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Keagresifan Beberapa Isolat Fusarium oxysporum f. sp. zingiberi Asal Temanggung dan Boyolali Setelah Penyimpanan dalam Tanah Steril Wahyu, Henky Setyawan Norandika; Soesanto, Loekas; Kustantinah, Kustantinah
Jurnal Fitopatologi Indonesia Vol 8, No 6 (2012)
Publisher : Jurnal Fitopatologi Indonesia

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Abstract

Preservation of fungi isolates is needed for further research but usually the preservation treatment is only concerned on life span and growth of the fungi. This research aimed at knowing life span and aggressivity of Fusarium oxysporum f. sp. zingiberi isolates from ginger cv. Gajah after preserving for three years in sterile soil. Completely Randomized Design and Randomized Block Design were used for in vitro and in planta test, respectively, with 10 isolates of F. oxysporum f. sp. zingiberi from Temanggung, 8 isolates of F. oxysporum f. sp. zingiberi from Boyolali, and 1 control, each repeated three times. Variables observed were incubation period, infected area, disease intensity, rhizome wet weight, number of leaves, and crop height. Result of the research showed that all isolates of F. oxysporum f. sp. zingiberi could still grew well. Based on origin of the isolates, the highest aggressivity on ginger rhizome was TKO3 from Temanggung and BAO6 from Boyolali with infected area of 67.67 mm2 and 56.67 mm2, respectively. On ginger crop, the highest aggressivity was BAO1 from Boyolali and TPO2 from Temanggung with disease intensity of 68.09% and 38.13%, respectively. All isolates of F. oxysporum f. sp. zingiberi did not cause significantly different effect on incubation period, disease intensity, crop height, leaves total, and rhizome wet weight but they caused significantly different effect on infected area.   Key word: aggressivity, Fusarium oxysporum, ginger, preservation, steril soil
Genetic Analysis Using Partial Sequencing of Melanocortin 4 Receptor (MC4R) Gene in Bligon Goat Latifah, Latifah; Priyadi, D. A.; Maharani, D.; Kustantinah, Kustantinah; Hartatik, T.
Media Peternakan Vol 40, No 2 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/medpet.2017.40.2.71

Abstract

Melanocortin 4 Receptor gene is involved in sympathetic nerve activity, adrenal and thyroid functions, and media for leptin in regulating energy balance and homeostasis. The aim of this research was to perform genetic analysis of MC4R gene sequences from Bligon goats. Fourty blood samples of Bligon does were used for DNA extraction. The primers were designed after alignment of 12 DNA sequences of MC4R gene from goat, sheep, and cattle. The primers were constructed on the Capra hircus MC4R gene sequence from GenBank (accession No. NM_001285591). Two DNA polymorphisms of MC4R were revealed in exon region (g.998 A/G and g.1079 C/T). The SNP g.998 A/G was a non-synonymous polymorphism i.e., changing of amino acid from methionine (Met) to isoleucine (Ile). The SNP g.1079 C/T was a synonymous polymorphism. Restriction enzyme mapping on Bligon goat MC4R gene revealed three restriction enzymes (RsaI (GT?AC), Acc651 (G?GTAC_C), and KpnI (G_GTAC?C), which can recognize the SNP at g.1079 C/T. The restriction enzymes may be used for genotyping of the gene target using PCR-RFLP method in the future research.
Virulensi Fusarium oxysporum f.sp. zingiberi Isolat Boyolali dan Temanggung setelah Disimpan Enam Tahun dalam Tanah Steril Riyadi, Andry Slamet; Soesanto, Loekas; Kustantinah, Kustantinah
Jurnal Perlindungan Tanaman Indonesia Vol 14, No 2 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11891

