Articles

KERAGAMAN GENETIK JAHE (Zingiber officinale Roscoe) MENGGUNAKAN TEKNIK PENANDA MOLEKULER Random Amplified Polymorphic DNA (RAPD)

Jurnal Akademika Biologi Vol. 5 No. 2 April 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Ginger plant in Indonesia is known there are three kinds of varieties, namely red ginger, emprit ginger and big ginger. Ginger (Zingiber officinale Roscoe) is one of the medicinal plants that could potentially be produced in increasing quantities and wide distribution in Indonesia that may affect patterns of genetic diversity. This study aims to determine patterns of genetic diversity of three varieties of ginger (red ginger, big ginger and emprit ginger) obtained from BALITRO, Bogor and BPTP, Ungaran using two primers (OPA1 and OPA2). Techniques of molecular markers RAPD (Random Amplified Polymorphic DNA) is one of the techniques used for the analysis of genetic diversity. The study begins by isolating DNA from 30 samples of ginger leaves, then amplification with primers OPA1 and OPA2 which then results of electrophoresis converted into the form of binary data and phylogenetic tree construction with 2:02 NYTSYS program. The results obtained are worth a total of 20 polymorphic band (86.9%) and 3 pita monomorphic (13%) with primary OPA1 and OPA2. Heterozygosity values in a row is worth 0.9026 and 0.889. Distance genetic similarities in this sample has the highest value is 1 and the lowest is 0.3. The relationship between the three varieties of big ginger are distant relationship with red ginger and emprit ginger. Red ginger and emprit ginger still have a close genetic relationship. Key Word               : Genetic Diversity, Zingiber officinale, RAPD

Konsumsi Harian Copepoda terhadap Pakan Chlorella sp. pada Volume Media Kultivasi yang Berbeda

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 13, No 3 (2008): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Penelitian ini bertujuan untuk mengetahui konsumsi harian copepoda terhadap Chlorella sp. pada volume media kultivasi yang berbeda. Penelitian ini dilaksanakan dari Mei hingga Oktober 2005 di Laboratorium Biologi Oseanografi UNDIP. Copepoda diambil per bulan dari bulan Mei hingga Oktober 2005 di Perairan Demak. Chlorella sp. digunakan sebagai pakan copepoda, berasal dari Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara, dengan densitas 15.200 sel/mL. Kultivasi copepoda menggunakan 3 beker 2 L dengan densitas awal 100 ind./L dan 3 akuarium 20 L dengan densitas awal 1000 ind./L. Pengamatan konsumsi harian copepoda terhadap Chlorella sp. dilakukan per 3 jam dari pukul 06.00 sampai 18.00. Perioda kultivasi diulang per bulan selama 6 bulan. Parameter suhu, salinitas, DO dan pH diamati setiap hari. Konsumsi rata-rata copepoda terhadap Chlorella sp. menunjukkan 19,05- 140,47 sel/mL/jam pada media 2 L dan 10,69—102,06 sel/mL/jam pada media 20 L. Model konsumsi copepoda terhadap Chlorella sp. menunjukkan fluktuasi dengan puncak yang berbeda pada Mei-Juli 2005, namun mempunyai pola yang sama pada perioda Agustus-Oktober 2005.Kata kunci : Copepoda, Konsumsi Harian, Chlorella sp.The aim of the research is to know the daily consumption of the copepod on Chlorella sp. cultivated in the different volume of media. The research was conducted from May to October 2005 at Laboratory of BiologicalOceanography, Diponegoro University. Copepods were collected monthly from Demak waters. The copepod cultivations were done using 3 bekers of 2 L and 3 aquariums of 20 L. A stocking density of 100 ind./L ofcopepod for 2 l media and 1000 ind. / l of copepod for 20 l media were used as tests biota per period of cultivation. Chlorella sp. from Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara were givento copepod with density of 15.200 cell/mL. The daily copepod consumption from 06.00–18.00 was observed at 3 hours intervals. The observations were replied monthly for six month. The temperature, salinity, DO and pH were measured daily. The average daily consumption of copepod on Chlorella sp. cultivated in 2 l anad 20 L media were 19,05–140,47 cell/mL and 10,69–102.,6 cell/ml, respectively. The daily consumption of copepod reveal a fluctuated model with differents peak of the consumption during the May – July 2005, while the one of the August – October 2005 show the same model.Key words : Copepods, Daily Consumption, Chlorella sp

