Sartje Lantu
Aquaculture Program, Faculty of Fisheries and Marine Science, Sam Ratulangi University, Manado

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Purification of carp (Cyprinus carpio) kidney cathepsin C Pangkey, Henneke; Lantu, Sartje
e-Journal BUDIDAYA PERAIRAN Vol 1, No 1 (2013): Vol.1 No. 1 Januari 2013
Publisher : e-Journal BUDIDAYA PERAIRAN

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Abstract

Pemurnian enzim cathepsin C diperoleh melalui penggunaan colom kromatografi S-Sepharose FF, Sephacryl S-200 HR, Concanavalin A-Agarose, Affi-gel 501 dan DEAE-Sephacel. Dari 160 g ginjal ikan mas diperoleh 0,33 mg enzim cathepsin C, dengan hasil pemurnian 150 kali. Berat molekul cathepsin C adalah 170 kDa yang terdiri dari 5 subunit, dengan berat molekul berkisar antara 10 sampai 20 kDa. Cathepsin C adalah oligomerik protein dan aktif sebagai eksopeptidase (pH 5) maupun endopeptidase (pH 7)
OSMOREGULASI PADA HEWAN AKUATIK Lantu, Sartje
JURNAL PERIKANAN DAN KELAUTAN TROPIS Vol 6, No 1 (2010)
Publisher : Sam Ratulangi University

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Abstract

All aquatic organisms must deal with the problem of maintaining internal homeostasis, a constant internal chemical environment in which that organisms enzymes can operate efficiently.  As we saw earlier, this is largely a matter of osmoregulation, but other factors are involved also.  Many marine organisms can get by with minimal osmoregulation since the oceans, where the enzymes evolved, are already a good environment; other marine organisms maintain their body fluids at ionic concentrations different from the surrounding ocean and must actively regulate ions.  Freshwater also calls for active measures to maintain proper osmotic balance.
PROSPECT OF SEA CUCUMBER CULTURE IN INDONESIA AS POTENTIAL FOOD SOURCES Pangkey, Henneke; Lantu, Sartje; Manuand, Lusia; Mokolensang, JeffrieFredrik
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 2 (2012): Volume 15, Number 2, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Sea cucumber is one of the sea treasures which has been used not only as luxury food for certain countries but also as medicines. Sea cucumber has become one of the most important products and it has high price in international market.For this reason, the exploitation of sea cucumber turns out to be excessive and disturbs its sustainability. Aquaculture is the best way to prevent this problem. The success of sea cucumber culture is very depend on seed availability and suitable food for larvae as well as juvenile along with growing out stadia for market requirement.
Pertumbuhan Cladocera jenis Chydoridae pada media kultur yang berbeda Sambode, Denovis; Pangkey, Henneke; Lantu, Sartje
e-Journal BUDIDAYA PERAIRAN Vol 1, No 2 (2013)
Publisher : e-Journal BUDIDAYA PERAIRAN

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The aim of this study was to find out the effect of different organic fertilizers on the population growth of Chydorus sp. This research was conducted at Laboratorium of Nutrition and Food Technology, Faculty of Fishery and Marine Sciences, Sam Ratulangi University, from December, 2012 to January, 2013. The experiment was run in the Completely Randomized Design with 3 treatments and 3 replication. Chydorus sp. was cultured in 9 glass jars with a volume of 1 L each. Three treatments applied in this research included A: medium culture composed of 500ml of water and 50 grams of soil; B: medium culture composed of 500ml of water, 50 grams of soil and 10g of cow dung; C: medium culture composed of 500ml of water, 50 grams of soil and 10g of horse dung. The density of Cydorus sp. in each media was 10 individuals /500 ml water. Observation on the population growth, development of live preys, and water quality parameters was conducted for 20 days period. Data were statistically analized with Analysis of variance. The results showed the highest density of Chydorus sp. was reached by Chydorus cultured in medium with horse manure (2169 individual/500 ml), followed by medium culture with cow manure (1715 individual/500 ml), and the lowest in medium culture with soil which was 1065,33 individual/500 ml. However, Analysis of variances showed that Chydorus population growth were not significantly affected by different culture medium. Keywords: manure, liquid fertilizer, growth, Chydorus sp.
Ratio of C:N in culture media of silk worm, Tubifex sp. Solang, Jhonly; Pangkey, Henneke; Wullur, Stenly; Lantu, Sartje
AQUATIC SCIENCE & MANAGEMENT (Jurnal Ilmu dan Manajemen Perairan) Vol 2, No 1 (2014): April
Publisher : Master Program of Aquatic Sciences