Abstract

This research aimed to study growth ability and virulence of several Fusarium oxysporum f.sp. zingiberi isolates originated from Boyolali and Temanggung after being preserved for six years in sterile soils media. Completely Randomized Design was used with 12 treatments and 3 replications. F. oxysporum f.sp. zingiberi originated from Temanggung consisted of 7 isolates, i.e., TKO1, TKO3, TKO4, TKO6, TKO7, TPO1, TPO5; and from Boyolali consisted of 4 isolates, i.e., BAO2, BAO7, BAC, and BAP. Variables observed were growth on PDA, colony color, colony diameter, macroconidia and microconidia, mycelial dry weight, incubation period, attack area, and difference of fresh weight of rhizome. The result showed that all isolates of F. oxysporum f.sp. zingiberi both from Temanggung and Boyolali were able to grow well on PDA and fully covered the Petridish at 4.75–7.5 days. The most virulent isolate was TKO6 from Temanggung showing the fastest incubation period of 5 days after inoculation and the highest attack area of 420 mm2 or increase for 107.6%. Penelitian ini bertujuan untuk mengetahui viabilitas dan virulensi beberapa isolat Fusarium oxysporum f.sp. zingiberi setelah disimpan selama enam tahun pada tanah steril. Rancangan yang digunakan adalah Rancangan Acak Lengkap dengan tiga ulangan dan 12 perlakuan. Isolat F. oxysporum f.sp. zingiberi yang digunakan asal Temanggung terdiri atas tujuh isolat, yaitu TKO1, TKO3, TKO4, TKO6, TKO7, TPO1, dan TPO5; asal Boyolali terdiri atas empat isolat, yaitu BAO2, BAO7, BAC, dan BAP. Parameter yang diamati adalah pertumbuhan isolat pada medium PDA, warna koloni, diameter koloni, makrokonidium dan mikrokonidium, berat kering miselium, masa inkubasi, luas serangan, dan selisih berat basah rimpang. Hasil penelitian menunjukkan bahwa semua isolat F. oxysporum f.sp. zingiberi asal Temanggung dan Boyolali, yang telah disimpan dalam medium tanah steril selama enam tahun, masih tumbuh de-ngan baik pada medium PDA dan memenuhi cawan Petri pada umur 4,75–7,5 hari. Isolat yang paling virulen adalah TKO6 asal Temanggung dengan masa inkubasi tercepat yaitu 5 hari setelah inokulasi dan luas serangan pada rimpang sebesar 420 mm2 atau ada peningkatan sampai 107,6%
Lama Kebuntingan, Litter Size, dan Bobot Lahir Kambing Boerawa pada Pemeliharaan Perdesaan di Kecamatan Gisting Kabupaten Tanggamus Adhianto, Kusuma; Ngadiyono, Nono; Kustantinah, Kustantinah; Budisatria, I Gede Suparta
Jurnal Penelitian Pertanian Terapan Vol 12, No 2 (2012)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (46.142 KB) | DOI: 10.25181/jppt.v12i2.208

Abstract

An experiment was conducted on 100 heads of Boerawa doe mated with male Boer. The parameters perceived were pregnancy periode, litter size, and birth weight. The study was undertaken at Boerawa goat development area Kecamatan Gisting Kabupaten Tanggamus. The data were analysed in SPSS versi 10. The results showed that pregnancy periode 159,31 + 4,37 days, litter size 1,62 + 0,65 heads, and birth weight 3.02 + 029 kg. Keywords: boerawa goat, pregnancy periode, litter size, birth weight
Pengaruh Flushing Berbasis Pakan Lokal terhadap Pertumbuhan dan Birahi Kambing Kejobong Betina Dewasa Socheh, M; Ismaya, Ismaya; S. Budisatria, I G; Kustantinah, Kustantinah
Sains Peternakan: Jurnal Penelitian Ilmu Peternakan Vol 9, No 2 (2011): Sains Peternakan
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/sainspet.9.2.53-64

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The purposes of this study were to: 1. Study whether flushing based local feed affect growth of Kejobong doe. 2. Study whether flushing based local feed affect the response of estrous, the initial of estrous, and the length of estrous. Twenty Kejobong does with average initial live weight 26.6 ± 1.7 kg were used in this study. The animals were placed randomly into two feed treatments, namely: non-flushing (p0) and flushing feed (p1) with 10 replicates each. The observed variables were ADG, estrous response, the initial of estrous and the length of estrous. The difference value observed for the treatment of non-flushing and flushing analyzed by t test. The results showed that the growth of Kejobong doe at flushing group was higher (P≤0.05) than non-flushing group. Response of estrous both in the group of nonflushing and flushing were 100%. The initial of estrous of Kejobong doe from flushing group was more quickly than non-flushing (P≤0.05). The length of estrous of flushing group waslonger (P≤0.05) than non-flushing group. It can br concluded that flushing on Kejobong doe can improve growth, accelerate the initial of estrous and prolong the length of estrous.Key words: flushing, non-flushing, growth, estrous, Kejobong doe
Penggunaan Teknik In Sacco Mobile Sebagai Estimasi Kecernaan Nutrien Hijauan Pakan Ternak di dalam Intestinum Hadi, Rendi Fathoni; Kustantinah, Kustantinah; Hartadi, Hari
Sains Peternakan: Jurnal Penelitian Ilmu Peternakan Vol 14, No 2 (2016): Sains Peternakan
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/sainspet.14.2.12-21

