ANDI KHAERUNI
Jurusan Agroteknologi, Faperta, Universitas Halu Oleo

Published : 13 Documents
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Rapid Detection of Bacterial Pustule Disease on Soybean Employing PCR Technique with Specific Primers

HAYATI Journal of Biosciences Vol 14, No 2 (2007): June 2007
Publisher : Bogor Agricultural University, Indonesia

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Abstract

A rapid polymerase chain reaction (PCR)-based procedure was developed for detection of Xanthomonas axonopodis pv. glycines, the causal agent of bacterial pustule disease on soybean. A set of primers was designed from partial sequence of the pathogenicity gene of X. axonopodis pv. glycines strain YR32. Specific PCR product of 490 base pairs was produced from strains of X. axonopodis pv. glycines originally from Indonesia as well as from Taiwan. No other pathovars and bacterial species among those tested showed amplification product under optimized PCR conditions. Shaking infected soybean leaves in phosphate buffer saline during six hours was proved to be an essential in order to increase cell number of the bacterial. The procedure was applicable and reliable for detecting of pathogens in infected plant materials. The procedure was proved to be more effective than that of conventional detection and could be of great help for monitoring of pustule bacterial disease in the soybean fields. Key words: Xanthomonas axonopodis pv. glycines, bacterial pustule disease, rapid detection, PCR, specific primer

Penggunaan Bakteri Kitinolitik sebagai Agens Biokontrol Penyakit Busuk Batang oleh Rhizoctonia solani pada Tanaman Kedelai (Utilization of Chitinolitic Bacteria as Biocontrol Agent of Stem Rot Disease by Rhizoctonia solani on Soybean)

Jurnal Fitopatologi Indonesia Vol 8, No 2 (2012)
Publisher : Jurnal Fitopatologi Indonesia

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Abstract

Biological control of stem rot disease caused by Rhizoctonia solani on soybean using chitinolitic bacteria is one of the environmentally friendly control methods of pathogen. The study was conducted to select chitinolitic bacteria and to test their ability to suppress stem rot disease and promote plant growth of soybean. A total of ten chitinolitic bacteria were tested in vitro, and five of isolates i.e: ST21e, SS12b, ST17c, ST27d, and ST26c found to have more than 30% suppression toward R. solani. In planta test under green house condition at six weeks after planting showed that soybean plants inoculated with ST27d and ST17c isolates had significantly lower disease incidence and had increased plant height and leaf number. Both of chitinolitic bacteria isolates are promising as biocontrol agents of stem rot disease caused by R. solani of soybean.Key words: biological control, chitinolitic bacteria, Rhizoctonia solani

Aplikasi Formula Campuran Rizobakteri untuk Pengendalian Penyakit Busuk Akar Rhizoctonia dan Peningkatan Hasil Kedelai di Tanah Ultisol

Jurnal Fitopatologi Indonesia Vol 10, No 2 (2014)
Publisher : Jurnal Fitopatologi Indonesia

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Abstract

Rhizoctonia root rot disease caused by Rhizoctonia solani is one of the most important disease in soybean area, including at ultisol land in Southeast Sulawesi. Rhizobacteria application is one alternative method to control this pathogen. The aim of this experiment was to study of rhizobacteria indigenous formulation to control of Rhizoctonia root rot disease and increase soybean yield in Ultisol soil. A complete randomized design with seven treatments was used in this experiment. The treatments were (A) rhizobacteria formulation for seed treatment, (B) rhizobacteria formulation for seed treatment and repeated at 2 weeks after planting, (C) rhizobacteria formulation for seed treatment, repeated at 2 and 4 weeks after planting, (D) rhizobacteria formulation for seed treatment and fungicide applied at 2 weeks after planting, (E) fungicide seed treatment, and repeated at 2 weeks after planting, (F) fungicide seed treatment, and repeated at 2 and 4 weeks after planting, and control (without rhizobacteria and fungicides). All treatments were inoculated by R. solani and replicated three times. The results showed that rhizobacteria seed treatment and repeated at 2 and 4 weeks after planting was the most effective treatment to control Rhizoctonia root rot disease, and increase plant height and leaf number up to 119% and 170%, respectively, and increased the yield of soybean up to 1870% in ultisol soil compared to plant with control treatment.  

Perkembangan Penyakit Hawar Daun Bakteri pada Tiga Varietas Padi Sawah yang Diinokulasi pada Beberapa Fase Pertumbuhan

Jurnal Fitopatologi Indonesia Vol 10, No 4 (2014)
Publisher : Jurnal Fitopatologi Indonesia

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Abstract

Bacterial leaf blight is an important disease of rice plant and could damage up to 50%. This study aimed to evaluate development of the bacterial leaf blight disease on three rice varieties which inoculated at various growth stage. The results of this study showed that IR64 variety which inoculated at seedling stage has shortest incubation period as well as disease severity i.e. 4.25 day after inoculation and 90%, respectively, while Cisantana variety which inoculated at generative stage showed the lowest of disease severity (< 40%). The highest number of panicles obtained on without inoculation treatment i.e an average of 10 panicles. Therefore growth stage and rice variety influenced to bacterial leaf blight disease development, the younger the plant infected, the faster the progression of the bacterial leaf disease. IR64 variety is highly susceptible to bacterial leaf blight disease. 

