Nurul Khumaida
Departemen Agronomi dan Hortikultura, Fakultas Pertanian IPB Jl. Meranti, Darmaga, Bogor 16680

Published : 64 Documents
Articles

Induksi dan Proliferasi Kalus Embriogenik pada Beberapa Genotipe Kedelai Khumaida, Nurul; Handayani, Tri
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 1 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The successful of gene transformation on crop plants depends on the ability of explant to develop somatic embryos. The effect of medium composition on induction and proliferation of embryonic callus was analyzed on several soybean genotypes, including low irradiance (LI) tolerant genotype (Ceneng, Pangrango C6-30-10 and C6-76-10) and two LI sensitive genotypes (Godeg and Slamet). Immature cotyledons (14 DAA) were cultured in induction medium including MSIA (MS, vitamin B5, 30% sucrose, 0.2% gelrite, 10 mg L-1 2,4-D and 10 mg L-1 NAA) and MSIB (MS, vitamin B5, 30% sucrose, 0.2% gelrite, and 40 mg L-1 2,4-D). Embryonic calli was subcultered one month after initiation onto proliferation medium including MSIIA (MS, vit. B5, 30% sucrose, 0.2% gelrite, 5 mg L-1 2,4-D and 5 mg L-1 NAA) and MSIIB (MS, vitamin B5, 30% sucrose, 0.2% gelrite, and 20 mg L-1 2,4-D). The result showed that percentage of callused explant was 76-94% and the highest initiation obtained on Pangrango genotypes. The average of calli diameter at 1 month after initiation was 0.5-1.2 cm. Calli which was obtained on MSIA medium showed yellow, tranparent, and friable, whereas calli was obtained on MSIB medium showed yellow-brown, transparent, and friable. Increasing calli diameter and structure were obtained on proliferation medium. The combination of 2,4-D and NAA on MSIA and MSIIA both are induction and proliferation medium respectively were better than MSIB and MSIIB which were contain only 2,4-D. Ceneng genotype showed best performance of somatic embryogenesis than others, and Slamet genotype showed lowest response both on callus induction and proliferation.   Keywords: immature cotyledons, embryonic callus, NAA, 2,4
Pengaruh Media Induksi terhadap Multiplikasi Tu nas dan Pertumbuhan Planlet Pisang Rajabulu (AAB) dan Pisang Ta nduk (AAB) pada Berbagai Media Multiplikasi Kasutjianingati, ,; Poerwanto, Roedhy; Widodo, ,; Khumaida, Nurul; Efendi, Darda
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 3 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

 The aim of this research was to study the effects of explant from various induction media on multiplication and growth of cv Rajabulu  (AAB) and Tanduk (AAB) plantain.  This research was arranged in factorial complete randomized design with two factors. The first factor was two kinds of explant which came from induction media I1 (MS + BA 2 mg L-1 + IAA 3 mg L-1), and from induction media I2 (MS + BA 2 mg L-1 + IAA 3 mg L-1 + TDZ 0.09 mg L-1); the second factor was 4 kinds of multiplication media, i.e. MS0 (control/without PGR), MS + BA 0.20 mg L-1 + IAA 0.01 mg L-1 (M1), MS + BA 1 mg L-1 + IAA 0.25 mg L-1 (M2), MS + BA 2 mg L-1 + IAA 0.5 mg L-1 (M3).  The experiment results were the use of TDZ in the induction medium reduced the use of high cytokinin in the multiplication level. The use of Rajabulu explant that came from media I2 produced more shoots (4.3 shoots per explan) compared to explant from media I1 (3.2 shoots per explan). The use of multiplication media M3 and M2 produced the highest shoot number. The best shoot morphogenesis produced when the shoots after subculture in media with PGR (M3 or M2) to media MS0 (big shoot 3.1 and medium shoot 3.5). Tanduk plantain’s shoot was  responsive to cytokinin. The best treatment is I1-M3 with the highest number of shoots and the highest percentage of big and medium shoot is (33%) compared  to  other  treatments  (
