Neng Fisheri Kurniati
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Antidiabetic Activity of Leaves Ethanol Extract Chromolaena odorata (L.) R.M. King on Induced Male Mice with Alloxan Monohydrate Marianne, Marianne; Lestari, Dwi; Sukandar, Elin Yulinah; Kurniati, Neng Fisheri; Nasution, Rosnani
Jurnal Natural Volume 14, Number 1, March 2014
Publisher : Syiah Kuala University

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Abstract

Chromolaena odorata or Tekelan has been used traditionally to cope with diabetes mellitus, but the scientific evidence as antidiabetic agent has not been previously reported. Therefore, it is necessary to test the antidiabetic activity using ethanol extract of the tekelan leaves in male mice induced by alloxan. Mice were induced intravenously by alloxan dose of 55 mg/kg bw. After 7 days, mice were diabetic ( KGD > 200 mg/dL ) and were grouped into 6 groups: negative control group (only treated with CMC - Na) , positive control group (glibenclamide 0.45 mg/kg bw) and the 4 other groups were given ethanol extract of Tekelan leaves at doses of 5 , 25 , 125 and 250 mg/kg bw. Dosage administered for 28 days and blood glucose levels was checked every 7 days using a glucotest. Data were analyzed by one-way analysis of variation at 95% confidence level. Ethanol extract of tekelan’s leaves  at doses of 5 , 25 , 125 and 250 mg/kg bw can lower blood levels  from day 7 to day 28 and was significantly different compared to the negative control group ( p < 0.05). Ethanol extract of tekelan’s leaves at doses of 5 , 25 , 125 and 250 mg/kg bw showed antidiabetic activity in alloxan-induced mice.
Aktivitas Makrofag Meningkat Pada Aorta Tikus Hiperkolesterolemia Kurniati, Neng Fisheri; Nurfatwa, Maritsa; Artarini, Aluicia Anita
Majalah Kedokteran Bandung Vol 50, No 1 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v50n1.1159

