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POTENSI GENISTEIN PADA SISTEM REPRODUKSI MENCIT JANTAN (Mus musculus)

Prosiding Seminar Biologi Vol 8, No 1 (2011): Seminar Nasional VIII Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

ABSTRAK Usaha untuk mendapatkan sarana kontrasepsi pria sebenarnya telah banyak dilakukan dengan memanfaatkan bahan alami yang berasal dari tanaman. Salah satunya adalah tanaman famili Leguminoceae yang mengandung senyawa genistein. Genistein sebagai salah satu senyawa derivat isoflavon mempuyai struktur kimia mirip dengan 17β-estradiol yang bersifat seperti hormon steroid estrogen, yang mampu menyebabkan kerusakan pada sistem reproduksi jantan sebagai salah satu indikator digunakannya genistein sebagai senyawa antifertilitas. Penelitian ini bertujuan untuk mengetahui pengaruh genistein terhadap sistem reproduksi mencit jantan (Mus musculus). Penelitian ini menggunakan pendekatan kuantitatif eksperimental, yang rancangannya mengikuti Rancangan Acak Kelompok. Variabel bebas adalah dosis genistein 0 mg/g, 0,0035 mg/g, 0,0042 mg/g, dan 0,0049 mg/g. Variabel terikatnya adalah sel-sel germinal dalam tubulus seminiferus testis, morfologi spermatozoa, viabilitas spermatozoa, dan kadar hormon testosteron. Data sel-sel germinal dalam tubulus seminiferus testis kadar hormon testosteron dianalisis menggunakan Analisis Varians Satu Jalan (One Way ANOVA) dengan tingkat signifikansi 5%. Selanjutnya dilakukan uji Post Hoc dengan Least Significant Difference (LSD) 5%. Data morfologi dan viabilitas spermatozoa dinyatakan dalam prosentase. Hasil penelitian menunjukkan bahwa ada pengaruh genistein pada sistem reproduksi mencit jantan (Mus musculus). Genistein yang diberikan dengan dosis 0,0035 mg/g; 0,0042 mg/g; dan 0,0049 mg/g berpengaruh terhadap jumlah sel germinal, morfologi dan viabilitas spermatozoa. Kadar hormon testosteron berbeda nyata pada dosis 0,0049 mg/g. Kata kunci : genistein, sistem reproduksi jantan

POTENSI GENISTEIN PADA SISTEM REPRODUKSI MENCIT JANTAN (Mus musculus)

Prosiding Seminar Biologi Vol 8, No 1 (2011): Seminar Nasional VIII Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Original Source | Check in Google Scholar | Full PDF (412.028 KB)

Abstract

ABSTRAK Usaha untuk mendapatkan sarana kontrasepsi pria sebenarnya telah banyak dilakukan dengan memanfaatkan bahan alami yang berasal dari tanaman. Salah satunya adalah tanaman famili Leguminoceae yang mengandung senyawa genistein. Genistein sebagai salah satu senyawa derivat isoflavon mempuyai struktur kimia mirip dengan 17β-estradiol yang bersifat seperti hormon steroid estrogen, yang mampu menyebabkan kerusakan pada sistem reproduksi jantan sebagai salah satu indikator digunakannya genistein sebagai senyawa antifertilitas. Penelitian ini bertujuan untuk mengetahui pengaruh genistein terhadap sistem reproduksi mencit jantan (Mus musculus). Penelitian ini menggunakan pendekatan kuantitatif eksperimental, yang rancangannya mengikuti Rancangan Acak Kelompok. Variabel bebas adalah dosis genistein 0 mg/g, 0,0035 mg/g, 0,0042 mg/g, dan 0,0049 mg/g. Variabel terikatnya adalah sel-sel germinal dalam tubulus seminiferus testis, morfologi spermatozoa, viabilitas spermatozoa, dan kadar hormon testosteron. Data sel-sel germinal dalam tubulus seminiferus testis kadar hormon testosteron dianalisis menggunakan Analisis Varians Satu Jalan (One Way ANOVA) dengan tingkat signifikansi 5%. Selanjutnya dilakukan uji Post Hoc dengan Least Significant Difference (LSD) 5%. Data morfologi dan viabilitas spermatozoa dinyatakan dalam prosentase. Hasil penelitian menunjukkan bahwa ada pengaruh genistein pada sistem reproduksi mencit jantan (Mus musculus). Genistein yang diberikan dengan dosis 0,0035 mg/g; 0,0042 mg/g; dan 0,0049 mg/g berpengaruh terhadap jumlah sel germinal, morfologi dan viabilitas spermatozoa. Kadar hormon testosteron berbeda nyata pada dosis 0,0049 mg/g. Kata kunci : genistein, sistem reproduksi jantan

