentum gave the strongest degradation of Aflatoxin B1 during 15days incubation, then followed by L. plantarum and L delbruekii with a reduction rate of 0,,2408 ppm, 0,3373 ppm,0,6393 ppm respectively, compared with control these are significantly different. These result conclude thatAflatoxin B2 can be degraded or prevented to be produced by A. flavus by applying LAB.

EFEKTIVITAS PENGAWETAN SECARA OZONISASI DAN IDENTIFIKASI TERHADAP KONTAMINASI KAPANG PADA KACANG TANAH (Arachis hypogaea L.)

Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Peanut (Arachis hypogaea L.) is a potential plant protein source with a relatively cheap price, so that it can be consumed by almost all the community. Post-harvest handling of peanuts especially at the stage of the marketing needs to be done properly, because improper treatment can lead to contamination by fungi. As a result, it can reduce the quantity and quality of the product and can endanger the health of consumers. This study aims to determine the Ozonisation Preservation and Identification of Mold Contamination In Peanuts. Mold was isolated using the direct method (direct method) and dilution. Identification of molds were done macroscopically and microscopically. Identification was done by using Potato Dextrose Agar (PDA) and Malt Extract agar (MEA) as selective media. The result of Ozonisation Preservation with 3.5 ppm concentration showed that peanuts that had been exposed with ozone on first day containing the amount of molds lower than on the day 7, 14, 21, and 28. The result of mold identification on peanuts consisted of one genus Aspergillus with predominant isolate was A. flavus as many as 25 colonies. Keywords: peanut (Arachis hypogaea L.), ozonation, identification, mold

PRODUKSI INULINASE DAN KECEPATAN PERTUMBUHAN SPESIFIK ISOLAT KHAMIR DUCC Y-015 PADA BERBAGAI KONSENTRASI TEPUNG UMBI DAHLIA

Jurnal Akademika Biologi Vol. 2 No. 3 Juli 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Inulin is an abundant compound in the nature after starch. Hydrolytic enzyme called inulinase is able to catalyse hydrolysis reaction of inulin polysaccharides into fructose and fructooligosaccharide (FOS). Yeast isolateDUCC Y-015 is one of the microbes that is able to produceinulinase. The action of inulinase enzyme associated with the growth of the isolate. Specific growth rate is one of the measurement of growth parameters; itis defined as the increase in cell biomass per unit time in the growth phase of the microbes. This study aims to examine theinulinase production and specific growth rate of yeast isolate DUCC Y-015 on the medium with various concentrations of dahlia tuber flour. The measurement of growth was done by weighing the cell dry weight and inulinase activity test was done by calculating the reduction sugar using DNS method. The used concentrations of dahlia tuber flourin the medium were 2%, 3%, 4% and 5%. Inulinase activity in each treatment was 0,111 IU/ml, 0,147 IU/ml, 0,113 IU/ml and 0,119 IU/ml, respectively, whereas the specific growth rate was 0,0062 hour-1, 0,0081 hour-1, 0,0077 hour-1, 0,0052 hour-1, respectively. The resultswere tested using the Mann-Whitney Test and theKruskal-Wallis Test. According to the results, the most optimuminulinase production and specific growth rate (µ) of yeast isolate DUCC Y-015 on dahlia tuber flour medium was treated with 3% concentration of dahlia tuber flour.Keywords: Dahlia Tuber Flour, Yeast Isolate DUCC Y-015, Specific Growth Rate, Inulinase Activity

Uji Kemampuan Produksi Fruktooligosakarida (FOS) dari Kelompok Aspergillus niger DUCC

Bioma Vol. 15, No.1, Tahun 2013
Publisher : Bioma

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Abstract

Prebiotic is a food supplement that can not be digested in the human’s gut, it can stimulate the growth of beneficial microorganisms in the intestine and improve human’s health. FOS is a fructan type of oligosaccharide which is have a potential as a natural prebiotic, it  can be produced by several microorganisms, including A. niger group. The aim of this research was to examined the FOS production of   three isolates of A. niger group from DUCC collection (i.e. DUCC F123, DUCC F129 and DUCC F102). FOS production was determined by measuring the reducing sugar using  DNS method. The result showed that all isolates have the capability in producing  FOS suspected kestose with the degree of polymerization 3.545; 3.215; 3.049 respectively.   Keywords:FOS, fructosyltransferase, Aspergillus niger, prebiotic

Penambahan Filtrat Tepung Umbi Dahlia (Dahlia variabilis Willd.) sebagai Prebiotik dalam Pembuatan Yoghurt Sinbiotik

