Hermin Pancasakti Kusumaningrum
Biology Department, Faculty of Sciences and Mathematics, Diponegoro University

Published : 43 Documents
Articles

Konsumsi Harian Copepoda terhadap Pakan Chlorella sp. pada Volume Media Kultivasi yang Berbeda Zaenuri, Muhammad; Endrawati, Hadi; Kusdiyantini, Endang; Kusumaningrum, Hermin Pancasakti
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 13, No 3 (2008): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Penelitian ini bertujuan untuk mengetahui konsumsi harian copepoda terhadap Chlorella sp. pada volume media kultivasi yang berbeda. Penelitian ini dilaksanakan dari Mei hingga Oktober 2005 di Laboratorium Biologi Oseanografi UNDIP. Copepoda diambil per bulan dari bulan Mei hingga Oktober 2005 di Perairan Demak. Chlorella sp. digunakan sebagai pakan copepoda, berasal dari Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara, dengan densitas 15.200 sel/mL. Kultivasi copepoda menggunakan 3 beker 2 L dengan densitas awal 100 ind./L dan 3 akuarium 20 L dengan densitas awal 1000 ind./L. Pengamatan konsumsi harian copepoda terhadap Chlorella sp. dilakukan per 3 jam dari pukul 06.00 sampai 18.00. Perioda kultivasi diulang per bulan selama 6 bulan. Parameter suhu, salinitas, DO dan pH diamati setiap hari. Konsumsi rata-rata copepoda terhadap Chlorella sp. menunjukkan 19,05- 140,47 sel/mL/jam pada media 2 L dan 10,69—102,06 sel/mL/jam pada media 20 L. Model konsumsi copepoda terhadap Chlorella sp. menunjukkan fluktuasi dengan puncak yang berbeda pada Mei-Juli 2005, namun mempunyai pola yang sama pada perioda Agustus-Oktober 2005.Kata kunci : Copepoda, Konsumsi Harian, Chlorella sp.The aim of the research is to know the daily consumption of the copepod on Chlorella sp. cultivated in the different volume of media. The research was conducted from May to October 2005 at Laboratory of BiologicalOceanography, Diponegoro University. Copepods were collected monthly from Demak waters. The copepod cultivations were done using 3 bekers of 2 L and 3 aquariums of 20 L. A stocking density of 100 ind./L ofcopepod for 2 l media and 1000 ind. / l of copepod for 20 l media were used as tests biota per period of cultivation. Chlorella sp. from Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara were givento copepod with density of 15.200 cell/mL. The daily copepod consumption from 06.00–18.00 was observed at 3 hours intervals. The observations were replied monthly for six month. The temperature, salinity, DO and pH were measured daily. The average daily consumption of copepod on Chlorella sp. cultivated in 2 l anad 20 L media were 19,05–140,47 cell/mL and 10,69–102.,6 cell/ml, respectively. The daily consumption of copepod reveal a fluctuated model with differents peak of the consumption during the May – July 2005, while the one of the August – October 2005 show the same model.Key words : Copepods, Daily Consumption, Chlorella sp
Application of Aquaculture Natural Food Produce by Protoplast Fusion Process of Dunaliella salina and Phaffia rhodozyma Hersugondo, Hersugondo; Kusumaningrum, Hermin Pancasakti; Zainuri, Muhammad
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 15, No 4 (2010): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Recently, fishponder income tend to be static so that required an effort to accurately and efficiently one of them through aquacultures  food diversification. So far, efforts to observe the pattern of introduction of a new feed, applications and their impact on aquaculture and fish farmers income has never been measured. Diversification of feed use protoplast fusion process from D. salina and P. rhodozyma which rich in natural carotenoids is needed in aquaculture because they will increase survival and body weight of animal farming. Different types of carotenoids can be combined through a process of protoplasm fusion making it cheaper, faster and efficient for diversification and development of carotenoid-rich diet. The specific objectives of this research is the development of natural food aquaculture with high carotenoid content using protoplasm fusion  from the microalgae D. salina and yeast P. rhodozyma. The early stage in this research