Struktur Komunitas Copepoda di Perairan Jepara

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 12, No 4 (2007): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Copepoda merupakan komponen terbesar dari zooplankton di laut dan berperan sebagai produser sekunder serta konsumer. Berdasarkan perannya dalam jaring-jaring makanan, maka komunitas copepoda pada suatu perairan dapat digunakan untuk menilai produktivitas perairan. Penelitian ini bertujuan untuk mengetahui struktur komunitas copepoda di perairan Jepara. Penelitian ini dilaksanakan dari April hingga Desember 2005 di perairan Jepara dan identifikasi dilakukan di Laboratorium Biologi Oseanografi UNDIP. Enam stasiun untuk pengambilan sampel yang dilakukan sebulan sekali. Pengambilan sampel copepoda dilakukan dengan planktonnet 100 mm, dengan menyaring air sebanyak 1 m3 secara vertikal dari dasar perairan. Sampel yang diperoleh diawetkan dengan formalin 4% untuk diidentifikasi di laboratorium. Keanekaragam, keseragaman dan dominansi dianalisa untuk mengetahui struktur komunitas copepoda. Pengukuran parameter kualitas air yang meliputi suhu, salinitas pH, arus dan kecerahan dilakukan secara bersamaan dengan pengambilan sampel. HasiI penelitian di perairan Jepara terdapat3 ordodan 18 genus copepoda, yaitu Ordo Calanoida (13 genus), Cyclopoida (2 genus) dan Harpacticoida (3 genus). Kelimpahan copepoda di perairan Jepara sebesar 5 - 546 ind/l, dengan rata-rata 316 ± 85, keanekaragaman 2,3259-2,3594, keseragaman 0,80 - 0,81 dan terdapat dominansi Acartia sp dan Calanus sp. Kata kuncl: Copepoda, Struktur Populasi, Jepara The dominantion ofcopepod in the zooplankton community, play an important role as secondary producer and primary consumer in the sea food web. Due to this function, the copepod population can be use as the sea water productivity. The aim of the research is to know and copepod community structure at Jepara Waters. The research was conducted from April to December 2005 at the Jepara Wafers and the samples were identified at Laboratory of Biological Oceanography, UNDIP. There were six stations established as the research site area. Monthly sampling was done. Copepod were collected using the 100 mm plankton net, by filtering a total of 1 m3 sea water taken vertically. The samples were preserved by the addition of formaldehyde 4% and identified in the laboratory. The diversity, evenness and dominance index were calculated to describe the copepod community structure. The water quality such as temperature, salinity, pH, current and transparency, were observed in the same time. There were 18 genus of copepods determined at the Jepara waters, belong to 3 ordo i.e Calanoid (13 genus), Cydopoid (2 genus) and Harpacticoid (3 genus). Copepod density at Jepara water was 5-546 ind./l, (average 316 ± 85), diversity 2,3259-2,3594, eveness 0,8047-0,8163 and dominancy 0,1837-0,1953. Acartia sp and Calanus sp. were dominant in Jepara waters identified. Key words : Copepods, Population Structure, Jepara

The Effect of Various Salinity Level on the Growth and Characterization of Dunaliella sp Isolated from Jepara Waters

