Denny Widaya Lukman
Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner FKH IPB

Published : 18 Documents
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Detection of Mycobacterium avium subspecies paratuberculosis Formula Milk in Bogor

Jurnal Teknologi Dan Industri Pangan Vol 19, No 1 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which is usually associated to Crohn’s disease (CD) in human. The disease shows similarities in clinical signs and pathology characteristic with John’s disease (JD) in ruminants which is infected by MAP. Researchers in Europe, USA, and Australia detected MAP in their dairy products and showed the relationship among MAP, CD, and JD. Meanwhile Indonesia imported milk and milk products from those countries to cover the national demand. This situation keeps MAP as potential-problem in national dairy herd and human health in the future. The aim of this study was to detect MAP in the formula milk for toddler. Fifty samples from five established milk producers were taken on August 2006 at the supermarket in Bogor. Two separate diagnostic methods were used parallel in this study i.e polymerase chain reaction method (PCR) with insertion sequence F 57 as the primer and the Mycobacterial Growth Indicator Tube (MGIT). Neither MAP grew in MGIT after 20 weeks of incubation period but 5 samples were found positive by nested PCR. Although there was no evidence weather MAP grew from the samples, as well as in human to provide data on MAP in Indonesia. Key words: Mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR F57

Detection of Mycobacterium avium subspecies paratuberculosis in Growing up Milk Formula in Bogor

Forum Pasca Sarjana Vol 32, No 2 (2009): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Abstract

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which associated with Crohn’s disease (CD) in human and Johne’s disease (JD) in ruminants.  Some researchers in Europe, USA, and Australia detected MAP in the dairy products and showed the relationship among MAP, CD, and JD.  Meanwhile Indonesia imported milk and milk products from those countries to cover national demand.  In the future it will be a potential problem to national dairy herd and human health.  The aim of this study is to detect MAP in the growing up milk formula.  Fifty samples from five established distributors were taken in Bogor.  Some diagnostic methods were used parallel in this study, namely Mycobacterial Growth Indicator Tube (MGIT), Herrold’s Egg Yolk enrichment with mycobactine-J (HEYM) and polymerase chain reaction method (PCR) with insertion sequence IS 900 and F 57 as primer.  Neither MAP grew up in MGIT and HEYM after 20 weeks of incubation period. No positive samples were found by conventional PCR using IS 900 and F57 either but 5 samples were detected positive by nested PCR F57.  Although there was no evidence of MAP grew from the samples in this study, the comprehensive and sustainable studies on MAP still should be carried out with more and varied samples, as well as in human to provide data on MAP and to anticipate it in Indonesia.   Key words: mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR

Faktor Risiko Babi yang Diumbar dan Pakan Mentah Mempertinggi Prevalensi Sistiserkosis (RISK FACTOR OF FEE-RANGE HUSBANDRY AND RAW PORK FEED INCEASE THE PREVALENCE OF CYSTICERCOSIS)

Jurnal Veteriner Vol 13, No 4 (2012)
Publisher : Jurnal Veteriner

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Abstract

The aims of this study were to determine the prevalence and risk factors of porcine cysticercosis. Thesurvey was carried out in eight districts of Jaya Wijaya, Papua and Jibama market, during October 2009to June 2011. A total of 111 pigs were tested serologically. Serum samples were tested for the presence ofcirculating parasite antigen using monoclonal antibody-based sandwich enzyme-linked immunosorbentassay (MoAb-ELISA). Forty five samples (40.54%) were found positive by MoAb-ELISA and the highestprevalence occurred from the District of Asolokobal (92.86%), followed by Musatfak (75%), Kurulu (65.22%),Bolakme (33.33%), Asologaima (31.82%), Hom-hom (18.18%), Hubikosi (14.29%), Jibama trade (14.29%),and the lowest prevalence from Wamena Kota is 5.88%. Free-range pig husbandry system (OR=4.63;P<0.01) and uncook pork feed (OR=3.65; P<0.05) were important risk factors for porcine cysticercosis. It istherefore necessary to anthropology approach about pig husbandry system and pattern of cook pork feed.

Kemampuan Primer IS900 Dan F57 Mendeteksi Mycobacterium avium Subspesies Paratuberculosis Dengan PCR Konvensional

Jurnal Sain Veteriner Vol 26, No 1 (2008)
Publisher : Fakultas Kedokteran Hewan

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Abstract

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Pengembangan Enzyme-Linked Immunosorbent Assay Paratuberkulosis dengan Antigen Protoplasmik Mycobacterium avium Subspecies Paratuberculosis Isolat Lapang (DEVELOPMENT OF PARATUBERCULOSIS ENZYME-LINKED IMMUNO-SORBENT ASSAY WITH PROTOPLASMIC ANTIGEN OF MYCO

