Articles

Found 10 Documents
Search

SUPEROKSIDA DISMUT ASE (SOD) : APA DAN BAGAIMANA PERANANNYA DALAM RADIOTERAPI Nurhayati, Siti; Kisnanto, Teja; Syaifudin, Mukh
Buletin Alara Vol 13, No 2: Desember 2011
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tidak ada abstrak
RESPONS HEMATOPOITIK MENCIT YANG DIINFEKSI DENGAN Plasmodium berghei STADIUM ERITROSITIK IRADIASI GAMMA Darlina, .; Kisnanto, Teja; Fauzan, Ahmad
Jurnal Sains dan Teknologi Nuklir Indonesia Vol 13, No 2 (2012): Agustus 2012
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.897 KB)

Abstract

RESPONS HEMATOPOITIK MENCIT YANG DIINFEKSI DENGAN Plasmodiumberghei STADIUM ERITROSITIK IRADIASI GAMMA. Salah satu strategi untuk mengontrolpenyakit malaria adalah pengembangan vaksin melalui pelemahan parasit Plasmodium bergheidengan iradiasi gamma. Pada penelitian ini, P. berghei yang diiradiasi gamma dan yang tidakdiiradiasi disuntikkan secara peritoneal pada mencit untuk mempelajari respons hematopoitik.Respons hematopoitik ditentukan melalui persen parasitemia, jumlah eritrosit, leukosit, limfosit,dan monosit setiap 2 hari sekali selama 14 hari. Berat organ limpa dan hati mencit diukursetiap 3 hari sesudah infeksi. Mencit yang diinfeksi dengan parasit yang diiradiasi memilikiperiode prepaten 5 hari dengan parasitemia rendah dan jumlah eritrosit mengalami sedikitpenurunan. Jumlah leukosit naik hampir dua kali dari konsentrasi awal, dan jumlah limfosit sertamonosit juga mengalami kenaikan. Mencit yang diinfeksi dengan P. berghei tanpa iradiasimemiliki periode prepaten 2 hari dengan parasitemia meningkat, jumlah eritrosit mengalamipenurunan hingga 75%, dan jumlah leukosit tidak mengalami peningkatan. Limpa maupun hatimencit yang diinfeksi dengan P. berghei yang diiradiasi mengalami sedikit kenaikan berat,sedangkan pada mencit yang diinfeksi dengan P. berghei tanpa iradiasi terjadi kenaikan yangsignifikan. Peningkatan jumlah leukosit, limfosit, monosit, dan rendahnya parasitemia padamencit yang diinfeksi P. berghei yang diiradiasi menunjukkan terjadinya respons imun padamencit.
Correlation Between Akt and p53 Protein Expression and Chemoradiotherapy Response in Cervical Cancer Patients KURNIA, IIN; SIREGAR, BUDININGSIH; SOETOPO, SETIAWAN; RAMLI, IRWAN; KURJANA, TJAHYA; ANDRIONO, .; TOBING, MARINGAN DIAPARI LUMBAN; SURYAWATHI, BETHY; KISNANTO, TEJA; TETRIANA, DEVITA
HAYATI Journal of Biosciences Vol 21, No 4 (2014): Desember 2014
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1416.842 KB) | DOI: 10.4308/hjb.21.4.173-179

