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Gambaran Sel Eosinofil, Monosit, dan Basofil Setelah Pemberian Spirulina pada Ayam yang Diinfeksi Virus Flu Burung (OBSERVATION OF EOSINOPHILS, MONOCYTES, AND BASOPHILS AFTER TREATED WITH SPIRULINA IN CHICKENS THAT INFECTED WITH AVIAN INFLUENZA VIRUS) Lokapirnasari, Widya Paramita; Yulianto, Andreas Berny
Jurnal Veteriner Vol 15, No 4 (2014)
Publisher : Jurnal Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

High Pathogenecity Avian Influenza (HPAI) viruses have high virulence and can frequently causesudden death on birds. The aims of this research was to know the role of Spirulina to a number ofmonocytes and lymphocytes in the blood of chickens which infected with the H5N1 virus. This researchconsisted of three levels of treatment in which each level given Spirulina 0%, 10%, 20% in the fresh wateralgae as drinking water. Each treatment consisted of seven replicates, and the treatment was done sincethe chickens at age 19 until 44 days ( for 25 days). Artificial infection of the chickens with the virus waschallenged by using AI (H5N1) 104 EID 50 (A/Ck/Indonesia/BL/03) with route to the respiratory tract (nosedrops) 0,1 mL starting on day 19. The results showed that there were a significant difference (p<0.05) ontreatment that given Spirulina at doses of 0%, 10% and 20% for the number ofn monocytes, eosinophils,whereas no significant difference (p > 0.05) was observed in basophils.
POTENSI KOMBINASI BAKTERI DAN JAMUR SELULOLITIK PADA FERMENTASI BEKATUL TERHADAP KANDUNGAN SERAT KASAR DAN PROTEIN KASAR Lokapirnasari, Widya Paramita; Setiawan, Agus; Prawesthirini, Soetji
Buletin Peternakan Vol 39, No 3 (2015): BULETIN PETERNAKAN VOL. 39 (3) OKTOBER 2015
Publisher : Faculty of Animal Science UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Penelitian ini bertujuan untuk mengetahui kandungan serat kasar dan protein kasar pada bekatul padi yang difermentasi dengan bakteri selulolitik dan jamur selulolitik. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap pola searah dengan sepuluh perlakuan dan masing-masing perlakuan dilakukan 3 kali ulangan. Ke sepuluh perlakuan tersebut adalah P0: 250 g bekatul + 3% tetes + Bakteri (B) 0% + Jamur (J) 0%; P1: 250 g bekatul + 3% tetes +B.10% + J.10%; P2: 250 g bekatul + 3% tetes + B.20% + J.10%; P3: 250 g bekatul + 3% tetes + B.30% + J.10%; P4: 250 g bekatul + 3% tetes + B.10% + J.20%; P5: 250 g bekatul + 3% tetes + B.20% + J.20%; P6: 250 g bekatul + 3% tetes + B.30% + J.20%; P7: 250 g bekatul + 3% tetes + B.10% + J.30%; P8: 250 g bekatul + 3% tetes + B.20% + J.30%; P9: 250 gbekatul + 3% tetes + B.30% + J.30%. Setelah dilakukan fermentasi selama 7 hari, dilakukan analisis proksimat. Data yang diperoleh selanjutnya diuji dengan analisis variansi, dilanjutkan dengan uji Duncan’s Multiple Range Test. Hasil penelitian menunjukkan bahwa bakteri selulolitik dan jamur selulolitik dapatmenurunkan kandungan serat kasar pada bekatul pada perlakuan P2 (28,96%), P3 (29,34%), P8 (29,53%), P4 (29,65%), P7 (30,23%), P6 (30,37%), P9 (30,58%) yang berbeda nyata (P<0,05) dengan perlakuan P5 (31,10%), P1 (31,98%), P0 (34,1 1%), serta dapat meningkatkan kandungan protein kasar pada perlakuan P2 (13,97%), P5 (12,87%), P3 (12,84%), P8 (12,74%) yang berbeda (P<0,05) dengan perlakuan P7 (12,71 %), P6 (12,44%), P4 (12,36%), P9 (12,27%), P1 (12,25%), P0 (10,9%).(Kata kunci: Acidothermus cellulolyticus, Aspergillus terreus, Bekatul, Fermentasi, Protein kasar, Serat kasar)
Potensi Bakteri Lactococcus sp. dan Lactobacillus sp. untuk Peningkatan Kualitas Limbah Kulit Kacang Sebagai Alternatif Bahan Pakan [Potential of Lactococcus sp. and Lactobacillus sp. Bacteria for Quality Improvement of Peanut Peel Waste as Alternative Feed Ingredients] Lokapirnasari, Widya Paramita; Widodo, Oky Setyo; Koestanti, Emy
Jurnal Ilmiah Perikanan dan Kelautan Vol 10, No 1 (2018): Jurnal ilmiah perikanan dan kelautan
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.226 KB) | DOI: 10.20473/jipk.v10i1.8547