will cultivate recombinant followed by quantitative analysis of carotenoid production compared with controls. The second stage is the food diversification with different concentrations of recombinant and comparisons with commercial food followed by cost-benefit analysis. The research results have been obtained which  most carotenoid-rich natural food resulted from recombinant fusion of D. salina and P. rhodozyma. Its application in vitro have increased body weight of shrimp comparing to artificial feed. In addition, recombinant food showing stable growth in both freshwater and salt water, can breed naturally and are safe for animal aquaculture consumption and also the environment. Key words : carotenoid, protoplast fusion, D. salina,  P. rhodozyma, recombinant Selama ini pendapatan petani tambak cenderung statis sehingga diperlukan suatu upaya secara tepat dan efisien salah satunya melalui diversifikasi pakan. Sejauh ini usaha untuk mengamati pola introduksi suatu pakan baru, aplikasi dan dampaknya terhadap budidaya dan pendapatan petani tambak belum pernah terukur. Diversifikasi pakan mengggunakan pakan kaya karotenoid alami sangat dibutuhkan dalam budidaya perikanan karena terbukti dapat meningkatkan keloloshidupan dan menambah bobot hewan budidaya. Kedua jenis karotenoid β-karoten dan astaxantin dapat digabungkan melalui  proses fusi protoplasma sehingga lebih murah, cepat dan efisien untuk diversifikasi dan pengembangan pakan kaya karotenoid.  Tujuan khusus penelitian ini adalah pengembangan usaha budidaya  untuk  meningkatkan  pendapatan  petani  tambak  melalui  diversifikasi  pakan  akuakultur  dengan kandungan karotenoid tinggi hasil fusi protoplasma alga Dunaliella salina dan khamir Phaffia rhodozyma. Tahap awal dalam penelitian ini akan melakukan kultivasi pakan rekombinan Dunaliella salina dan Phaffia rhodozyma diikuti analisis produksi karotenoid secara kuantitatif dibandingkan dengan kontrol. Tahap kedua adalah diversifikasi pakan dengan perbedaan konsentrasi fusan dan perbandingan dengan jenis pakan lain diikuti dengan analisis untung rugi. Kata kunci : karotenoid, fusi protoplas, D. salina,  P. rhodozyma, rekombinan
Molecular Determination of a Gren Algae Isolate to Detecting 1-Deoxy-D-Xylulose-5-phosphate Synthase (DXS) Gene in Improvement of Carotenoid Production Kusumaningrum, Hermin Pancasakti; Soedarsono, Joedono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 11, No 2 (2006): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Sintesis karotenoid alami belum pernah melebihi produk sintetik pada skala komersial. Kurangnya pemahaman mengenai aspek mikrobiologis dan ekofisiologis isolat penghasil karotenoid menyebabkan terjadinya kesalahan penamaan spesies. Satu isolat lokal alga hijau dari BBAP Jepara yang digunakan sebagai pakan alami sumber karotenoid hewan-hewan perikanan, pada mulanya dianggap sebagai Dunaliella. Namun pengembangan produksi karotenoid menggunakan teknologi rekayasa genetik dan rekayasa metabolit terhadap isolat algahijau lebih lanjut memperlihatkan ketidaksesuaian hasil dengan penamaan yang ada. Akumulasi karotenoid jalur non-MVA pada alga hijau ditentukan oleh enzim D-1-Deoksixilulosa 5-fosfat Sintase, yang disandi oleh gen D-1-deoksixilulosa 5-fosfat sintase (DXS). Determinasi spesies secara molekuler menjadi penting dilakukan untuk menentukan spesies isolat dan jalur biosintesis karotenoid yang digunakan. Hasil determinasi digunakan untuk analisis keserupaan putative partial fragment gen DXS Isolat alga hijau yang telah berhasil diperolehpada penelitian sebelumnya. Tujuan utama penelitian ini adalah menentukan spesies satu isolat lokal alga hijau secara molekuler menggunakan 23S rRNA untuk mendeteksi keberadaan gen DXS penyandi biosintesis karotenoid. Hasil penelitian memperlihatkan bahwa Isolat alga hijau menunjukkan keserupaan yang tinggi dengan anggota-anggota Sianobakteria. Keserupaan tertinggi dimiliki dengan  Cyanobacterium sp. MBIC 1021 sebesar 99 %, diikuti Synechocystis PCC6308 sebesar 95 %. Satu-satunya anggota Cyanobacteria yangmemiliki gen DXS adalah Synechocystis. Hasil analisis keserupaan parsial gen DXS isolat alga hijau terhadap tujuh parsial gen