Abstract

Akt is a protein that is associated with cell proliferation and is expressed at high levels in cancer cells. Some research indicates it may play a role in increasing the resistance of cancer cells to chemotherapy treatment. P53 is a tumor suppressor protein that influences the cell cycle and apoptosis. The purpose of this study was to examine the relationship between the expression of Akt and p53 in cancerous tissue before chemoradiation treatment, and the clinical response to treatment of cervical cancer patients. Twenty microscopic tissue samples were taken from cervical cancer biopsies obtained from patients before cancer treatment. The tissue samples were stained with p53 and Akt antibodies via immunohistochemistry technique, to measure expression of both proteins. After completion of chemoradiotherapy, patients’ clinical response to treatment was determined using the pelvic control method. Our results revealed no correlation between expression of Akt and p53 index (P = 0.74) as well as between p53 Index and chemoradiotherapy clinical response (P=0.29). There was significant correlation between expression of Akt and cervical cancer chemoradiotherapy response (P = 0.03). There was no correlation found between p53 index and chemoradiotherapy clinical response (P = 0.29). High expression of Akt may related with high cell proliferation and resistance to chemoradiotherapy.
Pendeteksian Ekspresi Biomarker ERK Secara Semi Kuantitatif dan Kuantitatif Pada Kanker Serviks Sebelum Respon Kemoradioterapi Kisnanto, Teja; Wardani, Rina Tri; Siregar, Budiningsih; Amir, Mellova; Soetopo, Setiawan; Ramli, Irwan; Kurjana, Tjahya; Andrijono, Andrijono; S Hernowo, Bethy; DL Tobing, Maringan; Tetriana, Devita
Jurnal Keselamatan Radiasi dan Lingkungan Vol 1, No 1 (2016): Juni 2016
Publisher : Pusat Teknologi Keselamatan dan Metrologi Radisasi - BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (371.099 KB)

Abstract

Kanker servik merupakan penyakit kanker yang umum dijumpai pada wanita yang disebabkan oleh virus HPV (Human Papilova Virus). Tujuan penelitian ini adalah untuk mengetahui hubungan ekspresi protein MNK (Mitogen Activated Protein Kinase) pada penderita kanker serviks sebelum tindakan pengobatan terhadap respon kemoradioterapi. Sampel uji yang digunakan adalah sediaan mikroskopis hasil biopsi jaringan kanker dari penderita kanker serviks stadium lanjut (IIB-IIIB) sebanyak 20 sampel. Metode yang digunakan adalah metode imunohistokimia dengan menggunakan biomarker MNK pada biopsi jaringan kanker serviks. Ekspresi protein MNK yang positif ditandai dengan warna coklat tua yang terdapat pada inti sel. Respon kemoradioterapi diperoleh dari RSUPN Dr. Cipto Mangunkusumo Jakarta dan RS Hasan Sadikin Bandung. Hasil penelitian menunjukkan nilai IRS (Imuno Reaktif Score) protein MNK pada grup respon kemoradioterapi baik lebih tinggi dibandingkan grup respon kemoradioterapi buruk dan tidak ditemukan adanya hubungan IRS protein MNK dengan respon kemoradioterapi. Sedangkan hubungan ekspresi MNK terhadap respon kemoradioterapi menunjukkan adanya korelasi perbedaan grup respon kemoradioterapi antara ekspresi protein MNK negatif dan ekspresi protein MNK positifCervical cancer is a cancer that common in women caused by HPV (Human Papilova Virus). The purpose of this study is to determine the relationship MNK protein expression (Mitogen-Activated Protein Kinase) in patients with cervical cancer before chemoradiotherapy treatment. Sample used was the preparation of microscopic cancer tissue biopsies from patients with advanced cervical cancer (IIB-IIIB) is 20 samples. The method used is immunohistochemistry using MNK biomarkers in cervical cancer tissue biopsies. MNK positive protein expression marked with dark brown color that is contained in the cell nucleus. Chemoradiotherapy response obtained from RSUPN Dr. Cipto Mangunkusumo and Hasan Sadikin Hospital in Bandung. The results show the value of the IRS (Immuno Reactive Score) MNK protein in response to chemoradiotherapy group either higher than the response to chemoradiotherapy group was bad and did not find any relationship IRS MNK protein with chemoradiotherapy response. While the relationship MNK expression responses show a correlation chemoradiotherapy group differences in chemoradiotherapy response between MNK expression negative and MNK expression positive.
Pendeteksian Ekspresi Biomarker MNK Secara Semi Kuantitatif dan Kuantitatif Pada Kanker Serviks Sebelum Respon Kemoradioterapi Kisnanto, Teja; Wardani, Rina Tri; Siregar, Budiningsih; Amir, Mellova; Soetopo, Setiawan; Ramli, Irwan; Kurjana, Tjahya; Andrijono, A; Hernowo, Bethy S; Tobing, Maringan DL; Tetriana, Devita
Jurnal Keselamatan Radiasi dan Lingkungan Vol 1, No 2 (2016): Desember 2016
Publisher : Pusat Teknologi Keselamatan dan Metrologi Radisasi - BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.883 KB)