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This study aims to explore the potential of forage optimally, the degradation rate of forage nutrients are usually given by the farmers at the time in the intestine of a variety of forage fodder. This study used four kinds of forage feed material: Gliricidia leaves (G), Manilkara leaves (S), Ficus leaves (B) and Cocoa leaves (C). This study was observed in intestinal digestibility. The data taken is nutrient digestibility of rumen and intestinal residues include DM, OM and CP. Samples were incubated in the intestine of cattle that canulation intestine with different time intervals, time/point of incubation: 2, 4, 8, 12, 16, 24, 36 and 48 hours. Samples were incubated in livestock that fistulation intestinal samples of rumen residue. The results showed that the digestibility of nutrients DM, OM and CP respectively forage percentage amount of intestinal digestibility value of feedstuffs in the rumen incubation time of 2-48 hours progressively decreased the percentage of nutrient digestibility. The conclusion of the study when it is time to stay forage in the rumen longer then the more nutrients are degraded by rumen microbes that escapes rumen degradation will be more and more and can be digested in the intestine will be less. Keywords: in sacco mobile, forage, intestine
Tenderness and the Calpain System of Three Different Types of Muscles of Kejobong Does under Two Different Energy Levels Socheh, M; Ismaya, Ismaya; Budisatria, I Gede Suparta; Kustantinah, Kustantinah
ANIMAL PRODUCTION Vol 14, No 1 (2012): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Abstract. The purpose of research was to investigate whether there was influence of the addition of concentrates in basal feed on meat tenderness and calpastatin and calpain activities of three muscles of Kejobong does. A total of 20 heads of Kejobong does (2.0-2.5 years) with initial live weight range between 23.8-24.2 kg was studied and randomly set in two kinds of feed treatment, ie basal feed (p0) and basal feed plus concentrates (p1). Two kinds of feed were given to Kejobong doe for 12 weeks.   The observed variables were (1) tenderness of meat, (2) calpain activity (m-calpain activity and the activity of m-calpain), and (3) calpastatin activity. Data were processed using the General Linear Model Univariate procedure and Pearson Bivariate Correlation of the SPSS program.  Results showed that feeding (basal feed and basal feed plus concentrates) significantly affected meat tenderness of Kejobong doe. The types of muscles had highly significant effect on tenderness. Feeds affected m-calpain activity, m-calpain activity, and calpastatin activity in the muscles. Types of muscles exhibited variation in the activity of m-calpain, the activity of m-calpain, and calpastatin activity.  In conclusion, the addition of concentrates in the basal feed increased meat tenderness, and affected the activity of m-calpain, m-calpain, and calpastatin.Keywords:  activity of m-calpain and m-calpain, calpastatin activity, Kejobong doeAnimal Production 14(1):47-55, January 2012
Doe Productivity of Boerawa Goat on Rural Condition Adhianto, K; Ngadiyono, N; Budisatria, I Gede Suparta; Kustantinah, Kustantinah
ANIMAL PRODUCTION Vol 15, No 1 (2013): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Abstract. This research was carried out at Gisting sub-District, Tanggamus Regency, in order to find out doe productivity of Boerawa goat on rural on condition. This research involved 120 doe Boerawa goat housed in elevated barns. Measurements were performed to evaluate the litter size, birth weight, weight at weaning, and pre-weaning mortality. The results of the present research showed that birth weight of the kids was 3.10 kg and 2.94 kg for male and female, respectively. The average birth weight was based on the type of birth 3.20 kg and 3.04 kg for the single versus twin born kids. It was documented that the average weight at weaning of the kids were 17.12 kg and 16.23 kg for male and female, respectively. Whereas based on birth type, the average weight was of the single born kids and the twin at weaning were 17.30 versus 16.63 kg. In addition, doe reproduction index was 2.36 weaned kids/year, and the average doe productivity was 41.52 kg. Keywords: Boerawa goat, doe productivity, rural condition. Abstrak.  Penelitian ini dilakukan di Kecamatan Gisting Kabupaten Tanggamus, dalam rangka untuk mengetahui produktivitas induk kambing Boerawa pada kondisi pedesaan. Penelitian ini melibatkan 120 induk kambing Boerawa yang dipelihara di kandang panggung. Pengukuran dilakukan untuk mengevaluasi ukuran jumlah cempe sekelahiran, bobot lahir, bobot sapih, dan kematian pra-sapih. Hasil dari penelitian ini menunjukkan bahwa bobot lahir cempe adalah 3,10 kg dan 2,94 kg (masing-masing untuk jantan dan betina). Bobot lahir rata-rata didasarkan pada tipe kelahiran tunggal dan kembar adalah masing-masing 3.20 kg dan 3,04 kg. Diperoleh hasil bahwa bobot sapih adalah 17,12 kg dan 16,23 kg (masing-masing untuk jantan dan betina). Sedangkan berdasarkan tipe kelahiran, bobot sapih rata-rata dari tipe kelahiran tunggal dan kembar adalah 17.30 dan 16.63 kg. Selain itu, indeks reproduksi induk sebesar 2,36 cempe sapihan/tahun, dan produktivitas induk rata-rata adalah 41,52 kg. Kata kunci: Kambing Boerawa, produktivitas induk , kondisi pedesaan.K Adhianto et al./Animal Production 15(1):31-39, January 2013
Physicochemical Characteristics Identification and Secondary Metabolite Analysis of Solid Herbal Waste as Source of Feed Rich Fiber and Supplement for Ruminants Kisworo, Arif Nindyo; Agus, Ali; Kustantinah, Kustantinah; Suwignyo, Bambang
ANIMAL PRODUCTION Vol 18, No 2 (2016): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.anprod.2016.18.2.535