Jurnal Veteriner Vol 16, No 2 (2015)
Publisher : Jurnal Veteriner

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Abstract

Enzyme-linked immunosorbent assay (ELISA) is a serological test method most widely used for thediagnosis of paratuberculosis, because it has a better sensitivity compared to other serological test.Protoplasmic antigen (PPA) or cellular extract is still the main choice for the diagnosis of paratuberkulosisdevelopment. The aim of research was to use the PPA Mycobacterium avium subspeciesparatuberculosis(MAP) field isolates for the development of paratuberculosis ELISA (ELISA PPA-L). As many as 322cattle sera (300 negative and 22 positive) were tested using this method and compared with IDEXXcommercial kit. The sensitivity and specificity of ELISA PPA-L test results were 68.18% and 97.0%,whereas for the IDEXX kit were 63.64% and 97.33%respectively. ELISA PPA-L had higher sensitivity andlower specificity compared to the IDEXX commercial kit. ELISA test using protoplasmic antigen of MAPfield isolates has good ability for paratuberculosis serological test and can be used for screening test of thedisease in Indonesia.

Seroprevalensi Positif Sistiserkosis pada Babi Hutan di Kabupaten Way Kanan, Provinsi Lampung (POSITIVE SEROPREVALENCE OF WILD BOAR CYSTICERCOSIS IN WAY KANAN DISTRICT, LAMPUNG PROVINCE)

Jurnal Veteriner Vol 16, No 2 (2015)
Publisher : Jurnal Veteriner

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Abstract

Wild boar is also an intermediate host of Taenia solium, besides domestic pig which is the mainsource of Cysticercus infection in human. Cysticercosis in wild boar has not been reported in Indonesia.The aim of this study was to determine wild boar’s cysticercosis in Way Kanan District. One hundred serasamples consisting of 41 male wild boars and 59 female wild boars were collected. Eighty seven of boarscome from Banjit Subdistrict, while 13 of wild boars come from Blambangan Umpu Subdistrict in WayKanan District. The serum were tested serologically to determine antigen of parasite cycles. The test usedmonoclonal antibody-base sandwich Enzyme-Linked Immunosorbent Assay (Moab-ELISA). There is asero positive reaction of cysticercosis(1%) which is female wild boar from Banjit sub district. Controllingof meat inspection should be conducted to prevent cysticercosis transmission to human.

SeroprevalensiTrichinellosispadaBabi di Kota Kupang, Provinsi Nusa Tenggara Timur (SEROPREVALENCE OF TRICHINELLOSIS ON PIG AT KUPANG CITY, PROVINCE OF NUSA TENGGARA TIMUR)

Jurnal Veteriner Vol 16, No 3 (2015)
Publisher : Jurnal Veteriner

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Abstract

Trichinellosisis is a zoonotic disease caused by infection of Trichinella spp. and is found world-wide.The seroprevalence of trichinellosis in pig population has never been reported in East Nusa TenggaraProvince particularly Kupang City. The aim of this study was to determine the seroprevalence oftrichinellosis in pig at Kupang City, East Nusa Tenggara. A cross-sectional study was conducted byexamining 376 blood samples of pigs collected from slaughterhouses in Oeba, Kupang using simplerandom sampling method. Blood samples were tested by indirect enzyme linked immunosorbent assay(ELISA) using Screen®Trichinella indirect multi-species ELISA kit. Test results showed that 3 (0.8%)serum samples were positive of trichinella antibody. In conclusion ,some pigs in the city of Kupang hasbeen infected by Trichinella sp. and could be a threat for human health.

Deteksi Penyakit Bovine Viral Diarrhea pada Sapi Potong Impor melalui Pelabuhan Tanjung Priok

