Articles

Found 37 Documents
Search

PREPARASI DAN KARAKTERISASI KITIN YANG TERKANDUNG DALAM EKSOSKELETON KUMBANG TANDUK RHINOCEROS BEETLE (Xylotrupes gideon L) DAN KUTU BERAS (Sitophilus oryzae L) Komariah, Komariah; Astuti, Luki
Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.139 KB)

Abstract

ABSTRAK   Penelitian ini bertujuan mengkarakterisasi kitin hasil preparasi dari eksoskeleton kumbang tanduk Rhinoceros Beetle (Xylotrupes gideon L) dan kutu beras (Sitophilus oryzae). Penelitian terbagi menjadi penelitian pendahuluan yaitu preparasi dan uji proksimat, dan penelitian utama yang terdiri dari proses pembuatan dan karakterisasi kitin. Proses pembuatan kitin diawali dengan uji demineralisasi (HCL 1 N, 90°C), deproteinisasi (NaOH 3 N, 90°C), dan dekalorisasi (NaOCl 4%, suhu kamar). Karakterisasi meliputi tekstur, rendemen, kelarutan, kadar air, kadar abu, kadar nitrogen, kadar mineral dan derajat deasetilasi. Kadar abu dan kadar air ditentukan dengan gravimetri, kadar protein dengan kjedahl. Uji Kelarutan dengan asam asetat 2%, kadar mineral dengan spektrofotometer, sedangkan derajat deasetilasi dianalisis dengan menggunakan First Derivative Ultra Violet Spektrofotometry. Berdasarkan hasil perhitungan memperlihatkan karakteristik kitin dari eksoskeleton Rhinoceros Beetle (Xylotrupes gideon) menghasilkan kadar abu 0,98%, kadar air 8,37%, kadar nitrogen 3,02%, derajat deasetilasi 44,84%. Karakteristik kitin dari eksoskeleton Sitophilus oryzae menghasilkan kadar abu 2,00%, kadar air 8,00%, kadar nitrogen 3,57%, derajat deasetilasi 28,60 %. Kitin dari kedua sempel tidak larut dalam asam asetat 2%, berwarna putih dan tidak berbau. Hasil yang diperoleh sesuai dengan kriteria mutu kitin.   Kata Kunci: kitin, kadar air, kadar abu, kadar nitrogen, kelarutan dan derajat deasetilasi.
EFEKTIVITAS KITOSAN DENGAN DERAJAT DEASETILASI DAN KONSENTRASI BERBEDA DALAM MENGHAMBAT PERTUMBUHAN BAKTERI GRAM NEGATIF (Pseudomonas aeruginosa) DAN GRAM POSITIF (Staphylococcus aureus) RONGGA MULUT Komariah, Komariah; Wulansari, Noviana; Harmayanti, Wahyu
Prosiding Seminar Biologi Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (553.896 KB)

