Triva Murtina Lubis
Department of Physiology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh

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MEASUREMENT OF SERUM TESTOSTERONE IN KACANG GOAT BYUSING ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TECHNIQUE: THE IMPORTANCE OF KIT VALIDATION (Pengukuran Testosteron Serum Kambing Kacang dengan Teknik Enzyme-Linked Immunosorbent Assay (ELISA): Pentingnya Validasi Kit)

Jurnal Kedokteran Hewan Vol 10, No 1 (2016): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

This study was conducted to validate a commercial testosterone enzyme-linked immunosorbent assay (ELISA) kits (DRG EIA-1559) inanalytic and biological manner for measuring serum testosterone concentrations in kacang goats. This study used 18 healthy kacang goats, six bucks (>2 years), six kids (<6 months), and six does (>2 years). Blood samples were collected from jugular vein and prepared as serum. Two validation tests were performed, an analytical validation comprises a parallelism, accuracy, precision and sensitivity and a biological validation by comparing testosterone concentration from bucks, kids, and does. Testosterone concentrations were measured using ELISA technique. Data of analytical validation were analyzed descriptively and test of equality of slope was performed to see the parallelism between samples and standard curves. Analysis of variance (ANOVA) was used for biological validation data. Results of parallelism showed that sample curve was parallel to the standard curve. Accuracy, precision (% CV of intra-and inter-assay) and sensitivity of the assay were 99.65±4.27%, <10%, <15% and 0.083 ng/ml, respectively. Results of biological validation showed that the assay used were accurately measured testosterone which testosterone concentrations in bucks were significantly higher compared to kids and does (P<0.05). In conclusion, a commercial testosterone ELISA kits (DRG EIA-1559) is a reliable assay for measuring serum testosterone concentration in kacang goats. Key words: analytical and biological validations, ELISA, testosterone, kacang goat

The traits of fresh and frozen semen on brahman bulls

Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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The present study aimed to determine quality and parameters traits of both fresh and frozen semen in 4-year old Brahman bulls. Semen was collected from 5 bulls twice a week using artificial vagina in Ungaran ArtificialInsemination Center, Central Java, Indonesia. Immediately after collection, samples were evaluated for semen volume, color, odor, viscosity, mass movement, individual movement, spermatozoa concentration and livespermatozoa percentage. Sperm concentration was measured using a spectrophotometer. Good quality fresh semen was processed to be frozen semen. Parameters evaluated for frozen semen include spermatozoa individualmovement, live spermatozoa, and abnormal spermatozoa percentage. The results showed that the semen volume is 6.90 ± 0.54 whereas the average of sperm concentration, individual motility and live sperm are 1754.54 ± 212.67;65.50 ± 3.74% and 76.30 ± 2.64%, respectively. Meanwhile, for frozen semen, the average percentage of motility is 38.00 ± 2.74% while the average proportions of live sperm and abnormal sperm are respectively 45.20 ± 9.86% and 6.00 ± 2.83%. In conclusion, in average, all parameters of fresh and frozen semen of Brahman bulls are in normal range. Based on the Indonesian National Standard, frozen semen produced could be used for insemination

Hematology profile of Sumatran Orangutan (Pongo abelii) in the Sumatran Orangutan Quarantine Center, Sibolangit, Indonesia

Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

This research aims to obtain hematological database of healthy Sumatran orangutans (Pongo abelii) at the Sumatran Orangutan Quarantine Centre, in Batu Mbelin, Sibolangit, North Sumatra, Indonesia. This secondary datacomes from hematological examination results in 52 healthy Sumatran orangutans in Batu Mbelin, from May 2003 to August 2010. This research used a factorial complete randomized design (CRD) that has two factors namely, gender as the first factor and age as the second factor. Data was analyzed using analysis of variance (ANOVA). The results of ANOVA show that gender affects (P<0.05) on the value of mean cell hemoglobin concentration (MCHC) in Sumatran orangutans. Age influences (P<0.05) on the lymphocytes and monocytes Sumatran orangutans. The results of least significant different (LSD) test of the lymphocyte show that there are significant differences (P<0.05) between the lymphocyte value of infants and that of juveniles. The lymphocyte value of infant is also significantly different (P<0.05) that of adolescent. While the lymphocyte value of adolescent and juveniles are not significant different. The results of LSD test of the value of monocytes show that there is a very significant difference (P<0.01) between the monocyte value of infants and that of juveniles. Furthermore, the value of infant’s and adolescent’s monocytes issignificantly different (P<0.05). However, the value of adolescent’s and juvenile’s monocytes does not show any influences. Interaction of sex influences on the red blood cell (RBC) of Sumatran orangutans whereas age do notinfluences on the RBC. The results of Duncan test show that there are significant differences (P<0.05) between the RBC values of adolescent female orangutans and adolescent male orangutans. Other hematological values do not show any effects on both gender and age

