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Journal : Jurnal Ilmu dan Teknologi Kelautan Tropis

MORPHOLOGICAL CHARACTER AND MOLECULAR IDENTIFICATION WITH COI GENE MARKER OF HORSESHOE CRABS (Tachypleus gigas) AT COASTAL WATERS OF NORTHERN JAVA ISLAND Meilana, Lusita; Wardiatno, Yusli; Butet, Nurlisa A; Krisanti, Majariana
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 8, No 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.29244/jitkt.v8i1.12651

Abstract

The existence of horseshoe crabs Tachypleus gigas is currently on the threatened condition and its population status is also unclear. The objectives of this research were to asses the horseshoe crabs morphological characters and to identify based on molecular marker of Cytochrome Oxydase Subunit I (COI) gene. The samples were collected from five locations (Segara Menyan Subang, Ujung Kulon Banten, Tambak Lorok Semarang, Betah Walang Demak, and Kenjeran Surabaya) in October 2014 until June 2015. A total of 27 parameters for 62 individuals were used to determine connectivity and character identifier among populations. Data were tested using non-parametric analysis. While, the 5 individuals from each sites were used for molecular analysis including several stages of the DNA extraction, DNA electrophoresis, amplification and visualization of DNA fragments, and DNA sequencing. The results obtained based on morphological characters that T. gigas of Surabaya formed a unique group due to its body size (X1-X2 and X4-X16) was larger than the other 5 body parts (X22, X23, X26-X28) of Semarang, Demak, Subang, and Banten. According to molecular results, there were 650 bp conserve nucleotide sequences. Analysis using software MEGA 5.0 showed that there were 73 existence of specific nucleotide sites that can be used to distinguishes between T. gigas in-groups  and out-groups (T. gigas from Central Arabian Sea, T. tridentatus from Sweden, and Carcinoscorpius rotundicauda from USA).Keywords: horseshoe crabs, Java coast, morphology, identification molecular, CO1 gene.
HUBUNGAN ANTARA KETERSEDIAAN CAHAYA MATAHARI DAN KONSENTRASI PIGMEN FOTOSINTETIK DI PERAIRAN SELAT BALI Fauziah, Anna; Bengen, Dietriech G.; Kawaroe, Mujizat; Effendi, Hefni; Krisanti, Majariana
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 11, No 1 (2019): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.29244/jitkt.v11i1.23108

Abstract

ABSTRAKCahaya matahari merupakan salah satu faktor lingkungan terpenting baik secara langsung maupun tidak langsung bagi organisme fotosintetik perairan dalam menyediakan energi untuk diubah menjadi energi kimia dengan bantuan klorofil. Penelitian ini bertujuan untuk mengkaji hubungan antara ketersediaan cahaya matahari  dan konsentrasi pigmen fotosintetik di Perairan Selat Bali. Penelitian dilaksanakan pada pagi, siang dan sore hari pada lima stasiun penelitian di Perairan Selat Bali. Analisis korelasi kanonik digunakan untuk mengetahui hubungan antara ketersediaan cahaya matahari dan pembentukan pigmen fotosintetik. Hasil penelitian menunjukkan bahwa intensitas cahaya matahari (Y2) lebih berpengaruh terhadap pembentukan pigmen klorofil-b (Y1) dengan korelasi kanonik sebesar 0,4512 bilamana dibandingkan dengan pembentukan pigmen klorofil-a (Y1) dengan nilai korelasi sebesar 0,3982. Semakin tinggi pembentukan pigmen klorofil-b (Y1) dapat meningkatkan pembentukan pigmen karotenoid (Y1) secara signifikan dengan nilai korelasi kanonik sebesar 0,7419. Kesimpulan dari hasil ini, bahwa pigmen klorofil-b dan pigmen karotenoid akan terbentuk secara optimum pada intensitas cahaya rendah. ABSTRACTSunlight is one of the most important environmental factors both directly and indirectly for  photosynthetic organisms in providing energy to be converted into chemical energy with the help of chlorophyll. This study aims to examine the relationship between the availability of sunlight and the concentration of photosynthetic pigments in Bali Strait waters. The study was conducted in the morning, midday and afternoon at five research stations in the Bali Strait waters. Canonical correlation analysis was used to determine the relationship between sunlight availability and photosynthetic pigment formation. The results showed that the intensity of sunlight (Y2) had more influence on the formation of chlorophyll-b (Y1) pigments with canonical correlation of 0.4512 when compared with the formation of chlorophyll-a (Y1) pigment with a correlation value of 0.3982. The higher formation of chlorophyll-b (Y1) pigments can significantly increase the formation of carotenoid pigments (Y1) with canonical correlation values of 0.7419. From these results it can be concluded that chlorophyll-b pigments and carotenoid pigments will be formed optimally at low light intensities.
LINK OF Pharella acutidens ABUNDANCE AND MANGROVE HABITAT IN CEMPI BAY, DOMPU REGENCY, WEST NUSA TENGGAR Dermawan, Awan; Setyobudiandi, Isdradjat; Krisanti, Majariana
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 8, No 2 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.29244/jitkt.v8i2.15821

Abstract

Pharella acutidens clam is a bivalve lives in mangrove ecosystem substrate, that is one of protein source for Dompu?s people. the existence of pharella acutidens in Cempi Bay, Dompu begin difficult to collect due to mangrove area has been reduced by result of land conversion from mangrove ekosistem area to estuary aquaculture pond and mangrove timber exploitation by local community as well as the Pharella acutidens routin exploitation, these factor may has influenced the existence of Pharella acutidens clam in the Cempi Bay mangrove ecosystem. This study aims to determine the condition of the Pharella acutidens clams located in the mangrove ecosystem Cempi bay, which in is an abundance on different conditions mangrove vegetation an abundance of Pharella acutidens clams tended more on high density and high basal area sites as well as the size of the clams. Varied habitats, from the density of 1.333 trees/ha up to 3.300 trees/ha, the basal area and the diverse species of mangrove, making this study an interesting thing  This journal is part of the research that explains positive influence between total basal area of mangrove vegetation (?Ci) and abundance of Pharella acutidens, by simple regression analysis. The result showed that total basal area of mangrove vegetation (?Ci) positively influencing abundance of P. acutidens clams with equation y = 0,3038x + 3,887 and R2 = 0,9579.