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 3 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Dunaliella adalah salah satu biota dengan kandungan β-carotene cukup tinggi. Upaya optimalisasi produksi bcarotene pada Dunaliella berhadapan dengan beberapa masalah kultivasi, untuk mendapatkan species yang paling potensial. Hal ini terkait dengan keterbatasan pengetahuan karakteritik ecophysiologi. Alga hijau Dunaliella diketahui dapat tumbuh pada media dengan kandungan garam yang cukup tinggi, namun karena pemahaman characteristik yang keliru dapat menyebabkan identifikasi yang salah pada satu species dalam genus Dunaliella. Kultur laboratoris pada media microcosms berdasarkan salinitas telah dilakukan untuk mengetahui tingkat pertumbuhan dan karakterisasi Dunaliella sp. dari perairan Jepara. Hasil pengamatan menunjukkan bahwa Dunaliella sp. dapat beradaptasi pada salinitas 0 sampai dengan 30 ‰. Berdasarkan kepada perubahan warna pigmen Dunaliella sp. yang tidak menjadi merah pada media pemeliharaan sampai dengan 25 ‰, maka jenis yang dijumpai di Jepara mempunyai karakter dan secara taksonomis berafiliasi dengan Dunaliella viridis.Kata kunci: Dunaliella sp, Salinity, Growth, CharacterizationDunaliella is one the most enriched β-carotene eucaryotic organism known. The attempt to optimize bcarotene production from Dunaliella has faced with several problems related to its growth management,which was suspectedly unable to meet the needs of the cultured species. This is primarily because the ecophysiological characteristic affecting growth of Dunaliella have not been sufficiently understood. It wasknown that the halophilic species of the green alga Dunaliella was grown in concentrated salt solutions, but based on this characterization, some misnamed of species in genus Dunaliella also have arisen due to wrongcharacterization understanding. Laboratory cultures and mixed-species microcosms were used to asses the growth and characterization of Dunaliella sp. from Jepara Coastal Region with special emphasis on the several factors that affecting growth of organisms including salinity. The result showed that Dunaliella sp. could adapted to a variety of salt concentration from as low as 0.0 % to salt saturation of about 30 ‰. Based on its pigment colour that Dunaliella sp. doesn’t turn red in the growth on salinities up to 25 ‰, it can be characterized and affiliated taxonomically as Dunaliella viridis.Key words: Dunaliella sp, Salinity, Growth, Characterization

SPECIES DETERMINATION OF GREEN ALGAE ISOLATED FROM JEPARA COASTAL REGION BASED ON MICROBIOLOGICAL, ECOPHYSIOLOGICAL AND MOLECULAR CHARACTERIZATION FOR IMPROVEMENT OF CAROTENOID PRODUCTION

JOURNAL OF COASTAL DEVELOPMENT Vol 10, No 1 (2006): Volume 10, Number 1, Year 2006
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

A local isolate of green algae called C1 from Jepara waters is usually used as a source for carotenoid supplement for animal fisheries in the local area. This indigenous algae has been successfully purificated.  Although the local isolate was known as eucaryotic green algae Dunaliella, our previous molecular study by 18S rDNA analysis to determine the species of this organisms showed negative result. In order to improve carotenoid production especially detection of biosynthetic pathway from the organisms investigated in this study, the main purpose of this study was species determination of local isolate of green algae based on microbiologycal, ecophysiologycal and molecular characterization. The results of this research  indicated that local isolate of green algae posseses Cyanobacteria characteristic, especially Synechocystis. Analyses with 16S rDNA sequence from genome of green algae isolates were also in accordance with these results, showing close similarities with Synechocystis 16S rDNA sequence.  However, it should be noted that, instead of having Synechocystis dominant feature, it was also found that local isolate of green algae exhibit different characteristic in having chlorophyll a, chlorophyll b and lack of phycobillins. This character was typical for abberant Cyanophyta, Prochlorophyta.

ISOLASI, KARAKTERISASI BAKTERI ASAM LAKTAT, DAN ANALISIS PROKSIMAT DARI PANGAN FERMENTASI RUSIP IKAN TERI (Stolephorus sp.)

Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Rusip is a traditional food from Bangka-Belitung made from fresh anchovy with salt and palm sugar that is brooded for seven days. Rusip fermentation process is caused by microbial activity mainly from lactic acid bacteria. The purpose of this study was to isolate lactic acid bacteria from rusip, to characterize isolates obtained based on common characteristic of lactic acid bacteria, and to carry out proximate analysis of rusip anchovy. Isolation from rusip fermented food obtained eight bacterial isolates, five isolates were circular and three isolates were rod-shaped. Characterization and identification of lactic acid bacterial were performed using tests that led to the common characteristic of lactic acid bacteria, i.e the gram staining, motility test, acid formation test, catalase test, and proteolytic test. The result showed that eight bacterial isolates were gram-positive, non-motile, catalase-negative, positive to produce acid, and had proteolytic activity. Rusip anchovy had 0,64% of total lactic acid and the pH 5,40. Nutrient content of rusip fermentation had 10,3644% moisture content; 50,0702% ash content; 0,9524% crude fat content; 0,3764% crude fiber content; 34,8603% crude protein content; and 14,1171% carbohydrate content. Keywords: rusip, isolation, characterization, lactic acid bacteria, proximate analysis