DXS pada daerah lestari yang telah ditemukan, memperlihatkan bahwa putative partialfragment gen DXS Isolat lokal alga hijau juga memiliki keserupaan tertinggi dengan gen DXS Sianobakteria Synechocystis.Kata kunci: isolasi alga hijau, Dunaliella, gen DXS, 23S rRNA, Cyanobacteria, SynechocystisCarotenoids production levels are not yet competitive with carotenoid levels presently produced by fermentation, synthesis and isolation. It needs application of metabolic engineering and genetic engineeringtechniques in improving their production. An attempt to optimize carotenoid production from local isolate of green algae from BBAP Jepara has faced several problems, primarily related to the microbiological and ecophysiological characteristic which affecting growth that have not sufficiently been understood. A misnamed of species also have arisen due to wrong characterization. One local isolate of an algal species from BBAPJepara was found potentially useful as source of carotenoids in food additives or as food supplement in fish farming. It was suspected as representing a strain of Dunaliella. Previous studies to improve carotenoidproduction using molecular approach on have shown unagreement. Therefore, the present study aimed to determinate the species of green algae isolate from Jepara waters based on molecular techniques using 23S rRNA approach for detecting DXS gene. Molecular analysis by 23S rRNA alignment showed the close relationship among isolate of green algae and most all of member of Cyanobacteria. Closest similarities wasshowed by Cyanobacterium sp. MBIC 1021 with 99 % similarity and Synechocystis PCC6308 with 95 % similarity. Synechocystis was the only member of Cyanobacteria which have DXS gene. Multiples aligmentsequences of partial DXS gene on the conserve region among seven species confirmed this result. The DXS gene analysis also showed closest relationship between partial DXS gene of Cyanobacteria Synechocystis anda green algae isolate. The result of this analysis proven as valuable parameter for the interpretation of the relation among DXS gene of a green algae isolate and Cyanobacteria and increase the possibility in getting the complete DXS gene from local isolate of green algae by designing primers from DXS gene of Synechocystis as a member of Cyanobcteria.Key words: a green algae isolate, Dunaliella, DXS gene, 23S rRNA, Cyanobacteria, Synechocystis
The Effect of Various Salinity Level on the Growth and Characterization of Dunaliella sp Isolated from Jepara Waters Kusumaningrum, Hermin Pancasakti; Kusdiyantini, Endang; Yuwono, Triwibowo; Sudarsono, Joedoro
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 3 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Dunaliella adalah salah satu biota dengan kandungan β-carotene cukup tinggi. Upaya optimalisasi produksi bcarotene pada Dunaliella berhadapan dengan beberapa masalah kultivasi, untuk mendapatkan species yang paling potensial. Hal ini terkait dengan keterbatasan pengetahuan karakteritik ecophysiologi. Alga hijau Dunaliella diketahui dapat tumbuh pada media dengan kandungan garam yang cukup tinggi, namun karena pemahaman characteristik yang keliru dapat menyebabkan identifikasi yang salah pada satu species dalam genus Dunaliella. Kultur laboratoris pada media microcosms berdasarkan salinitas telah dilakukan untuk mengetahui tingkat pertumbuhan dan karakterisasi Dunaliella sp. dari perairan Jepara. Hasil pengamatan menunjukkan bahwa Dunaliella sp. dapat beradaptasi pada salinitas 0 sampai dengan 30 ‰. Berdasarkan kepada perubahan warna pigmen Dunaliella sp. yang tidak menjadi merah pada media pemeliharaan sampai dengan 25 ‰, maka jenis yang dijumpai di Jepara mempunyai karakter dan secara taksonomis berafiliasi dengan Dunaliella viridis.Kata kunci: Dunaliella sp, Salinity, Growth, CharacterizationDunaliella is one the most enriched β-carotene eucaryotic organism known. The attempt to optimize bcarotene production from Dunaliella has faced with several problems related to its growth management,which was suspectedly unable to meet the needs of the cultured species. This is primarily because the ecophysiological characteristic affecting