Sammy N.J Longdong
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Evaluation of The Use of Garlic (Allium sativum) in Enhancing Nonspecific Immune Response and Growth of Nile Tilapia (Oreochromis niloticus) Marentek, Gercyana; Manoppo, Henky; Longdong, Sammy N.J
e-Journal BUDIDAYA PERAIRAN Vol 1, No 1 (2013): Vol.1 No. 1 Januari 2013
Publisher : e-Journal BUDIDAYA PERAIRAN

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Abstract

The objective of this research was to examine the effect of garlic (Allium sativum) at different doses on nonspecific immune response and growth of nile tilapia (Oreochromis niloticus). Experimental fish was nile tilapia measuring 8-15 cm with an average weight of 10.4 g per individual. Before used in the experiment, fish were reared in fiber tank for two weeks for acclimatization. After acclimatization, the fish were moved into glass aquarium (50 cm x 30 cm x 30 cm) at the density of 15 fish/aquarium. Each aquarium was equipped with aerator. Five doses of garlic as treatments used in this research consisted of A= 0 g/kg diet, B= 10 g/kg, C= 20 g/kg, D= 30 g/kg and E= 40 g/kg. Fish was fed experimental diet at a dose of 5% of body weight per day, twice a day at 08.00 and 17.00. Data collected at the end of the experiment included immune parameters (TLC and phagocytosis activity) and growth of fish. ANOVA was used to evaluate the effect of treatments on immune response and growth of fish while Duncan Test was used to evaluate the different effect between treatments. Research result showed that after four weeks of feeding, TLC of fish fed diet supplemented with garlic significantly different (p<0,01) as compared to that of control fish. The highest number of TLC was observed at treatment C (15.413x107 cell.ml-1) followed by treatment B (15.39x107 cell.ml-1), D (13,920 x 107 cell.ml-1), E (13,582 x 107 cell.ml-1) and A (12,195 x 107 cell.ml-1). TLC of fish in treatment C was different significantly compared to control as well as to other treatments. Phagocytosis activity of fish in treatment C also increased significantly (p<0,01) compared to control. The highest PA was achieved in treatment C and then treatment B, D, E and A. Growth of fish in treatment C was different significantly compared to control fish as well as other treatments. As conclusion, oral administration of garlic at 20 g/kg diet could enhance nonspecific immune response and growth of nile tilapia. Key words: Garlic, Immune response, Total leukocyte count , Phagocytosis activity, Growth
Detection of the presence Aeromonas sp on nile tilapia cultured in floating net cage in lake Tondano Tantu, Wandalia; Tumbol, Reiny A; Longdong, Sammy N.J
e-Journal BUDIDAYA PERAIRAN Vol 1, No 3 (2013)
Publisher : e-Journal BUDIDAYA PERAIRAN

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Abstract

The purpose of this study was to detect the presence of bacteria Aeromonas sp in tilapia (Oreochromis niloticus). Nine fish with an average body weight of 150-200 g were used in this study as samples. Sampling was conducted at three different locations which were representative of fish culture sites located on Lake Tondano. The number of sample gathered from each location was and 3 fish which were taken randomly. Fish samples were taken by using a scoop, then the samples were taken alive by placing in an oxygen-filled plastic separately and taken direcly to lab Fish Disease Control and Environmental Center, Tateli, Department of Marine and Fisheries of North Sulawesi province, for examination the presence of bacteria. Isolation of bacteria were conducted by taking samples from gill and kidney. This study was carried out from April - July 2013. Identification of the presence of bacteria was done through a series of observations of colony morphology and gram staining of bacteria, followed by a series of biochemical tests: oxidase test , catalase test , test TSIA , H2S production test , indole test , motility test , citrate test, and test O/F. It could be concluded that 22.22 % of farmed nile tilapia in Lake Tondano were infected with the Aeromonas sp with the following percentage from each site: Paleloan village 7.40% , 7.40% Toulimembet village, and 7.40% Eris village. Keywords: Aeromonas sp, isolation, nile tilapia, floating net cage, Lake Tondano
Identifikasi Parasit pada Ikan Kerapu Sunu, Plectropomus leopardus Sauyai, Kristian; Longdong, Sammy N.J; Kolopita, Magdalena E.F
e-Journal BUDIDAYA PERAIRAN Vol 2, No 3 (2014)
Publisher : e-Journal BUDIDAYA PERAIRAN

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This research aimed to identify, analyze and determine the prevalence rate and dominance index of parasites on grouper (Plectropomus leopardus) landed at Bahu Beach. The number of samples used was 20 fishes measuring 230,68-490,10 g in weight and 24-29 cm in length. Ectoparasites were observer in mucus, fin, and gill while endoparasites were observed in peritoneal cavity and intestine of fish. Parasites invested grouper were Lernaeocera branchialis, Unitubulotestis sardae Acanthochondria cornuta, Mirasidium Schistosomatium douthiti, Argulus sp and  Gnathia maxillaris. All the parasites were found in fish gill and only one species in the mucus. Prevalence rate of parasites was 40%.  Based on parasite species, prevalence rate of  Unitubulotestis sardae was 20%, Acanthochondria cornuta 10%, Gnathia maxillaries 10%, Lernaeocera branchilia 5%, Mirasidium Schistosomatium douthiti 5% and Argulus sp 5%.  Parasites infected grouper dominated by three species namely Unitubulotestis sardae, Acanthochondria cornuta, and Gnathia maxillaris in which all the species were dominant in the gill.   Keywords :  Parasite, Plectropomus leopardus, prevalence rate, dominance index
Efektivitas Ekstrak Daun Pacar Air (Impatiens balsamina L.) Untuk Meningkatkan Respon Imun Non Spesifik Ikan Nila (Oreochromis niloticus) Pasaribu, Wesly; Longdong, Sammy N.J
e-Journal BUDIDAYA PERAIRAN Vol 3, No 1 (2015)
Publisher : e-Journal BUDIDAYA PERAIRAN

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Abstract

The objective of this research was  to determine the most effective dose and the best induction time of  Impatiens balsamina extract in enhancing nonspesific immune response of nile tilapia (Oreochromis niloticus).  The tested fish were nile tilapia, 14-16 cm in length, and  39-42 g in weight, obtained from Balai Pengembangan dan Pembinaan Pembudidayaan Ikan (BP3I) Tateli. The  research was designed in 2x4 factorial in completely randomized design.  There were  two factors   tested  in this research, the dose  and  time.  There were four levels of dose,  A1 = 0 mg/mL extract, A2= 30 mg/mL extract, A3= 50 mg/mL extract, A4= 70 mg/mL extract; and there were  two levels of time,  B1= 7 days after injection and B2=14 days after injection.   The extract was injected intramuscularly  with a dose of  0.2 mL per fish.   Data collected in this research was the immune parameters (total leucocyte count and phagocytosis activity). The results showed that the most effective dose in enhancing nonspesific immune response was   A1=50 mg/mL extract and the best induction time was  B1= 7 day after injection.   The results also indicated  that there was significant  interaction between  dose and time in influencing  the total amount of leucocyte, but there was not  interaction in influencing the phagocytic activities. Keywords : Impatiens balsamina, immune Response, total leucocyte count, phagocytosis activity.