Abstract

                                                    AbstrakTujuan penelitian ini untuk menterminasi peningkatan kandungan nutrien dalam fermentasi makanan yang terdapat dari kulit kacang sebagai bahan pakan alternatif untuk ternak dan ikan. Penelitian ini terdiri dari tiga perlakuan dan lima replikasi P0: kulit kacang+isolat 0%; P1: kulit kacang+1% Lactococus sp. ; P2 : kulit kacang+1% Lactobacillus sp. Proses fermentasi dilakukan selama 5 hari dalam keadaan aerob. Hasil menunjukan bahwa terdapat berdedaan yang signifikan antara perlakuan (p<0,05). Penggunaan isolat Lactococcus sp. dan Lactobacillus sp. dapat meningkatkan kandungan nutrient dari limbah kulit kanang. Terdapat penurunan aerob mentah dari 3,80 sampai 7,70% terdapat peningkatan elestrak nitrogen bebas dari 3,925 sampai 4,38% terdapat peningkatan kandungan energi pencernaan 7.13% sampai 9.30%. berdasarkan hasil penelitian dapat disimpulkan bahwa pengguna 1% Lactococcus sp. dan 1% Lactococcus sp. dapat meningkatkan nilai nutrisi di limbah kulit kacang yang dapat digunakan sebagai alternatif bahan pakan ternak dan ikan.                                                   AbstractThis study aims to determine the increasing of nutrient content in the fermentation of feed stuff derived from peanut wastes as an alternative feed material for livestock and fish. The study consisted of three treatments and five replications, namely P0: peanut peel + 0% isolate, P1: peanut peel + 1% Lactococcus sp, P2: peanut peel +1% Lactobacillus sp. The fermentation process was carried out for 5 days under a facultative anaerobic condition. The results showed that there was a significant difference between treatments (p <0.05). The use of Lactococcus sp and Lactobacillus sp isolates can increase the nutritional content of peanut peel wastes. There was a decrease in crude fiber content (CF) of 3.80 to 7.70%, there was an increase of Nitrogen free extract (NFE) of 3.925 to 4.38%, there is an increase in digestible energy (DE) content of 7.13% to 9.30%, there was the increasing metabolizable energy (ME) of 7.13% to 9.12% compared to control. Based on the results it can be concluded that the using of 1% Lactococcus sp and 1% Lactobacillus sp. can increase nutritional value of peanut peel waste that can be used as an alternative ingredients of animal and fish.
POTENSI KOMBINASI BAKTERI DAN JAMUR SELULOLITIK PADA FERMENTASI BEKATUL TERHADAP KANDUNGAN SERAT KASAR DAN PROTEIN KASAR Lokapirnasari, Widya Paramita; Setiawan, Agus; Prawesthirini, Soetji
Buletin Peternakan Vol 39, No 3 (2015): BULETIN PETERNAKAN VOL. 39 (3) OKTOBER 2015
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.21059/buletinpeternak.v39i3.7985