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Abstract

Title (Bahasa Indonesia): Rasio C:N pada media kultur cacing sutra, Tubifex sp. This study aimed to determine the C:N ratio on each medium for the growth of the sludge worm. This study used mud and soybean curd residue (treatment A), mud and chicken manure (treatment B), mud and pig manure (treatment C), and control (mud only) (K) as culture media of the sludge worm (Tubifex sp.). The decomposition process was proceeding for six days, and then the sludge worms were stocked with initial weight of 30 grams per container. Culture period was 21 days in running water systems. The resulting C:N ratio was 60.5 for treatment A, 45.8 for treatment B, 36 for treatment C and 35 for K. The soybean curd residue and mud medium gave the highest influence on the growth of the sludge worm, followed by pig manure and mud, chicken manure and mud, and then mud (control). Penelitian ini bertujuan untuk menentukan perbandingan C:N ratio dalam media budidaya untuk pertumbuhan cacing sutra. Penelitian ini menggunakan lumpur dan ampas tahu (perlakuan A), lumpur dan kotoran ayam (perlakuan B), serta lumpur dan kotoran babi (perlakuan C) dan perlakuan K (kontrol: hanya lumpur) sebagai media kultur cacing sutra (Tubifex sp). Proses dekomposisi dilakukan selama 6 hari, kemudian dilakukan penebaran cacing sutra dengan berat awal 30 gram/wadah penelitian. Waktu pemeliharaan dilakukan selama 21 hari dalam sistem air mengalir. Hasil penelitian menunjukkan rasio C:N sebesar 60,55 untuk perlakuan A, 45,85 untuk perlakuan B, 36,08 untuk perlakuan C, dan 35,25 untuk K. Media yang menggunakan ampas tahu dan lumpur memberikan pengaruh tertinggi terhadap pertumbuhan cacing sutra, kemudian disusul oleh media kotoran babi dan lumpur, media kotoran ayam dan lumpur dan terendah media lumpur (kontrol).
Pengaruh pemberian beberapa jenis pakan pada pertumbuhan ikan kerapu Cantang Epinephelus sp di Karamba Jaring Apung di Teluk Talengen Kepulauan Sangihe Tumadang, Liswara S.N.; Sampekalo, Julius; Lantu, Sartje
e-Journal BUDIDAYA PERAIRAN Vol 4, No 3 (2016)
Publisher : e-Journal BUDIDAYA PERAIRAN

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Abstract

The research was conducted in floating net cage for at Talengen Island Sangihe to study the growth of grouper (46-70 g in average) fed various type of feed including fish flesh, pellet, cassava, sago) for six weeks, and to determine the food conversion ratio of each food type. The treatments consisted of A (fish flesh), B (fish flesh + cassava), C (fish flesh+sago), D (pellet). Fish was culture in 12 floating net cages measuring 1x1x1,5 m each, 10 fish/cage. Research results showed that the highest weight gain was achieved in fish in treatment C (87.6%), followed by treatment A (76.1%) , B (69.8%), and D (52.5%).  Statistically, treatment C was significantly different  compared to treatment A, B and D. The use of fish flesh combined with sago was able to improve growth of grouper Kata kunci : Kerapu Cantang,  growth, food conversion ratio, floating net cage
Iptek bagi Masyarakat (IbM) Kelompok Petani Ikan Kelurahan Rap Rap Kalesaran, Ockstan J.; Lantu, Sartje
e-Journal BUDIDAYA PERAIRAN Vol 4, No 3 (2016)
Publisher : e-Journal BUDIDAYA PERAIRAN

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Abstract

The implementation of science and technology for society aimed to give knowledge and methods of application that can answer all problems encountered by fish farmers. Low cost of  feed manufacturing methods and high quality utilizing local raw ingredients had been given through counseling and basic training so that fish farmers can continue going forward and do not rely on commercial feed from the market.  Practical sex reversal methods were given, intended that fish farmers could get male tilapia seeds having rapid growth.   Keywords: Fish farmer, Rap Rap Village, feed, eeed, Sex Reversal Methods
Budidaya Maggot (Hermetia illuens) dengan menggunakan beberapa media Mudeng, Nico E.G.; Mokolensang, Jeffrie F.; Kalesaran, Ockstan J.; Pangkey, Henneke; Lantu, Sartje
e-Journal BUDIDAYA PERAIRAN Vol 6, No 3
Publisher : e-Journal BUDIDAYA PERAIRAN

