Yulianus Linggi
Jurusan Budidaya Perairan Fakultas Perikanan dan Ilmu Kelautan Universitas Brawijaya, Malang

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Journal : Jurnal Kedokteran Hewan

Maturation of Nile-Tilapia (Oreochromis niloticus) Oocytes With Exposed by Goat Inhibin Isolated from Granulose Cells Linggi, Yulianus; A, Aulanni’am; Risjani, Yenni; N. Siregar, Tongku
Jurnal Kedokteran Hewan Vol 1, No 1 (2007): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.88 KB) | DOI: 10.21157/j.ked.hewan.v1i1.3118

Abstract

The present study aimed to investigate the role of inhibin in the regulation of oocytesmaturation. Inhibin was isolated from goat granulose cells and injected by intraperitoneal in to femaleNile-Tilapia. Three days later its gonad was taken by decapitation and the change of oocyte was evaluatedunder microscope. Results showed that average of percentages of oocytes that undergo maturated after exposed with inhibin of 0, 20, 40, and 60µg/individual 50.05, 29.44, 21.46, and 30.83%, respectively.The exposure of doses of goat inhibin in Nile-Tilapia showed significantly different (P<0.01) to percentages of mature oocytes. It was conluded that exposition of goat inhibin from granulose cells caninducing the maturation of Nile-Tilapia oocytes.Keywords: inhibin, maturation, oocytes
KEMAMPUAN OOSIT IKAN LELE (Clarias grapienus) DALAM MENOLERANSI KLORIN SEBAGAI BAHAN OKSIDATIF STRES Armansyah, Hadid; Linggi, Yulianus; M, Marsoedi
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (345.908 KB) | DOI: 10.21157/j.ked.hewan.v8i1.1256

Abstract

Penelitian ini menggunakan klorin dalam bentuk natrium hipoklorit yang dipaparkan secara in vitro untuk menguji kemampuan oosit ikan lele dalam menoleransi kehadiran klorin sebagai bahan oksidatif stres dalam medium kultur. Konsentrasi klorin yang digunakan adalah 0, 10, 20, 40, 80, 160, dan 320 μM. Hasil penelitian menunjukkan bahwa semakin tinggi konsentrasi paparan semakin rendah jumlah oosit yang mampu bertahan hidup hingga 4 jam pengamatan. Konsentrasi klorin 320 μM menghasilkan jumlah oosit yang mampu bertahan hidup paling optimum. Disimpulkan bahwa oosit ikan lele mampu menoleransi kehadiran bahan oksidatif stres dalam medium kultur dengan konsentrasi paparan klorin sebanyak 320 μM.