Abstract

Penelitian ini bertujuan untuk mengetahui kandungan serat kasar dan protein kasar pada bekatul padi yang difermentasi dengan bakteri selulolitik dan jamur selulolitik. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap pola searah dengan sepuluh perlakuan dan masing-masing perlakuan dilakukan 3 kali ulangan. Ke sepuluh perlakuan tersebut adalah P0: 250 g bekatul + 3% tetes + Bakteri (B) 0% + Jamur (J) 0%; P1: 250 g bekatul + 3% tetes +B.10% + J.10%; P2: 250 g bekatul + 3% tetes + B.20% + J.10%; P3: 250 g bekatul + 3% tetes + B.30% + J.10%; P4: 250 g bekatul + 3% tetes + B.10% + J.20%; P5: 250 g bekatul + 3% tetes + B.20% + J.20%; P6: 250 g bekatul + 3% tetes + B.30% + J.20%; P7: 250 g bekatul + 3% tetes + B.10% + J.30%; P8: 250 g bekatul + 3% tetes + B.20% + J.30%; P9: 250 gbekatul + 3% tetes + B.30% + J.30%. Setelah dilakukan fermentasi selama 7 hari, dilakukan analisis proksimat. Data yang diperoleh selanjutnya diuji dengan analisis variansi, dilanjutkan dengan uji Duncan’s Multiple Range Test. Hasil penelitian menunjukkan bahwa bakteri selulolitik dan jamur selulolitik dapatmenurunkan kandungan serat kasar pada bekatul pada perlakuan P2 (28,96%), P3 (29,34%), P8 (29,53%), P4 (29,65%), P7 (30,23%), P6 (30,37%), P9 (30,58%) yang berbeda nyata (P<0,05) dengan perlakuan P5 (31,10%), P1 (31,98%), P0 (34,1 1%), serta dapat meningkatkan kandungan protein kasar pada perlakuan P2 (13,97%), P5 (12,87%), P3 (12,84%), P8 (12,74%) yang berbeda (P<0,05) dengan perlakuan P7 (12,71 %), P6 (12,44%), P4 (12,36%), P9 (12,27%), P1 (12,25%), P0 (10,9%).(Kata kunci: Acidothermus cellulolyticus, Aspergillus terreus, Bekatul, Fermentasi, Protein kasar, Serat kasar)
Pengaruh Subtitusi Tepung Biji Bunga Matahari (Helianthus Annuus L.) dalam Pakan Komersial dengan Konsentrasi Tertentu Terhadap Performa Ayam Pedaging Hermansyah, Beta; Lokapirnasari, Widya Paramita; Fikri, Faisal
Jurnal Medik Veteriner Vol 2, No 1 (2019): Jurnal Medik Veteriner
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol2.iss1.2019.7-12

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh penggantian tepung biji bunga matahari (Helianthus annuus L.) di pakan komersial dengan konsentrasi tertentu pada kinerja ayam pedaging. Penelitian ini menggunakan desain acak lengkap. Sampel terdiri dari 20 ekor ayam broiler dibagi secara acak menjadi 5 kelompok. Perlakuan adalah P1, P2, P3, dan P4 masing-masing CP511 pakan komersial yang mengandung tepung biji bunga matahari 25%, 30%, 35%, dan 40% serta kontrol tanpa substitusi P0 tepung biji bunga matahari. Variabel yang digunakan adalah konsumsi pakan, bobot akhir dan konversi pakan kemudian hasilnya dianalisis dengan Analisis Varians. Hasil yang diperoleh rata-rata nilai konsumsi pakan P0 adalah 79,76 gram, P1 adalah 77,90 gram, P2 adalah 77,35 gram, P3 adalah 77,98 gram, P4 adalah 77,58 gram. Hasil nilai rata-rata berat akhir 1545,5 gram yaitu P0, P1 adalah 1296,5 gram, P2 1325,75 gram, P3 1256 gram, P4 1203 gram. Hasil yang diperoleh dari nilai rata-rata konversi pakan adalah P0 1,37, P1 1,69, P2 1,61, P3 1,71, P4 1,88. Berdasarkan hasil dapat disimpulkan bahwa substitusi tepung biji bunga matahari dalam diet komersial tidak meningkatkan asupan pakan, berat badan dan dapat meningkatkan konversi pakan.
Pemberdayaan Masyarakat Melalui Aplikasi Teknologi Inseminasi Buatan, Pengolahan Pakan , Biofarmaka dan Limbah dalam Upaya Pengembangan Sentra Kambing maslachah, lilik; Suprayogi, Tri Wahyu; Lokapirnasari, Widya Paramita
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 4, No 2 (2019): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.28219