PENINGKATAN KUALITAS PEMBELAJARAN FISIOLOGI HEWAN DENGAN PENGEMBANGAN BAHAN AJAR BERKARAKTER BERBASIS HASIL PENELITIAN

Bioedukasi Jurnal Pendidikan Biologi Vol 4, No 2 (2013): Nopember 2013
Publisher : UNIVERSITAS MUHAMMADIYAH METRO

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Abstract

This study aims to improve the quality of learning by developing character-based teaching materials research animal physiology courses. This research method is the development of module manufacturing as a source of learning based on the research that has been done, including a description, picture research and implementation process that include sites expected character, whether written or unwritten is adapted from Dick and Carey learning system to make some adjustments. The results obtained are based on the calculation and conversion of valuation module by module expert instructional materialsby 71, 66%. Based on the results of the conversion, the preparation of the module other wise well qualified and do not need to be revised. The results of the assessment calculation and conversion modules by physiologists by 85%. Based on the results of  the conversion, the module qualification other wise excellent preparation and do not need to be revised. Conclusion are obtained the results by the developing of characterized teaching materials based on the results of experiment is feasible and can be used to support lectures to enhance the quality of teaching Animal Physiology courses in college.Kata kunci: Kualitas pembelajaran fisiologi hewan, bahan ajar berkarakter 

PENGEMBANGAN BAHAN AJAR BERBASIS PENELITIAN KARAKTERISASI PROTEIN MEMBRAN SPERMA PADA MATAKULIAH BIOTEKNOLOGI

Jurnal Florea Vol 2, No 2 (2015)
Publisher : Jurnal Florea

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Abstract

The purpose of the research and development is to produce teaching materials such as textbookssupporting course in Biotechnology research based on the characterization of the sperm membraneproteins for students who take a course in Biotechnology. This research is a research and development, where the results of research become the teaching materials developed supporting Biotechnologycourse. Development models have prepared using ADDIE Model. Sources of data in this researchinclude laboratory research and validation of the results sheet of Biotechnology material experts,instructional design experts, the media, and the trials in students. The analysis is used descriptiveanalysis and quantitative descriptive exploratory. The results of the research have developed becometeaching materials like text books supporting courses and have been validated by the Biotechnology,the biotechnology material experts, instructional design experts, the media, the individual testing, andgroup trials. Based on the results of the validation material have obtained value of 89.56%, the resultsof the design validation and media experts to get the value of 93.75%, it means that the teachingmaterials has been developed which suitable to be used as a teaching material support biotechnologycourse. While the experiments performed to get a data the percentage of students that are equal to88.98% and included in the category of “very good”, but still need to be revised based on advices andcomments from students.Keywords: Teaching material, Characterization of Membrane Proteins, Biotechnology. 

PENGEMBANGAN BAHAN AJAR MATA KULIAH FISIOLOGI REPRODUKSI DI JURUSAN BIOLOGI UNIVERSITAS NEGERI MALANG

Jurnal Florea Vol 2, No 2 (2015)
Publisher : Jurnal Florea

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Abstract

The purpose of the research and development is to produce teaching materials such as textbooks supporting course in Reproduction Physiology research based on the toxicity analysis for students whotake a course in Reproduction Physiology. This research is a research and development, where theresults of research become the teaching materials developed supporting Reproduction Physiologycourse. Development models have prepared using ADDIE Model. Sources of data in this researchinclude laboratory research and validation of the results sheet of Biotechnology material experts,instructional design experts, the media, and the trials in students. The analysis is used quantitative andqualitative.The results of the research have developed become teaching materials like text bookssupporting courses and have been validated by the Reproduction Physiology, the ReproductionPhysiology material experts, instructional design experts, the media, the individual testing, and grouptrials. Based on the results of the validation material have obtained value of 95%, the results of thedesign validation and media experts to get the value of 93.75%, it means that the teaching materials hasbeen developed which suitable to be used as a teaching material support biotechnology course. Whilethe experiments performed to get a data the percentage of students that are equal to 88,25 % andincluded in the category of “very good”, but still need to be revised based on advices and commentsfrom students.Keywords: Development, Instructional Material, ADDIE

PENGEMBANGAN BUKU AJAR PERKEMBANGAN HEWAN BERBASIS PENELITIAN METAMORFOSIS ULAT SUTERA BOMBYX MORI L.

Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan Vol.1, No.7, Juli 2016
Publisher : Graduate School of Universitas Negeri Malang

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Abstract

The developed teaching material in this study is in the form of a research-based textbook on silkworm Bombyx mori L hormone. It was aimed to develop the textbook on animal development course that will be used as one of supplementary learning textbook for students of S1 Biology Department of State University of Jember. The development of this textbook deployed the Hannafin and Peck developmental model which consists of three phases: (1) Needs Assessment (Analysis Purposes Phase), (2) Design (Design Phase), and (3) Developing/ Implementing (Development and Implementation Phases). It is a developmental model of instructional design which is oriented to products. The data were obtained starting from the needs analysis, product design, product manufacturing, and product validation. The instrument used to collect the research data development used a questionnaire and interview guidelines. These data were analyzed descriptively. The results of the analysis showed that this textbook has met the eligibility requirements. The feasibility of a limited test of individual students was 94.7% which was obtained with a valid category, the test feasibility of media experts obtained by 93.35% with a valid category, and the test feasibility of material presentation of 98.89% with a valid category and appropriate to be used.Bahan ajar yang dikembangkan berupa buku ajar berbasis penelitian hormon ulat sutera Bombyx mori L. Pengembangan bahan ajar yang dilakukan bertujuan untuk menghasilkan buku ajar matakuliah perkembangan hewan yang akan digunakan sebagai salah satu sumber buku penunjang belajar bagi mahasiswa Pendidikan Biologi S1 Universitas Negeri Jember. Pengembangan buku ajar ini menggunakan model pengembangan Hannafin dan Peck, yang terdiri atas 3 tahap yaitu (1) Need Assessment (Fase Analisis Keperluan), (2) Design (Fase Desain), dan (3) Develop/Implement (Fase Pengembangan dan Implementasi). Model pengembangan ini merupakan model desain pembelajaran yang berorientasi pada produk. Data diperoleh diawali dengan analisis kebutuhan, perancangan produk, pembuatan produk, dan validasi produk. Instrument yang digunakan untuk mengumpulkan data penelitian pengembangan menggunakan angket dan pedoman wawancara. Data dianalisis secara deskriptif. Berdasarkan hasil analisis menunjukkan buu ajar yang disusun telah memenuhi syarat kelayakan. Tingkat kelayakan uji terbatas perorangan mahasiswa diperoleh sebesar 94,7% dengan kategori valid, uji kelayakan dari ahli media diperoleh sebesar 93,35% dengan kategori valid, dan uji kelayakan sajian materi sebesar 98,89% dengan kategori valid dan layak untuk digunakan.

PRODUKSI ANTIBODI POLIKLONAL HASIL INDUKSI ISOLAT PROTEIN NON KINASE DARI MEMBRAN SPERMATOZOA MANUSIA

Indonesian Journal of Urology Vol 15, No 2 (2008)
Publisher : Indonesian Urological Association

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Abstract

Objective: The aim of this research is to get the profile of human sperms membrane protein based on identification for molecular weight, concentration protein, phosphorylation activity, and ability to raise enough antibodies. Material and methods: There are three phases in this research, first, isolation protein of human sperms membrane with using lysis buffer containing Tween-20, second, determination of molecular weight, phosphorylation activity, and protein concentration, and third, antibody production. Molecular weight of protein membrane was detected by SDS-PAGE, confirmation of protein concentration measured Biuret method, kinase activity measured using spectrophometry at its optimum condition, and then production of antibody that corfirmed by ELISA technique. Results: Human sperms contain the protein with molecular weight of 201, 163, 116, 97, 72, 56, 48, 32, 27, 20, and 17kDa. The highest phosphorylation activity was found on protein with molecular weight of 20 kDa is 30.197x 10-3 unit, while the lowest phosphorylation activity is 28.976x10-3 unit was shown on protein with 116 kDa of molecular weight and then   this protein can induce production of antibody. Biuret method show the protein concentration from protein with molecular weight 116 kDa is 410 ug/mL. Conclusion: The protein that has molecular weight of 116 kDa can be used as immunocontraception agent because phosphorylation activity has lower, good concentration, and could induce the production of antibody.

Pengembangan Buku Ajar Ekspresi Gen menggunakan Penelitian Deteksi dan Identifikasi Conception Rate Gene Sapi (Bos Taurus) Kembar Berbasis Mikrosatelit

Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan Vol 3, No 3: MARET 2018
Publisher : Graduate School of Universitas Negeri Malang