growth of Dunaliella have not been sufficiently understood. It wasknown that the halophilic species of the green alga Dunaliella was grown in concentrated salt solutions, but based on this characterization, some misnamed of species in genus Dunaliella also have arisen due to wrongcharacterization understanding. Laboratory cultures and mixed-species microcosms were used to asses the growth and characterization of Dunaliella sp. from Jepara Coastal Region with special emphasis on the several factors that affecting growth of organisms including salinity. The result showed that Dunaliella sp. could adapted to a variety of salt concentration from as low as 0.0 % to salt saturation of about 30 ‰. Based on its pigment colour that Dunaliella sp. doesn’t turn red in the growth on salinities up to 25 ‰, it can be characterized and affiliated taxonomically as Dunaliella viridis.Key words: Dunaliella sp, Salinity, Growth, Characterization
SPECIES DETERMINATION OF GREEN ALGAE ISOLATED FROM JEPARA COASTAL REGION BASED ON MICROBIOLOGICAL, ECOPHYSIOLOGICAL AND MOLECULAR CHARACTERIZATION FOR IMPROVEMENT OF CAROTENOID PRODUCTION Kusumaningrum, Hermin Pancasakti; Kusdiyantini, Endang; Yuwono, Triwibowo; Soedarsono, Joedoro
JOURNAL OF COASTAL DEVELOPMENT Vol 10, No 1 (2006): Volume 10, Number 1, Year 2006
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

A local isolate of green algae called C1 from Jepara waters is usually used as a source for carotenoid supplement for animal fisheries in the local area. This indigenous algae has been successfully purificated.  Although the local isolate was known as eucaryotic green algae Dunaliella, our previous molecular study by 18S rDNA analysis to determine the species of this organisms showed negative result. In order to improve carotenoid production especially detection of biosynthetic pathway from the organisms investigated in this study, the main purpose of this study was species determination of local isolate of green algae based on microbiologycal, ecophysiologycal and molecular characterization. The results of this research  indicated that local isolate of green algae posseses Cyanobacteria characteristic, especially Synechocystis. Analyses with 16S rDNA sequence from genome of green algae isolates were also in accordance with these results, showing close similarities with Synechocystis 16S rDNA sequence.  However, it should be noted that, instead of having Synechocystis dominant feature, it was also found that local isolate of green algae exhibit different characteristic in having chlorophyll a, chlorophyll b and lack of phycobillins. This character was typical for abberant Cyanophyta, Prochlorophyta.
IDENTIFIKASI MOLEKULER TANAMAN PISANG RAJALAWE BERDASARKAN GEN INTERNAL TRANCRIBED SPACER (ITS) Fardilla, Firly Putri; Kusumaningrum, Hermin Pancasakti; wijanarka, w
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Banana is one type of horticultural commodities in a group of fruits that have a socio-economic value is high enough for the people of Indonesia. Bananas have different varieties, one banana type Rajalawe found in Central Java. Rajalawe molecular identification has not been done before. This study aims to determine the result of the identification of the molecular basis of  Rajalawe based on genes Internal transcribed spacer (ITS), in search of identity and kinship Rajalawe. The study was conducted by isolating DNA using a method Rajalawe Doyle & Doyle, followed by ITS gene amplification and sequencing analysis. The results of gene amplification ITS produce PCR product of 643 bp. The base sequence of the sequencing results are used for the construction of phylogenetic trees. Sequence similarity analysis Rajalawe show 95% homology with Musa balbisiana and alkaline difference of 1%. Phylogenetic tree analysis showed Rajalawe have a close relationship with Musa balbisiana. However, bananas Rajalawe has several different characters with Musa balbisiana with different base sequences by 5% whereas the base sequence homology between the banana Musa balbisiana and Rajalawe with 95%.Keywords: Molecular Identification, Pisang Rajalawe, Universal Primer ITS, Musa balbisiana.
