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Journal : Jurnal Kedokteran Hewan

THE ROLE OF GUAVA FRUIT FILTRATE ON MAINTAINING THE PRE-FREEZING AND POST-THAWED QUALITY OF BALI BULL SPERMATOZOA Sumadiasa, I Wayan Lanus; Lukman, Lukman; Rodiah, Rodiah
Jurnal Kedokteran Hewan Vol 12, No 2 (2018): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.362 KB) | DOI: 10.21157/j.ked.hewan.v12i2.8801

Abstract

This study aimed to determine the role of guava fruit filtrate (GFF) on maintaining the pre-freezing and post-thawed quality of Bali bullspermatozoa in tris-egg yolk (TEY) extender. The bovine semen was collected using artificial vagina twice a week (n= 10). Ejaculate sampleswere divided into four tubes and each tube was respectively added the following diluents: TEY, TEY + 5% GFF, TEY + 10% GFF, and TEY +15% GFF. The diluted semen was filled into the straws (0.25 mL) and cooled in refrigerator at 4° C for 2.5 hours. The samples were kept onvapor phase of liquid nitrogen (N2) (-120° C) for 10 minutes prior to be stored in liquid phase (-196° C). The quality of pre-freezing and postthawedspermatozoa in straw sample (i.e. motility, viability, and abnormality) was evaluated under light microscopy at 400x magnification. Datawere analysed statistically. The percentage of motility and viability both on pre-freezing and post-thawed process were significantly higher insemen diluted with TEY + 10% GFF compared to control (TEY), TEY + 5% GFF, and TEY + 15% GFF. Addition of 10% GFF into tris-egg yolkextender play role for maintaining the quality of pre-freezing and post-thawed Bali bull spermatozoa.
Co-Authors Achmad Haikal, Achmad Achmad Supriyanto Agus Dwi Anggono Amsal Amri Andi Alimuddin Unde, Andi Alimuddin Anto Tri Susilo, Andri Anzalena, Ria Aptensi, Fenny Ardian, Ani Ardian, Ani Ari Susandy Sanjaya, Ari Susandy Arif Wijaya Arskadius A, Mohd. Asbah, Asbah Aswin Fitriansyah, Aswin Burhanuddin Burhanuddin Casanova, Gusti Chrismadha, T Dadi, Sri Deden Ibnu Aqil, Deden Ibnu Dila, Ratna Djuwita, Puspa Dwi Anggraini Fadma, Mike Wahyu Fakhrudin, Muhammad Fibriani, Fini Fitriani agustina Fransiskus Randa Giri Sucahyo, Yudho Gupron, Ahmad Hamidah Hamidah Harry Dhika, Harry Hartono Hartono Helfi Nasution Herawati Jaya Herman Yasin Hidayati Hidayati Hijril, Hijril Hujair A.H. sanaky Hustarna Hustarna, Hustarna I Wayan Lanus Sumadiasa, I Wayan Lanus Ilham Ilham Imam Sunoto, Imam Indra Sensuse, Dana Indrawan, Donny Ishartiwi Ishartiwi Joko Marwoto Karjiyati, Victoria La'bi, Obertus Bilang Laba, Muhammad Sabir Lalu Ahmad Zaenuri, Lalu Ahmad Lily Surayya Eka Putri, Lily Surayya Eka M Azhar Irwansyah, M Azhar Maivira, Enjelia Maryami, Maryami Maulana, Khaidir Muhammad Ichsan Munanjar, Azwar Mutia Dwi Haryanti, Mutia Dwi Mutiara, Rahma Nita Wijayanti, Ni Putu Noprisson, Handrie Nurdin Nurdin Nurdin, Muh. Nur Hidayat Nurul Fitroh, Nurul Oscar Yanuarianto, Oscar Ovra, Ovra Pribadi, Muhammad Fachrul Risqi Putra, Ganesh Rahmat Hidayat Ramlah Ramlah Resnani, Resnani Resnani, Resnani Rodiah Rodiah Rohmatin, Fitriyah Saifuddin Saifuddin Samsul Hadi Sanjaya, Ari Susandy Sarjito Sarjito Septian, Rio Silvia Silvia Srivani, Musbatik Subekti, Mohammad Sudarso, J Susi Susanti Suwandi Ng Triwani Triwani Uddin, Faqih Burhan vierto, vierto Weriantoni, Weriantoni Wiratama, Richard Wuisan, Felicia Yanti, Marince Fitri Yuliantini, Nani Yusuf, Syahril Zainur, Zainur Zulfiar, Edy