Abstract

Kitosan adalah derivat deasetil dari biopolisakarida kitin yang  merupakan bioplimer kedua yang melimpah di alam setelah selulosa, dan dapat ditemukan pada eksoskeleton krustasea dan serangga. Kitosan bersifat biokompabilitas, biodegradabilitas, dan tidak beracun, dan memiliki  aktivitas antimikroba yang banyak diaplikasikan  diberbagai bidang seperti industri makanan, industri tekstil, kosmetik, kedokteran dan kedokteran gigi.  Pseudomonas aeruginosa  merupakan bakteri gram negatif penyebab utama infeksi nosokomial dan sering ditemukan di saluran air pada dental unit.    P. aeruginosa  bersifat patogen dan resisten terhadap beberapa bahan antibakteri  yang ada. Staphylococcus aureus merupakan  bakteri gram positif, yang dapat menyebabkan infeksi fasial, periapikal atau periodontal abses dan  denture sore mouth. Penelitian ini dilakukan untuk mengetahui kemampuan kitosan dengan  derajat deasetilasi (DD) dan konsentrasi berbeda dalam menghambat  pertumbuhan     P. aeruginosa   dan  S.  aureus  dalam rongga mulut. Pada penelitian ini digunakan metode difusi cakram, kitosan dengan derajat deasetilasi  89% dan 93%  dimasukkan ke dalam   paper disk   dengan   konsentrasi 0,25%, 0,5%, 0,75%, 1%, 1,25% selama 24 jam dalam suhu 37oC . Zona hambat  yang terbentuk diukur dengan jangka sorong. Hasil penelitian memperlihatkan kitosan dengan derajat deasetilasi  dan konsentrasi  berbeda memberikan zona hambat yang berbeda baik pada  P. aeruginosa  dan  S. aureus. Zona hambat  P. aeruginosa   dan  S. aureus   terbesar terbentuk pada konsetrasi 1%, sedangkan kitosan dengan DD 93% memperlihatkan zona hambat lebih besar dibandingkan dengan kitosan DD 89%.   Kata Kunci : Kitosan, Derajat Deasetilasi, Psedomonas aeruginosa, Staphylococcus aureus
KARAKTERISASI KITIN DAN KITOSAN YANG TERKANDUNG DALAM EKSOSKELETON KUTU BERAS (Sitophilus oryzae) Komariah, Komariah
Prosiding Seminar Biologi Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (697.203 KB)

Abstract

Penelitian ini bertujuan mengkarakterisasi kitin dan kitosan hasil preparasi dari eksoskeleton kutu beras (Sitophilus oryzae). Penelitian terbagi menjadi penelitian pendahuluan yaitu preparasi dan uji proksimat, dan penelitian utama yang terdiri dari proses pembuatan dan karakterisasi kitin dan kitosan. Proses pembuatan kitin/kitosan diawali dengan uji demineralisasi (HCL 1 N, 90C), deproteinisasi (NaOH 3 N, 90C), dekalorisasi (NaOCl 4%, suhu kamar) dan deasetilasi (NaOH 50%, 130C). Karakterisasi meliputi tekstur, rendemen, kelarutan, kadar air, kadar abu, kadar nitrogen, kadar mineral dan derajat deasetilasi. Kadar abu dan kadar air ditentukan dengan gravimetri, kadar protein dengan kjedahl. Uji Kelarutan dengan asam asetat 2%, kadar mineral dengan spektrofotometer, sedangkan derajat deasetilasi dianalisis dengan menggunakan  First Derivative Ultra Violet Spektrofotometry. Berdasarkan hasil perhitungan memperlihatkan karakteristik kitin dari eksoskeleton Sitophilus oryzae menghasilkan kadar abu 2,00%, kadar air 8,00%, kadar nitrogen 3,57%, derajat deasetilasi 28,60 %. Kitin bersifat  tidak larut dalam asam asetat 2%, berwarna putih dan tidak berbau. Hasil yang diperoleh sesuai dengan kriteria mutu kitin. Untuk karakterisasi kitosan dari eksoskeleton kutu beras menghasilkan kadar abu 2,00%, kadar air 12,00%, kadar nitrogen 3,64%, derajat deasetilasi 68,25 %. Hasil yang diperoleh belum sesuai dengan kriteria mutu kitosan. Kata kunci :  Kutu Beras, Kitin, Kitosan, Derajat Deasetilasi
Pemberian Asam Valproat pada Induk Tikus Bunting Menghambat Sintesis Insulin pada Sel Otak Anak Tikus Komariah, Komariah; Kiranadi, Bambang; Winanto, Adi; Manalu, Wasmen; Handharyani, Ekowati
Majalah Kedokteran Bandung Vol 49, No 3 (2017)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (713.616 KB) | DOI: 10.15395/mkb.v49n3.1119

