Articles

Found 12 Documents
Search

EFEKTIVITAS PENEGAKAN SANKSI PIDANAUNDANG-UNDANG N0.23 TAHUN 2004TENTANG PENGHAPUSAN KEKERASAN DALAM RUMAH TANGGAUNTUK MELINDUNGIISTRI DARI KEKERASAN OLEH SUAMI Lestari, Sri Rahayu
YUSTISIA Vol 73 (2008)
Publisher : YUSTISIA

Show Abstract | Original Source | Check in Google Scholar

Abstract

The purpose ofthis research were toknow theeffectiveness ofenforcing criminal sanction as a view toUUNo.23 tahun 2004 about TheAbolition of Violence inthe Householdto give protection to wives from violence done byhusbands, andanyobstacles thatappeared in the effortofenforcing itscriminalsanction.Asaresearch with genderperspective, this research usedsociologicalornon positivistic approach supported byqualitative analysis. The respondents were wives who hadeverexperienced andbeen a victim of vio lenceactdonebytheirhusbands. The data gathered from therespondents which werefrom certain differ entareas;Kelurahan Jebres, Kelurahan Pucangsawit, andKelurahan Mojosongo. Besides that, therewas also the data, which was purposively taken from police offiers (RPK) in Poltabes Surakarta, KejaksaanNegeri Surakarta, andPengadilan Negeri Surakarta. Moreover, theinstruments of theresearch were in depth interviewanddocumentary research. While data analysis done byapplying deductive andinductive (mixed) wayof thinking which was known as theoretical interpretative. The result of theresearch showed that theenforcing ofcriminal sanction toward UUNo. 23 tahun 2004wasnoteffective ingiving protection to wives. In the reality, although there were lots ofhusbands who did violence acts(physic and mental) totheir wives, in fact itwas difficult toapply thecriminal sanction tothem. In other words, anycriminal sanctions that were gotten couldnotgiveprotection towives. Anyobstacles which were inhibited theeffectiveness of enforcing criminal sanction were: a. thecomplexity  andtheimplication ofthelowprocess before itwentto thecourt, b. thepaternalistic culture as an effect of feudalism, c. lawagencies who were notsensitive enough togenderissues, d. theinexistence ofevidence instruments, in this case visum etrepertum, and e. the womens lack ofunderstanding towardUUPKDRT.
KAJIAN NUTRIGENOMIK: PENGHAMBATAN Igf-1 PADA ADIPOGENESIS JARINGAN LEMAK VISERAL TIKUS DENGAN EKSTRAK KULIT RAMBUTAN Nurdiansyah, Rizky; Lestari, Sri Rahayu; Fatchiyah, Fatchiyah
Biotropika Vol 1, No 6 (2013): Biotropika
Publisher : Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Original Source | Check in Google Scholar

Abstract

Tujuan penelitian adalah untuk mengetahui dan menjelaskan pengaruh dari ektrak kulit buah rambutan terhadap adipogenesis jaringan lemak viserall tikus dengan melihat ekspresi igf-1. Tahapan penelitian yang dilakukan adalah ekstraksi kulit buah rambutan, perlakuan pada tikus, isolasi protein lemak viserall, separasi protein dan western blot. Tikus strain wistar jantan dibagi menjadi tikus berat badan normal dan obesitas. Perlakuan yang dilakukan antara lain kontrol, asam elagat, placebo, dan perlakuan ekstrak dengan dosis 5, 10, 15, dan 20 mg/kg berat badan. Hasil menunjukkan bahwa tren penurunan berat badan terjadi pada grup tikus obesitas dengan asupan pakan yang tidak berbeda signifikan (P>0,05). Dosis efektif terlihat pada perlakuan 10 mg/kg dan pada dosis 20mg/kg justru terjadi kenaikan berat badan untuk kedua jenis grup tikus. Profil protein antara tikus normal dan obesitas menunjukkan pola yang hampir sama dengan perbedaan intensitas tiap pita. Ekspresi igf-1 terpaut dengan ekspresi igfbp-1 pada 36,7 kDa dan terjadi pada seluruh perlakuan dengan perbedaan nyata pada kedua grup. Ekstrak kulit buah rambutan dengan dosis efektif 10mg/kg memiliki efek anti-obesitas pada tikus obesitas. Menariknya, ekspresi igf-1 menurun pada perlakuan 20mg/kg berat badan dibandingkan dengan kelompok lain. Hal ini diduga penghambatan obesitas tidak melewati jalur tyrosin kinase pada IR family.
PENGARUH DEWAN DIREKSI, KOMISARIS INDEPENDEN, KEPEMILIKAN MANAJERIAL DAN KEPEMILIKAN INSTITUSIONAL TERHADAP KINERJA PERUSAHAAN DENGAN ENTERPRISE RISK MANAGEMENT SEBAGAI INTERVENING (Studi Empiris pada Perusahaan Manufaktur yang Terdaftar di Bursa Efek Indonesia Periode Tahun 2012-2014) Lestari, Sri Rahayu; Sari, Ria Nelly; A, Al Azhar
Jurnal Online Mahasiswa (JOM) Bidang Ilmu Ekonomi Vol 4, No 1 (2017): Wisuda Februari
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Ilmu Ekonomi