KERAGAMAN GENETIK JAHE (Zingiber officinale Roscoe) MENGGUNAKAN TEKNIK PENANDA MOLEKULER Random Amplified Polymorphic DNA (RAPD) Putri, Choirul Rizka; Kusumaningrum, Hermin Pancasakti; Kusdiyantini, Endang
Jurnal Akademika Biologi Vol. 5 No. 2 April 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Ginger plant in Indonesia is known there are three kinds of varieties, namely red ginger, emprit ginger and big ginger. Ginger (Zingiber officinale Roscoe) is one of the medicinal plants that could potentially be produced in increasing quantities and wide distribution in Indonesia that may affect patterns of genetic diversity. This study aims to determine patterns of genetic diversity of three varieties of ginger (red ginger, big ginger and emprit ginger) obtained from BALITRO, Bogor and BPTP, Ungaran using two primers (OPA1 and OPA2). Techniques of molecular markers RAPD (Random Amplified Polymorphic DNA) is one of the techniques used for the analysis of genetic diversity. The study begins by isolating DNA from 30 samples of ginger leaves, then amplification with primers OPA1 and OPA2 which then results of electrophoresis converted into the form of binary data and phylogenetic tree construction with 2:02 NYTSYS program. The results obtained are worth a total of 20 polymorphic band (86.9%) and 3 pita monomorphic (13%) with primary OPA1 and OPA2. Heterozygosity values in a row is worth 0.9026 and 0.889. Distance genetic similarities in this sample has the highest value is 1 and the lowest is 0.3. The relationship between the three varieties of big ginger are distant relationship with red ginger and emprit ginger. Red ginger and emprit ginger still have a close genetic relationship. Key Word               : Genetic Diversity, Zingiber officinale, RAPD
Pertumbuhan Mikroalga Botryococcus braunii Sebagai Penghasil Lipid Pada Medium Campuran Antara Air Kelapa Dan Air Laut Saputro, Bintoro Rudi; Kusdiyantini, Endang; Kusumaningrum, Hermin Pancasakti
Jurnal Akademika Biologi Vol. 4 No. 4 Oktober 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Energy needs in fuel sector tend to increase for supporting human life. Green microalgae (Chlorophyceae) can be used as an alternative solutions  for bioenergy.  Botryococcus braunii is lipid producer microalgae which can be processed further into biodiesel. Microalgae growth medium such as Walne can be subtituted with coconut water that reducing economical cost. This research aims to determine the growth of B. braunii using coconut water and its effect on lipid production. The experiment conducted into five treatments of coconut water consist of  P0 (0%), P1 (10%), P2 (7.5%), P3 (5%), and P4 (2.5%) for five days incubation and three repetitive step. Microalgae growth was calculated according to cell count using haemocytometer. The results showed that 2.5% coconut water subtitution exhibited the best growth rate and lipid production, ie the amount of 547 cells / ml and produced lipid level according to these treatment increased 179% comparing with control. Keywords: Growth, Botryococcus braunii, Lipid, Coconut water, Seawater.
Karakterisasi Dan Identifikasi Molekuler Fusan Hasil Fusi Protoplas Interspesies Chlorella pyrenoidosa dan Chlorella vulgaris Menggunakan 18SrDNA Rismiarti, Asih; Kusumaningrum, Hermin Pancasakti; Zainuri, Muhammad
Bioma Vol. 18, No.1, Tahun 2016
Publisher : Bioma

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Abstract

Chlorella pyrenoidosa is a unicellular green algae that grows in fresh water with carotenoids consisting of             β-carotene, α-carotene, anthaxanthin, neoxanthin, zeaxanthin and lutein. C. vulgaris usually it lives in sea water with carotenoids, chlorophyll, tocopherol, ubiquinone and proteins. The quality of them is improved by protoplast fusion and identified further using moleculer analysis. This study aims to find out the characterization and identification of molecular fusan that is obtained from interspecies C. pyrenoidosa and C. vulgaris protoplast fusion process using 18SrDNA. Both C. pyrenoidosa and C. vulgaris are combined by protoplast fusion and then they were performed the isolation of DNA with CTAB modification method, followed by PCR gradient using primers 18S Chlorella and performed DNA sequencing. The result show that there are different characters between masterplan and fusan based on growth of fresh water and sea water medium. The success frequency of fusan as a result from protoplast fusion in the fresh water media is 21% and 6% for sea water medium. The results of the alignment between fresh water fusan and C. vulgaris masterplan from GeneBank shows that the base sequence homology is 93% C. pyrenoidosa masterplan from GeneBank is 90%. The result of molecular identification towards the sequence of fresh water fusan bases shows that there is a kinship relationship with the masterplan of C. pyrenoidosa 18S Chlorella and Chlorosphaera klebsii microalgae compared with some other species from Chlorophyta group with similarity value as many as 91%. It shows that the high variety genetic is based on variations of the base sequence and has a kinship with other species in the Chlorophyta group.                                      Keywords : Chlorella pyrenoidosa, Chlorella vulgaris, Protoplast Fusion, DNA Sequensing, 18SrDNA.
Biologi Kusumaningrum, Hermin Pancasakti
SAINS DAN MATEMATIKA Volume 20 Nomor 4 Tahun 2012
Publisher : SAINS DAN MATEMATIKA

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