Abstract

Asam valproat memengaruhi aktivitas histone deacetylase yang berperan dalam ekspresi gen selama organogenesis. Insulin berperan dalam proliferasi dan diferensiasi sel-sel saraf dentate gyrus hipokampus. Penelitian ini bertujuan mempelajari pengaruh pemaparan asam valproat pada induk bunting terhadap ekspresi gen insulin pada dentate gyrus. Penelitian dilakukan di UPHL IPB pada bulan Mei 2015 hingga Desember 2016 dengan  84 ekor anak tikus yang dilahirkan oleh induk tikus kontrol yang diberi asam valproat 250 mg pada umur kebuntingan 10, 13, dan 16 hari digunakan untuk pengamatan kadar glukosa, insulin, DNA, RNA, dan rasio RNA/DNA serta pengamatan mikroskopis otak. Pengamatan dilakukan selang waktu empat minggu, dimulai dari umur 4 sampai 32 minggu. Anak tikus yang dilahirkan oleh induk tikus yang diberi asam valproat selama kebuntingan mempunyai kadar glukosa otak yang lebih tinggi (p<0,01) dan insulin yang lebih rendah (p<0,05).  Selama periode pertumbuhan, anak tikus yang dilahirkan oleh induk tikus yang diberi asam valproat mengalami peningkatan kadar glukosa dan penurunan kadar insulin (p<0.05). Pengamatan mikroskopis sel-sel dentate gyrus menunjukkan degenerasi sel dan tidak terlihat reaksi imunoreaktif terhadap insulin, namun terjadi penurunan konsentrasi DNA, RNA, serta rasio RNA/DNA (p<0,05). Pemberian asam valproat pada induk tikus pada umur kebuntingan 10, 13, dan 16 hari memengaruhi organogenesis otak anak tikus sehingga menyebabkan kerusakan sel-sel saraf penghasil insulin otak yang ditunjukkan oleh  penurunan sekresi dan kadar insulin. [MKB. 2017;49(3):156–64]Kata kunci: Asam valproat, dentate gyrus, insulin, organogenesis Valproic Acid Administration in Pregnant Rats Inhibits Insulin Synthesis n in Brain Cells of the OffspringsValproic acid affects the activity of histone deacetylase which eventually affects gene expression during organogenesis. Insulin plays a role in regulating the proliferation and differentiation of the dentate gyrus. This experiment was designed to study the effects of valproic acid administration on the expression of insulin gene in the dentate gyrus of pregnant rats.  This study was carried out at the Laboratory Animal Management Unit (UPHL) of Bogor Agricultural University  from May 2015 to December 2016, with eighty four newborn rats from control rats and VA-administered rats at the age of 10, 13, and 16 days of pregnancy. The measurements were done for glucose, insulin, DNA, and RNA concentrations in dentate gyrus and to observe the microscopic condition of the brain at the ages of 4 to 32 weeks with 4 weeks interval.  Rats born form mothers administered by 250 mg valproic acid during pregnancy had higher glucose concentrations (p<0.01) and lower insulin concentrations (p<0.05) in the dentate gyrus. During the post-natal growth phase, rats born from mothers administered with valproic acid had increased glucose concentrations and decreased insulin concentrations (p<0.05). Microscopic observations of the dentate gyrus showed degeneration of nerve cells with no immunoreaction to insulin was seen in dentate gyrus cells. However, the concentrations of DNA, RNA, and the ratio of RNA/DNA in the dentate gyrus cells decreased (p<0.05). The administration of valproic acid to pregnant rats at the age of 10, 13, and 16 days of pregnancy destructs  brain organogenesis of the offsprings which eventually disturbs insulin production in the dentate gyrus indicated by decreased insulin secretion and concentrations.  [MKB. 2017;49(3):156–64]Key words: Dentate gyrus, insulin, organogenesis, valproic acid
DAMPAK ERUPSI GUNUNG MERAPI TERHADAP LAHAN DAN UPAYA-UPAYA PEMULIHANNYA Rahayu, Rahayu; Ariyanto, Dwi Priyo; Komariah, Komariah; Hartati, Sri; Syamsiyah, Jauhari; Dewi, Widyatmani Sih
Caraka Tani - Jurnal Ilmu Ilmu Pertanian Vol 29, No 1 (2014): Articles in Press
Publisher : Caraka Tani - Jurnal Ilmu Ilmu Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