Abstract

Kanker servik merupakan penyakit kanker yang umum dijumpai pada wanita yang disebabkan oleh virus HPV (Human Papilova Virus). Tujuan penelitian ini adalah untuk mengetahui hubungan ekspresi protein MNK (Mitogen Activated Protein Kinase) pada penderita kanker serviks sebelum tindakan pengobatan terhadap respon kemoradioterapi. Sampel uji yang digunakan adalah sediaan mikroskopis hasil biopsi jaringan kanker dari penderita kanker serviks stadium lanjut (IIB-IIIB) sebanyak 20 sampel. Metode yang digunakan adalah metode imunohistokimia dengan menggunakan biomarker MNK pada biopsi jaringan kanker serviks. Ekspresi protein MNK yang positif ditandai dengan warna coklat tua yang terdapat pada inti sel. Respon kemoradioterapi diperoleh dari RSUPN Dr. Cipto Mangunkusumo Jakarta dan RS Hasan Sadikin Bandung. Hasil penelitian menunjukkan nilai IRS (Imuno Reaktif Score) protein MNK pada grup respon kemoradioterapi baik lebih tinggi dibandingkan grup respon kemoradioterapi buruk dan tidak ditemukan adanya hubungan IRS protein MNK dengan respon kemoradioterapi. Sedangkan hubungan ekspresi MNK terhadap respon kemoradioterapi menunjukkan adanya korelasi perbedaan grup respon kemoradioterapi antara ekspresi protein MNK negatif dan ekspresi protein MNK positif. Cervical cancer is a cancer that common in women caused by HPV (Human Papilova Virus). The purpose of this study is to determine the relationship MNK protein expression (Mitogen-Activated Protein Kinase) in patients with cervical cancer before chemoradiotherapy treatment. Sample used was the preparation of microscopic cancer tissue biopsies from patients with advanced cervical cancer (IIB-IIIB) is 20 samples. The method used is immunohistochemistry using MNK biomarkers in cervical cancer tissue biopsies. MNK positive protein expression marked with dark brown color that is contained in the cell nucleus. Chemoradiotherapy response obtained from RSUPN Dr. Cipto Mangunkusumo and Hasan Sadikin Hospital in Bandung. The results show the value of the IRS (Immuno Reactive Score) MNK protein in response to chemoradiotherapy group either higher than the response to chemoradiotherapy group was bad and did not find any relationship IRS MNK protein with chemoradiotherapy response. While the relationship MNK expression responses show a correlation chemoradiotherapy group differences in chemoradiotherapy response between MNK expression negative and MNK expression positive.
Correlation Between Akt and p53 Protein Expression and Chemoradiotherapy Response in Cervical Cancer Patients KURNIA, IIN; SIREGAR, BUDININGSIH; SOETOPO, SETIAWAN; RAMLI, IRWAN; KURJANA, TJAHYA; ANDRIONO, .; TOBING, MARINGAN DIAPARI LUMBAN; SURYAWATHI, BETHY; KISNANTO, TEJA; TETRIANA, DEVITA
HAYATI Journal of Biosciences Vol 21, No 4 (2014): December 2014
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1416.842 KB) | DOI: 10.4308/hjb.21.4.173

