Musliar Kasim
Department of Agroecotechnology, Faculty of Agriculture Andalas University, Padang, Indonesia

Published : 7 Documents
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BIODIVERSITY OF ARBUSCULAR MYCHORRIZAL FUNGI (AMF) ON POTATOS RHIZOSPHERE AND IT POTENTIAL AS BIOFERTILIZER

Sainstek Vol 12, No 1 (2009): Vol. XII No. 1, September 2009
Publisher : Lembaga Penelitian Universitas Negeri Padang

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Abstract

AMF as the biofertilizer on several crops  has been reported, but not so much the information about biodiversity of AMF on potatos rhizosphere and its potential to colonize that crop. The research about the biodiversity of AMF has been done in laboratorium  Biology in Faculty of Agriculture of Andalas University and in laboratorium Biotecnology of Agriculture Faculty of Jambi University from November 2004 till April 2005. The sample of soil have been collected from  rhizosphire of potatos plant in Alahan Panjang, West Sumatera, and then the AMF spore are isolated and identification based on morphology and size of spores. Inoculation of single and multi spores to potatos root to see the structure of colonization and percentage of colonization. The result of identification of AMF spores show that there are many kind of spores on rhizosphere of potatos plant and the spores are dominated by: Glomus, Acaulospora, Scutellospora, Gigaspora, and Enterophospora. Unfortunately, the inoculation of single spore has no good effect to the structure of colonization and the potatos plant not vigorous and easy to be severe of stem desease. But, inoculation with multi spores show that the tipe of colonization on potatos root have the coil hyphal and intracellular vesicular. The same result also indicated that colonization with active propagule (mycelium, spores, and infected root) have the coil hyphal and intracellular vesicular. The species of AMF that have the typical characteristic is assumed as Gigaspora sp.. Key words :    AMF, potatos rhizosphere, biofertilizer

Karakteristik Morfologi dan Genetik Tanaman Penghasil Gaharu (Aquilaria spp) Endemik Sumatera Barat

Sainstek Vol 11, No 1 (2008): Vol. XI No. 1, September 2008
Publisher : Lembaga Penelitian Universitas Negeri Padang

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Abstract

Agarwood (Aquilaria spp) is plant of tha important tropical forest trees,which produces a high economically valuable fragrant resinous wood. The objective of this  study was to characterize  the agarwood based on  morphologist are  genetic distance using RAPD markers. Characterizeof the agarwood based on morphologist and genetic distance by using RAPD markers is on alternative method to see variance morphologist,genetic and condition population plant agarwood (Aquilaria sp) endemic West Sumatra. This experiment was conducted at rain forest West Sumatraand  Laboratory Agronomy Agriculture faculty Andalas University, and Laboratory Biotecnology Seameo Biotrop Bogor, during mar 2006 toDecember 2007.The result showed two group mayor cluster to agarwood i.e. Aquilaria malacensis  and Aquilaria microcarpa endemic West Sumatra by using fenotipe variabilities and RAPD markers. Broad genetic variabilties were found for long and wide leaf width. Keyword : morfologist, genetic, Agarwood, and RAPD.

PENGARUH KETINGGIAN TEMPAT DAN MUSIM TERHADAP FLUKTUASI APHID PADA TANAMAN KENTANG

Jurnal Sagu Vol 1, No 01 (2002)
Publisher : Jurnal Sagu

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Abstract

The study on the innuence of altitude and season toward aphid population fluctuation inpotato plant. This research was carried out in Rimbo Data, Aia Butunibuak and Sukaraniiareas. Aphid identification was done in Laboratory of Agronomy Study Program, Faculty ofAgriculture, Andalas University, Padang, and in the Research Hall of Fruit Plant, Solok. Theaphid population fluctuation was observed by capturing the aphid flying by installing threetraps of Moerickc tray put in the potato plantation in each area. Treatment of this researchwas in the difference of position altitude of the capture area, viz, Rimbo Data (1,700 mDPL), Aia Batunibuak (1.350 m DPL), and Sukaranii (1.000 in DPL). The research resultshowed that the aphid population in the potato plantation was lower in the area of RimboData (1.700 m DPL) compared with the area of Aia Baturnbuak (1,350 m DPL) and Sukarami(1.000 m DPL). The period witliout aphid was only found in the area of Rimbo Data atOctober, and January, The type of dominant aphid in Rimbo Data was Macrosiphumeiiphorbiae, in Aia Baturnbuak and Sukaranii were Aphis fabae.