Karakter Morfo-Fisiologi Daun, Penciri Adaptasi Kedelai terhadap Intensitas Cahaya Rendah Kisman, ,; Khumaida, Nurul; Trikoesoemaningtyas, ,; Sobir, ,; Sopandie, Didy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 35, No 2 (2007): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The main objective of this study was to identify the leaf morpho-physiological characters as markers for adaptation of soybean to low light intensity. The field experiment was conducted using split plot design with 3 replications. First factor was soybean genotype consisted of shade tolerant genotypes (Ceneng, Pangrango) and sensitive genotypes (Godek, Slamet). Second factor was low light intensity consisted of: L0 = control (under full sun light), L1 = 5 days under 50% shading (exposed after 21 days after planting/DAP), L2 = 5 days under dark condition (exposed after 21 DAP), L3 = 3 days 50% shading + 5 days sun light (exposed after 18 DAP), dan L4 = 3 days 50% shading + 3 days sun light + 5 day dark conditions (exposed after 15 DAP). The 50% shading was made artificially using black plastic paranet with 50% light transmitted. Results of this study showed that under the conditions of low light intensity, leaf morpho-physiological characters of soybean that could be used as markers for adaptation were leaf area, specific leaf weight, and chlorophyll content. Leaves of shade tolerant genotypes were wider and thinner and also contained higher chlorophyll especially for chlorophyll b than that of shade sensitive genotypes under low light intensity.   Key words :  Low light intensity, tolerant genotype, leaf area, chlorophyll, specific leaf weight
Aktivitas Kitinase dan Peroksidase dari Ekstrak Kasar Protein Asal Kalus dan Berbagai Jaringan Tanaman Trichosanthes cucumerina var. anguina Sukma, Dewi; Poerwanto, Roedhy; Sudarsono, ,; Khumaida, Nurul; Artika, I Made; Wiyono, Suryo
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 40, No 3 (2012): JURNAL AGONOMI INDONESIA
Publisher : Bogor Agricultural University

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Abstract

Chitinase and peroxydase are important bioactive proteins or are specific enzymes that are related to plant resistance to pathogens. The aims of the research were to analyze the chitinase and peroxidase activities of crude protein extract from calli, stem, leaves and roots of T. cucumerinavar. anguina. In the first experiment, chitinase and peroxydase activities were analyzed from in vitro calli, leaves and roots obtained from 2-month-old of field grown plants. The media for calli induction were Murashige and Skoog medium with addition of 1 µM NAA + 1 µM BA, 2 µM NAA + 2 µM BA, 3 µM NAA + 3 µM BA, or 4 µM NAA + 4 µM BA. In the second experiment, the chitinase and peroxydase activities from crude protein extract of roots, stems and leaves were analyzed. The extracts were from 3-week-old seedling (less than a month), 1-month and 2-month-old plants. The first and the second experiment results showed that crude protein extracts of plant roots from the field grown plants had the highest chitinase and peroxidase activities. Stem of field grown plants had the similar level of chitinase activities with the plant roots. Chitinase activities of in vitro calli were not significantly different from those of plant roots so that it could be used as an alternative for plant roots in studying chitinase from T. cucumerinavar. anguina. Chitinase activities in crude protein extracts of roots appeared constant whereas peroxidase tend to increase with plant age. Keywords: calli, enzyme activities, leaves, roots, stem
Konservasi In Vitro Pamelo (Citrus maxima (Burm.) Merr.) melalui Pertumbuhan Lambat Tyas, Kartika Ning; Susanto, Slamet; Dewi, Iswari Saraswati; Khumaida, Nurul