UPAYA HUKUM PERMOHONAN EKSEKUSI TERHADAP PUTUSAN NAFKAH HADHANAH (Studi Pelaksanaan Putusan Terhadap Putusan Pengadilan Agama Nomor : 0957/Pdt.G/2014/PA.MLG) Cahyani, Tinuk Dwi Cahyani; Komariah, Komariah
Legality : Jurnal Ilmiah Hukum Vol 25, No 1 (2017)
Publisher : Faculty of Law, University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (460.238 KB) | DOI: 10.22219/jihl.v25i1.5994

Abstract

The judge's ruling on the provision of a living has to be protected from legal certainty and justice in the decision Number: 0957 / Pdt.G / 2014 / PA.Mlg if the Petitioner (Defendant) is a civil servant (PNS). Implementation of the provision of income by the Petitioner (Defendant Reconciliation) to the Respondent (Plaintiff of Reconstruction) in the decision Number: 0957 / Pdt.G / 2014 / PA.Mlg. The supporting or inhibiting factor in the provision of the provision of income by the Petitioner (Defendant) to the Respondent (Plaintiff) in Decision Number: 0957 / Pdt.G / 2014 / PA.Mlg which has permanent legal force. To know the implementation of the decision, it is necessary to do research which is a sociological juridical research, using Primary data type which is a decision Number: 0957 / Pdt.G / 2014 / PA.Mlg. Data collection using the analysis of the decision that has been legally fixed (inkracht), interviews, documentation and literature study. The study was taken from the decision of the Panel of Judges in the Religious Courts of Malang and the interviews of the Respondent / Plaintiffs of Reconstruction. in order to be a reference for readers. Meanwhile, there are still obstacles in Malang education office that can not be interviewed because it is not willing to be questioned or information on the grounds there has never been a similar case. The result of research has been done by the researcher that the Petitioner / Defendant of Reconstruction does not fulfill its obligation as it existed in the ruling Decision Number: 0957 / Pdt.G / 2014 / PA.Mlg which is the basis of judge consideration already has elements of justice for the parties because it is in accordance with the circumstances and the ability of the parties to perform their respective obligations. Applicant/Defendant Rekonpensi able to carry out its obligations as an educator as well as a Civil Servant (PNS). Since the verdict was read by the Panel of Judges who had been inkracht, the Petitioner / Defendant of the Reconstruction for three years ignored or neglected his obligations (hadhanah's living). No seizure of guarantee or sanction if Petitioner / Defendant Reconcile neglects to its obligation.
PELINDIAN UNSUR KALIUM (K) DAN NATRIUM (Na) MATERIAL VULKANIK HASIL ERUPSI GUNUNG MERAPI 2010 (Simulasi Laboratorium) Sanjaya, Taufiq Perak; Syamsiyah, Jauhari; Ariyanto, Dwi Priyo; Komariah, Komariah
Caraka Tani - Jurnal Ilmu Ilmu Pertanian Vol 29, No 2 (2014)
Publisher : Caraka Tani - Jurnal Ilmu Ilmu Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This research was conducted at the Greenhouse Laboratory of the Faculty of Agriculture, Sebelas Maret University Surakarta, in March 2011. The aim of the study was to determine the relationship between the rainfall (synthetic) the number of elements Potassium (K) and Sodium (Na) were leached in volcanic material from the eruption of Mount Merapi. This study used artificial rainfall simulation approach  to treatment is based on the phenomenon of the eruption of Mount Merapi, Yogyakarta, Central Java by examining the leaching elements Sodium (Na) and Potassium (K) are swapped in the above-ground volcanic material Andisol. The experiments in this study using a completely randomized design (CRD) consisting of 3 to 5 treatments the sample depth artificial rain (water sprinkling) in 3 replications, namely 1 (22 mm x 1 day (22 mm)), 2 (22 mm x 10 days (220 mm)), 3 (22 mm x 20 days (440 mm)), 4 (22 mm x 30 days (660 mm)), 5 (22 mm x 45 days (990 mm)), and control ( 45 days field) with 3 replicates each. The variables measured were pH H2O, available K and Na, K and Na total, CEC, texture. Analysis of the data using the F test at the level of 95% or Kruskal-Wallis, to compare the mean between treatments using DMR test at the level of 95% or Mood Median, whereas to determine the relationship between variables used correlation test. The results showed that administration of rainfall of 22 mm / day is able to affect the leaching of potassium and sodium in the volcanic material from day 10. Leaching was significantly visible on day 30 with a decrease in available K and Na volcanic material at a depth of 0-15 cm in diameter (K 23.08%) (23.08% Na) and at a depth of 15-30 cm (K 21, 42%) (23.08% Na), and the addition of K and Na are available at a depth of &gt;30 cm in the form of land Andisol (K 16.67%) (23.53% Na) from the previous treatment.
DAMPAK ERUPSI GUNUNG MERAPI TERHADAP LAHAN DAN UPAYA-UPAYA PEMULIHANNYA Rahayu, Rahayu; Ariyanto, Dwi Priyo; Komariah, Komariah; Hartati, Sri; Syamsiyah, Jauhari; Dewi, Widyatmani Sih
Caraka Tani: Journal of Sustainable Agriculture Vol 29, No 1 (2014): March
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v29i1.13320