Peningkatan Kapasitas Sink pada Tanaman Melon (Cucumis melo L.) dengan Pemberian Giberelin

Jurnal Sagu Vol 8, No 02 (2009)
Publisher : Jurnal Sagu

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Abstract

For cultivation of melon (Cucumis melo L.), farmers only keep one fruit per plant to maintain quality fruit. To improve productivity, one possible effort is by spraying gibberellins at melon in combination with increasing amount of fruit maintained for each plant. The aim of this study was to know the effect of gibbereline in improving sink capacities of melon when fruit amount is increased. Gibberelins was sprayed at concentration of 0, 30, 60, and 90 mg/l to melon crop with different fruit amount per plant (one, two and three fruit). The study indicated that for melon plant with more than one fruit per plant, gibberellins application was not able to maintain the fruit quality, because the fruit weights were still decreasing. Thus, gibberellins application was not able to improve the sink capacities if fruit amount per plant is increased. While for plant with only one fruit, spraying 60 and 90 mg/l gibberellins can improve the fruit weight, so gibberellins improves the sink capacities if only one fruit is maintained for each plant.

Kloning dan karakterisasi gen penyandi inhibitor proteinase dari kulit buah kakao Cloning and characterization of gene encoding proteinase inhibitor of cacao pod wall

E-Journal Menara Perkebunan Vol 76, No 2: Desember 2008
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

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Abstract

Summary Attempts to increase cocoa production in Indonesia have been hinderred by attack of CPB (Conopomorpha cramerella). There has been no effective measures to control this pest leading to development of cacao planting materials which resistant to the pod borer. One of genes functioning in plant defense system against insect pests such as catepilar is Proteinase Inhibitor (PIN). This research aimed to isolate and characterize TcPIN gene of cacao pod wall. A clone of TcPIN was isolated with RT-PCR technique using total RNA of cacao pod wall and DNA primer designed based on the sequence Trypsin Inhibitor of cocoa bean accessible online. BlastX analysis of the sequence of the cDNA clone demonstrated that the ± 600 bp gene cloned with pGEM-T was PIN gene as indicated by highly homologous to Trypsin Inhibitor of Theobroma microcarpum resulted in 248 Score bits and E value 1 e-64. Two sequence alligment with the putative 21 kDa PIN  of cacao seed indicated a moderately high homology. Contrasting these two sequences however found some non identical amino acids implying some variations. Ringkasan Usaha peningkatan produksi kakao di Indonesia terkendala antara lain oleh adanya serangan hama PBK (Conopomorpha cramerella). Untuk menanggulangi serangan PBK tersebut perlu adanya satu cara pengendalian yang efektif dan efisien, sehingga dapat mendorong usaha pengembangan bahan tanam yang tahan PBK. Salah satu gen  membawa sifat ketahanan tanaman terhadap hama ulat adalah Proteinase Inhibitor (PIN). Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi gen TcPIN dari kulit buah kakao. Klon cDNA TcPIN diisolasi dari kulit buah kakao dengan teknik RT-PCR meng-gunakan RNA total kulit buah kakao dan primer DNA yang dirancang atas dasar sekuen Inhibitor Tripsin biji kakao yang diakses lewat internet.  Hasil analisis BlastX dari sekuen klon cDNA menunjukkan  bahwa gen berukuran  ± 600 pb yang telah diklon dengan pGEM-T tersebut adalah PIN karena memiliki homologi yang tinggi terhadap 21 kDa trypsin inhibitor dari Theobroma microcarpum yang meng-hasilkan Skor 248 bits dengan Nilai E 1e-64. Penjajaran dua sekuen dengan PIN putatif 21 kDa yang berasal dari biji kakao menunjuk-kan tingkat homologi yang tinggi, dengan perbedaan nyata sehingga dapat terlihat bahwa keduanya tidak identik.