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Abstract

The needs analys to the students who take genetics course at the University of Jember shows that they are need textbooks based on research, especially gene expression material. The material has not been research-based and has not yet demanded students to utilize science and technology according to the KKKNI qualification framework. This study aims to develop textbooks of genetic subjects, especially gene expression material for undergraduate students, using the 4D development model. Validation and feasibility test data show that “Buku Ajar Ekspresi Gen menggunakan Penelitian Deteksi dan Identifikasi Conception Rate Gene Cow (Bos taurus) Kembar Berbasis Mikrosatelit” are very valid and very feasible so that it can be used by students of Jember University in genetics course.Analisis kebutuhan terhadap mahasiswa yang menempuh matakuliah genetika di Universitas Jember menunjukkan bahwa mahasiswa membutuhkan buku ajar berbasis riset materi ekspresi gen. Materi tersebut belum berbasis riset dan belum menuntut mahasiswa untuk memanfaatkan IPTEKS sesuai kerangka kualifikasi dalam KKNI. Penelitian ini bertujuan untuk mengembangkan buku ajar matakuliah genetika, khususnya materi ekspresi gen untuk mahasiswa S1, menggunakan model pengembangan 4D. Data hasil validasi dan uji kelayakan menunjukkan bahwa “Buku Ajar Ekspresi Gen menggunakan Penelitian Deteksi dan Identifikasi Conception Rate Gene Sapi (Bos taurus) Kembar Berbasis Mikrosatelit” sangat valid dan sangat layak sehingga dapat digunakan oleh mahasiswa Universitas Jember dalam matakuliah genetika.

HASIL VALIDASI BUKU TEKS MATAKULIAH BIOTEKNOLOGI BERBASIS BAHAN ALAM TANAMAN PACING (COSTUS SPECIOSUS SMITH) SEBAGAI ANTIFERTILITAS

Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan Vol.1, No.9, September 2016
Publisher : Graduate School of Universitas Negeri Malang

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Abstract

This research aimed to produce learning material in the form of Biotechnological subject support textbook based on research of techniques and steps to reveal Costus speciosus Smith. leaf extract potency to the mice spermatozoa motility and phosphorylation activity for university student taking Biotechnological subject. This research was developing research. The research and developing model used was ADDIE developing model. ADDIE model used five developing steps, included of analyze, design, development, implementation, and evaluation. The developing research data was textbook validity percentage from validation and opinion sheet obtained from tests result by material expert, media expert, and individual test by university student. The textbook validation result from test subject could be declared that it was very worthy and very valid so it was worthy to be used in Biotechnological subject.Penelitian ini bertujuan untuk menghasilkan bahan ajar berupa buku teks penunjang matakuliah Bioteknologi berbasis penelitian tentang teknik-teknik dan langkah-langkah untuk mengetahui potensi ekstrak daun pacing terhadap motilitas dan aktivitas fosforilasi pada spermatozoa tikus bagi mahasiswa yang menempuh matakuliah Bioteknologi. Penelitian ini adalah penelitian pengembangan. Model penelitian dan pengembangan yang digunakan yaitu model pengembangan ADDIE. Model ADDIE menggunakan lima tahap atau langkah pengembangan, yaitu analyze (analisis), design (desain), development (pengembangan), implementation (implementasi), evaluation (evaluasi). Data hasil penelitian pengembangan berupa persentase kevalidan buku teks dari lembar validasi dan tanggapan yang diperoleh dari hasil uji oleh ahli materi, ahli media, dan uji coba perorangan oleh mahasiswa. Hasil validasi buku teks dari subjek uji dinyatakan sangat layak dan sangat valid sehingga layak untuk digunakan pada matakuliah Bioteknologi.

PENGEMBANGAN MONOGRAF EKSPRESI MATERI GENETIK DENGAN MODEL THIAGARAJAN BERDASARKAN PENELITIAN TWINNING RATE GENE PADA SAPI KEMBAR

Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan Vol 2, No 11: November 2017
Publisher : Graduate School of Universitas Negeri Malang

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Abstract

The aim of this study was to produce monograph gene expression based on twinning rate gene research on twinning cow for Biology students university. The method of research and development adapted from Thiagarajan. The results of the assessment needed could be concluded that students supported to developed monograph about gene expression in genetic lecture. Validation from Subject expert and media were gift score 100% and 100%. The trial test result to a individual and medium group students was 84,91 % and 86,26%. The conclusion of this research and development was that monograph about gene expression in genetic lecture for Biology students in university has been produced with interpretation level was very good.Penelitian ini bertujuan untuk menghasilkan monograf ekspresi materi genetik berdasarkan penelitian gen kembar pada sapi untuk mahasiswa Biologi. Metodologi penelitian dan pengembangan ini mengadaptasi dari model Thiagarajan. Hasil dari analisis kebutuhan dapat disimpulkan bahwa dibutuhkannya monograf tentang ekspresi materi genetik di dalam perkuliahan. Validasi dari ahli materi dan media masing-masing mendapatkan skor 100 % dan 100 %. Uji coba perorangan dan kelompok sedang mendapatkan skor 84,91 % dan 86,26%. Kesimpulan dari penelitian dan pengembangan ini adalah telah dihasilkannya monograf ekspresi materi genetik untuk perkuliahan genetika pada mahasiswa Biologi dengan interpretasi sangat baik