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Pelacakan Gen Aerolysin dari Aeromonas hydrophila pada Ikan Mas yang Diberi Pakan Ekstrak Bawang Putih (DETECTION OF AEROLYSIN GEN FROM AEROMONAS HYDROPHILA IN COMMON CARP FED WITH GARLIC EXTRACT) Lukistyowati, Iesje; ., Kurniasih
Jurnal Veteriner Vol 13, No 1 (2012)
Publisher : Jurnal Veteriner

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Abstract

Aeromonas hydrophila is a gram negative and opportunistic bacteria, which could cause fish mortalityin a short time from 80%-100%. One virulent factor of A. hydrophila on common carp (Cyprinus carpio L)that could cause fish mortality is aerolysin. This research used a synthetic primers of oligonukleotide todetect aerolysin, a specific genomes of A. hydrophila on common carp (Cyprinus carpio L). The commoncarps have been feed a woof that contain garlic extract during 30 days before they challenged with A.hydrophila. Polymerase Chain Reaction (PCR) was used to detect an aerolysin gen from A. hydrophila. Theelectrophoresis result showed aerolysin gene of Aeromonas hydrophila from Veterinary Faculty of GadjahMada University (FKH-UGM) isolate was amplified with 462 bp of molecule weight. While the aerolysingen was detected in the fish kidney with 900 bp of molecule weight. Further, DNA sequence analysis of thePCR product of A. hydrophila from FKH – UGM isolate showed homolog with isolate A. hydrophila subsphydrophila ATCC 7966 complete genome with score 55.4 (71%).
KELANGSUNGAN HIDUP IKAN MAS (Cyprinus carpio L) YANG DIBERI PAKAN EKSTRAK BAWANG PUTIH (Allium sativum) DAN DI INFEKSI Aeromonas hydrophila Lukistyowati, Iesje
Jurnal Perikanan Dan kelautan Vol 16, No 02 (2011)
Publisher : Jurnal Perikanan Dan kelautan

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The aim of a research were to investigate the effectiveness of garlic extractmixed with feed given 30 days in encharging common carps after challen with againstAeromonas hydrophila with density of 106 cell/ml. This research conducted by usingcomplete random design with dose addition of garlic extract to feed were controlpositive, control negative, P1 (2,5%), P2 (5%), and P3 (10%). In order to fine out theactive component of garlic extraction methode was adopted using 70% ethanol. Theextracted filtrates were separated using the spectrophotometrical chromatrographymethod (GC-MS) and thin-layered chromatography. Identification of the maincomponents found in the ethanol extract of garlic revealed 13 components but the onethat served as antimicrobial was C6H10S2 CAS disulphide di 2-propenyl(CAS)/(Diallyldisulfide) with and area of 16,95%. Beside the result of thin layeredchromatography showed that garlic extact contained sapononin which is harmful toaquatic animals. Percentage of survival rate P3 (10%) after infected after withAeromonas hydrophila was different compare witn control positive. The highsurvival shown on P3 (10%) concentration with 70%, P1 60%, control negative 60%and P2 30%. Fourteen days after injection the cut cause by Aeromonas hydrophilaclose and the fish recuperated as shown by their lively movement.
PENGGUNAAN EKSTRAK BAWANG PUTIH UNTUIC MENCEGAH SERANGAN PENYAKIT MAS PADA IKAN MAS (Cyprinus carpio) Riauwaty, Morina; Lukistyowati, Iesje
Jurnal Terubuk Vol 35, No 01 (2007)
Publisher : Jurnal Terubuk

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Abstract

A study aims to understand the effectiveness of garlic extract in preventing the MASdisease that is caused by Aeromonas hydrophyla has beeN conducted in September - October2005. Fingerlings of common carp (8 - 12 cm TL) were immersed in 0,1000, 2000 and 3000ppm of garlic extract (10 minutes, 4 times, 4 days interval). Ten days after the last immersion,fishes were infected with A. hydrophyla (intra peritoneal injection). Survival rate and clinicalsigns of MAS diseases were monitored everyday for 7 days. Results shown that fishes Thatwere treated with 2000 and 3000-ppm of garlic extract do not show any clinical signs ofMAS disease and their survival rate (100%) were higher than that of the 1000 ppm andcontrol (83,33% and 61,11% respectively).
TELAAH KUALITAS AIR DENGAN TEKNOLOGI BERBEDA DAN ANALISIS BAKTERI PATOGEN (Aeromonas salmonicida )PADA IKAN PATIN DI KECAMATAN BANGKINANG BARAT KABUPATEN KAMPAR PROVINSI RIAU Nugraha, Fabri Putra; Siregar, Yusni Ikhwan; Lukistyowati, Iesje
Jurnal Ilmu Lingkungan Vol 8, No 1 (2014)
Publisher : Jurnal Ilmu Lingkungan

