Bambang Kiranadi
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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Injeksi Clenbuterol Menurunkan Lemak Bawah Kulit dan Meningkatkan Bobot Karkas Kambing Pernakan Etawah

Jurnal Veteriner Vol 10, No 2 (2009)
Publisher : Jurnal Veteriner

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Abstract

Twenty growing cross breed etawah goat were treated with clenbuterol every two days. Three levels ofclenbuterol , 5, 10 and 20 ?g/kg BW were injected intramuscularly from the thigh side. The experimentwould like to see the effect of clenbuterol on the metabolism and carcase quality. It was found thatclenbuterol did not affect the gross energy, digestibility, metaboliseable energy and urinary nitrogenexcretion.Clenbuterol will increase the feed intake up to 51.49%. Althoush not affecting protein intake, itsignificantly increased the retain protein from 70.51 up to 149.37%. Subcutaneous carcase weights werereduced between 29.6 up to 51%. Data from carcase quality showed that clenbuterol increase half emptybody weight indicating that clenbuterol is affecting protein metabolism. Dose -respond curve of clenbuterolagainst half empty body weight follows the Michaelis Menten equation and solved by Lineweaver-Burks.Result indicated that the half weight maximum was 5120 gram and Km of clemnbuterol was 9.50 x 10-8M. Clenbuterol is affecting the bone weight carcase but not affecting the intermusculer carcase.

Distribusi Otot, Lemak dan Tulang pada Karkas Kambing Jantan Peranakan Etawah yang Diberi Suntikan Clenbuterol (DISTRIBUTION OF MUSCLE, FAT AND BONE IN MALE ETAWAH CROSS BREED BUCKS TREATED WITH CLENBUTEROL)

Jurnal Veteriner Vol 13, No 4 (2012)
Publisher : Jurnal Veteriner

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Abstract

Twenty growing cross breed etawah buck had been treated with clenbuterol every two days. Threelevels of clenbuterol, 5, 10 and 20 ìg/kg BW were injected intramuscularly from the thigh side every twodays. The animals were slaughtered and cut into five pieces, thorax, fore limb, loin, flank and hind limb.The components of muscle and fat for each component were separated for fat, muscle and bones analysis.The purpose of the experiment was to investigate the claim that clenbuterol has an effect on carcassquality. It is expected that clenbuterol will affect fat mobilization through the mechanism of stimulusrespond . In this experiment clenbuterol does not affect fresh carcass weight and carcasss empty bodyweight. However clenbuterol has the effect on muscle carcass thigh, thorax and flank, indicating proteinformation in the carcass. Further studies showed that clenbuterol affected the reduction of subcutanousfat in thigh and flank. Intermuscular fat of the thorax was also affected by clenbuterol. Furthermoreclenbuterol reduced the bones weight. It can be concluded that clenbuterol works at specific area of thebody, mobilizing the fat and bones follow with protein synthesis.

Pemberian Ekstrak Etanol Purwoceng pada Masa Pascaplasentasi Meningkatkan Kinerja Reproduksi Tikus Bunting (SUPPLEMENTATION ETHANOLIC EXTRACT OF PIMPINELLA ALPINA AT POSTPLASENTATION ON PREGNANT RAT IMPROVE REPRODUCTIVE PERFORMANCE)

Jurnal Veteriner Vol 17, No 1 (2016)
Publisher : Jurnal Veteriner

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Abstract

The study was carried out to observe the effect of ethanolic extract of estrogenic purwoceng given 13-21 days age of pregnancy on reproductive performance such as the weight of ovarium, uterus, placenta, andpups, correlation ratio of corpus luteum number, implantation site number, and litter size in rats. Thepregnant rats were divided in to two groups. One groups cosisted of rats treated with purwoceng (83.33 mg/kg body weight) and the other groups was used as control (no treatment). The ovary, uterus, placenta, andpups were collected and weighed on day 21 of pregnancy. The result showed that the rats given 83,33 mg/kg BW ethanol extract of purwoceng tended to increase the weight of ovarium, uterus, and pups but it didnot increase the weight of plasenta. Number of site of implantation, ratio of implantation site to numberof corpus luteum, and ratio of number of pups to implantation site were higher on rat given 83,33 mg/kgBW ethanol extract of purwoceng. Conclusion of this study is ethanol extract of Pimpinella alpina rootsgiven at postplasentation improve reproductive performance.