Abstract

ABSTRACT Community empowerment of the goat farming group in Kerek and Merakurak, Tuban aims to improve the knowledge of artificial insemination technology of goats, processing agricultural and plantation waste products for goat feed,processing and using medicinal plants and processing goat’s faeces waste to become environmentally-friendly fertilizer. Methods: observing the location, interviewing and discussing with the leader of the group to clarify the problems faced by goat farmers. Education given by seminar and training by demos of artificial insemination, complete feed processing, bio-pharmaceutical preparation and waste processing. Evaluation and monitoring the success of the sustainability program cooperating with local animal husbandry department for assistance by field operators serving on the area. The output of TTG is transformation of artificial insemination technology of goats. Knowledge and understanding of farmers about how to process complete feed for goats. Making bio pharmaceutical preparation independently. Production of environmentally-friendly fertilizer. Keywords: artificial insemination; bio-pharmaceutical; complete feed; waste product
Pemberdayaan Masyarakat Melalui Aplikasi Teknologi Inseminasi Buatan, Pengolahan Pakan , Biofarmaka dan Limbah dalam Upaya Pengembangan Sentra Kambing Maslachah, Lilik; Suprayogi, Tri Wahyu; Lokapirnasari, Widya Paramita
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 4, No 2 (2019): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.28219

Abstract

ABSTRACT Community empowerment of the goat farming group in Kerek and Merakurak, Tuban aims to improve the knowledge of artificial insemination technology of goats, processing agricultural and plantation waste products for goat feed,processing and using medicinal plants and processing goat’s faeces waste to become environmentally-friendly fertilizer. Methods: observing the location, interviewing and discussing with the leader of the group to clarify the problems faced by goat farmers. Education given by seminar and training by demos of artificial insemination, complete feed processing, bio-pharmaceutical preparation and waste processing. Evaluation and monitoring the success of the sustainability program cooperating with local animal husbandry department for assistance by field operators serving on the area. The output of TTG is transformation of artificial insemination technology of goats. Knowledge and understanding of farmers about how to process complete feed for goats. Making bio pharmaceutical preparation independently. Production of environmentally-friendly fertilizer. Keywords: artificial insemination; bio-pharmaceutical; complete feed; waste product
PENGARUH PEMBERIAN PROBIOTIK Lactobacillus acidophilus DAN Bifidobacte-rium sp TERHADAP PERFORMA PRODUKSI AYAM PETELUR YANG DIINFEKSI Escherichia coli Huda, Khoirul; Lokapirnasari, Widya Paramita; Soeharsono, Soeharsono
Jurnal Ilmiah Teknosains Vol 5, No 1 (2019): JiTek
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/jitek.v5i1.3459

