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Journal : ANIMAL PRODUCTION

Additional Freeze Drying Fig Fruit (Ficus Carica L) Filtrate into Tris Egg Yolk Extender and Its Effect on Sperm Membrane Integrity and Acrosome of Kacang Buck Zaenuri, Lalu Ahmad; Lukman, Lukman; Yanuarianto, Oscar; Sumadiasa, I Wayan Lanus; Rodiah, Rodiah
ANIMAL PRODUCTION Vol 19, No 3 (2017)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2017.19.3.647

Abstract

A study was designed to determine Kacang buck sperm membrane integrity and acrosome reaction as to the effect of different concentration of freeze drying fig fruit filtrate in tris egg yolk based extender. This study used 5 proven fertility Kacang goats aged 3-4 years, maintained by Faculty of Animal Sciences, Mataram University, Indonesia. Semen was collected by artificial vagina at every five days. The collected semen was divided into four aliquots in accordance to the treatments extender such as Control (0 gr), T1 (0.02 gr), T2 (0.04 gr) and T3 (0.06 gr) freeze drying fig fruit filtrate in tris egg yolk based extender (gr/v), respectively. Plasma membrane integrity and intact acrosome after re-concentration and preserved at 5ºC were assessed visually at 0 and every 24 hours for 5 consecutive days. The statistical significance of the result was evaluated by a one way ANOVA for completely randomized design analysis of variance. Data were presented as Mean±SD. Results suggest that the mean percentages of sperm membrane integrity in T0, T1,T2 and T3 at 96 h post extended and preserve at 5ºC were 34.3±5.3,  40.6±4.7,  44.8±5.4 and  42.1±5,1, respectively. The mean percentages of sperm acrosome intact were 16.4±4.8, 18.5±1.9,  21.6±3.1 and 19.6±2.8, respectively. The results of the study suggested that additional 0.04gr freeze drying fig fruit filtrate into tris egg yolk based extender have a significant preservation effect on both spermatozoa membrane integrity and acrosome intact of kacang buck.
Co-Authors Achmad Haikal, Achmad Achmad Supriyanto Agus Dwi Anggono Amsal Amri Andi Alimuddin Unde, Andi Alimuddin Anto Tri Susilo, Andri Anzalena, Ria Aptensi, Fenny Ardian, Ani Ardian, Ani Ari Susandy Sanjaya, Ari Susandy Arif Wijaya Arskadius A, Mohd. Asbah, Asbah Aswin Fitriansyah, Aswin Burhanuddin Burhanuddin Casanova, Gusti Chrismadha, T Dadi, Sri Deden Ibnu Aqil, Deden Ibnu Dila, Ratna Djuwita, Puspa Dwi Anggraini Fadma, Mike Wahyu Fakhrudin, Muhammad Fibriani, Fini Fitriani agustina Fransiskus Randa Giri Sucahyo, Yudho Gupron, Ahmad Hamidah Hamidah Harry Dhika, Harry Hartono Hartono Helfi Nasution Herawati Jaya Herman Yasin Hidayati Hidayati Hijril, Hijril Hujair A.H. sanaky Hustarna Hustarna, Hustarna I Wayan Lanus Sumadiasa, I Wayan Lanus Ilham Ilham Imam Sunoto, Imam Indra Sensuse, Dana Indrawan, Donny Ishartiwi Ishartiwi Joko Marwoto Karjiyati, Victoria La'bi, Obertus Bilang Laba, Muhammad Sabir Lalu Ahmad Zaenuri, Lalu Ahmad Lily Surayya Eka Putri, Lily Surayya Eka M Azhar Irwansyah, M Azhar Maivira, Enjelia Maryami, Maryami Maulana, Khaidir Muhammad Ichsan Munanjar, Azwar Mutia Dwi Haryanti, Mutia Dwi Mutiara, Rahma Nita Wijayanti, Ni Putu Noprisson, Handrie Nurdin Nurdin Nurdin, Muh. Nur Hidayat Nurul Fitroh, Nurul Oscar Yanuarianto, Oscar Ovra, Ovra Pambudi, Hushyain Pribadi, Muhammad Fachrul Risqi Putra, Arik Andrian Putra, Ganesh Rahmat Hidayat Ramlah Ramlah Resnani, Resnani Resnani, Resnani Rodiah Rodiah Rohmatin, Fitriyah Saifuddin Saifuddin Samsul Hadi Sanjaya, Ari Susandy Saputra, Dian Noviardi Saputra, Evriyana Indra Sarjito Sarjito Septian, Rio Silvia Silvia Slameto, Andika Agus Srivani, Musbatik Subekti, Mohammad Sudarso, J Susi Susanti Suwandi Ng Triwani Triwani Uddin, Faqih Burhan vierto, vierto Weriantoni, Weriantoni Wiratama, Richard Wuisan, Felicia Yanti, Marince Fitri Yuliantini, Nani Yusuf, Syahril Zainur, Zainur Zulfiar, Edy