PENGARUH BEBAN KERJA OSMOTIK TERHADAP PERKEMBANGAN DAN KELANGSUNGAN HIDUP LARVA UDANG GALAH Macrobrachium rosenbergii DE MAN

Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol 13, No 1 (2006): Juni 2006
Publisher : Institut Pertanian Bogor

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Abstract

Rendahnya derajat kelangsungan hidup adalah salah satu kendala pada pembenihan udang galah.Kendala tersebut dapat diatasi dengan pengaturan salinitas. Penelitian ini bertujuan untuk menentukan salinitas optimum untuk perkembangan larva udang galah dengan tingkat kematian yang rendah yang ditetaskan pada salinitas 6 ppt. Hari pertama hingga hari ketujuh setelah menetas, salinitas ditingkatkan secara bertahaphingga mencapai 10.2 ppt, 11.6 ppt, 13.0 ppt, dan 14.4 ppt dan selanjutnya salinitas dipertahankan tetap hingga seluruh larva tumbuh menjadi post larva. Hasil penelitian menunjukkan bahwa salinitas 13 ppt menghasilkan beban osmotic yang terendah, mempercepat waktu perkembangan larva dan derajat kelangsungan hidupyang tertinggi.Kata kunci: larva udang galah, pengaturan osmotik, pertumbuhan, kelangsungan hidup, tingkat kematian.

Trehalose preincubation increases mesenchymal (CD271+) stem cells post-cryopreservation viability

Medical Journal of Indonesia Vol 25, No 3 (2016): September
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Background: Dimethyl sulfoxide (Me2SO) is a common cryoprotective agent widely used in cell preservation system. Me2SO is currently known to cause epigenetic changes which are  critical in stem cells development and cellular differentiation. Therefore, it is imperative to develop cryopreservation techniques that protect cellular functions and avert Me2SO adverse effect. Trehalose was able to protect organism in extreme condition such as dehydration and cold. This study aimed to verify the protective effect of trehalose preincubation procedure in cryopreservation.Methods: The study was conducted using experimental design. Thawed mesenchymal (CD271+) stem cells from YARSI biorepository were used for the experiment. Trehalose preincubation was performed for 1 hour, internalized trehalose was confirmed by FTIR-ATR measurement. Three groups consisted of (1) cryopreserved without trehalose preincubation, (2) cryopreserved with trehalose preincubation, and (3) did not undergo cryopreservation were evaluated after 24 hours in LN2 for viability in culture. The absorbance from each group was measured at 450 nm. The analysis performed using paired student t test.Results: Viability of thawed mesenchymal (CD271+) stem cells that undergo trehalose preincubation prior cryopreservation was significantly higher (p<0.05) compared to group without trehalose preincubation. Higher viability observed between group with trehalose preincubation compared with controlled group suggests protection to trypsinization. Mesenchymal (CD271+) stem cells incubated for 1 hour in 100 mM trehalose supplemented medium  results in 15%  trehalose loading efficiency.Conclusion: These findings confirm the protective effect of trehalose preincubation in cryopreservation. Future research should be directed to elucidate the trehalose internalization mechanism and eventually the protective mechanism of trehalose in mammalian cell cryopreservation.

Pemberian Asam Valproat pada Induk Tikus Bunting Menghambat Sintesis Insulin pada Sel Otak Anak Tikus