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This study was conducted from October to December 2012 at the subdistrict of WestBangkinang, Riau Province. The study aimed to described the basic information ofwater quality in different technology of maintenance (traditional, semi intensive andintensive) catfish in freshwater pond especially the contains of macrozoobenthic. Watersample were taken from three station whereas catfish sample were taken in threelocation and each station consist of 4 heads in each station. The catfish sample will betaken every week for 1 month. The results of (H’) and (C), station 3 had low (H’) withdominanted of (C), and it means they got hard pressure of environment activity.Besides, Aeromonas caviae was found in all technology of maintenance freshwaterponds. The result of Aeromonas salmonicida bacteria, was founded in three sample incatfish of different freshwater pond as A. sub smithia and A. sub achromogenes. Inaddition to measurement of water quality consist of temperature 27,9-28,6 0 C; pH : 5,7-6,4; DO :4,9-5,7 ppm; BOD :11,3-14,7ppm; COD : 14,7-18,2 ppm. Physics andchemistry sediment is muddy more than 65% from each station. According toPP.82/2001 about water quality of aquaculture, it was putted on the 3 rd class.Keywords: Macrozoobenthic, water quality, aeromonas salmonicida
Differentiation Of Leukocytes Cat Fish (Mystus Nemurus) Fed Solutions Containing Curcuma (Curcuma Xanthorrhiza) Siagian, Juandi Riki Umbara; Lukistyowati, Iesje; Riauwaty, Morina
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 1, No 2 (2014): Wisuda Oktober Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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The effect of containing curcuma (Curcuma xanthorrhiza Roxb) on diferentiation of leukocytes of Cat Fish (Mytus nemurus). The aim of the study is to find of diferentiation of leukocyte of Cat Fish (Mytus nemurus). The study was conducted from March to April 2014 in the Experimental Pond and Laboratory of Parasitic Diseases of Fish and Fisheries and Marine Sciences Faculty of the Riau University. The method used was experimental method using RAL with one factor with 4 level treatments and three repliations. the result indicate that the best differentiation of leukocytes in the P3 treatment is total leukocytes (96.538 cell/mm3), diferentiation of leukocytes (lymphocytes: 96,67%, monocytes: 1,67%, and neutrophils: 1,67%) and the best treament of survival rate is P2 and P3 with survival rate is 100%.Keywords: Differentiation, Leukocytes, Cat Fish, and Curcuma Solution
HISTOPATHOLOGY OF Mystus nemurus LIVER THAT WERE FED WITH FEED ADDED WITH Psidium guajava AND Andrographis paniculata NESS S, Morina Riauwaty; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was conducted on June to August 2014 in the Laboratory of Parasitesand Diseases of Fisheris and Marine Science of Faculty Riau University Pekanbaru. Thepurpose of this research was to determine the structure of (Mystus nemurus) liver that werefed with feed added with Psidium guajava and Andrographis paniculata Ness. The methodused is an experimental using 3 treatments with 3 replicated. The treatment used in thisresearch was Kn (Fishes were not receive any treantent), Kp (Fishes were not receive anytreatment but were infected with A. hydrophila), P1 ( Fishes were fed with feed added with10 g of Psidium guajava and 10 g of Andrographis paniculata Ness and were infected withA. hydrophila (0.1 ml of 108 of A. hydrophila culture), P2 (Fishes were fed with feed addedwith 20 g of Psidium guajava and 20 g of Andrographis paniculata Ness and were infectedwith A. hydrophila (0.1 ml of 108 of A. hydrophila culture), P3 (Fishes were fed with feedadded with 30 g of Psidium guajava and 30 g of Andrographis paniculata Ness and wereinfected with A. hydrophila (0.1 ml of 108 of A. hydrophila culture). Fish organ (liver) wereprocessed for histological studied (formalin fixed, alcohol series, 6 sliced and HE Stained).Result indicate that the structure of liver of the treated fish showing abnormality symptoms,such as necrosis, fatted degeneration and hemorrhage. It can be concluded that feeding offish with 30 g of Psidium guajava and 30 g of Andrographis paniculata Ness is able to treatA. hydrophila infection on Mystus nemurus.Key word : Liver, Mystus nemurus, Psidium guajava, Andrographis paniculata Ness.
THE EFFECT OF THE ADDITION OF GINGER (Curcuma xanthorrhiza ROXB) TO FEED TO TOTAL ERYTHROCYTES, HEMATOCRIT, HEMOGLOBIN and GROWTH OF CATFISH (Mystus nemurus) Yulistia, Feri; Lukistyowati, Iesje; Riauwaty, Morina
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was done in March - April 2014 in Fish Disease Parasite and Laboratories and an Experiment of the Faculty of Fisheries and Marine Science University of Riau. The purpose of this research is to know the influence of the addition of curcuma on feed to the total of erythrocytes , hematocrit , hemoglobin and the growth of catfish. A research method used is the design of random complete with one factor which feed containing curcuma with four standard treatment consists of , P0: control without given ) curcuma solution , the P1 ( curcuma solution with a dose of 2 g/kilogram feed ) , the P2 ( curcuma solution with a dose of 4 g/kilogram feed ) , the P3 ( curcuma solution with a dose of 6 g/kilogram feed ). The results of research shows that the addition of a solution on feed curcuma doesn’t impact the condition of fish blood because of poor environmental conditions. It can increase the growth of absolute weight catfish .The total erythrocytes is ranged between 1,25-1,91 mm3/ million cells , hematocrit 13,8-18,5 % and the percentage of hemoglobin 3,9-4,2 g/dL.Keywords: River catfish (Mystus nemurus), ginger, blood catfish