Show Abstract | Original Source | Check in Google Scholar

Abstract

The purpose of this study is to examine the effect of Good Corpotare Governance’s mechanism that consist of Board of Directors, Independent Commissioner, Manajerial Ownership, and Institusional Ownership on the existence of Enterprise Risk Management and the effect of the Enterprise Risk Management on firm performance. In addition, this study also examines the intervening role of Enterprise Risk Management on the relationship between Corporate Governance and firm performance. The population of this study was manufacturing companies listed in Indonesia Stock Exchange for the 2012 to 2014 financial year. By using purposive sampling method, 25 firms were selected as a sample. This study used data from company’s annual report. The analysis technique used multiple linear regression analysis using SPSS computer program. The results of analysis showed that Board of Directors, Independent Commissioner, Manajerial Ownership, and Institusional Ownership affect the existence of Enterprise Risk Management and the existence Enterprise Risk Management affects firm performance. The result also proved that Enterprise Risk Management act as an intervening variable among Board of Directors, Independent Commissioner, Manajerial Ownership, and Institusional Ownership on firm performance.Keyword : Good Corporate Governance, Enterprise Risk Management and Firm Performance
PENGARUH EKSTRAK KULIT BUAH RAMBUTAN (Nephelium lappaceul L.) TERHADAP NEKROSIS SEL HEPAR TIKUS (Ratus novergicus) OBESITAS Mahmudah, Anisah; Tenzer, Amy; Lestari, Sri Rahayu
Bioeksperimen: Jurnal Penelitian Biologi Vol 4, No 1: Maret 2018
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Original Source | Check in Google Scholar | Full PDF (210.115 KB)

Abstract

Obesitas saat ini disebut sebagai “sindrom baru dunia” yang memerlukan terapi untuk mengatasinya. Terapi herbal adalah terapi yang paling banyak dilakukan penderita obesitas. Kulit buah rambutan mengandung polifenol yangmempunyai aktivitas antioksidan dan berpotensi untuk dikembangkan sebagai obat terapi obesitas. Hepar dapat terkena efek samping obat karena bertanggung jawab melakukan metabolisme obat, termasuk karena pemberian ekstrak kulit buah rambutan. Penelitian ini bertujuan mengetahui pengaruh pemberian ekstrak kulit rambutan (Nephelium lappaceum L.) terhadap nekrosis sel hepar tikus (Ratus norvegicus) obesitas. Penelitian ini merupakan penelitian eksperimental rancangan acak lengkap (RAL) dengan 4 perlakuan. Dua puluh empat tikus jantan strain wistar umur 14 minggu yang mengalami obesitas dikelompokkan secara acak menjadi 4 kelompok, diberi ekstrak kulit buah rambutan melalui sonde lambung dengan dosis 15, 30, dan 60 mg/kgbb dan kelompok kontrol diberi akuades. Setelah 12 minggu, tikus didekapitasi dan dikoleksi organ heparnya untuk dibuatpreparat histologi dengan pewarnaan Hemotoksilin-Eosin (HE). Tiap sayatan diamati dalam 3 lapang pandang, diamati 100 sel hepatosit, dihitung persentase sel yang mengalami nekrosis menggunakan hand tally counter. Analisisdata menggunakan analisis varian tunggal pada taraf kepercayaan 95%, dilanjutkan dengan uji BNT dengan taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa ekstrak kulit buah rambutan yang berpengaruh signifian terhadap nekrosis sel hepar tikus adalah 60 mg/kgbb, yaitu meningkatkan persentase piknosis, karyoreksis, karyolisis, serta total nekrosis
POTENSI BUAH MERAH (Pandanus conoideus Lam.) DALAM MENURUNKAN KADAR LDL DARAH TIKUS PUTIH Agnesa, Oki Sandra; Waluyo, Joko; Prihatin, Jekti; Lestari, Sri Rahayu
Bioeksperimen: Jurnal Penelitian Biologi Vol 3, No 1: Maret 2017
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Original Source | Check in Google Scholar | Full PDF (364.14 KB)

