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Pertumbuhan Bibit Tanaman Manggis (Garcinia mangostana L.) Setelah Inokulasi dengan Berbagai Galur Agrobacterium rhizogenes1 Lizawati, ,; Poerwanto, Roedhy; Sobir, ,; Rusmana, Iman; Ermayanti, Tri Muji
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 35, No 2 (2007): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Growth of mangosteen essentially depends on its root system.  Therefore, it needs technology to obtain stringer mangosteen root system.  The use of Agrobacterium rhizogenes bacterium is an alternative.  The objectives of this experiment were : 1) to find the effective strain of A. rhizogenes bacterium for inoculation of mangosteen seedling root, 2) to find the best inoculation method for inducing mangosteen seedling root.  The materials used in this experiment were ; mangosteen fruit and A. rhizogenes collection from Puslit Biotechnology LIPI Cibinong-Bogor.  The experiment was arranged in completely randomized design with two factorial treatments.  The first factor : 11 strains A. rhizogenes (ATCC-15834, ATCC-8196, R-1000, 07-20001, A4, A4-J, 509, 510, 511, MAFF 01-1724, and control), the second factor : 2 inoculation methods (cutting and dipping).  The results showed that A. rhizogenes  of ATCC-15834, 509, 07-20001, A4, and R-1000 increased stem diameter, plant height, leaf number, lateral and tertiary root number, better than ATCC-8196, MAFF 01-1724, 510, 511, A4-J, and control.  Cutting root method of inoculation resulted in higher live plant percentage compared to dipping root method.   Key words :  Agrobacterium rhizogenes, Garcinia mangostana, inoculation
Induksi dan Multiplikasi Tunas Jarak Pagar (Jatropha curcas L.) Secara In Vitro Lizawati, ,; Novita, Trias; Purnamaningsih, Ragapadmi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 37, No 1 (2009): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The conventional propagation of physic nut (Jatropha curcas) is difficult, because it requires a high number of mother plant, which is very limited. In vitro culture is an alternative technique to conventional one to solve the problem.  An experiment was done to obtain the best in vitro culture media for shoot induction and multiplication. This research was separated into two steps, (1) in vitro induction of explant growth, and (2) in vitro shoot multiplication.  Results showed that medium of WPM + 2.0 ppm BAP induced shoot and leaf better than the control.  The highest number of leaf axillary´s multiplication was obtained from the medium WPM + 2.0 ppm BAP + 0.1 ppm NAA. Various medium formulations for the induction and multiplication of shoots resulted in highly leaf fall.  The use of DKW + 2.0 ppm BAP + 0.4 ppm TDZ + 3.0 ppm AgNO3 medium has effectively induced shoot multiplication and reduction of dehydrated leaf. Meanwhile, the used of DKW medium supplemented with 5 ppm kinetin resulted in the best shoot elongation.   Key words :  Induction, in vitro, Jatropha curcas, shoot, multiplication
Induksi dan Multiplikasi Tunas Jarak Pagar (Jatropha curcas L.) Secara In Vitro Lizawati, ,; Novita, Trias; Purnamaningsih, Ragapadmi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 37, No 1 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

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Abstract

The conventional propagation of physic nut (Jatropha curcas) is difficult, because it requires a high number of mother plant, which is very limited. In vitro culture is an alternative technique to conventional one to solve the problem.? An experiment was done to obtain the best in vitro culture media for shoot induction and multiplication. This research was separated into two steps, (1) in vitro induction of explant growth, and (2) in vitro shoot multiplication.? Results showed that medium of WPM + 2.0 ppm BAP induced shoot and leaf better than the control.? The highest number of leaf axillary's multiplication was obtained from the medium WPM + 2.0 ppm BAP + 0.1 ppm NAA. Various medium formulations for the induction and multiplication of shoots resulted in highly leaf fall.? The use of DKW + 2.0 ppm BAP + 0.4 ppm TDZ + 3.0 ppm AgNO3 medium has effectively induced shoot multiplication and reduction of dehydrated leaf. Meanwhile, the used of DKW medium supplemented with 5 ppm kinetin resulted in the best shoot elongation. ? Key words :? Induction, in vitro, Jatropha curcas, shoot, multiplication
Pertumbuhan Bibit Tanaman Manggis (Garcinia mangostana L.) Setelah Inokulasi dengan Berbagai Galur Agrobacterium rhizogenes1 Lizawati, ,; Poerwanto, Roedhy; Sobir, ,; Rusmana, Iman; Ermayanti, Tri Muji
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 35, No 2 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