Characteristics of frozen-thawed semen on Simmental and Limousin bulls in Ungaran, Indonesia

Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The present research aimed to study the characteristics of frozen-thawed semen in beef bulls ex-import in Ungaran, Indonesia. 5 heads Simmental and 5 heads Limousin of 5.5 years old were used in this research. The research was done in Ungaran Artificial Insemination Center, Central Java, Indonesia and Laboratory of Reproduction and Obstetry, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia. Frozen semen was obtained from this AI center. Results of the research indicated that the average individual motility of Simmental and Limousin is 39.00±5.48% and 36.00±2.24%. The average percentage of live spermatozoa is 54.00±10.75% for Simmental and 48.20±9.78% for Limousin while  the average proportion of abnormal spermatozoa is 9.60±3.36% and 6.80±4.15% for both Simmental and Limousin, respectively. On average, Simmental frozen-thawed semen have the higher mean proportion of sperm motility, live sperm, and abnormal sperm than that of Limousin frozen-thawed semen

Motilitas Spermatozoa Ayam Kampung dalam Pengencer Air Kelapa, NaCl Fisiologis dan Air Kelapa-NaCl Fisiologis pada 25-29°C

Jurnal Agripet Vol 11, No 2 (2011): Volume 11, No. 2, Oktober 2011
Publisher : Agricultural Faculty

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Abstract

Spermatozoa motility of local chicken in coconut water, physiological nacl and physiological nacl-coconut water at 25-29°c)ABSTRACT. This study aimed to determine the effect of coconut water diluent, physiological NaCl and combination of coconut water-physiological NaCl to the survival of chicken spermatozoa at room temperature (25-29°C). Semen was collected from a local chicken of 1.5 years old with weight of 2 kg in massage twice a week. Once evaluated, a good quality semen was divided into 3 treatment groups: coconut water (P1), physiological saline (P2), and its combination (P3) with the addition of 20% egg yolk [(P1) 80:0:20 (P2) 0:80:20 and (P3) 40:40:20] and stored at room temperature. Evaluation of spermatozoa motility conducted every 1 hour until reaching 40% of spermatozoa motility. Data obtained were analyzed using analysis of variance (ANOVA) with design patterns Split-plot and if there are differences between treatments tested by Duncan's multiple test. The results showed that motility of spermatozoa is significantly different (p <0.05) between storage time and diluent and there are interactions between storage time and diluent. P2 is able to maintain the minimum percentage of inseminated sperm motility (40%) for 4 hours, followed by P3 for 1 hour, and the lowest percentage motility of spermatozoa present in P1 for 0 hours. It can be concluded that the percentage of spermatozoa motility in group P2 is higher than P1 and P3.

19. PENGARUH PEMBERIAN EKSTRAK TEH HIJAU (Camelia sinensis) TERHADAP PENURUNAN KADAR HEMOGLOBIN DAN NILAI HEMATOKRIT PADA TIKUS WISTAR (Rattus norvegicus) The Effect of Green Tea Extract (Camelia sinensis) on Haemoglobin and Hematocrit Levels of Wistar Rat (Rattus norvegicus)

Jurnal Medika Veterinaria Vol 10, No 2 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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Abstract

The aim of this study was to determine the effect of green tea (Camelia sinensis) extract on hemoglobin and hematocrit level of Wistar rat (Rattus norvegicus). Sample animals used were 20 rats strain Wistar aged 2.5-3.0 months. Green tea extract was given for 30 days consecutively. Blood collection was conducted via orbitalis sinus for 2 ml using capillary pipette. Rats were fed with standart commercial food (Turbo Feed T.79-4) and aquadest as drinking water ad libitum. This study used completely randomized design (CRD) pattern with 4 treatments and 5 replications. The control group (P0) without any treatment, P1 was given green tea extract 0.045 g/ml/day, P2 was given green tea extract 0.060 g/ml/day, and P3 was given green tea extract 0.075 g/ml/day. Data were analyzed by oneway analysis of variance (Anova). The result showed that mean±SD of hemoglobin levels (g/dl) on each treatment group (P0, P1, P2, and P3) were 11.86±0.55, 12.58±1.40, 11.82±0.69, and 12.50±0.84 respectivey. Mean±SD of hematocrit levels (%) on each treatment group (P0, P1, P2, and P3) were 44.00±4.30, 43.40±1.14, 44.00±1.58, and 42.80±1.79. To conclude, the administration of green tea extract has no effect (P>0.05) on haemoglobin and hematocrit levels of Wistar rat. ____________________________________________________________________________________________________________________ Key words: green tea, haemoglobin, haematocrit, Wistar rat