Abstract

Penyakit jantung koroner (PJK) terutama disebabkan oleh aterosklerosis karena hiperkolesterolimia. Oksidasi low density lipoprotein (LDL) oleh reactive oxygen species (ROS) sebagai penyebab utama proses aterogenik dapat dicegah dengan kehadiran antioksidan seperti vitamin E. Buah dan sayuran banyak mengandung vitamin. Salah satu buah yang mengandung vitamin E adalah buah merah (Pandanus conoideus Lam.). Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak buah merah terhadap kadar LDL darah pada tikus putih (Rattus norvegicus L.). Jenis penelitian ini adalah penelitian eksperimental laboratorium yang didesain mengikuti Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 4 kali ulangan dengan parameter penelitian adalah kadar LDL darah tikus putih. Data dianalisis menggunakan one way anova dilanjutkan dengan uji Duncan 95%. Berdasarkan hasil penelitian, minyak buah merah memiliki kemampuan untuk menurunkan kadar LDL darah tikus putih.
The Potential of Single Garlic Oil in Inhibiting The Growth and Damaging The Membrane of Pseudomonas aeruginosa Bacteria Lestari, Sri Rahayu; Witjoro, Agung; Poejiani, Soeyati
Journal of Tropical Biodiversity and Biotechnology Vol 3, No 3 (2018): December
Publisher : Universitas Gadjah Mada

Show Abstract | Original Source | Check in Google Scholar | Full PDF (832.257 KB)

Abstract

Nosocomial infection caused by Pseudomonas aeruginosa bacteria is hard to be treated since the infection transmission is fast and it is resistance to the antibiotic. Antibiotic resistance in the bacteria can be treated with various natural ingredients from plants and one of the plants is single garlic. Single garlic contains organosulfur compounds in form of alliin, allicin, and ajoene. This research aimed to analyze the potential of single garlic oil (SGO) in inhibiting the growth and the damage of membrane of P. aeruginosa bacteria in in vitro with disc diffusion method by giving treatments of essential oil extract in concentration of 25 mg/ml, 50 mg/ml, 75 mg/ml and 100 mg/ml, and 1% dimethylsulfoxide (DMSO) as the negative control and Ceftazidimeof 30 µg/ml as the positive control. The damage of the bacterial cell membrane was observed with Scanning Electron Microscopy (SEM) with a magnification of 25000X. The result of the measurement of the zone of inhibition was analyzed using one-way ANOVA. The research results indicate that SGO could inhibit the growth of P. aeruginosa bacteria with strong criteria, which was in a concentration of 100 mg/ml.
The effect of polyphenol to visceral fat profile protein at obesity model rat Lestari, Sri Rahayu; Wulandari, Nuning; Maslikah, Siti Imroatul
Biomedical Engineering Vol 2, No 1 (2016)
Publisher : Biomedical Engineering

Show Abstract | Original Source | Check in Google Scholar | Full PDF (413.463 KB)

Abstract

Objective: To asses visceral fat profile protein between normal and obesity model rat. Material and Methods: Normal and obesity rat model have been treated with polyphenol from rambutan peel extract for 12 weeks. Rat were divided into 2 major group  based on their weight which normal rat and obesity model rat respectively have average weight 180-200 gram and 360-380 gram. This treatment were divided into minor group which are placebo (without treatment),  treatment with ellagic acid and treatment with polyphenol (dosage 15 mg/kg body weight; 30 mg/kg body weight and 60 mg/kg body weight. Rat were sacrificed after 12 weeks treatment to asses visceral fat profile protein and continue with running SDS PAGE12,5 %. Protein molecular weight sample were calculated with regression analysis between marker protein mobility and logarithm from marker. Band protein analysis were analyze qualitative with SDS PAGE meanwhile quantitative analysis with Gel Doc (Bio Rad). Density band protein were analyze were using Quantity One software and confirmed with Western Blotting.Result: Protein profile characteristic normal rat and obesity-model rat were in range between117-20 k Da. The amount of band protein were found in normal rat were less than the amount of protein at obesity-model rat. Conclusion: There were difference molecular weight at protein density 57 k Da for obesity model rat which has been treated with rambutan peel extract.
Senyawa Alami Bawang Putih Tunggal sebagai Inhibitor LpxC Bakteri Pseudomonas aeruginosa melalui Virtual Screening Fitriana, Nur; Lestari, Sri Rahayu; Lukiati, Betty
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 18 No 1: January 2018
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Original Source | Check in Google Scholar | Full PDF (1028.778 KB)