P-ISSN 2337-3202
Publisher : Bogor Agricultural University

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Abstract

Penelitian ini bertujuan untuk melakukan suatu kajian serologis tentang penyakit bovine viral diarrhea (BVD) dan mendeteksi adanya kaitan pemeliharaan kandang sebagai faktor risiko sumber penularan penyakit BVD pada sapi potong impor. Pengujian screening awal mengggunakan ELISA (enzyme linked immunosorbant assay) Antibodi BVD terhadap 100 sampel serum darah sapi, dan ditemukan 63 positif terhadap adanya antibodi anti BVD. Selanjutnya dilakukan pengujian lanjutan ELISA Antigen BVD dan hasilnya seluruh sampel negatif terhadap Antigen BVD. Hasil positif uji ELISA terhadap antibodi BVD mengindikasikan bahwa sampel mengandung antibodi anti BVD akibat pernah terinfeksi oleh virus BVD secara sementara (transient) atau melalui vaksinasi. Berdasarkan dokumen health certificate dari negara asal tidak terdapat informasi yang jelas terhadap perlakuan vaksinasi BVD pada sapi potong impor. Oleh karena itu diperlukan pemeriksaan screening di negara Indonesia untuk mendeteksi dan melakukan usaha preventif mencegah penyebaran di feedlot. Faktor-Faktor yang mempengaruhi kejadian hasil ELISA antibodi positif BVD, terkait dengan penyebaran penyakit BVD selama dalam masa pemeliharaan dan penggemukkan adalah program biosekuriti pada peternakan dengan nilai (OR=3,316; CI=1,380-7,967), dan pengelolaan limbah kandang dalam peternakan dengan nilai (OR=2,667; CI=1,105-6,434). Hasil ini menunjukkan ada asosiasi antara kedua faktor yang ada pada peternakan dengan kejadian penyakit BVD.Kata kunci: BVD, ELISA antibodi dan antigen, faktor risiko. (Detection and Risk Factors Study of Bovine Viral Diarrhea in Cattle Imports at Tanjung Priok Port)This research was a serological study on bovine viral diarrhea (BVD) and also to detect the relevance of maintenance farm management as a risk factor on the spreads of BVD. The initial screening test was performed using antibody capture enzyme linked immunosorbent assay (ELISA) to BVD on 100 cattle blood serum samples. The screening test showed that of 63 samples were positive to BVD antibody and 37 samples were negative to BVD antibody. The next screening test was performed using antigen capture ELISA to BVD and all samples showed negative results on BVD antigen. The results of the ELISA test positive for antibodies to BVD indicates that the samples examined anti-BVD antibodies due to BVD virus had been infected by a temporary (transient) or vaccination. Based on the document health certificate from the country of origin there is no clear information on the treatment of BVD vaccination on imports of beef cattle. So, we need a screening examination in the country of Indonesia to detect and perform preventive measures to prevent the spread in feedlots. Relevant factors that affected the occurrence of positive result on BVD antibody detection was farm biosecurity programs with odds ratio (OR) value of 3.316 and confidential interval (CI) value of 1.380-7.967. Further relevant factor was caging waste management with OR value of 2.667 and CI value of 1.105-6.434. There were statistically significant differences (p<0.05) between farm biosecurity programs and caging waste management related to BVD disease incidence.Keywords: BVD, ELISA antibodies and antigen, risk factors

Keberadaan Bakteri Listeria monocytogenes pada Keju Gouda Produksi Lokal dan Impor (PRESENCE OF LISTERIA MONOCYTOGENES IN LOCAL AND IMPORTED GOUDA CHEESES)

Jurnal Veteriner Vol 15, No 2 (2014)
Publisher : Jurnal Veteriner

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Abstract

Listeria monocytogenes is included in the foodborne pathogen, which has been associated with severaloutbreaks of human listeriosis especially in high risk groups. Listeria monocytogenes could be found inGouda cheeses because of poor hygienic and sanitation practices. In addition, this bacteria could surviveduring the making of cheese and cheese ripening process. The purpose of this study was to identify thepresence of L. monocytogenes in local and imported Gouda cheeses and how the safety level of the Goudacheese against contamination of L. monocytogenes. This study used the conventional method in accordancewith the Bacteriological Analytical Manual, US Food and Drug Administration and Bergey’s Manual ofDeterminative Bacteriology to detect the presence of L. monocytogenes at 15 samples of local Gouda cheeseand 15 samples of imported Gouda cheese sold in supermarkets in Jakarta and Bogor. The results of thisstudy showed that was not found L. monocytogenes in local and imported Gouda cheese. It could be concludedthat is Gouda cheese relatively safe from L. monocytogenes and meets Indonesian National Standard.

Cemaran Escherichia coli pada Tepung Telur yang Diimpor Melalui Pelabuhan Tanjung Priok, dan Resistensinya Terhadap Antibiotik (ESCHERICHIA COLI CONTAMINATION ON EGG POWDER IMPORTED THROUGH PORT OF TANJUNG PRIOK AND ITS RESISTANCE AGAINST ANTIBIOTICS)

Jurnal Veteriner Vol 17, No 2 (2016)
Publisher : Jurnal Veteriner

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Abstract

Eschericia coli contamination was found on egg powder imported to Indonesia during microbialexamination. The study was conducted to describe the presence of E. coli and its resistance to antibioticsin egg powder imported to Indonesia through The port of Tanjung Priok. Samples was determined usingcross sectional study and the sample size was calculated based on assumption of confidence level of 95%with margin of error of 10% and predicted prevalence of 50%. Total of 100 egg powder samples wascollected in August 2014 from two exporting countries, Ukraine (whole egg powder, n=30) and India (wholeegg powder, n=40 and egg yolk powder, n=30). Examination was performed by packaging and label inspectionof the product followed by samples collection and testing for bacterial contamination using rapid test andconventional isolation and identification methods. Detected E. coli was isolated and then tested forantibiotic resistance. Examination results showed that 4 samples were positive to E. coli. E. coli isolatesshowed resistance against 6 types of antibiotics and 75% of the isolates had resistance against minimumof 3 types of antibiotics. These conditions should be taken into consideration since antibiotic resistance inE. coli would cause negative impacts on human, animal and environmental health.