SENSITIVITAS EKSTRAK BUAH MENGKUDU (Morinda citrifolia L.) TERHADAP BAKTERI Streptococcus agalactiae Muharram, Anzila Rizki W; Syawal, Henni; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTThe research was conducted from October to December 2014 in the Laboratory of Parasitic Diseases of Fish Faculty of Fisheries and Marine Sciences, and Laboratory of organic and natural material of Faculty of Mathematics and Natural Sciences, University of Riau, Pekanbaru. The purpose of this study to determine the concentration of noni fruit (Morinda citrifolia L.) that can inhibit the growth of Streptococcus agalactiae and safe dose to be given to the fish. The method that had been used was experimental method with concentration : P0: control, P1 (4%), P2 (5%), P3 (6%), P4 (7%), P5 (8%), P6 (9%), P7 (10%). The results show noni fruit solution to Streptococcus agalactiae sensitive average highest inhibition zone formed on treatment P7 (10%) of 9.83 mm and significantly different between treatments (P <0.05). MIC test of extract methanol Morinda citrifolia L. on treatment P2 (5%) of 78 x 108 cfu/mL. LD50 test solution for tilapia (Oreochromis niloticus) extract methanol Morinda citrifolia L. marinated for 24 hours after Reed and Muench analysis contained in the concentration of 1.42%.Key word: Morinda citrifolia L., Streptococcus agalactiae, Sensitivitas, MIC, LD50.
Sensitivity of Mangosteen Rind (Garcinia mangostana L.) Solution Toward Aeromonas hydrophila M, Dian Fitria; Lukistyowati, Iesje; Syawal, Henni
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTResearch was conducted from September to October 2014, at the labory Examination of Fish Disease, Fisheries and Marine Sciences Faculty of Riau University. The Purpose of this study was to determine the sensitivity of mangosteen rind (G. mangostana L.) as antimicrobial to A. hydrophila, the range of Minimal Inhibitory Concentration (MIC) and the lethal dose (LD50) mangosteen rind (G. mangostana L.) solution against to catfish (Clarias gariepinus) (10-13 cm) by immersion. The research method used doses 10 % (19 g/L), 20 % (38 g/L), 30 % (57 g/L), 40 % (76 g/L), 50 % (95 g/L), 60 % (114 g/L), 70 % (133 g/L), 80 % (152 g/L), 90 % (171g/L), 100 % (190 g/L). The results showed that mangosteen rind was effectively to inhibit the growth of bacterial A. hydrophila at a dose of 50 % (95 g/L) inhibitory zone of 13,65 mm, with the number of A. hydrophila colonies in TSA medium dose 50 % (95 g/L) is 30,6 x 108 cfu/mL. LD50 test was obtained in the dose mangosteen rind 4, 43 g/L.Key words : Garcinia mangostana L., Aeromonas hydrophila, antimicrobial, lethal dose
PEMBERIAN PROBIOTIK Bacillus sp BERASAL DARI UDANG GALAH (Macrobranchium rosenbergii DE MAN) DAN UDANG WINDU (Penaeus monodon) TERHADAP STATUS KESEHATAN IKAN NILA(Oreochormis niloticus) Lukistyowati, Iesje; Syawal, Henni
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 1 (2017): Wisuda Februari Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was conducted on October 2015 - June 2016, at the Laboratory of Microbiology and the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine Sciences, University of Riau. The purpose of this study is to comprehend the probiotic Bacillus sp. from the digestive tract of the giant prawns (Macrobranchium rosenbergii DE MAN) and the black tiger shrimp (Panaeus monodon) for the health status of tilapia (Oreochormis niloticus). This study uses a Completely Randomized Design (CRD), which consists of three treatments and each of them uses three replications for the probiotic Bacillus sp. treatment of the digestive tract either from the giant prawns or the tiger shrimp. The Bacillus sp. treatment from the giant prawns are P1G (UG4 isolates), P2G isolates combined (UG1, UG2, UG3, UG4, and UG5) and as a control (P0).While the tiger shrimp are using P1W (UWH9 isolates), P2W isolates that combined (UWH1, UWH2, UWH8, UWH9 and UWH 10) and as a control (P0). Probiotics from each treatment were put into the medium water about 3 mL / container with a density of 104 CFU/ml. The results showed that the P1W treatment is the best treatment after infected by Streptococcus iniae for the health statusof tilapia, it is showed by the number of Erythrocytes 246.67x104 sel / mm, Haemoglobin level of 6633 g/dl, Hematocrytes 30.33%, and total Leukocytes 79,33x103 sel/mm3. Given by probiotics of Bacillus sp that originatit from the digestive tract of giant prawns and tiger shrimp through the water were able to maintain the health of tilapia (Oreochormis niloticus).Key Words: Bacillus sp, giant prawns, black tiger shrimp, health status, tilapia