ISOLASI, KARAKTERISASI BAKTERI ASAM LAKTAT, DAN ANALISIS PROKSIMAT DARI MAKANAN FERMENTASI BEKASAM IKAN MUJAIR (Oreochromis mossambicus Peters)

Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bekasam is one of the traditional fermented food made from freshwater fish with the addition of salt and carbohydrate sources. Mozambique fish is used as a raw material, because it has high protein content and can be easily obtained in the market at affordable prices. Mozambique fish is used to make bekasam with the addition of salt as much as 18% of the weight of the fish and brooded for 48 hours, then added with toasted rice as much as 15% of the weight of the fish and fermented for 7 days. This study aimed to isolate and characterize lactic acid bacteria and analyze the nutritional value of mozambique fish bekasam. The methods used were  isolation, characterization and proximate analysis of the mozambique fish bekasam. Isolation of lactic acid bacteria was carried out on mozambique fish as a control, mozambique fish before and after the addition of toasted rice. Isolation obtained 6 isolates of Gram positive bacteria, 5 isolates of cocci shaped bacteria and 1 rod shaped bacteria. Based on morphological and physiological tests, six isolates were Gram positive, non motile, catalase negative, positive to produce acid and had proteolytic activity. Bekasam mozambique fish contained as much as 0.64% lactic acid and pH is 5.39. The results of the proximate analysis bekasam mozambique fish showed that the fermentation process increased the nutritional value bekasam quality mozambique fish with 5.5270% water content, 33.0628% ash content, 0.0788% crude fiber content, 45.0546% crude protein content, 7.9419% crude fat content and 13.9407% carbohydrate content. Keywords : bekasam , lactic acid bacteria , isolation, characterization, proximate analysis

BIOPROSPEKSI BAKTERI YANG BERASOSIASI DENGAN ALGA HIJAU HALIMEDA MACROLOBA, CAULERPA RACEMOSA, DAN ULVA SP SEBAGAI PENGHASIL SENYAWA ANTIBAKTERI

Jurnal Akademika Biologi Vol. 2 No. 4 Oktober 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bacteria can grow in various environments, including those associated with marine organisms such as algae, sponge, sea grass and soft corals. For these organisms, bacteria help building their defense by producing secondary metabolites such as bioactive compound.  This research aims to study the potency of the bacteria which interact with green algae Halimeda macroloba, Caulerpa racemosa, and Ulva sp which are able to produce antibacterial compound. Algae, which belong to Chlorophyta, are common to be found in marine water. This research used bacterial isolation, morphologic bacterial isolate characterization, antibacterial test, molecular identification of the antibacterial compound producer isolate, and biochemical activity test. From the isolation, the researcher was able to collect five bacterial isolate; one from H. macroloba, three from C. racemosa, and one isolate from Ulva sp. Of the five isolates, one isolate from C. racemosa can prevent the E.coli, and one isolate from H. macroloba can prevent E.coli and P.aeruginosa. Isolate derived from Halimeda macroloba have the biggest prevention zone ability, which is 18.1 mm, towards P.aeruginosa. Keywords: Antibacterial, Bacterial Association, Halimeda macroloba, Caulerpa racemosa,      Ulva sp

EFEKTIVITAS PENGAWETAN SECARA OZONISASI DAN IDENTIFIKASI TERHADAP KONTAMINASI KAPANG PADA KACANG TANAH (Arachis hypogaea L.)

Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Peanut (Arachis hypogaea L.) is a potential plant protein source with a relatively cheap price, so that it can be consumed by almost all the community. Post-harvest handling of peanuts especially at the stage of the marketing needs to be done properly, because improper treatment can lead to contamination by fungi. As a result, it can reduce the quantity and quality of the product and can endanger the health of consumers. This study aims to determine the Ozonisation Preservation and Identification of Mold Contamination In Peanuts. Mold was isolated using the direct method (direct method) and dilution. Identification of molds were done macroscopically and microscopically. Identification was done by using Potato Dextrose Agar (PDA) and Malt Extract agar (MEA) as selective media. The result of Ozonisation Preservation with 3.5 ppm concentration showed that peanuts that had been exposed with ozone on first day containing the amount of molds lower than on the day 7, 14, 21, and 28. The result of mold identification on peanuts consisted of one genus Aspergillus with predominant isolate was A. flavus as many as 25 colonies. Keywords: peanut (Arachis hypogaea L.), ozonation, identification, mold