Majalah Kedokteran Bandung Vol 49, No 3 (2017)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Asam valproat memengaruhi aktivitas histone deacetylase yang berperan dalam ekspresi gen selama organogenesis. Insulin berperan dalam proliferasi dan diferensiasi sel-sel saraf dentate gyrus hipokampus. Penelitian ini bertujuan mempelajari pengaruh pemaparan asam valproat pada induk bunting terhadap ekspresi gen insulin pada dentate gyrus. Penelitian dilakukan di UPHL IPB pada bulan Mei 2015 hingga Desember 2016 dengan  84 ekor anak tikus yang dilahirkan oleh induk tikus kontrol yang diberi asam valproat 250 mg pada umur kebuntingan 10, 13, dan 16 hari digunakan untuk pengamatan kadar glukosa, insulin, DNA, RNA, dan rasio RNA/DNA serta pengamatan mikroskopis otak. Pengamatan dilakukan selang waktu empat minggu, dimulai dari umur 4 sampai 32 minggu. Anak tikus yang dilahirkan oleh induk tikus yang diberi asam valproat selama kebuntingan mempunyai kadar glukosa otak yang lebih tinggi (p<0,01) dan insulin yang lebih rendah (p<0,05).  Selama periode pertumbuhan, anak tikus yang dilahirkan oleh induk tikus yang diberi asam valproat mengalami peningkatan kadar glukosa dan penurunan kadar insulin (p<0.05). Pengamatan mikroskopis sel-sel dentate gyrus menunjukkan degenerasi sel dan tidak terlihat reaksi imunoreaktif terhadap insulin, namun terjadi penurunan konsentrasi DNA, RNA, serta rasio RNA/DNA (p<0,05). Pemberian asam valproat pada induk tikus pada umur kebuntingan 10, 13, dan 16 hari memengaruhi organogenesis otak anak tikus sehingga menyebabkan kerusakan sel-sel saraf penghasil insulin otak yang ditunjukkan oleh  penurunan sekresi dan kadar insulin. [MKB. 2017;49(3):156–64]Kata kunci: Asam valproat, dentate gyrus, insulin, organogenesis Valproic Acid Administration in Pregnant Rats Inhibits Insulin Synthesis n in Brain Cells of the OffspringsValproic acid affects the activity of histone deacetylase which eventually affects gene expression during organogenesis. Insulin plays a role in regulating the proliferation and differentiation of the dentate gyrus. This experiment was designed to study the effects of valproic acid administration on the expression of insulin gene in the dentate gyrus of pregnant rats.  This study was carried out at the Laboratory Animal Management Unit (UPHL) of Bogor Agricultural University  from May 2015 to December 2016, with eighty four newborn rats from control rats and VA-administered rats at the age of 10, 13, and 16 days of pregnancy. The measurements were done for glucose, insulin, DNA, and RNA concentrations in dentate gyrus and to observe the microscopic condition of the brain at the ages of 4 to 32 weeks with 4 weeks interval.  Rats born form mothers administered by 250 mg valproic acid during pregnancy had higher glucose concentrations (p<0.01) and lower insulin concentrations (p<0.05) in the dentate gyrus. During the post-natal growth phase, rats born from mothers administered with valproic acid had increased glucose concentrations and decreased insulin concentrations (p<0.05). Microscopic observations of the dentate gyrus showed degeneration of nerve cells with no immunoreaction to insulin was seen in dentate gyrus cells. However, the concentrations of DNA, RNA, and the ratio of RNA/DNA in the dentate gyrus cells decreased (p<0.05). The administration of valproic acid to pregnant rats at the age of 10, 13, and 16 days of pregnancy destructs  brain organogenesis of the offsprings which eventually disturbs insulin production in the dentate gyrus indicated by decreased insulin secretion and concentrations.  [MKB. 2017;49(3):156–64]Key words: Dentate gyrus, insulin, organogenesis, valproic acid

PENDAYAGUNAAN KALSIUM MEDIA PERAIRAN DALAM PROSES GANTI KULIT DAN KONSEKUENSINYA BAGI PERTUMBUHAN UDANG GALAH (Macrobrachium rosenbergii de Man)

Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol 15, No 2 (2008): Desember 2008
Publisher : Institut Pertanian Bogor

