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Studi Lesi Makroskopis dan Mikroskopis Embrio Ayam yang Diinfeksi Virus Newcastle Disease Isolat Lapang yang Virulen Putra, Hamdu Hamjaya; Wibowo, Haryadi; Untari, Tri; Kurniasih, Kurniasih
Jurnal Sain Veteriner Vol 30, No 1 (2012)
Publisher : Fakultas Kedokteran Hewan

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Abstract

Newcastle disease (ND) disebabkan oleh Avian paramyxovirus dari keluarga Paramyxoviridae, merupakan salah satu penyakit utama pada ayam. Penelitian ini bertujuan untuk mengetahui lesi pada organembrio ayam secara makroskopis maupun mikroskopis yang diinfeksi oleh virus ND. Telur ayam berembrio (TAB) diinokulasi oleh virus ND Salatiga dan virus ND La Sota. Aquabidestilata digunakan sebagai kontrolnegatif. TAB yang menunjukkan kematian embrio disimpan di refrigerator, kemudian dikoleksi cairan allantoisnya. Embrio ayam yang mati dilakukan pengamatan secara makroskopis. Organ dari embrio ayam dibuat preparat histopatologi dengan pewarnaan Hematoxylin dan Eosin (H&E) untuk pemeriksaan mikroskopis. Identifikasi adanya pertumbuhan virus ND pada isolat dilakukan dengan uji hemaglutinasi dan uji hemaglutinasi inhibisi menggunakan serum anti ND. Embrio ayam yang diinfeksi oleh virus ND Salatiga mengalami kematian kurang lebih 26 jam pasca inokulasi. Lesi makroskopis yang teramati berupa hemoragipada kulit. Lesi mikroskopis menunjukkan adanya kongesti dan hemoragi pada paru-paru, kongesti dan radang pada kulit, serta kongesti pada usus, hati, ginjal, dan jantung. Embrio ayam yang diinfeksi virus ND La Sota secara makroskopis teramati kongesti ringan pada kulit. Lesi mikroskopisnya menunjukkan adanya kongesti pada paru-paru, kongesti dan radang pada kulit, serta kongesti pada hati, ginjal, dan jantung. Lesi makroskopis dan mikroskopis embrio ayam yang diinfeksi virus ND Salatiga lebih parah bila dibandingkan dengan lesi akibat virus ND La Sota.Kata kunci: Newcastle disease, embrio ayam, lesi makroskopis, lesi mikroskopis, La Sota
Gambaran Histopatologi Otak Tikus Akibat Injeksi Trimetyltin sebagai Model Penyakit Alzheimer Kristianingrum, Yuli Purwandari; Sitarina Widyarini, Sitarina Widyarini; Kurniasih, Kurniasih; Bambang Sutrisno, Bambang Sutrisno; Charles Rangga Tabbu, Charles Rangga Tabbu Charles Rangga Tabbu; Sugiyono, Sugiyono
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.22146/jsv.22820