Abstract

Akt is a protein that is associated with cell proliferation and is expressed at high levels in cancer cells. Some research indicates it may play a role in increasing the resistance of cancer cells to chemotherapy treatment. P53 is a tumor suppressor protein that influences the cell cycle and apoptosis. The purpose of this study was to examine the relationship between the expression of Akt and p53 in cancerous tissue before chemoradiation treatment, and the clinical response to treatment of cervical cancer patients. Twenty microscopic tissue samples were taken from cervical cancer biopsies obtained from patients before cancer treatment. The tissue samples were stained with p53 and Akt antibodies via immunohistochemistry technique, to measure expression of both proteins. After completion of chemoradiotherapy, patients? clinical response to treatment was determined using the pelvic control method. Our results revealed no correlation between expression of Akt and p53 index (P = 0.74) as well as between p53 Index and chemoradiotherapy clinical response (P=0.29). There was significant correlation between expression of Akt and cervical cancer chemoradiotherapy response (P = 0.03). There was no correlation found between p53 index and chemoradiotherapy clinical response (P = 0.29). High expression of Akt may related with high cell proliferation and resistance to chemoradiotherapy.
Capability of Vitamin E as a Radioprotector in Suppressing DNA Damage Determined with Comet Assay Darlina, Darlina; A., Lusy Dahlia; Alatas, Zubaidah; Kisnanto, Teja; Syaifudin, Mukh
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8333

Abstract

Radiation has a potent to damage cells. Radiation may act directly or indirectly on deoxyribonucleic acid (DNA) that results in the degeneration of tissues and necrotic, and thereby it needs a potent radioprotector to prevent these damages. Vitamin E is natural product known as an antioxidant which has potential as radioprotector. This research aimed to determine the capability of vitamin E with emphasized on the searching for its optimal concentration as radioprotector of DNA damage. This study used blood samples of healthy person irradiated with gamma rays at a dose of 6 Gy as the lethal dose to lymphocytes. The cocentrations of vitamin E from 0 to 0.8 mM was added into blood 15 minutes before irradiation. Isolation of lymphocytes was done using gradient centrifugation method. Evaluation on the capability of this compound in suppressing DNA damage was done by using alkaline Comet assay and data analysis was done using CaspLab program. The results show that addition of vitamin E could suppres these DNA damages and 0.8 mM of vitamin could reduce DNA damage up to 94.2%. We conclude that vitamin E effectively suppresed DNA damages induced by radiation. This information may benefit to the patient from negative impacts of radiotherapy.
Pengaruh Radiasi Pengion Terhadap Kerusakan DNA pada Sel Limfosit Pekerja Medis dengan Menggunakan Uji Komet Kisnanto, Teja; Darlina, Dalina; Raharjo, Tur
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 14, No 2 (2018): Desember 2018
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.427 KB) | DOI: 10.17146/jair.2018.14.2.4664

Abstract

              Pemanfaatan teknologi nuklir dibidang medis sudah banyak digunakan untuk diagnosa maupun terapi. Pasien maupun pekerja radiasi yang bertindak sebagai operator yang membantu pasien berisiko terpapar radiasi. Paparan radiasi pengion dapat menginduksi mutasi dan transformasi sel terutama sebagai konsekuensi dari kerusakan pada DNA Dengan demikian perlu dievaluasi kerusakan DNA dengan membandingkan dengan kontrol. Uji Comet merupakan salah satu biomarker untuk mengevaluasi kerusakan DNA karena paparan radiasi dengan mengukur tingkat migrasi DNA di limfosit darah tepi. Salah satu parameter yang umum digunakan pada uji komet adalah panjang ekor komet (Tail Length/TL). Pada penelitian ini dilakukan analisis TL pada citra komet dari sampel pekerja radiasi medis suatu Rumah Sakit di Jakarta. Hasil penelitian menunjukan bahwa TL kelompok pekerja radiasi medis lebih tinggi secara bermakna dibandingkan kelompok kontrol. Nilai TL pada pekerja radiasi medis pria lebih tinggi dibandingkan wanita. Dengan demikian aspek proteksi dan keselamatan radiasi terhadap paparan radiasi dosis rendah tidak dapat diabaikan lagi dan harus mulai untuk diprioritaskan.
Irradiation of intraerythrocytic Plasmodium berghei with a fractionated dose of gamma rays does not effectively reduce the infectivity in mice Mus musculus Syaifudin, Mukh; Nurhayati, Siti; Darlina, Darlina; Lusiyanti, Yanti; Kisnanto, Teja
Aceh Journal of Animal Science Vol 4, No 1: July 2019
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1783.525 KB) | DOI: 10.13170/ajas.4.1.13558