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Abstract

ABSTRAKPenelitian ini dilakukan untuk mengkaji pendayagunaan kapur sebagai sumber kalsium dalam proses peningkatan kadar kalsium kulit dan lama waktu postmolting, serta konsekuensinya bagi pertumbuhan udang. Perlakuan dosis penambahan Ca(OH)2 sebanyak 0 mg/L, 15 mg/L, 30 mg/L, 45 mg/L, dan 60 mg/L, dengan 3 ulangan. Parameter yang diukur meliputi kadar kalsium kulit, lama waktu postmolting, tingkat konsumsi pakan, laju pertumbuhan, dan efisiensi pemanfaatan pakan. Penambahan Ca(OH)2 sebanyak 15-60 mg/L meningkatkan kadar kalsium media (25.51-35.22 mg/L) dibanding dengan kontrol (18.53 mg/L). Pengggunaan Ca(OH)2 sebanyak 0, 15, 30, 45, dan 60 mg/L mampu meningkatkan kadar kalsium kulit pada tahap postmolting 20 hari. Penggunaan Ca(OH)2 sebanyak 30 dan 45 mg/L mampu mempercepat lama waktu postmolting, selanjutnya lebih dari 45 mg/L menghambat lama waktu postmolting. Penggunaan Ca(OH)2 selama 3 siklus kulit berimplikasi lanjut pada konsumsi pakan harian, mulai meningkat pada penambahan Ca(OH)2 15 mg/L, mencapai maksimum pada penambahan Ca(OH)2 45 mg/L, dan selanjutnya menurun pada penambahan Ca(OH)2 60 mg/L. Laju pertumbuhan individu pada penambahan Ca(OH)2 0, 15, 30, 45, dan 60 mg/L adalah 0.006, 0.010, 0.010, 0.012, dan 0.009. Efisiensi pemanfaatan pakan, mencapai maksimal pada penambahan Ca(OH)2 sebanyak 15 mg/L selanjutnya menurun pada 30, 45 dan 60 mg/L. Dengan demikian penggunan Ca(OH)2 sebanyak 30 mg/L mampu mempercepat lama waktu postmolting yang berimplikasi pada peningkatan rataan konsumsi pakan harian sehingga meningkatkan laju pertumbuhan individu udang.Kata kunci: molting, kalsium, konsumsi pakan, pertumbuhan. ABSTRACTThe objectives of these present research was to study the addition of calcium in the media in order to increase the calcium content in the skin and its consequence on the growth of the giant fresh water prawn. Five treatments of different Ca(OH)2 (0, 15, 30, 45, and 60 mg/L), concentration were prepared of which each treatment consisted of three replications. The parameters measured were the concentration calcium of exoskeleton, post molting period, daily feed consumption, total feed consumption, growth rate, and feed efficiency. The addition of 15-60 mg/L has increased the concentration of the media (25.51-35.22 mg/L) compared to the control (18.53 mg/L). Duration of postmolting of the giant freshwater prawns supplemented with 0.15, 30, 45, and 60 mg/L were 17, 15, 12, 13 and 15 days, respectively. The average of daily feed consumptions was found to be higher in the group with the input of Ca(OH)2 of 15 and maximum at 45 mg/L. The growth rate in the prawn suplemented with Ca(OH)2 of 0, 15, 30, 45, and 60 mg/L were 0.006, 0.010, 0.010, 0.12, and 0.009 The feed efficiency in the prawn supplemented with 0, 15, 30, 45, and 60 mg/L were 27.00, 40.45, 30.30, 28.20, and 26.90%. The results of this experiment recomonded that supplementation of 30 mg/L Ca(OH)2 in the aquatic media improved growth rate and feed efficiency of freshwater giant prawn.Keyword: molting, calcium, food consumption, growth.

KINERJA REPRODUKSI TIKUS BUNTING AKIBAT PEMBERIAN EKSTRAK ETANOL PURWOCEN

Jurnal Kedokteran Hewan Vol 8, No 1 (2014): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

Pada penelitian ini, purwoceng diberikan pada tikus betina bunting umur 1-13 hari dengan tujuan mengetahui bobot badan, ovarium, dan uterus serta mempertahankan titik implantasi yang terbentuk sebelum masa implantasi. Penelitian ini menggunakan 2 kelompok yaitu kelompok tikus bunting kontrol dan tikus bunting yang mendapatkan purwoceng dengan dosis 25 mg/300 g bobot badan. Hasil penelitian menunjukkan bahwa kelompok tikus yang diberi purwoceng cenderung memiliki bobot ovarium dan uterus lebih berat dibandingkan kelompok kontrol. Purwoceng juga menyebabkan jumlah titik implantasi tikus hampir mendekati jumlah korpus luteum yang sudah terbentuk, dan ini menunjukkan bahwa keberhasilan implantasi lebih baik pada kelompok tikus yang diberi purwoceng.