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A study was conducted to determine the potency, physicochemical characteristics, and analyzed the secondary metabolites content of solid herbal waste (SWH) as a substitute source of feed rich fibre and feed supplements in ruminants. The first study includes an analysis of production potential, physical analysis, and chemical composition analysis of SWH. The second study was an analysis of secondary metabolites content of SWH. The results showed that SWH volume reached 6-8 m3/day or 4020-5360 kg/day, the chemical composition of herbal solid waste was similar to king grass with high lignin content (17.53%). SWH containing total phenols, flavonoids, tannins, saponins , alkaloids and essential oils which generally have antimicrobial activity. According to the potential availability, chemical composition and secondary metabolites content of SWH, it can be used as an alternative for substitute of feed rich fiber or feed supplements with attention to the content of secondary metabolites that can affect the process of fermentation and digestibility in the rumen. Further in vitro and in vivo research ore needed to determine the effect of SWH on rumen fermentation parameters and its application in the ration in ruminant livestock.
Nutritional Status Evaluation (Estimated Using Lignin Internal Indicator) of Grazed Yearling Kacang Bucks Adiwiniarti, Retno; Budisatria, I Gede Suparta; Kustantinah, Kustantinah; Rusman, Rusman; Purnomoadi, Agung
ANIMAL PRODUCTION Vol 17, No 3 (2015): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.anprod.2015.17.3.522

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In some areas in Indonesia, farmers usually graze their goats during the day and house them at night.  The goats eat grass without additional concentrate.  However, how much nutrient intakes of grazing goats in Indonesia are seldom calculated. Therefore, this research was set up to investigate the productivity and to estimate the dry matter, protein, and TDN intake of grazed Kacang buck using lignin internal indicator.  About 12 heads of Kacang buck, aged 1-1.5 year (the incisors have erupted 1), having the initial body weight of 18.42+1.78 kg (CV= 9.67%) and reared by farmers in Grobogan, Central Java, Indonesia were used in this study.  Intake and nutrient digestibility of the buck were calculated using lignin as internal marker.  After being reared 86 days, the average daily gains (ADG) of the buck were 3.20+21.14 gram ( -26.16 gram to 53.60 gram). Low ADG of the buck was caused by low DMI, protein and TDN intake.  The Kacang bucks were usually grazed in the soccer fields, back yards, and roadsides, therefore their intake were lower than their maintenance needed.  The averages of DMI, protein and TDN intake were about 1.17+0.17 % their body weight, 30.16+4.18 g and 91.45+11.64 g, respectively. It can be concluded that the productivity of grazing Kacang bucks is relatively very low.