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Trimethyltin chloride (TMT) is an organotin compound which neurotoxic at limbus system and hippocampus in human and animal. Pathology changes that caused by the induction of TMT is a neurodegenerative disorder such as nerve cell death and cognitive impairment. This study was aimed to observe brain pathology induced by TMT with multiple doses for 14, 21 and 28 days after treatment. Twenty seven of Wistar rats, at 2 months of age with weight ranging between 200-300 grams were used and divided randomly into 3 groups (n=9). Group I were injected by trimetyiltin with a dose of 6 mg / kg, group II were injected bytrimetyltin with a dose of 8 mg / kg and group III as control without injection. Observation of brain pathology was done by euthanasia on day 14, 21 and 28 after treatment, three rats each. Cortex and hippocampus of the brainwere observed using Hematoxilin and Eosin staining (HE). All of the research procedure was done with the approval and supervision of Animal Ethics Committee LPPT UGM No. 300/KEC-LPPT/VII/2015. The observation of histopathology of the brains neuron cells injected by trimetyltin dose of 6 mg/kg and 8 mg/kg body weight was showed increasing cell death of brain neurons in the cortex and hippocampus compared to the control group. The highest cell death was on day 14 in the hippocampus and cortex cerebral on day 21after TMT injection. The neuron cell death characterized by the shrink of brain neurons as well as colored eosinophilic cytoplasm. One way ANOVA statistical analysis showed a significant difference number of neurons cell deathbetween control and treatment groups. Based on this research, it can be concluded that the trimetyltin injection dose of 6 mg / kg and 8 mg / kg of body weight caused neuron cell death in the brain rats from fourteen day aftertreatment, especially in the hippocampus and cortex.
The Intellectual Capital Effect on Financial Performances at Islamic Insurance Syah Aji, Rizqon Halal; Kurniasih, Kurniasih
Al-Iqtishad: Journal of Islamic Economics Vol 7, No 2: July 2015
Publisher : Faculty of Shariah and Law

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.875 KB) | DOI: 10.15408/aiq.v7i2.1696

Abstract

The research has analyzed the intellectual capital effect of the Islamic insurance company’s financial performance. Intellectual capital is quantified by VAICTM (Value Added Intellectual Capital), for efficiency components are physical capital coefficient (VACA), human capital coefficient (VAHU), and structural capital coefficient (STVA). Financial performance is quantified by ROA and RBC. Data is given from seven Islamic insurance companies during 2009-2013. Data analysis used is PLS (Partial Least Square). The result shows that intellectual capital factors had an affect the company’s financial performance. Intellectual capital relation to financial performance parameter value estimated coefficient 0,845 with t-statistic 46,771. VAICTM enables to elucidate financial performance variable 71, 6%, is by finding the R-square PERF value (financial performance) 0,716.DOI: 10.15408/aiq.v7i2.1696
THE EFFECT OF APUS BAMBOO (Gigantochloa apus) LEAVES INFUSION TO MORTALITY RATE AND MORPHOMETRY OF Haemonchus contortus ADULT WORM IN VITRO Widiarso, Budi Purwo; Nurcahyo, Wisnu; Kurniasih, Kurniasih; Prastowo, Joko
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.488 KB) | DOI: 10.21157/j.ked.hewan.v11i4.8167

Abstract

This present project was carried out to determine the effect of apus bamboo (Gigantochloaapus )leaves infusion to mortality rate and morphometry of Haemonchus contortus adult worms in vitro. The formulated tools were collected through camera, 3 ml syringe, object glass, deck glass, mortar, drip dropper, waterbath, microscope, stopwatch, counter check, oven, erlenmeyer flask, electric scale, surgical scissor, and pipette. The allocated materials used in this studywere bamboo leaves infusion, Haemonchus contortus adult worms, aquadestilata, ethanol and NaCl 0.62%. The research was firstly concernedwith making 0.1% and 1% apus bamboo leaves infusion. The samples were obtained from Haemonchus contortus collection. Adult worms were obtained directly from abomasum of naturally infected goats that were cut at Animal Slaughter House (RPH). To investigate the matter, mortality rates of adult worms at various doses and times of observation as well as differences in Haemonchus contortus morphometry were analyzed using ANOVA. The findings disclosed that apus bamboo leaves infusion demonstrated a significant difference in the mortality rate of adult worms Haemonchus contortusat various doses and times of observation. This report presented the findings of research that the best dose to increase the mortality of worms was 1% bamboo leaves infusion. Moreover, apus bamboo leaves infusion affected the morphometry of Haemonchus contortus adult worms, especially in body length, cervical papilla width, and spicules length in males, and body length, cervical papilla width, and vulvar length in females.   
HISTOPATHOLOGICAL CHANGES OF SCHISTOSOMIASIS FROM NAPU VALLEY, CENTRAL SULAWESI, 1NDONESIA Kurniasih, Kurniasih
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan

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Abstract

Schistosomiasis is an endemic disease caused by the infection Schistosoma Japonicum (Trematoda), can attack human and animals surrounding Lindu lake and Napu valley. Central Sulawesi, Indonesia. Five mice were naturally infected through subcuian with 75 cercariae of Schistosoma japonicum emerged from snail, Oncomelanic hupensis lindoensis, which were found in the Napu valley, Central Sulawesi. Mice showed a weakness, anorrhexia, stomach ache,and died in the fifth, sixth and seventh week posiinfection. At autopsy, mice had ascites, yellowish nodules on the surface of lung, liver, serosa of the small intestine, and mesenterium. Histolagically, there were severe necrosis and the granolomatous inflammation of the liver, the spleen, the lung, the pancreas, and the small intestine.
The Distribution of Microalgae in a Stabilization Pond System of a Domestic Wastewater Treatment Plant in a Tropical Environment (Case Study: Bojongsoang Wastewater Treatment Plant) Ariesyady, Herto Dwi; Fadilah, Rifka; Kurniasih, Kurniasih; Sulaeman, Aminudin; Kardena, Edwan
Journal of Engineering and Technological Sciences Vol 48, No 1 (2016)
Publisher : ITB Journal Publisher, LPPM ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.306 KB) | DOI: 10.5614/j.eng.technol.sci.2016.48.1.7

Abstract

The Bojongsoang Wastewater Treatment Plant (WWTP) serves to treat domestic wastewater originating from Bandung City, West Java, Indonesia. An abundant amount of nutrients as a result of waste decomposition increases the number of microalgae populations present in the pond of the wastewater treatment plant, thereby causing a population explosion of microalgae, also called algal blooming. In a stabilization pond system, the presence of algal blooming is not desirable because it can decrease wastewater treatment performance. More knowledge about the relationship between the nutrients concentration and algae blooming conditions, such as microalgae diversity, is needed to control and maintain the performance of the wastewater treatment plant. Therefore this study was conducted, in order to reveal the diversity of microalgae in the stabilization pond system and its relationship with the water characteristics of the comprising ponds. The results showed that the water quality in the stabilization pond system of Bojongsoang WWTP supported rapid growth of microalgae, where most rapid microbial growth occurred in the anaerobic pond. The microalgae diversity in the stabilization ponds was very high, with various morphologies, probably affiliated with blue-green algae, green algae, cryptophytes, dinoflagellates and diatoms. This study has successfully produced information on microalgae diversity and abundance profiles in a stabilization pond system.
PERBEDAAN HASIL BELAJAR GEOGRAFI ANTARA MEDIA POWER POINT DAN MEDIA CHART PADA MATERI HIDROSFER KELAS X SEMESTER GENAP TAHUN AJARAN 2012/2013 SMA NEGERI 1 BANJARHARJO, BREBES Kurniasih, Kurniasih
Edu Geography Vol 3 No 4 (2015)
Publisher : Edu Geography