PERSENTASE SPERMATOZOA HIDUP PADA TIKUS WISTAR DAN SPRAGUE-DAWLEY

Jurnal Medika Veterinaria Vol 7, No 2 (2013): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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Abstract

Penelitian ini bertujuan menentukan dan membandingkan persentase spermatozoa hidup dari kauda epididimis pada tikus strain Wistar dan Sprague-Dawley. Dua puluh ekor tikus jantan (Rattus norvegicus) yang terdiri dari 10 ekor strain Wistar dan 10 ekor strain Sprague-Dawley berumur 5 bulan digunakan dalam penelitian ini. Pada saat berumur 2,5 bulan seluruh tikus diadaptasikan selama 75 hari di dalam kandang dan diberikan pakan Turbo Feed T.79-4 (PT. Central Protein Prima, Medan) dan minuman secara ad libitum. Setelah masa adaptasi selesai, setiap hari diambil masing-masing dua ekor strain Wistar dan Sprague-Dawley untuk dibedah dan diambil kauda epididimisnya. Selanjutnya dibuat preparat hapus semen dengan menggunakan pewarnaan eosin untuk menghitung jumlah spermatozoa hidup. Preparat diamati di bawah mikroskop dengan 5 lapangan pandang. Data yang diperoleh dianalisis menggunakan uji T. Hasil penelitian menunjukkan bahwa terdapat perbedaan rata-rata persentase spermatozoa yang sangat nyata (P<0,01) antara strain tikus Wistar (92,00±2,83%) dan Sprague-Dawley (90,70±4,37%). Disimpulkan bahwa persentase spermatozoa hidup pada tikus Wistar lebih tinggi daripada tikus Sprague-Dawley

Determination of Cadmium Accumulation in Livers and Feces of Kacang Goats Grazing Tsunami Affected Land

Jurnal Agripet Vol 10, No 1 (2010): Volume 10, No. 1, April 2010
Publisher : Agricultural Faculty

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Abstract

Penentuan akumulasi cadmium (Cd) dalam hati kambing kacang di daerah tsunamiABSTRAK. Tujuan dari penelitian ini adalah untuk mendeteksi akumulasi cadmium pada hati dan feces kambing Kacang dan sekaligus mengukur kadar akumulasi cadmium tersebut. Limabelas ekor kambing Kacang yang berasal dari rumah potong di daerah Tsunami dan limbelas ekor kambing Kacang yang berasal dari rumah potong di daerah Non-Tsunami. Diikuti dengan lima ekor kambing Kacang dari desa di daerah Tsunami dan lima ekor kambing Kacang dari desa di daerah Non-Tsunami. Ke-empatpuluh ekor kambing Kacang tersebut disembelih untuk diambil hati dan feces. Sebelum hati dan feces tersebut akan dipreparasi untuk analisis akumulasi dari cadmium, dilakukan pengamatan anatomi dari hati dan feces tersebut. Cadmium berakumulasi dihati dan feces dari kambing Kacang, baik yang berasal dari daerah Tsunami dan dari daerah Non-Tsunami. Akumulasi cadmium didalam hati kambing Kacang signifikan lebih tinggi di daerah Non-Tsunami dibandingkan didalam hati kambing Kacang di daerah Tsunami. Pada feces kambing Kacang akumulasi cadmium juga signifikan lebih tinggi di daerah Non-Tsunami dibandingkan didalam feces kambing Kacang di daerah Tsunami.

MOTILITAS DAN VIABILITAS SPERMATOZOA SAPI ACEH SETELAH PEMBEKUAN MENGGUNAKAN PENGENCER SITRAT KUNING TELUR DENGAN PENAMBAHAN EKSTRAK KULIT BUAH NAGA MERAH (Hylocereus polyrhizus)(Motility and Viability Spermatozoa Aceh Cattle after Freezing Using Yolk Citrate Diluent with Additional of Peels Red Dragon Fruit Extract (Hylocereus polyrhizus))

JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 2 (2018): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