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Abstract

The purpose of this study was to determine the best culture medium for producing maggot (Hermetia illucens) and to determine the production quantity in each different medium.  This research was controlled two times a day at 09:00 a.m. and 16:00 p.m. Cultivation media were prepared from coconut pulp, tofu pulp, rice bran and restaurant wastes. Each medium was weighed as much as one kg and then placed into a culture container. The research used Completely Randomized Design consisting of 4 treatments, each with three replications. The parameters observed consisted of temperature and pH of the media  measured at 07.00 am and 17.00 pm, and maggot production measured on the last day. During the ten days of experiment, the production of maggot in the treatment using restaurant waste media was significantly different as compared to other treatments. This study found that a good medium for the production of maggot was prepared using restaurant waste. Keywords: Hermetia illucens, coconut pulp, restaurant waste
Pemanfaatan bungkil kelapa fermentasi dalam pada ikan nila (Oreochromis niloticus) Horopu, Meyriska C.; Sampekalo, Julius; Lantu, Sartje
e-Journal BUDIDAYA PERAIRAN Vol 6, No 3
Publisher : e-Journal BUDIDAYA PERAIRAN

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Abstract

The purpose of this study was to determine the concentration of the use of the best fermented coconut pulp in feed on the growth of tilapia.  The research  used 12  nets  measuring 1x1 m placed in a concrete pond measuring 3.5 x 5 cm. The treatments were A (without fermented coconut pulp), B (10% fermented coconut pulp), C (20% fermented coconut pulp), and D (30% fermented coconut pulp). The experimental method used was a completely randomized design with 4 treatments and 3 repetitions. Data collected were absolute growth, daily growth, relative growth, and feed efficiency. The results of the variance analysis showed an influence in the growth of tilapia size 5-8 cm. The best growth and feed efficiency value occurred in treatment D Thus  the use of  30% fermented coconut pulp in fish food could improve the growth of tilapia.Key words : fermented coconut pulp, tilapia, growth feed efficiency
Utilization of life feed Alona sp., Boiled Egg Yolk and Commercial Feed Toward Survival Rate of Betta Fish Larvae David, Julio; Lantu, Sartje; Pangkey, Henneke D.; Lumingas, Lawrence J.L.; Mingkid, Winda M.; Bataragoa, Nego E.
JURNAL ILMIAH PLATAX Vol 7, No 2 (2019): EDISI JULI - DESEMBER 2019
Publisher : Sam Ratulangi University

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The purpose of this study was to find out the survival rates of betta fish larvae (cupang) that were given life feed Alona sp., boiled egg yolk and commercial feed for 21 days. Data analysis was performed by one-way ANOVA, and continued with BNJ (Tukey) test. The result of the survival rate is as follow treatment with life feed Alona sp. 56%, treatment with boiled egg yolk 26%, and treatment with commercial feed 0%. The results of statistical tests show there are significant differences.  Significant value p <.0002 (<.05). Water quality parameter measured during the study was temperature of 26 ºC.Keywords: betta fish larvae, life feed, Alona sp., survival rateABSTRAKTujuan penelitian ini adalah untuk mendapatkan laju kelangsungan hidup larva ikan betta (cupang) yang dibeerikan pakan alami Alona sp., rebusan kuning telur dan pakan komersil selama 21 hari.  Analisis data dilakukan dengan ANOVA one way, dan dilanjutkan dengan uji BNJ (Tukey).  Hasil laju kelangsungan hidup adalah sebagai berikut, perlakuan dengan pakan alami Alona sp. 56%, perlakuan dengan rebusan kuning telur 26%, dan perlakuan dengan pakan komersil 0%.  Hasil uji statistik menunjukan adanya perbedaan yang nyata.  Nilai signifikan p <.0002 (<.05).  Parameter kualitas air yang diukur selama penelitian adalah suhu 26ºC.Keywords: larva ikan betta, pakan alami, Alona sp., kelangsungan hidup