Asmarani Kusumawati
Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Published : 44 Documents
Articles

KLONING DAN EKSPRESI GEN PENYANDI SAGI TOXOPLASMA GONDII ISOLAT LOKAL PADA VEKTOR pGEX-2T Hartati, Sri; Wuryastuti, Hastari; Widada, Joannes Sri; ., Sumartono; Kusumawati, Asmarani
Jurnal Sain Veteriner Vol 22, No 2 (2004)
Publisher : Fakultas Kedokteran Hewan

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CORRELATION OF PROGESTERONE AND CORTISOL PLASMA LEVELS BETWEEN OVULATED AND NON-OVULATED ETTAWA CROSSBREDS DOES Astuti, Pudji; Tri Widayati, Diah; -, Sunendar; Suharto, Kresno; Kusumawati, Asmarani; Junaidi, Aris
Jurnal Veteriner Vol 9, No 1 (2008)
Publisher : Jurnal Veteriner

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This experiment was conducted to determine the correlation of progesterone and cortisol levels in plasma between ovulated and non-ovulated Crossbreds Ettawa does. Eight does were used in this experiment and they were divided equally into 2 groups, i.e. group I consisting of 4 normal ovulated does with the average body weight of 37.5 ± 3.109 kg and group II consisting of 4 non-ovulated does with the average body weight of 28 ± 2.160 kg as group II. The estrus cycles of all does were synchronized using intravaginal device of controlled internal drug release (CIDR) accompanied by intra-muscular injection of prostaglandin F2 (PGF2) alpha. Immediately after the onset of estrus, blood samples were collected from jugular every 3 and 6 hours for 72 hours after onset of estrus. The concentration of cortisol and progesterone in plasma was assayed by enzyme linked immunosorbent assay (ELISA). The results showed that the average concentrations of cortisol in ovulated does (90.89±26.22 ng/mL) was higher than in non-ovulated does (42.70±37.18 ng/mL). Similarly, the concentrations of progesterone in ovulated does (0.098±0.0423 ng/mL) was higher than in non-ovulated does (0.093±0.056 ng/mL). It was evident that the change in progesterone level was closely associated with the change in cortisol level in plasma.
Physiological Response of Bligon Buck to Transportation: Relation to Level of Thyroid Hormone Astuti, Pudji; -, Sarmin; Kusumawati, Asmarani; Mona Airin, Claude; Maheshwari, Hera; Sjahfirdi, Luthfiralda
Jurnal Veteriner Vol 11, No 2 (2010)
Publisher : Jurnal Veteriner

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Transportated animals may subject to a variety of physical stimuli including metabolism, crowding,noise, handling, isolation, agitation, and extreme temperature .The aim of this study was to determine thechanges of serum T4 and T3 concentration, during animals transportation. Six adult Bligon buck with bodyweight ranging from 26-30 Kg were used in this study. Two weeks prior to the experiment, the animalswere given anthelmintic Albendazole to eliminate egg worm. All animals were fed standard diet in theirpen at 10% of their body weight per head daily and commercial concentrate also given everyday. Freshwater was provided ad libitum. All animals were transported around village for 16 hours starting from18.00 pm until 10.00 am in open small truck (3 x 2 m); eye contact each others would be possible. Bloodsamples were withdrawn from jugular vein using vacutainer tubes containing heparin into 1.5 mL glasstubes, then centrifuged at 500 g for 15 minutes. Plasma was collected to be stored at –20° C. The bloodwere collected every 4 hours from 8 hours before transportation ( at 10.00 am, 14.00 pm and 18.00 pm)until the time of arriving after transportation at 10.00 am. Plasma was harvested and stored at –20° Cuntil T3 and T4 concentrations were measured using ELISA method (enzyme linked immunosorbentassay) product DRG, Germany. The result showed that transportation of Bligon bucks for 16 hours havean affect on level of T4 only (P<0.05) and not for T3 concentration (P>0.05) due to physical stimuli such ascrowding, heat stress, noise, handling would be discarded so that the metabolic process was stable. Duringtransportation, decreasing of T4 levels indicated conversion of T4 to T3 to form active hormone.
Isolation-Amplification of ENV-TM Subunit Genes of Jembrana Disease Virus by a Single Step RT-PCR and Its Direct Cloning in PCR2.1-TOPO Plasmid Kusumawati, Asmarani; Hidayat, Basuki; Sardjono, B.; Widada, Joannes Sri
Buletin Peternakan Vol 27, No 1 (2003): Buletin Peternakan Vol. 27 (1) Februari 2003
Publisher : Fakultas Peternakan UGM

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Penggunaan Protein Reporter GFP (Green Fluorecent Protein) untuk Pemantauan Fungsi Annexin I pada Phagositosis Brucellasuis Kusumawati, Asmarani
Jurnal Sain Veteriner Vol 20, No 2 (2002)
Publisher : Fakultas Kedokteran Hewan

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PENGARUH PEMBERIAN DEKSAMETASON PADA KELINCI LOKAL BUNTING YANG DIFETEKTOMI Soebagyo, Slamet; Kusumawati, Asmarani
Jurnal Sain Veteriner Vol 21, No 2 (2003)
Publisher : Fakultas Kedokteran Hewan

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CLONING OF THE GENE ENCODING SAGI OF LOCAL ISOLATE TOXOPLASMA GONDII IN ESCHERICHIA COLI DH5a Hartati, Sri; Widada, Sri; ., Sumartono; Kusumawati, Asmarani
Jurnal Sain Veteriner Vol 21, No 2 (2003)
Publisher : Fakultas Kedokteran Hewan

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Faktor Resiko Bovine Brucellosis pada Tingkat Peternakan di Kabupaten Belu Propinsi Nusa Tenggara Timur Maria Tae Lake, Rosalinda Petra; Kusumawati, Asmarani; Budiharta, Setyawan
Jurnal Sain Veteriner Vol 28, No 1 (2010)
Publisher : Fakultas Kedokteran Hewan

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ISOLATION AND CLONING OF ENV-TM SUBUNIT GENE OF JEMBRANA DISEASE VIRUS IN THE PROKARYOTIC EXPRESSION VECTOR pGEX-2T Kusumawati, Asmarani; Martien, Ronny; Mangkoewidjojo, Soesanto; Widada, Joeannes Sri
Jurnal Sain Veteriner Vol 21, No 2 (2003)
Publisher : Fakultas Kedokteran Hewan

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CONSTRUCTION OF AN EXPRESSION VECTOR BASED ON pCDNA3.1(+) EXPRESSING JEMBRANA DISEASE VRIUS ENV-TM SUBUNIT GENE IN EUKARYOTIC SYSTEM Kusumawati, Asmarani; Widada, Joannes Sri; Hidayat, Basuki
Jurnal Sain Veteriner Vol 22, No 1 (2004)
Publisher : Fakultas Kedokteran Hewan

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