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Abstract

Penelitian ini bertujuan mengetahui pengaruh penambahan ekstrak kulit buah naga merah (Hylocereus polyrhizus) dalam pengencer sitrat kuning telur terhadap motilitas dan viabilitas spermatozoa sapi aceh setelah pembekuan. Penelitian ini menggunakan semen segar sapi aceh yang dikoleksi menggunakan vagina buatan dan dibagi ke dalam 5 kelompok perlakuan pengencer dengan 5 kali ulangan. Kelompok perlakuan kontrol yaitu semen diencerkan dalam pengencer sitrat kuning telur tanpa penambahan ekstrak kulit buah naga merah (P0), kelompok perlakuan semen diencerkan dalam pengencer sitrat kuning telur ditambah ekstrak kulit buah naga merah 0,2% (P1), 0,4% (P2), 0,6% (P3) dan 0,8% (P4). Data dianalisis menggunakan uji analysis of variance (ANOVA) satu arah dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa penambahan ekstrak kulit buah naga merah konsentrasi 0,0; 0,2; 0,4; 0,6 dan 0,8% dalam pengencer sitrat kuning telur menghasilkan persentase motilitas adalah 23,60 ± 4,72; 26,60 ± 5,94; 36,40 ± 4,98; 45,40 ± 4,56 dan 33,40 ± 10,13.  Persentase viabilitas spermatozoa adalah 29,10 ± 5,82; 35,34 ± 5,85; 42,52 ± 4,87; 51,76 ± 7,13 dan 48,6 ± 6,05. Penambahan ekstrak kulit buah naga merah konsentrasi 0,6% menghasilkan persentase motilitas dan viabilitas spermatozoa yang lebih baik dibandingkan konsentrasi perlakuan lainnya. Simpulan penambahan ekstrak kulit buah naga merah dalam pengencer sitrat kuning telur dapat meningkatkan persentase motilitas dan viabilitas spermatozoa sapi aceh setelah pembekuan.(This study aims to determine the effect of addition peels red dragon fruit extract (Hylocereus polyrhizus) in the yolk citrate diluent to the motility and viability of the aceh cattle spermatozoa after freezing. This study used fresh semen of aceh cattle collected using artificial vagina and divided into 5 groups of diluent treatments with 5 replications. The control group of cement was diluted in the yolk citrate dilator without the addition of red dragon fruit skin extract (P0), semen treatment group was diluted in the yolk citrate diluent plus  peels red dragon fruit extract 0.2% (P1), 0.4% (P2), 0.6% (P3) and 0.8% (P4). Data were analyzed by using analysis of variance (ANOVA) and continued by Duncan test. The results showed that the addition of peels red dragon fruit extract 0.0; 0.2; 0.4; 0.6 and 0.8%  of yolk citrate diluent yields motility percentage is 23.60 ± 4.72; 26.60 ± 5.94; 36.40 ± 4.98; 45.40 ± 4.56 and 33.40 ± 10.13. Percentage of spermatozoa viability were 29.10 ± 5.82; 35.34 ± 5.85; 42.52 ± 4.87; 51.76 ± 7.13 and 48.6 ± 6.05.The addition of peels red dragon fruit extract 0.6% yields better percentage of motility and spermatozoa viability than other treatment doses. The conclusion of addition peels red dragon fruit extract in yolk citrate diluent can increase the percentage motility and viability spermatozoa of aceh cattle after freezing)

12. The Effect Of Neem Leaves (Azadirachta Indica) Extract To The Blood Profile Of Male Rat (Rattus Norvegicus) Infected With Trypanosoma Evansi Of Krueng Raya Isolat

Jurnal Medika Veterinaria Vol 12, No 1 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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Abstract

This study aimed to determine the ability of neem leaf extract to maintain the normal blood profile of white male rat (Rattus norvegicus) infected with Trypanosoma evansi. A total of 24 male white rats were divided into 6 groups. The K0 group as the negative control received no T. evansi infection and no neem leaf extract. Furthermore, K1  group (positive control) only infected with T. evansi,  K2 and K3 were given neem leaf extract 50mg/kg BW and 100mg/kgBW, while K4 and K5 were given neem leaf extract 400mg/KgBW and  K5 800mg/kgBW. The neem leaf extract was administered for 3 consecutive days after infection established. The blood of the rats was collected to determine the amount of erythrocyte and leukocyte, hemoglobin level and hematocrit value. The data were analyzed using Analysis of Variance (ANOVA) through SPSS for Windows 16.0. The averages (+SD) of erythrocyte of K0, K1, K2, K3, K4, and K5 were 5,64±0,57; 2,31±1; 1,93±0; 0; 2,55±0,33; and 2,56±0,48. The amount of leukocyte were 4,46±1,09; 4,45±1,91; 5,25±0; 0; 8,42±1,66; and 8,14± 5,17. The value of hemoglobin were 12,00±0,47; 10,50±0,57; 4,30±0; 0; 5,60±0,20; and 9,03±0,66. The level of hematocrit 38,00±1,83; 25,00±9,90; 15±0; 0; 18,00±2,65, and 20,75±1,71. The result showed that the administration of neem leaf extract with doses 400mg/kgBW and 800mg/kgBW were difference significantly (P<0.05) compare to the positive control of rats, but could not equalize blood profile of uninfected white male rats