Abstract

Malaria infection kills more than one million human every year, mainly under-5-year-old children, including in South East Asian nations. Gamma radiation given at a single dose is commonly used to create the attenuated Plasmodium parasites to get vaccine materials. However, there is no study on the infectivity of parasites after fractionated γ-radiation. This study aimed to assess the infectivity of parasites after irradiated with fractionated γ-rays in mice. A number of Plasmodium bergheithat was irradiated in two fractions of 100 and 50 Gy, 100 and 75 Gy; and 100 and 100 Gy within 5 minutes of interval time was injected intraperitoneally into 12 mice. Mice injected with unirradiated parasites (0 Gy) served as a control group. The parasitemia level of intraerythrocytic parasites in each group was observed at days post injection up to 20 days by making Giemsa stained thin blood smears and observed under the microscope. Results showed that fractionation radiation did not effectively attenuate the parasites where they still grew in blood of mice, except for 100+75 Gy. There are no significant differences among the treatment groups (p>0.05). This is different from irradiation at the single dose that resulted in almost completely attenuated parasites mainly the dose of 150 Gy. This implicating that irradiation of gamma rays at a single dose is a better way to mitigate parasites than fractionation dose as the infectivity of irradiated parasites were lower compared to that of fractionated dosage. Keywords: Malaria vaccine, Gamma radiation, Fractionation, Parasitemia
Efek Vaksinasi Berulang Plasmodium berghei Radiasi dalam Menginduksi Kekebalan Humoral dan Proteksi pada Mencit (Mus musculus) Swiss Webster Yusuf, Darlina; Rahardjo, Tur; Nurhayati, Siti; Kisnanto, Teja
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 15, No 1 (2019): JUNI 2019
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.104 KB) | DOI: 10.17146/jair.2019.15.1.4659

Abstract

Radiasi gamma dapat digunakan untuk melemahkan parasit. Parasit yang lemah memberikan kesempatan bagi inangnya untuk mengembangkan respon imun yang mampu mengatasi penyakit paska infeksi. Antibodi merupakan komponen kekebalan tubuh yang berperan dalam respon imun humoral. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh imunisasi berulang dan uji tantang terhadap respon antibodi dan pertumbuhan parasit. Penelitian ini menggunakan rancang acak dengan 60 ekor mencit Swiss Webster yang dibagi menjadi 3 kelompok dan dilakukan selama 62 hari. Dosis radiasi yang digunakan adalah 150 Gy, 175 Gy, dan kontrol positif (0 Gy), dosis imunisasi dengan 1 x 107 parasit diradiasi per ml. Imunisasi ulang dilakukan 1 dan 2 minggu setelah imunisasi pertama.  Uji tantang dilakukan satu bulan setelah imunisasi pertama. Pertumbuhan parasit diamati setiap dua hari dan pengamatan respon antibodi diamati pada hari ke - 8, 15, 40, 47, dan 62. Hasil yang diperoleh adalah, konsentrasi antibodi mencit diimunisasi dengan dosis 175 Gy meningkat hingga 3 kali setelah imunisasi kedua, pertumbuhan parasit relatif rendah, dan 60% mencit tetap sehat hingga hari ke 62. Dapat disimpulkan bahwa imunisasi ulangan P. berghei yang diradiasi 175 Gy dapat meningkatkan konsentrasi antibodi, menekan pertumbuhan parasit, mencegah manifestasi gejala klinis dan mencit tetap sehat sampai hari ke – 62.