Abstract

Aterosklerosis merupakan, kondisi inflamasi kronik, faktor resiko penyakit kardiovaskular disebabkan oleh tingginya kadar kolesterol. Tujuan penelitian ini mengevaluasi peran mieloperoksidase (MPO) dan makrofag di aorta dan jantung tikus yang diinduksi hiperkolesterolemia. Penelitian ini dilakukan pada Maret–Agustus 2016 di Laboratorium Farmakologi dan Bioteknologi Institut Teknologi Bandung. Tikus dikelompokkan menjadi kelompok normal dan hiperkolesterolemia. Induksi hiperkolesterolemia dilakukan dengan pemberian pakan tinggi kolesterol, kolesterol murni, asam kolat dan propiltiourasil (KKT) selama 5 bulan. Kolesterol total diukur sebelum induksi, pertengahan, dan setelah induksi. HDL, trigliserida (TG), LDL, indeks aterogenik (IA), jumlah sel darah merah dan sel darah putih diukur setelah induksi. Deteksi ekspresi mieloperoksidase (MPO) dan CD68 pada aorta dan jantung dilakukan dengan metode dot blot dan ELISA. Induksi hiperkolesterolemia selama 5 bulan menghasilkan kadar kolesterol total (364,10±148,46 mg/dL), HDL (7,90±1,29 mg/dL), LDL (307,47±116,91 mg/dL), dan Indeks Aterogenik (1,04±0,23). Kadar kolesterol yang tinggi meningkatkan jumlah sel darah putih yang bersirkulasi namun tidak mempengaruhi jumlah sel darah merah. Jumlah makrofag yang berada di jaringan aorta dan jantung kelompok hiperkolesterolemia meningkat secara signifikan dibanding dengan kelompok normal. Namun, peningkatan aktivitas makrofag yang diukur dari ekspresi MPO hanya teramati pada aorta hewan hiperkolesterolemia, tidak pada jantung. Simpulan, aktivitas makrofag meningkat hanya pada aorta hewan hiperkolesterolemia diduga berperan dalam pembentukan plak ateroma di aorta. Kata kunci: Aorta, CD68, hiperkolesterolemia, makrofag, mieloperoksidase Macrophage Activity Increases in Hypercholesterolemia Rat AortaAtherosclerosis, which is an inflammatory chronic condition, is one of the major risk factors of cardiovascular disease caused by hypercholesterolemia. This study aimed to evaluate role of myeloperoxidase (MPO) and macrophage in aorta and heart of rat hypercholesterolemia. This research was conducted in March–August 2016 at Pharmacology and Biotechnology Laboratory of Institut Teknologi Bandung. Rats were divided into normal and hypercholesterolemia groups. Hypercholesterolemia was induced by cholesterol feeding and CCT (cholesterol, cholic acid and propiltiourasil) oral administration for 5 months. Total cholesterol was measured before induction (T0), in the middle (T2.5), and after induction (T5). HDL, triglyceride (TG), LDL, aterogenic index (IA), red blood, and white blood cell count was measured after induction (T5). Success of induction was proven by the elevation of cholesterol total value of hypercholesterolemia group compared to normal group. Myeloperoxidase (MPO) and CD68 in aorta and heart hypercholesterolemia rat was detected by dot blot and ELISA method. Hypercholesterolemia group showed significant differences in total cholesterol value (364.10±148.46 mg/dL), HDL value (7.90±1.29 mg/dL ), LDL value (307.47±116.91) and Atherogenic Index (1.04±0.23). High level of cholesterol increases circulating white blood cells but have no effect on  circulating red blood cells. Macrophage in the  hypercholesterolemia group increased significantly compared to the normal group. However, the increase in macrophage activity identified throughMPO expression was only seen in hypercholesterolemic aorta but not  in the heart. It is concluded that macrophage activities increase in the aortic tissue, but  not in the heart tissue of the hypercholesterolemia group, which may contribute to the formation of atheroma plaque in aorta. Key words: Aorta, CD68, hypercholesterolemia, macrophage, myeloperoxidase
Aktivitas Antidiare Ekstrak Etanol Daun Suji (Dracaena Angustifolia Roxb) Sukmawati, Ika Kurnia; Sukandar, Elin Yulinah; Kurniati, Neng Fisheri
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 14 No. 02 Desember 2017
Publisher : Fakultas Farmasi, Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pharmacy.v14i2.1948

Abstract

Penyakit diare masih menjadi masalah kesehatan terutama diberbagai Negara berkembang termasuk Indonesia. Secara tradisional masyarakat telah menggunakan daun Suji (Dracaena angustifoliaRoxb L.) untuk mengatasi berbagai gangguan pencernaan termasuk diare.Tujuan dari penelitian ini adalah untuk menguji aktivitas antidiare dan antibakteri ekstrak etanol daun suji. Ekstraksi dilakukan dengan metode refluks menggunakan pelarut etanol 96 %.Uji aktivitas antimikroba dilakukan secara invitro dengan menggunakan metode broth microdilution terhadap ekstrak. Mikroba uji yang digunakan adalah Escherichia coli, Shigella dysenteriae, Shigella flexneri, dan Salmonella typhi. Dilakukan uji antidiare pada hewan uji yang diinduksi minyak jarak. Sediaan uji diberikan satu jam sebelum induksi kemudian dilakukan pengamatan terhadap feses (frekuensi, konsistensi dan berat). Metode waktu lintas usus juga dilakukan pada percobaan ini dengan prinsip membandingkan usus yang dilalui marker dengan panjang usus seluruhnya. Dari pengujian antibakteri ekstrak daun suji mempunyai kemampuan penghambatan pada bakteri Shigella dysenteriae, Shigella flexneri, Eschericia coli, dan Salmonella typhi, dengan KHM berturut-turut  25000 µg/ml,  25000 µg/ml, 12500 µg/ml, dan > 25000 µg/ml. Dari hasil uji aktivitas antidiare daun suji kelompok dosis ekstrak yang mempunyai aktivitas dalam memproteksi diare oleh minyak jarak yaitu dosis 50 mg/kgbb, dosis ini dapat menurunkan frekuensi defekasi  berbeda bermakna disbanding kelompok kontrol (p<0,05) , dosis 25 mg/kgbb dan 100 mg/kgbb dapat meningkatkan konsistensi dan menurunkan berat feses.
Ekstrak Daun Binahong Mencegah Kenaikan Kolesterol Darah pada Tikus yang Diberi Pakan Lemak Tinggi Rahman, Asep Abdul; Kurniati, Neng Fisheri; Sukandar, Elin Yulinah
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 8, No 2 (2016)
Publisher : Indonesian Research Gateway