EKSPLORASI DAN KARAKTERISASI BAKTERI POTENSIAL PENGHASIL SENYAWA ANTIFOULING YANG BERASOSIASI DENGAN ALGA COKLAT (PHAEOPHYTA) DI PERAIRAN KEPULAUAN KARIMUNJAWA JEPARA

Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Biofouling is a settlement process from marine organisms initiated by biofilm (microbial layers). Corrosion on the vessels, pipelines under the sea, oil plants, etc are big problems in the industrial today cause adversely affects. Antifouling paints have been developed to prevent settlement of organisms, however antifouling paints contain heavy metals and biocides which have toxic effects on marine ecosystems. Antifouling compound which environmental friendly is a solution for one of the paint component. The aim of this research was to isolate bacteria associated with brown algae which had potential to produce antifouling compound from Karimunjawa islands, Jepara.Isolate KS1-1 showed antifouling activity against Pseudomonas aeruginosa as biofilm-forming bacteria.This isolate had been characterized in morphology, moleculer and biochemichal identification.Moleculer analyses of 16S rRNA sequence, KS1-1 showed similarity 94% from 287 bp with Vibrio sp. W-137-16S ribosomal RNA gene, partial sequence. Keywords : Antifouling, Phaeophyta,Biofouling, 16S rRNA, Vibrio

Co-Authors AB Susanto Afrazak Johansyah Agung Suprihadi Aisyah, Arina AN, Luthfy Anto Budiharjo Anwar, Soni Nugraha Arfina, Devia Kusmawati Arina Tri Lunggani Basundari, Sinta Anas Bintoro Rudi Saputro, Bintoro Rudi Budi Raharjo Budi, Khrisna Lazuardi Christiningrum, Octavia Dewi Desi Arisanti Dian Arif Rachman Elawati, Nunung Eni Endah Dwi Hastuti Enny Yusuf Wachidah Yuniwarti Enny Yusuf Wachidah Yuniwarti Erma Prihastanti Faizah, Laila Nur Fatimah, Sona Ginting, R Cinta Badia Hadi Endrawati Hananto, Sigit Hapsari, Betalini Hermana, Nabila Swarna Puspa Hermin Hermin, Hermin Hermin P Kusumaningrum Hermin P. Kusumaningrum Hermin Pancasakti Kusumaningrum Indah Sulistyarini Isworo Rukmi JOEDORO SOEDARSONO Joedoro Sudarsono Khabib Khasan Alfaridhi, Khabib Khasan Kodhatin, Syawal Nurangga Kristyaningrum, Dwi Kurniati, Yoni Anggun Endah Larasati, Ella Dewani Larosa, Sofyan Fauzi Lestari, T. A. Lunggani, Arina Mergypta, Deverina MG Isworo Rukmi Moi, Maria Yasinta Muhammad Nur Muhammad Z ainuri Muhammad Zaenuri Muhammad Zainuri Mumtianah, Olivia Nisa Munifatul Izzati Naufal, Adhitya Ningsih, Devi Ayu Nugraha, Soni Nugroho, Risky Panji Nuraini, Neny Nurhayati Nurhayati Nurmila, Ika Oktavia Palupi, Dyah Parastiti, Darnia Astari Pardede, Lasria Prastya, M Eka Pratama, Barri Pratama, Novi Alvita Prianto, A Heru Purwaningsih, Dani Ika Putri, Adde Lolita Putri, Choirul Rizka Rachmawati, Aniza Rahmawati, Fauziah Citra Rahmiati -, Rahmiati Rejeki Siti Ferniah Rida Yuliana, Rida Rully Rahadian Sabdaningsih, Aninditia Safitri, Linda Sahlan, Ahmad Qi Saniha Adini, Saniha Sari, Anisa Rachma Sarjana Parman Sarjiya, Antonius Setiawan Wicaksono, Setiawan Siregar, Mangasa Tua Pandapotan Siti Nur Jannah Sri Pujianto, Sri Sri Pujiyanto Suryaningsih, Vivi Tri Muji Ermayanti Tri Winarni Agustini Triwibowo Yuwono Tyas Rini Saraswati Udi Tarwotjo, Udi Usman, Doni Wardani, Mia Tri Wijanarka Wijanarka Wijanarka, W Yeti Darmayati Zulfikar, Muhamad Fikri