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 41, No 1 (2013): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Indonesia is one of the countries which have abundant germplasm of pummelo (Citrus maxima (Burm.) Merr.). Thepummelo germplasm must be conserved to prevent its extinction due to biotic and abiotic stresses. In vitro conservationusing slow growth technique can be considered as an alternative of ex-situ conservation. Two experiments were conductedto obtain suitable medium to conserve pummelo in vitro. The first experiment was conservation using modified concentrationof MS and sucrose. MS medium concentrations were 1/2MS and MS, while sucrose concentrations were 0, 1, 2 and 3%. Thesecond experiment was conservation using osmoticum and retardant in MS medium. There were six combinations of MSmedium supplemented with osmoticum and retardant, i.e. MS + sucrose 3%, MS + sucrose 3% + paclobutrazol 7.5 ppm, MS+ sucrose 3% + paclobutrazol 15 ppm, MS + sorbitol 2%, MS + sorbitol 2% + paclobutrazol 7.5 ppm, MS + sorbitol 2% +paclobutrazol 15 ppm. The results showed that reducing MS medium and sucrose consentration decreased leaf number andshoot length but increased root number and length. The combination of osmoticum and retardant reduced shoot length, leaf number, root number and length. Based on the planlet visual and inhibition of growth through the decrease of leaf number, shoot and root length, the best medium to preserve pummelo was MS + sorbitol 2% + paclobutrazol 7.5 ppm.Keywords: Citrus maxima, osmoticum, preservation, retardant, slow growth
Aktivitas Kitinase dan Peroksidase dari Ekstrak Protein Daun, Akar, Kalus dan Tunas In Vitro Trichosanthes tricuspidata Lour. Sukma, Dewi; Poerwanto, Roedhy; Sudarsono, ,; Khumaida, Nurul; Wiyono, Suryo; Artika, I Made
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 1 (2008): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

A number of Trichosanthes species has been reported as a source of bioactive protein associated with defense mechanisms such as chitinase. Chitinase and peroxidase of crude protein extracted from leaves, roots, in vitro calli and shoots of T. tricuspidata had been analysed. Calli were induced on MS medium containing combinations of 1 µM NAA + 1 µM BA  (K1), 2 µM NAA + 2 µM BA (K2), 3 µM NAA + 3 µM BA (K3), or 4 µM NAA + 4 µM BA (K4). Shoots were cultured in MS with 1 mg/l of BA, while leaves and roots were harvested from six-month old plants grown on the field. Results of the experiment suggested that K1-K4 medium could be used to induce calli although weight of calli from all medium composition  was not significantly different (0.19-0.31 g/explant/4 weeks). Calli from K1 medium had the highest of total crude protein content (3.24 mg/ml). The highest of chitinase activity was found in in vitro shoots (6.51 mM pNP/hour/mg protein) and the highest peroxidase activity was in the plant roots (0.25 ∆ 420/minute/mg protein).   Key words:  in vitro calli, shoots, crude protein, chitinase and peroxidase activities
Pola Pewarisan Adaptasi Kedelai (Glycine max L. Merrill) terhadap Cekaman Naungan Berdasarkan Karakter Morfo-Fisiologi Daun Kisman, ,; Trikoesoemaningtyas, ,; Sobir, ,; Khumaida, Nurul; Sopandie, Didy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 1 (2008): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The objective of this study was to analyze the inheritance pattern of soybean adaptation to shade stress based on leaf morpho-physiological characters. Genetic materials used in this study consisted of 22 plants of low irradiance (LI)-tolerant genotype (Ceneng) and 22 plants of LI-sensitive genotype (Godek); 21 plants of F1 (Ceneng x Godek); and 114 plants of F2 populations (derived from F1). These populations were planted under shading of paranet 50%. The population was arranged in a Randomized Complete Block Design with two replicates. Analysis of inheritance of soybean adaptation involved estimation of heritability (broad sense) and gene action. Results of this study showed that: adaptation of soybean to low light stress based on characters of yield per plant was highly heritable (68% of broad sense) with partial dominant mode of action. Characters of leaf area and specific leaf weight were highly (68% of broad sense) and moderately (48% of broad sense) heritable, respectively, with additive mode of action. Soybean adaptation based on leaf physiological characters (chlorophyll contents) was highly heritable (70% - 86% of broad sense) in epistatic mode of action.   Key words:  Soybean, leaf morpho-physiological character, heritability, gene action
Perbaikan Teknik Kultur Embrio Kelapa Kopyor (Cocos nucifera L.) Asal Sumenep Jawa Timur Melalui Penambahan Bahan Aditif dan Pengujian Periode Subkultur Sukendah, ,; Sudarsono, ,; Witjaksono, ,; Khumaida, Nurul
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 1 (2008): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The success of culturing of "Kopyor" coconut (matured coconut with broken meat particles due to abnormal formation of endosperm) through embryo culture depends on the medium used.  A revised protocol on medium embryo culture was done to increase embryo germination and plantlet production of kopyor coconut obtained from Sumenep.  Embryos excised from mature nuts were cultured in solid Eeuwens basal media supplemented with 150 ml coconut water, 150 ml coconut milk, 50 mg/l thio-urea, and 100 mg/l thio-urea. Germinating embryos were transferred to solid Eeuwens basal media containing 100, 150, and 200 ml/l coconut water.  Subsequent transfers of the germinating embryos to fresh media to complete seedling development were done at different periods of subculture, i.e., 1, 2, 3, and 4 months. The highest viability of kopyor embryo was shown by 150 ml/l coconut water, about 95% embryos in this medium germinated. At plantlet phase, addition of coconut water did not give a positive result to the growth of kopyor plantlets.  However, coconut water could increase the complete plantlet, i.e., plantlet with shoot and good root. Growth and number of kopyor plantlets obtained were the best when the plantlets transferred into fresh medium every 2 months.   Key words:  Embryo culture, kopyor coconut, additive agents, period of subculture
Karakterisasi Sekuen Lengkap JJ3 yang Diisolasi dari Kedelai Toleran Naungan Khumaida, Nurul; Kisman, ,; Sopandie, Didy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 2 (2008): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The aim of this study was to characterize full length sequence of JJ3 isolated from shade tolerant soybean. The characterization of full length sequence involved analyses of structure of full length sequence, open reading frame (ORF) or coding sequence (CDS), and homology. The analyses of JJ3 full length sequence structure were done using GeneScanW program, coding sequence (CDS) or open reading frame (ORF) using ORF Finder program, and the homology of JJ3 full length sequence were done using BLAST and clustalW programs. Results showed that the full length sequence of JJ3 consisted of single exon and polyA. Single exon was a coding sequence (CDS) 633 bp produced open reading frame (ORF) with 210 amino acid deduction comprised of 136 conserved amino acids and 77 varied amino acids. The conserved domain of JJ3 produced functional protein, PsaD photosystem I (PSI) subunit, related to photosynthetic transport electron in PSI reaction center. The full length of JJ3 homolog to psaD, a gene encoding protein subunit photosystem I (PSI) related to mechanism of plant adaptation to shade stress, in wood tobacco (Nicotiana sylvestris), rice (Oryza sativa), tomato (Lycopersicon esculentum), barley (Hordeum vulgare), spinach (Spinacia oleracea), Arabidopsis (Arabidopsis thaliana), and potato (Solanum tuberosum).     Key words: full length sequence, gene, homology, shade tolerant, soybean