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Abstract

Penelitian bertujuan: 1) Untuk mengetahui hasil belajar geografi materi hidrosfer yang pembelajarannya menggunakan media power point 2) Untuk mengetahui hasil belajar geografi materi hidrosfer yang pembelajarannya menggunakan media chart 3) Untuk mengetahui perbedaan hasil belajar geografi materi hidrosfer antara pembelajaran yang menggunakan media power point dengan media chart. Populasi penelitian yaitu siswa kelas X SMA Negeri 1 Banjarharjo. Teknik pengambilan sampel menggunakan purposive random sampling. Variabel penelitian: 1) Hasil belajar siswa materi hidrosfer menggunakan media pembelajaran power point. 2) Hasil belajar siswa materi hidrosfer menggunakan media pembelajaran chart. Teknik analisis data yang digunakan yaitu menggunakan analisis deskriptif persentatif dan uji statistik uji-t. Hasil penelitian menunjukkan: 1) Hasil belajar menggunakan media power point nilai rata-rata kognitif 80,65, sedangkan nilai rata- rata afektif pertemuan I 82,6, pertemuan II 85,1, dan pertemuan III 88,2. 2) Hasil belajar menggunakan media chart nilai rata-rata kognitif 72,60, sedangkan rata-rata nilai afektif pertemuan I 71,5, pertemuan II 76,9, dan pertemuan III 83,1. 3) Uji perbedaan diketahui bahwa t_hitung 4,095  dan  ttabel 1,67, taraf signifikasi 5% dan dk= 33 + 33 – 2 = 64, karena thitung > ttabel sehingga Ho ditolak dan Ha diterima. Research aims: 1) To assess the learning outcomes of the hydrosphere geography learning materials using medium power point  2) To know the result of that study geography hydrosphere learning material using a media chart 3) To determine differences in learning outcomes between the hydrosphere geography teaching materials using the media power point with media charts. The study population are students of class X SMA Negeri 1 Banjarharjo, Purposive sampling technique using random sampling. Research variables: 1) Student learning outcomes hydrosphere material using instructional media power point. 2) Student learning outcomes hydrosphere material using instructional media charts. Data analysis techniques used are persentatif using descriptive analysis and t-test statistical test. The results showed: 1) The results of learning to use the media power point average cognitive score of 80,65, while the average value of 82,6 affective first meeting, the meeting II 85,1, and meeting III 88,2. 2) The result of learning to use the media charts the average value of cognitive 72,60, while the average value of 71,5 affective first meeting, the meeting II 76,9, and meeting III 83,1. 3) Test the difference is know that t-hitung 4.095 and t-tabel 1.67, 5% significance level and df = 33 + 33 – 2 = 64, because of t-hitung > t-table so that Ho is rejected and Ha sccepted.
Lactic Acid Bacteria Isolated from the Gastro-Intestinal Tract of Chicken: Potential Use as Probiotic Harimurti, S; Nasroedin, ES; Nasroedin, Nasroedin; Kurniasih, Kurniasih
ANIMAL PRODUCTION Vol 9, No 2 (2007): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Lactic acid bacteria have been suggested to have several beneficial effects on human and animals.  These bacteria, indigenous to the gastro-intestinal tract, are important in regulating the balance among the desirable and undesirable intestinal microflora and in controlling enteric pathogenic infection in the host.  Objectives of this research are to obtain lactic acid bacteria isolates from gastro-intestinal tract of chicken and to screen their ability as a probiotic agent i.e., their antagonistic against pathogenic bacteria, their survival at low pH and high concentration of bile salt.  In this research, 74 samples used as sources of bacteria, and among them only 11 samples could be isolated as lactic acid bacteria with the total number of isolates of 61.  Based on the preliminary screening i.e., their antagonistic factor against pathogenic bacteria, 20 isolates was further studied.   Based on the identification scheme, these isolates belong to three species, i.e., Lactobacillus murinus, Pediococcus acidilactici, and Streptococcus thermophilus. The result showed that most isolates grow well in the media with the initial pH of 5.5, but their growth were retarded when the initial pH 3.5.  Only one isolate Streptococcus thermophilus Kp-2 showed its growth at initial pH of 3.5.   All isolates did not show any growth at initial pH 2.5, though their viability still high. The result based on the isolates resistance to bile salt showed that most isolates could grow at media with 0.20% of bile salt.  Their growth was inhibited with the increasing bile salt concentration.  However, few isolates could grow well at media with 1% of bile salt. Based on their characteristics three isolates i.e., Lactobacillus murinus Ar-3, Streptococcus thermophilus Kp-2, and Pediococcus acidilactici Kd-6 were selected as probiotic agents for the continuing research. i.e. production of biomass and its application to chicken production. (Animal Production 9(2): 82-91 (2007) Key Words : Lactic acid bacteria, gastro- intestinal tract of chicken, probiotic agents
Identification of Excretory – Secretory Sporozoite Antigens of Eimeria tenella with Monoclonal Antibody Prastowo, J; Nurcahyo, W; Kurniasih, Kurniasih; Wasito, R
ANIMAL PRODUCTION Vol 7, No 2 (2005): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The research done specifically to identified the. Eimeria tenella excretory-secretory sporozoit antigen by using the monoclonal antibody reaction instead. Eimeria tenella excretory-secretory sporozoit antigen. The 107 sporozoits were obtained from existation of 5x107 oocysts with 25 ml tripsin and sodium taurocholat. Excretion - secretion antigen were preparated by freezing and thawing and then be isolated with ice bath 20 second, 20 meter of amplitude as the further treatment.  Determinating the excretion -secretion sporozoit protein by using BCA-test. While the visualization sporozoit protein antigen by SDS-PAGE. Monoclonal antibody were produced through hybridization between B BALB/c limphosit immunized with Eimeria tenella excretion-secretion sporozoite antigen and myeloma cells. The monoclonal antibody identification  of excretion – secretion antigen were done through western blott. The visualization of protein molecule weight of Eimeria tenella sporozoit were resulted 20 protein fraction, included 14, 16, 17, 18, 20, 24, 26, 32, 34, 36, 39, 43, 47, 56, 65, 67, 76, 87, 94, and 96 KDa. There were 12 kinds of monoclonal antibody could be produced from these proteins. While the 5 hybridoma immunoblotting resulted specific reaction by the appearances of reaction ribbon obviously, there were MABset 1 which identified protein epitop with 14.7 KDa of molecule weight,  MAbset 2 which identified protein epitop with 43 KDa of molecule weight, MAbset 3 which identified protein epitop with 42 KDa of molecule weight, MAbset 4 which identified protein epitop with 47 KDa of molecule weight and MAbset 5 which identified protein epitop with 90 KDa of molecule weight. As the conclusion, five of excretion-secretion sporozoit antigen were proper immunogen to stimulate the hospest immunity. (Animal Production 7(2): 95-100 (2005) Key Words: Sporozoit, Antigent, Eimeria tenella, Coccidiosis
IMMUNODIAGNOSIS INFEKSI Aeromonas hydrophila PADA IKAN Kristianingrum, Yuli Purwandari; Widyarini, Sitarina; Kurniasih, Kurniasih; Sutrisno, Bambang; Tabbu, Charles Rangga; Sugiyono, Sugiyono
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.22146/jsv.38858