Show Abstract | Original Source | Check in Google Scholar

Abstract

Growth of mangosteen essentially depends on its root system.? Therefore, it needs technology to obtain stringer mangosteen root system.? The use of Agrobacterium rhizogenes bacterium is an alternative.? The objectives of this experiment were : 1) to find the effective strain of A. rhizogenes bacterium for inoculation of mangosteen seedling root, 2) to find the best inoculation method for inducing mangosteen seedling root.? The materials used in this experiment were ; mangosteen fruit and A. rhizogenes collection from Puslit Biotechnology LIPI Cibinong-Bogor.? The experiment was arranged in completely randomized design with two factorial treatments.? The first factor : 11 strains A. rhizogenes (ATCC-15834, ATCC-8196, R-1000, 07-20001, A4, A4-J, 509, 510, 511, MAFF 01-1724, and control), the second factor : 2 inoculation methods (cutting and dipping).? The results showed that A. rhizogenes ?of ATCC-15834, 509, 07-20001, A4, and R-1000 increased stem diameter, plant height, leaf number, lateral and tertiary root number, better than ATCC-8196, MAFF 01-1724, 510, 511, A4-J, and control.? Cutting root method of inoculation resulted in higher live plant percentage compared to dipping root method. ? Key words : ?Agrobacterium rhizogenes, Garcinia mangostana, inoculation
Callus Proliferation from Immature Leaf Explants of Durian (Durio zibethinus Murr. cv. Selat) with the Addition of Picloram and BAP ,, Zulkarnain; Lizawati, ,
Jurnal Hortikultura Indonesia Vol 4, No 3 (2013): Jurnal Hortikultura Indonesia
Publisher : Perhimpunan Hortikultura Indonesia dan Departemen Agronomi dan Hortiluktura

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Abstract

ABSTRACTThis study was aimed to obtain an appropriate medium composition with various combinations of Picloram + BAP for the proliferation of embryogenic callus from immature leaf explants of durian. The experiment was carried out at the Plant Biotechnology Laboratory,  Faculty of Agriculture ,  the University of Jambi from January through to November 2012. Five levels of Picloram (1.0, 2.0, 3.0, 4.0, 5.0 mg  L-1) in combination with three levels of BAP (0, 0.5, 1.5 mg  L-1) were tested. Therefore, there were 15 treatment combinations with 4 replicates resulting in  60 experimental unit. Each unit  consisted of 4 culture flasks containing one immature leaf explant. Cultures were kept in culture room with 16 h photoperiod and 1000 lux light intensity. The results showed that: 1) callus proliferation on immature leaf explants of durian cv. Selat was dependent upon the level of Picloram + BAP added to culture medium, 2) the addition of 3.0 -  5.0 mg L-1Picloram without BAP was found to be effective in promoting callus proliferation on the majority of cultured explants, 3) all regenerated callus showed similar characteristics, but embryogenic properties was not seen yet, and 4) the application of tissue culture technique in the propagation of durian cv. Selat needs further comprehensive  investigation, particularly on factors directly affecting culture development and inducing somatic embryogenesis.Key words: tissue culture, in vitro culture, micropropagation, plant hormones, auxin, cytokinin, fruit crops. ABSTRAKPenelitian  ini  bertujuan  untuk  mendapatkan komposisi  media  yang  tepat dari  kombinasi Picloram + BAP untuk proliferasi kalus embriogenik dari eksplan daun dewasa durian. Penelitian dilakukan  di  Laboratorium  Bioteknologi Tanaman,  Fakultas  Pertanian,  Universitas  Jambi  dari Januari hingga November 2012. Perlakuan kombinasi media zat pengatur tumbuh adalah lima taraf Picloram (1.0, 2.0, 3.0, 4.0, 5.0 mg L-1) dengan kombinasi tiga taraf perlakuan BAP (0, 0.5, 1.5 mg L-1).  Terdapat  15  kombinasi  media perlakuan dengan  4  ulangan,  sehingga  terdapat  60  kombinasi satuan percobaan. Setiap unit percobaan terdapat 4 botol kultur dengan satu eksplan daun. Kultur disimpan di ruang kultur selama 16 jam penyinaran dan intensitas cahaya 1000  lux. Hasil penelitian menunjukkan bahwa: 1) Proliferasi kalus dari eksplan daun  muda buah  durian tergantung pada taraf kombinasi  picloram  +  BAP yang  ditambahkan  ke  media  kultur;  2)  Penambahan  3.0-5.0  mg  L-1Picloram  tanpa  BAP  memberikan  hasil  yang  efektif  untuk  menginduksi proliferasi  kalus  pada sebagian besar eksplan; 3)  Regenerasi kalus menunjukkan karakteristik serupa, tetapi embriogenik kalus  tidak  muncul, dan 4)  Perbanyakan  eksplan  daun  durian  dengan  tehnik  kultur jaringan membutuhkan penelitian lebih  lanjut, terutama pada faktor yang berpengaruh langsung pada induksi embriogenesis somatik.Kata kunci: auksin, buah, kultur jaringan, kultur in vitro, mikropropagasi, sitokinin, zat pengaturtumbuh,