Integrasi Teknik Invigorasi Benih dengan Rizobakteri untuk Pengendalian Penyakit dan Peningkatan Hasil Tomat

Jurnal Fitopatologi Indonesia Vol 10, No 6 (2014)
Publisher : Jurnal Fitopatologi Indonesia

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Abstract

The effects of pre-planted seed invigoration treatment integrated with rhizobacteria were studied in an attempt to control fusarium wilt disease and to improve yield of tomato in the field. Seed treatments consisted of seed invigoration with single or mixture rhizobacteria, burned rice husk or sawdust seed matriconditioning, integrated seed matriconditioning with single or mixture rhizobacteria, seed hydration, and fungicide seed treatment. The result showed that the most effective treatment was the integration of rice hull charcoal seed matriconditioning with Bacillus polymixa BG25. The seed treatment increased total fruit and fruit weight by 40% and 166% , respectively. The treatment was also effective in suppressing disease incidence by 70%. Based on this experiment, rhizobacteria B. polymixa BG25 integrated with rice hull charcoal seed matriconditioning can be recommended as biocontrol agents in improving yield and controlling fusarium wilt disease of tomato. 

DETEKSI POTYVIRUS PADA NILAM (Pogostemon Cablin (BLANCO) BENTH) DENGAN TEKNIK ELISA DI SULAWESI TENGGARA

Jurnal Agroteknos Vol 4, No 1 (2014)
Publisher : Jurnal Agroteknos

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Abstract

Mosaic symptoms were observed on Patchouli (Pogostemon cablin) around North Kolaka and Kolaka, Southeast Sulawesi during surveys in early 2012. Indirect-ELISA based detection was conducted using symptomatic leaf samples. The objective of the research was to determine disease incidence of Potyvirus in several farms of Patchouli plant in Southeast Sulawesi. The results showed that Patchouli plant (Pogostemon cablin) was found to be infected with Potyvirus disease showing mosaic symptoms and malformation on the leaf samples i.e. in Amotowo and Boro-Boro Villages of subdistrict Boro-Boro, and Landabaro Village, Mowila subdistrict of South Konawe regency; Asinua Village of subdistrict Unaaha, Lambuya district of subdistrict Lambuya, and Bungguosu district, Konawe subdistrict of Konawe regency; and Anduonohu district, Poasia subdistrict of Kendari regency. This is the first report on Potyvirus infection on patchouli in Southeast Sulawesi. Keywords: Inderect-ELISA, mosaic, Potyvirus, Pogostemon cablin

EKSPLORASI DAN KARAKTERISASI AZOTOBACTER INDIGENOUS UNTUK PENGEMBANGAN PUPUK HAYATI TANAMAN PADI GOGO LOKAL DI LAHAN MARJINAL

Jurnal Agroteknos Vol 4, No 2 (2014)
Publisher : Jurnal Agroteknos

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Abstract

Azotobacter is bacterium that has the ability to fix nitrogen and produce hormone IAA, thus potentially acts as biofertilizer agent. There are differences in chemical, biological and other characters of Azotobacter strains. Some strains have higher ability to fix nitrogen than the others. Exploration and characterization are important to be done because the bacteria that provide nutrients and live free rhizosphere regions, possible have different abilities.  In addition to N2 fixing, growth hormone production, phosphate dissolving, they are also tolerant to a certain temperature and pH. The purpose of the research was to obtain information and the latest data on indigenous Azotobacter that potential as a source of biofertilizer, and triggering factor for upland rice growth on marginal lands in Southeast Sulawesi.  Exploration from several locations has selected 21 Azotobacter isolates. The test results indicated that the 21 Azotobacter isolates have the ability to produce IAA, dissolve phosphate and stable at pH 5.0-7.0. All 21 isolates tested had the ability to survive at 40oC, eight isolates i.e. LT2D1, LT2d2, LU2c, RG4c, MP1f, LT2d3, ML1j, and RR8awere able to survive at a temperature of 45o C, and LT2d1 isolate survived at temperatures 50oC. The results of the evaluation of the wet weight of upland rice seedlings selected 10 isolates that were: KU6e, MS3e, RG4c, RR8b1, LU2c, RB4b, MS3f, LU2c1, RJ5e, RR8b2 and evaluation of seedling dry weight selected 5 isolates that were: RB4b, LU2c, RJ5e, RR8b2, LT2d1. Keywords:      exploration, characterization, indigenous Azotobacter, local upland rice