Abstract

Aeromonas hydrophila causes a disease that often infects fish and is known as Motile Aeromonas Septicaemia (MAS), Hemorrhagi Septisemia, Ulcer disease or Red-Sore disease. The   aims of this study were to develop polyclonal antibody of  Aeromonas hydrophila in the rabbits to   confirm the diagnosis of Aeromonas hydrophila  in the fish by immunohistochemistry staining method. Preparation of polyclonal antibodies was performed on the rabbits used to Aeromonas hydrophila bacteria that have been tested biochemically by intravenous and intraperitoneal injection. Doses of Aeromonas hydrophila  bacteria were 109 CPU/ml  of 0.5 ml at first injection, 1 ml at second injection, 2 ml at thirth injection and 3 ml at fourth injection. Blood serum collection was performed at week 5 after injection from  an  ear and intracardial vein. The result of antibody titer was 28 = 1024 which measured by   tube test. Furthermore, polyclonal   antibody was used to immunohistochemistry  staining with 400x dilution. The results of the staining showed that an immunopositive reaction in the liver, skin,lien,  gill, kidney, and heart of fish to Aeromonas hydrophila antibody. The research conclution was polyclonal antibody from rabbit can be used to accurately confirm the diagnosis of Aeromonas hydrophila  based on antigen and antibody reaction.