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Abstract

Binahong (Anredera cordifolia (Ten.) v. Steenis) leaves empirically have been used to lower cholesterol levels. In this study, preventive activity of ethanol extracts will be tested on the animals fed with a high-cholesterol chow. Hypercholesterolemic animal model established by orally administering high-cholesterol chow, pure cholesterol, cholic acid, and propiltiourasil. The test animals were divided into 5 groups, including the positive control group, ethanol extract of binahong leaves at doses of 50 mg/kg bw, 100 mg/kg bw, and 200 mg/kg bw; and simvastatin at dose of 36 mg/kg bw. Extract was given at the same time as hypercholesterol induction. Lipid profiles were measured at the time before the induction, 15 days and 29 days after induction. Index of atherogenic was also calculated. The results showed that level of cholesterol and LDL of extract binahong at dose of 50 mg/kg (109.17±26.60 mg/dL and 55.78±23.92 mg/dL, respectively) had significant difference compared to a positive control group (p &lt; 0.05). Furthermore, binahong extract at dose of 50 mg/kg bb had the lowest atherogenic index (0,68±0,27) and aorta thickening (150.60±41.72 μm) compared to other extracts at day 15 of induction. In conclusion, extract binahong at dose of 50 mg/kg bw rat gives the best effect to prevent the increase of cholesterol and LDL level on wistar male rats fed with a high-cholesterol chow.
Cardioprotective Potential of Ethanol Extract of Sonchus Arvensis L. Leaves on Isoproterenol-Induced Myocardial Infarction in Rat Kurniati, Neng Fisheri; Sukandar, Elin Yulinah; Pardilah, Rian; Suliska, Nova; Ayuningtyas, Dhyan Kusuma
JURNAL ILMU KEFARMASIAN INDONESIA Vol 16 No 1 (2018): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

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Abstract

Sonchus arvensis L. leaves have been used traditionally to treat various disease conditions. This study is designed to evaluate cardioprotective potential of ethanol extract of S. arvensis leaves on isoproterenol-induced myocardial infarction in Wistar rat. Male Wistar albino rats were divided into three main groups: negative control (saline only), positive control (isoproterenol only), and S. arvensis extract treated groups. S. arvensis extract was administered in three doses; 50, 100, and 200 mg/kg b.w. p.o for 14 days. On day 13 and 14, isoproterenol (85 mg/kg bw) was given intraperitoneally to positive control and extract treated groups. The parameters studied were cardiac biomarker enzymes which were Creatine Kinase (CK), Creatine Kinase-MB (CK-MB), Aspartate Transaminase (AST), Alanine Transaminase (ALT) and Lactate Dehydrogenase (LDH). The results showed that S. arvensis at dose of 50 mg/kg b.w. could significantly (P&lt;0.05) reduce the level of CK, CK-MB, AST, ALT, and LDH in myocardial infarcted rats compared to positive control. The increase of the dose of S. arvensis extract was not followed by an increase of its cardioprotective activity. In conclusion, Sonchus arvensis L. leaves extract at dose of 50 mg/kg b.w. has potential to be developed as cardioprotective drug.
Aktivitas Makrofag Meningkat Pada Aorta Tikus Hiperkolesterolemia Kurniati, Neng Fisheri; Nurfatwa, Maritsa; Artarini, Aluicia Anita
Majalah Kedokteran Bandung Vol 50, No 1 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v50n1.1159