Abstract

The purpose of this study is to determine the use of probiotics Lactobacillus acidophilus and Bifidobacterium sp on feed consumption and Hen Day Production (HDP) laying hens which infected by Escherichia coli. Arround 120 layers at the age of 25 weeks are divided into six treatments. Each treatment consisted of factor a (infection and non-infection Escherichia coli) and factor b (basal feed, AGP and probiotics). The result of this study shows that giving probiotic has taken an effect (p<0,05) on the feed consumption and HDP. The infection factor takes effect (p <0.05) on HDP but it does not take effect (p>0,05) on the feed consumption. There is interaction among probiotic factor and infection (p>0.05) on HDP but it has no effect (p> 0.05) on feed consumption. The highest feed consumption is found in the giving of non-infectious probiotics that is 114.7 g and the highest HDP which is from the giving of non-infectious probiotics is 98.6 %. In conclusion, the giving of probiotic Lactobacillus acidophilus and Bifidobacterium sp are able to produce well perform product and the high profit.
PENAMBAHAN PROBIOTIK LACTOBACILLUS ACIDOPHILUS DAN BIFIDOBACTERIUM SP TERHADAP ANALISIS USAHA AYAM PETELUR YANG DIINFEKSI ESCHERICHIA COLI Huda, Khoirul; Lokapirnasari, Widya Paramita; Soeharsono, Soeharsono
Mimbar Agribisnis: Jurnal Pemikiran Masyarakat Ilmiah Berwawasan Agribisnis Vol 5, No 2 (2019): Juli 2019
Publisher : Faculty of Agriculture, University of Galuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/ma.v5i2.1931

Abstract

The purpose of this study was to determine the use of probiotics Lactobacillus acidophilus and Bifidobacterium sp on variable cost, income and profit layers hens was infected with Escherichia coli. About 120 layers at 25 weeks of age were randomized into six treatments. The treatment consisted of factors a (infection and non-infection Escherichia coli) and factor b (basal feed, AGP and probiotics). The results of the study gave probiotics an effect (p <0.05) on variable cost, income and profit. Infection factors have an effect (p <0.05) on income and profit, but have no effect (p> 0.05) on variable cost. There was interaction between probiotic and infection factors (p <0.05) on income and profit, but there was no interaction (p> 0.05) between probiotic and infection factors on variable cost. The highest variable cost was found in the treatment of non-infectious probiotics is Rp 13.594, the highest income was treated with non-infectious probiotics is Rp 26.564, the highest profit was treated with non-infectious probiotics is Rp 12.969. It could be concluded that the administration of probiotics can produce good production performance.
PENGGUNAAN PROBIOTIK KOMBINASI Lactococcus lactis DAN Lactobacillus acidophilus SEBAGAI PENGGANTI ANTIBIOTIKA PADA AYAM PETELUR YANG DIINFEKSI Escherichia coli TERHADAP ANALISIS USAHA Wardhani, Hana Cipka Pramuda; Lokapirnasari, Widya Paramita; Soepranianondo, Koesnoto
Mimbar Agribisnis: Jurnal Pemikiran Masyarakat Ilmiah Berwawasan Agribisnis Vol 5, No 2 (2019): Juli 2019
Publisher : Faculty of Agriculture, University of Galuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/ma.v5i2.1949

Abstract

Escherichia coli is a normal flora in the digestive system of laying hens that are non-pathogenic, which can change into pathogens and cause the egg production to decrease. So the combination of Lactococcus lactis and Lactobacillus acidophilus probiotics is expected to be able to overcome E. coli and become a substitute for the  antibiotics (Virginiamycin) in animal feed. This study aims to determine business analysis including Break Event Points (BEP), Revenue Cost Ratio (R / C Ratio), Payback Period (PP) and Return On Investment (ROI). The best results obtained for the calculation of Break Event Point (BEP) on a0b2 treatment with a BEP of Rp. 17,587,24 with BEP production on a1b2 of 14,36 kg, Revenue Cost Ratio (R / C Ratio) generates a value of 1,543 for treatment a0b2, Payback Period (PP) generates a value of 1 year 3 months 9 days and Return On Investment (ROI) generates a value of 3. It was concluded that the a0b2 treatment had good results to be developed.