ISOLASI DAN KARAKTERISASI BAKTERI MANOLITIKASAL BONGGOL POHON SAGU

Jurnal Agroteknos Vol 4, No 3 (2014)
Publisher : Jurnal Agroteknos

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Abstract

Sago Processing in Southeast Sulawesi often generate waste in the form of pulp and tuberswhich contains lignin, cellulose, starch, minerals, and vitamins that can be used as a source of carbon and energy for growth of microorganisms, so it is likely to get microbes, including mannolitic bacteria that are useful for human life. The aim of this study was to isolate and characterize the biochemical properties of mannolitic bacteria originated fromwaste of sago hump in Konawe, Southeast Sulawesi Province. Isolation was done using serial dilution method and then spread over the surface of Nutrient agar medium and Mannan enrichment. Bacterial isolates showing high mannose activity were characterized mannose physiologically and biochemically. From this research, 6 mannolitic isolates originated from hump of sago waste samples from South Konawe were obtained. BLS.11-01 and BLS.11-02 mannolitic bacterial isolates had a strong mannolitic activity, with mannolitic index value of 2.3 and 2.0, respectively. Presumably, the two isolates were gram-positive bacteria, belonging to the same genus.

Keragaman Genetika Xanthomonas axonopodis pv. glycines Asal Kedelai Varietas Edamame di Indonesia

Jurnal Perlindungan Tanaman Indonesia Vol 13, No 1 (2007)
Publisher : Universitas Gadjah Mada

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Abstract

Xanthomonas axonopodis pv. glycines cause bacterial pustule disease caused a serious disease in Edamame cultivation in Indonesia. We collected a total of 29 X. axonopodis pv. glycines isolates from Edamame fields at Jember, Ciawi, Cipanas and Bogor. The genetic diversity analysis of all isolates employing ARDRA and ISR technique showed six and seven different DNA profile, respectively. Therefore there are at least seven strains of X. axonopodis pv. glycines infected Edamame in Indonesia. Both CPI from Cipanas and JA4 from Sukorejo Jember isolates possess unique DNA profle and genetically are not closely related to other isolates.

Keefektifan Waktu Aplikasi Formulasi Rizobakteri Indigenus untuk Mengendalikan Layu Fusarium dan Meningkatkan Hasil Tanaman Tomat di Tanah Ultisol

Jurnal Hortikultura Vol 23, No 4 (2013): Desember 2013
Publisher : Indonesian Center for Horticulture Research and Development

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Abstract

Layu fusarium merupakan salah satu penyakit penting di pertanaman tomat, termasuk di lahan Ultisol di Sulawesi Tenggara. Rizobakteri pemacu pertumbuhan tanaman merupakan salah satu alternatif pengendalian penyakit yang menjanjikan untuk mengatasi masalah layu fusarium. Penelitian bertujuan mengetahui keefektifan formulasi rizobakteri indigenus untuk mengendalikan layu fusarium dan meningkatkan hasil panen tanaman tomat di tanah Ultisol. Penelitian dilaksanakan di Laboratorium Agroteknologi dan Rumah Kasa, Fakultas Pertanian, Universitas Halu Oleo dari Bulan Oktober 2010 sampai Januari 2011. Penelitian disusun berdasarkan rancangan acak lengkap dengan tujuh perlakuan, yaitu (RB) aplikasi rizobakteri pada benih, (RB.2) aplikasi rizobakteri pada benih dan pada tanaman 2 minggu setelah tanam, (RB.2.4) aplikasi rizobakteri pada benih dan pada tanaman 2 dan 4 minggu setelah tanam, (RBF.2) aplikasi rizobakteri pada benih dan aplikasi fungisida sintetik pada tanaman 2 minggu setelah tanam, (FB.4) aplikasi fungisida sintetik pada benih dan pada tanaman 2 minggu setelah tanam, (FB.2.4) aplikasi fungisida sintetik pada benih dan pada tanaman 2 dan 4 minggu setelah tanam, serta kontrol. Semua perlakuan diinokulasi dengan Fusarium oxysporum f. sp. lycopersici dan diulang tiga kali. Hasil penelitian menunjukkan bahwa perlakuan rizobakteri pada benih yang disusul pada saat 2 dan 4 minggu setelah tanam paling efektif mengendalikan layu fusarium dengan penekanan sebesar 61,14%, serta mampu meningkatkan hasil tanaman dengan jumlah buah 10,88 dan bobot buah 375,31 g per tanaman, sedangkan tanaman tanpa perlakuan tidak berproduksi. Hasil ini mengindikasikan bahwa formulasi rizobakteri indigenus mampu mengendalikan layu fusarium dan meningkatkan hasil panen tanaman tomat di tanah Ultisol.