Bioma Vol. 15, No.2, Tahun 2013
Publisher : Bioma

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Abstract

Yoghurt is a fermented milk product that can provide beneficial effects to the health of consumers. Increasing the quality of yoghurt can be made by combining the benefits of probiotic with prebiotic to produce sinbiotic yoghurt. Inulin is a prebiotic that found in many dahlia tuber (Dahlia variabilis Willd.). Pure inulin were expensive, so the alternative is using dahlia tuber flours filtrate as prebiotic ingredients. This study aimed to examine the addition of Dahlia Tuber Flours Filtrate (DTFF) as a prebiotic in sinbiotic yoghurt making using Lactic Acid Bacteria (LAB) Lactobacillus bulgaricus, Streptococcus termophilus dan Lactobacillus acidopilus as probiotic. This research using Completely Randomized Design (CRD) with 4 concentration treatments DTFF 0%, 3%, 6% and 9% with 3 replications. Observed variables consist of the number of total LAB, lactic acid level, pH and organoleptic attributes (aroma, flavor, consistency and impression). Data the number of total LAB, lactic acid level and pH values ​​were analyzed by ANOVA, continued by Duncan test, while the organoleptic data was analyzed by Mann-Whitney U test with 95% significant level. The results showed that the addition of DTFF significantly affect the increasing of total LAB, lactic acid level, organoleptic assessment and decreasing pH of sinbiotic yoghurt. Sinbiotic yoghurt with 3% of DTFF concentration is most preferred by panelists and required the criteria of Indonesian National Standard (SNI) for yoghurt with enough likely aroma, sour taste/specify and enough thick texture with total LAB 2,4 x 108 CFU/ml, 1.3% lactic acid levels and 4.2. pH. Keywords : prebiotic, probiotic, inulin, dahlia tuber, sinbiotic yoghurt, lactobacillus bulgaricus, streptococcus termophilus, lactobacillus acidopilus

Chromosomes and Mitotic Cell Division Phase In Onion Roots After 24 Hours Acetoorcein Soaking Time

Bioma Vol. 14, No. 2, Tahun 2012
Publisher : Bioma

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Abstract

Onions (Allium cepa) are usually used in vitro to assess effect of chemical subtances by allowing developing roots to come into contact with substances to be tested. Acetic orcein staining of onion chromosomes has remained a standard method of preparation. However, aceto-orcein stain is corrosive and poisonous chemical substances since it containing oxidising agents such as organic peroxides, the toxic substances which are are cyanides, acid corrosives agents, and  also radioactive substances.  This research study mitotic activity in the roots of onion plants to determine the effects of soaking time of aceto orcein dye on actively dividing root cells. A series of several root tip from each bulb was harvested were soaked in 1, 3 and 24 hours on aceto-orcein stain and processed further for cytological studies by the aceto-orcein squash technique. The research  was carried out to study the effect of to mitotic index and chromosomal aberration  on  onion root. It will determine the percentage of cells that are undergoing mitosis. The squash techniques were used to observe mitosis in the tip of onion root cells during actively mitotic division cells time. Mitotic divisions occur in several phases, consist of prophase, metaphase, anaphase, telophase and interphase. Experiment were repeated six times for every soaking time. The data was analyzed by using T-Test. The result showed that various duration of soaking time significantly influenced the  reduction of mitotic index value. The lowest mitotic index  on glyphosate concentration 100 ppm i.e. 10. 73% and 7.19% for the duration  of soaking time 3 and 6 hours. The highest mitotic index on  glyphosate concentration 0 ppm i.e.  37.71% and 32.76% for the duration  of soaking time 3 and 6 hours. The result also showed that the chromosomal aberration were increased significantly. The lowest  chromosomal aberration  obtained i.e. 2.55% and 2.96% for the duration of aceto orcein soaking time 1, 3 and 24 hours. The highest chromosomal aberration obtained i.e. 21.71% and 36.26% for the duration of soaking time 1,3 and 24 hours. The type of chromosomal aberration were abnormal prophase, stickiness, bridge, abnormal anaphase, clumping chromosome, c- metaphase,  change of nucleous  shape and size. At 72h, their cytotoxic effects on the root tips showed strong growth retardation in high concentrations of all the wastewaters. Compared to the control, treatment with the wastewaters resulted in root growth inhibition with EC50 values of 35, 50 and 62% for bottling, rubber and brewery effluents respectively, and decrease in mitotic index with increasing concentration for all samples and these were statistically significant (p<0.05). Chromosomal aberrations induced in the onion root tip cells were mostly sticky chromosomes and bridges. Chromosomes with disturbed spindles and fragments were also present in appreciable amounts. Based on the EC50 values, the bottling wastewater was most toxic, followed by rubber effluent while effluents from the brewery were least toxic. The findings in this study indicate that there are toxic chemicals present in the wastewaters which are responsible for the observed genotoxic effects on the onion root tip cells. The study also reveals that the Allium test is a useful and reliable tool for the genotoxicity screening of industrial effluents which could be employed by environmental managers before these effluents are finally discharged into the environment.     Key words: chromosomes, onion roots, acetoorcein