Abstract

The eruption of Merapi mountain has primary and secondary hazard and may damage to the land. In detail, the hazards are land degradation is a loss of some or many of germplasm and changes in plant biodiversity. The others hazard including loss of water catchment areas, the destruction of forests, and even the closing of the water source, as well as the loss of water channels. The burried of soil and soil formation inhibition were caused by the repeated eruptions of Merapi, beside the loss of roads access to agricultural land and loss of land ownerships boundaries by the eruption and cool lava. Materials of eruption are sand and pyroclastic materials, as well as the nature of cementation require special techniques and technology to use the land as new farmland. Land restoration efforts can be done with the land management by reforestation on government-owned land for water catchment function, agroforestry forage grass based, grazing field on land owned by the village and residents, with the use of organic materials in the eruption sandy soil ameliorant.
KEKONVERGENAN BARISAN DI DALAM RUANG TOPOLOGI YANG DIBANGUN OLEH METRIK-θ Komariah, Komariah
Jurnal Mahasiswa Matematika Vol 3, No 5 (2015)
Publisher : Jurnal Mahasiswa Matematika

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

PENERAPAN MODEL PEMBELAJARAN COOPERATIVE-MEANINGFUL INSTRUCTIONAL DESIGN (C-MID) TERHADAP PENINGKATAN KEMAMPUAN KOMUNIKASI MATEMATIS SISWA komariah, Komariah; Rosyid, Abdul; Nuraeni, Zuli
JUMLAHKU: Jurnal Matematika Ilmiah STKIP Muhammadiyah Kuningan Vol 3 No 2 (2017): Edisi Vol 3 No 2 November
Publisher : Program Studi Pendidikan Matematika STKIP Muhammadiyah Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1633.433 KB)

Abstract

Application of Cooperative-Meaningful InstructionalDesign (C-MID) Learning Model to Improve Student Mathematical CommunicationSkill. This study aims to examine the improvement of students mathematicalcommunication skills with Cooperative-Meaningful Instructional Design learningmodel. This research involves experimental class and control class which is takenfrom class VII (Seven) SMP Negeri 4 Kuningan academic year 2016/2017. Theinstruments used in this study are test and questionnaire. Through experimentalmethod of pretest result it is known that the average score of mathematicalcommunication ability of control class is 31,36 while experiment class is 27,15. Andresult of posttest known that average score of communication of control class is 37,17and mean score of mathematical communication of experiment class is 43,08. Fromthe calculation of N-Gain score, the average of experimental class is 0,45 and thecontrol class is 0,26, it shows that after being given treatment of improvement ofmathematical ability of experiment class communication better than improvement ofmathematical communication ability of control class. The improvement ofmathematical communication skills of the experimental class is in the mediumcategory. In addition, from the result of questionnaire of student response, it is knownthat the students showed a positive response to the C- Cooperative-MeaningfulInstructional Design (C-MID) learning model.