Efektifitas Aplikasi In-vitro Rizobakteri Sebagai Agen Antagonis Layu Fusarium pada Pisang Rajabulu/AAB di Rumah Kaca ., Kasutjianingati; Poerwanto, Roedhy; ., Widodo; Khumaida, Nurul; Efendi, Darda
Jurnal Hortikultura Indonesia Vol 2, No 1 (2011): Jurnal Hortikultura Indonesia
Publisher : Perhimpunan Hortikultura Indonesia

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Abstract

Research was conducted on a banana cultivar of Rajabulu/AAB inside the green house. This research was aimed to study the effect of rhizobacteria and the ability of in vitro bacterization to prevent FOC. This study was conducted using split plot design. The main plot was rhizobacteria treatments (P. fluorescens ES-32, B. subtilis SB-3, a mix of both types and without bacteria), sub plots consist of in vitro (2 weeks and 1 week prior to application) and in vivo (during acclimatization) bacterization. All treatments were repeated 3 times, each consist of 7 polibags. The result of rhizobacteria treatment (B. subtilis SB3 or P. fluorecens ES32) was able to reduce the Disease Severity (Rhizome Discoloration) caused by Fusarium oxysporum f.sp cubense (FOC). The incorporation of mixed rhizobacteria provided significant effect suppressing the infestation of FOC. The application of mix bacteria was better than single treatment.Keywords: B. subtilis, P. fluorecens, rhizome discoloration, fusarial wilt, suppressing
Co-Authors , Kasutjianingati , Kisman , Sobir , Sudarsono , Sukendah , Trikoesoemaningtyas , Widodo , Witjaksono . TRIKOESOEMANINGTYAS ACHMAD . ADI SETIADI AGUS PURWITO Ahmad Rifqi Fauzi AMIN NUR Aniversari Apriana Ardie, and Sintho Wahyuning Ardie, dan Sintho Wahyuning Ardie, dan Sintho Wahyuning Ardie, dan Sintho Wahyuning Arrin Rosmala, Arrin Atmitri Sisharmini Bambang S. Purwoko Bambang Sapta Purwoko Bhayu Hartanti Bintang, dan Maria Buang Abdullah Cucu Sukmana, Cucu Darda Efendi Dede J. Sudrajat Dede Jajat Sudrajat, Dede Jajat Dewi Sartiami DEWI SUKMA DIDY SOPANDIE Diny Dinarti Effendy, Jollanda Enny Sudarmonowati Firdausya, Andina Fabrini Gustaaf A Wattimena, Gustaaf A Gustaaf Adolf Wattimena I MADE ARTIKA Ika Mariska Ika Roostika IRDIKA MANSUR Iskandar Z. Siregar Iskandar Z. Siregar Iswari S Dewi Iswari S. Dewi Iswari Saraswati Dewi Kartika Ning Tyas Kasutjianingati . Kisman Kisman Kurniawan R. Trijatmiko Kurniawan Rudi Trijatmiko, Kurniawan Rudi La Muhuria Lefin Kafindra, Lefin Lola Adres Yanti, Lola Adres MARTIANSYAH, Irfan Muaz Adbdul Karim MUHAMAD SYUKUR Muhammad Syukur MUNIF GHULAMAHDI N. Sri i Hartati Natalini Nova Kristina Nugroho, Candra Catur Nura ,, Nura Nurul Fauziah Pienyani Rosawanti, Pienyani PUTRANTO, Riza Arief Putranto, Riza Arief Rahmiati, Tengku Mia Reny Herawati ROEDHY POERWANTO Ryan Budi Setiawan, Ryan Budi Sadewi Maharani, Sadewi Saepudin, Adam Saepudin, Adam Sintho Wahyuning Ardie Sintho Wahyuning Ardie, Sintho Siti Kurniawati Slamet Budijanto Slamet Susanto Subekti, Isnani SUDARSONO SUDARSONO SUDIRMAN YAHYA SURYO WIYONO Syukur, dan Muhamad Tri Handayani Tri Lestari Mardiningsih, Tri Lestari Trijatmiko, dan Kurniawan Rudi Trikoesoemaningtyas, dan Ulfah J. Siregar Ulfah J. Siregar, Ulfah Wahyuning Ardie, dan Sintho Waras Nurcholis WARID, WARID Wattimena, and Gustaaf Adolf Wening Enggarini Widodo , Widodo . Widowati, Sartika Yani, Rahmi Henda Yohanes Aris Purwanto Yudiansyah Yudiansyah