PRODUKSI PIGMEN OLEH ISOLAT KAPANG HASIL ISOLASI DARI ANGKAK KOMERSIAL DI SEMARANG PADA SUMBER N DAN pH BERBEDA

Jurnal Akademika Biologi Vol. 2 No. 4 Oktober 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The development of the food processing industry led to the use of dyes have also increased, especially the type of synthetic dyes that can be harmful to consumers because of its toxicity. Natural dyes to be one of the alternatives used in the field of food. One of the natural dyes is widely used as a food coloring that is red yeast rice. Red yeast rice is rice that is overgrown by the mold Monascus sp. that produces pigment. This study aims to obtain pure isolates of red yeast rice molds that are in Semarang and knowing the growth and production of red pigment in the fungi isolates the different source of N and pH. The treatment is done by growing PDB (potato dextrose broth) in the medium with treatment medium pH 3,5,7 and 9 as well as optimization of the nitrogen source Ammonium chloride 1 %, Ammonium Nitrate 1 %, and Peptone 1 %. Analysis of pigments using a spectrophotometer with a wavelength (λ) of 500 nm and analysis of dry cell weight mycelia (g/l). The results showed the highest pigment concentration at treatment pH 7 with 0.812 absorbance value and the highest value of the cell dry weight at pH 7 is 1.232 g/l. Results of optimization with different nitrogen sources showed the highest pigment levels in the addition of a nitrogen source Ammonium Chloride 1 % to the value of 0.821 absorbance and dry weight of most cells are in Ammonium Nitrate is 2.556 g/l. Keywords: Pigment, Angkak, Isolate Fungus, pH, Nitrogen.

Eksplorasi Mikroorganisme Termofil Indigenous Dari Sumber Air Panas Gedongsongo Sebagai Penghasil Ensim Termostabil

Berkala Bioteknologi Volume 1 Nomor 1 April 2018
Publisher : Berkala Bioteknologi

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Abstract

ABSTRAK Mikroorganisme termofil merupakan suatu sumber untuk mendapatkan ensim ektraseluler termotoleran yang penting dalam indusri. Isolasi bakteri termofil dari dua sampel air yang berasal dari sumber air panas geotermal di Gedongsongo Jawa  Tengah pada lokasi yang telah ditentukan ini bertujuan untuk mendapatkan isolat-isolat bakteri termofil indigenous  yang potensial menghasilkan ensim amilase, protease dan selulase termotoleran. Isolasi dilakukan dengan medium selektif Skim Milk Agar, CMC Agar dan Nutrien Amilum Agar. Inkubasi dilakukan pada suhu 55oC. Koloni yang menunjukkan aktivitas ensim diisolasi ke dalam medium Nutrient Agar. Uji aktifitas ensim dilakukan pada medium yang mangandung skim milk untuk protease, amilum untuk amilase dan CMC untuk selalulase, inkubasi dilakukan pada 55oC selama 72 jam. Pengamatan morfologi dan sifat gram isolat juga dilakukan. Penelitian ini menghasilkan  4 isolat bakteri proteolitik termotoleran yang terdiri dari 2 isolat gam positif kokus dengan indeks proteolitik tertinggi  1,40;   2 isolat gram negatif kokus dengan indeks proteolitik tertinggi 3.5. Enam isolat bakteri amilolitik termofil  terdiri dari 2 isolat gram positif kokus, 2 isolat gram negatif kokus, satu  isolat gram positif batang dan satu  isolat gram negatif batang . Indeks amilolitik berkisar dari 1,29-2,50.   Tujuh bakteri selulolitik termofil terdiri dari 5 isolat gram positif kokus dengan indeks selulolitik tertinggi 5,0;  satu  isolat kokus gram negatif, dengan indeks selulolitik 7,5 satu isolat batang gram positif dengan indeks selulolitik 3,0. Kata kunci: termofil, bakteri, amilase, protease, selulase