Pemberian Asam Valproat pada Induk Tikus Bunting Menghambat Sintesis Insulin pada Sel Otak Anak Tikus

Majalah Kedokteran Bandung Vol 49, No 3 (2017)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Asam valproat memengaruhi aktivitas histone deacetylase yang berperan dalam ekspresi gen selama organogenesis. Insulin berperan dalam proliferasi dan diferensiasi sel-sel saraf dentate gyrus hipokampus. Penelitian ini bertujuan mempelajari pengaruh pemaparan asam valproat pada induk bunting terhadap ekspresi gen insulin pada dentate gyrus. Penelitian dilakukan di UPHL IPB pada bulan Mei 2015 hingga Desember 2016 dengan  84 ekor anak tikus yang dilahirkan oleh induk tikus kontrol yang diberi asam valproat 250 mg pada umur kebuntingan 10, 13, dan 16 hari digunakan untuk pengamatan kadar glukosa, insulin, DNA, RNA, dan rasio RNA/DNA serta pengamatan mikroskopis otak. Pengamatan dilakukan selang waktu empat minggu, dimulai dari umur 4 sampai 32 minggu. Anak tikus yang dilahirkan oleh induk tikus yang diberi asam valproat selama kebuntingan mempunyai kadar glukosa otak yang lebih tinggi (p<0,01) dan insulin yang lebih rendah (p<0,05).  Selama periode pertumbuhan, anak tikus yang dilahirkan oleh induk tikus yang diberi asam valproat mengalami peningkatan kadar glukosa dan penurunan kadar insulin (p<0.05). Pengamatan mikroskopis sel-sel dentate gyrus menunjukkan degenerasi sel dan tidak terlihat reaksi imunoreaktif terhadap insulin, namun terjadi penurunan konsentrasi DNA, RNA, serta rasio RNA/DNA (p<0,05). Pemberian asam valproat pada induk tikus pada umur kebuntingan 10, 13, dan 16 hari memengaruhi organogenesis otak anak tikus sehingga menyebabkan kerusakan sel-sel saraf penghasil insulin otak yang ditunjukkan oleh  penurunan sekresi dan kadar insulin. [MKB. 2017;49(3):156–64]Kata kunci: Asam valproat, dentate gyrus, insulin, organogenesis Valproic Acid Administration in Pregnant Rats Inhibits Insulin Synthesis n in Brain Cells of the OffspringsValproic acid affects the activity of histone deacetylase which eventually affects gene expression during organogenesis. Insulin plays a role in regulating the proliferation and differentiation of the dentate gyrus. This experiment was designed to study the effects of valproic acid administration on the expression of insulin gene in the dentate gyrus of pregnant rats.  This study was carried out at the Laboratory Animal Management Unit (UPHL) of Bogor Agricultural University  from May 2015 to December 2016, with eighty four newborn rats from control rats and VA-administered rats at the age of 10, 13, and 16 days of pregnancy. The measurements were done for glucose, insulin, DNA, and RNA concentrations in dentate gyrus and to observe the microscopic condition of the brain at the ages of 4 to 32 weeks with 4 weeks interval.  Rats born form mothers administered by 250 mg valproic acid during pregnancy had higher glucose concentrations (p<0.01) and lower insulin concentrations (p<0.05) in the dentate gyrus. During the post-natal growth phase, rats born from mothers administered with valproic acid had increased glucose concentrations and decreased insulin concentrations (p<0.05). Microscopic observations of the dentate gyrus showed degeneration of nerve cells with no immunoreaction to insulin was seen in dentate gyrus cells. However, the concentrations of DNA, RNA, and the ratio of RNA/DNA in the dentate gyrus cells decreased (p<0.05). The administration of valproic acid to pregnant rats at the age of 10, 13, and 16 days of pregnancy destructs  brain organogenesis of the offsprings which eventually disturbs insulin production in the dentate gyrus indicated by decreased insulin secretion and concentrations.  [MKB. 2017;49(3):156–64]Key words: Dentate gyrus, insulin, organogenesis, valproic acid