Abstract

Aterosklerosis merupakan, kondisi inflamasi kronik, faktor resiko penyakit kardiovaskular disebabkan oleh tingginya kadar kolesterol. Tujuan penelitian ini mengevaluasi peran mieloperoksidase (MPO) dan makrofag di aorta dan jantung tikus yang diinduksi hiperkolesterolemia. Penelitian ini dilakukan pada Maret–Agustus 2016 di Laboratorium Farmakologi dan Bioteknologi Institut Teknologi Bandung. Tikus dikelompokkan menjadi kelompok normal dan hiperkolesterolemia. Induksi hiperkolesterolemia dilakukan dengan pemberian pakan tinggi kolesterol, kolesterol murni, asam kolat dan propiltiourasil (KKT) selama 5 bulan. Kolesterol total diukur sebelum induksi, pertengahan, dan setelah induksi. HDL, trigliserida (TG), LDL, indeks aterogenik (IA), jumlah sel darah merah dan sel darah putih diukur setelah induksi. Deteksi ekspresi mieloperoksidase (MPO) dan CD68 pada aorta dan jantung dilakukan dengan metode dot blot dan ELISA. Induksi hiperkolesterolemia selama 5 bulan menghasilkan kadar kolesterol total (364,10±148,46 mg/dL), HDL (7,90±1,29 mg/dL), LDL (307,47±116,91 mg/dL), dan Indeks Aterogenik (1,04±0,23). Kadar kolesterol yang tinggi meningkatkan jumlah sel darah putih yang bersirkulasi namun tidak mempengaruhi jumlah sel darah merah. Jumlah makrofag yang berada di jaringan aorta dan jantung kelompok hiperkolesterolemia meningkat secara signifikan dibanding dengan kelompok normal. Namun, peningkatan aktivitas makrofag yang diukur dari ekspresi MPO hanya teramati pada aorta hewan hiperkolesterolemia, tidak pada jantung. Simpulan, aktivitas makrofag meningkat hanya pada aorta hewan hiperkolesterolemia diduga berperan dalam pembentukan plak ateroma di aorta. Kata kunci: Aorta, CD68, hiperkolesterolemia, makrofag, mieloperoksidase Macrophage Activity Increases in Hypercholesterolemia Rat AortaAtherosclerosis, which is an inflammatory chronic condition, is one of the major risk factors of cardiovascular disease caused by hypercholesterolemia. This study aimed to evaluate role of myeloperoxidase (MPO) and macrophage in aorta and heart of rat hypercholesterolemia. This research was conducted in March–August 2016 at Pharmacology and Biotechnology Laboratory of Institut Teknologi Bandung. Rats were divided into normal and hypercholesterolemia groups. Hypercholesterolemia was induced by cholesterol feeding and CCT (cholesterol, cholic acid and propiltiourasil) oral administration for 5 months. Total cholesterol was measured before induction (T0), in the middle (T2.5), and after induction (T5). HDL, triglyceride (TG), LDL, aterogenic index (IA), red blood, and white blood cell count was measured after induction (T5). Success of induction was proven by the elevation of cholesterol total value of hypercholesterolemia group compared to normal group. Myeloperoxidase (MPO) and CD68 in aorta and heart hypercholesterolemia rat was detected by dot blot and ELISA method. Hypercholesterolemia group showed significant differences in total cholesterol value (364.10±148.46 mg/dL), HDL value (7.90±1.29 mg/dL ), LDL value (307.47±116.91) and Atherogenic Index (1.04±0.23). High level of cholesterol increases circulating white blood cells but have no effect on  circulating red blood cells. Macrophage in the  hypercholesterolemia group increased significantly compared to the normal group. However, the increase in macrophage activity identified throughMPO expression was only seen in hypercholesterolemic aorta but not  in the heart. It is concluded that macrophage activities increase in the aortic tissue, but  not in the heart tissue of the hypercholesterolemia group, which may contribute to the formation of atheroma plaque in aorta. Key words: Aorta, CD68, hypercholesterolemia, macrophage, myeloperoxidase