Abstract

Pseudomonas aeruginosa merupakan salah satu bakteri Gram negatif penyebab infeksi nosokomial yang memiliki resistensi tinggi terhadap berbagai antibiotik karena adanya lipid A, komponen LPS bersifat toksik pada inang. Sintesis lipid A dimulai dari Kdo2 -lipid A, difasilitasi oleh enzim LpxC. Enzim LpxC merupakan target pengembangan antibiotik karena adanya kofaktor Zn2+. Bawang putih tunggal merupakan herbal potensial pengganti antibiotik sintetis. Alliin, Allicin dan Ajoene yang terkandung dalam bawang putih tunggal memiliki aktivitas antibakteri. Penelitian ini bertujuan memprediksi potensi senyawa organosulfur bawang putih tunggal sebagai agen antibakteri melalui virtual screening menggunakan metode molecular docking. Metode yang dilakukan adalah memprediksi potensi senyawa organosulfur menggunakan web server PASS dan melakukan docking untuk mengetahui interaksi ligan-protein target. Hasil penelitian menunjukkan, senyawa organosulfur berpotensi sebagai antibakteri, antibiotik, dan immunomodulator. Aktivitas antibakteri terbaik ditunjukkan oleh senyawa Alliin dengan nilai afinitas pengikatan -5,2 kkal/mol. Semua senyawa organosulfur memiliki sisi aktif yang sama dengan kontrol (Ciprofloxacin dan Imipenem) pada enzim LpxC. Sisi aktif ditunjukkan dengan adanya residu asam amino (Ser295, Val182, dan Tyr 296) yang berikatan dengan ikatan hidrogen dan interaksi hidrofobik (Phe152, Phe181, Gly85, Phe176, Ala84, Val182, Ser180, Ile241, Leu86, Tyr296, Ser295 dan Met297) antara ligan-protein target.
GAMBARAN HEMATOLOGI MENCIT (Mus musculus) MODEL TOKSISITAS SUBKRONIS Pujiastuti, Ita Nur Eka; Lestari, Sri Rahayu; Gofur, Abdul
Scripta Biologica Vol 4, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Original Source | Check in Google Scholar | Full PDF (816.494 KB)

Abstract

Garlic commonly is consumed as medicine to prevent or heal illness or to maintain someone's health. Many societies prefer garlic (Allium sativum) among other herbal remedies for cholesterol treatment. It consists of several types, and one of them is single bulb garlic used to treat hypertension. There has been, however, no published research reporting the toxicological properties of single bulb garlic. The purpose of this study was to determine subchronic toxic effects of single bulb garlic administered to mice using hematological parameters. The experiment parameters were hemoglobin and hematocrit levels, the number of erythrocytes and leukocytes. Male mice (Mus musculus) strain Balb-C were treated with single bulb garlic extract for 28 days with dosage levels of 0% (N ), 0.25% (P1 ), 0.5% (P2 ), 1% (P3 ), and 2% (P4 ). Single bulb garlic showed no effect on hemoglobin and hematocrit levels but increased the number of erythrocyte and leucocyte. We concluded that single bulb garlic did not cause subchronic toxic effects.
Single Clove Garlic (Allium sativum) Essential Oil as an Inhibitor of Staphylococcus aureus Bacteria Gofur, Abdul; Wulandari, Ida; Athoillah, M. Fitri; Witjoro, Agung; Lestari, Sri Rahayu
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Original Source | Check in Google Scholar | Full PDF (312.475 KB)

Abstract

Staphylococcus aureus is gram-positive bacteria that often infect the skin. S. aureus has been experiencing resistance to several antibiotics. One of the solutions to overcome the resistance is by using garlic that is believed by the society can overcome bacterial infection. The study aimed to find out the influence of the variation in concentration of local single clove garlic essential oil used on the inhibition and damage of morphological structure of S. aureus bacteria. The inhibition test was conducted using disc diffusion method. The experiment groups consisted of  1% DMSO as negative control, vancomycin 30µg/ml as positive control and single clove garlic essential oil (25 mg/ml, 50 mg/ml, 75 mg/ml and100 mg/ml) as treatment groups. The diameter of inhibition zone was measured using calipers. The morphological damage of the bacterial cells can be seen using Scanning Electron Microscopy (SEM) with magnification of 25000x. The result of Kruskal-Wallis test analysis indicated that the extract of local garlic essential oil has inhibitory activities against S. aureus bacteria (P<0.05). The damage to the morphological structure of bacterial cells with the administration of 30 µg/ml vancomycin was equal to 100 mg/ml single clove garlic essential oil extract. Single clove garlic essential oil can be used as an alternative treatment for skin infection diseases by inhibiting S. aureus growth.