Kloning dan karakterisasi gen penyandi inhibitor proteinase dari kulit buah kakao Cloning and characterization of gene encoding proteinase inhibitor of cacao pod wall

E-Journal Menara Perkebunan Vol 76, No 2: Desember 2008
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Original Source | Check in Google Scholar | Full PDF (335.457 KB)

Abstract

Summary Attempts to increase cocoa production in Indonesia have been hinderred by attack of CPB (Conopomorpha cramerella). There has been no effective measures to control this pest leading to development of cacao planting materials which resistant to the pod borer. One of genes functioning in plant defense system against insect pests such as catepilar is Proteinase Inhibitor (PIN). This research aimed to isolate and characterize TcPIN gene of cacao pod wall. A clone of TcPIN was isolated with RT-PCR technique using total RNA of cacao pod wall and DNA primer designed based on the sequence Trypsin Inhibitor of cocoa bean accessible online. BlastX analysis of the sequence of the cDNA clone demonstrated that the ± 600 bp gene cloned with pGEM-T was PIN gene as indicated by highly homologous to Trypsin Inhibitor of Theobroma microcarpum resulted in 248 Score bits and E value 1 e-64. Two sequence alligment with the putative 21 kDa PIN  of cacao seed indicated a moderately high homology. Contrasting these two sequences however found some non identical amino acids implying some variations. Ringkasan Usaha peningkatan produksi kakao di Indonesia terkendala antara lain oleh adanya serangan hama PBK (Conopomorpha cramerella). Untuk menanggulangi serangan PBK tersebut perlu adanya satu cara pengendalian yang efektif dan efisien, sehingga dapat mendorong usaha pengembangan bahan tanam yang tahan PBK. Salah satu gen  membawa sifat ketahanan tanaman terhadap hama ulat adalah Proteinase Inhibitor (PIN). Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi gen TcPIN dari kulit buah kakao. Klon cDNA TcPIN diisolasi dari kulit buah kakao dengan teknik RT-PCR meng-gunakan RNA total kulit buah kakao dan primer DNA yang dirancang atas dasar sekuen Inhibitor Tripsin biji kakao yang diakses lewat internet.  Hasil analisis BlastX dari sekuen klon cDNA menunjukkan  bahwa gen berukuran  ± 600 pb yang telah diklon dengan pGEM-T tersebut adalah PIN karena memiliki homologi yang tinggi terhadap 21 kDa trypsin inhibitor dari Theobroma microcarpum yang meng-hasilkan Skor 248 bits dengan Nilai E 1e-64. Penjajaran dua sekuen dengan PIN putatif 21 kDa yang berasal dari biji kakao menunjuk-kan tingkat homologi yang tinggi, dengan perbedaan nyata sehingga dapat terlihat bahwa keduanya tidak identik.

TANTANGAN PENGEMBANGAN PADI DIKABUPATEN KEPULAUAN MENTAWAI

Jurnal AGRISEP JURNAL AGRISEP VOL 16 NO 1 2017
Publisher : Prodi Agribisnis Jurusan Sosial Ekonomi Pertanian Fakultas Pertanian Universitas Bengkulu

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Abstract

This study aims to identify the problems and develop rice agribusiness in Mentawai Islands from upstream to downstream and formulate appropriate policy recommendations to be implemented. The research location determined by purposive namely in the village Makalo and Malakopa District of South Pagai, Sikakap subdistrict Taikako Village, Village Saumanganya District of North Pagai, Bosua Village and Village Beriulou District of South Sipora, Village Rogdog and Madobag District of South Siberut. Respondents in this study were 30 farmers. Respondents farmers selected by simple random sampling method. The results showed that the majority of rice paddies new openings in six districts consist of peatlands with a depth varying from 0.4- > 2.0 meter, fields new openings that have been implemented in the Mentawai Islands in general can not be expected to result in maximum productivity due to problems of land suitability diverse. Farmers also do not take action appropriate technical culture starting from land preparation, seed selection, weeding, fertilizing and water management. Behaviorally agriculture, farmers in Mentawai Islands is not a society that blends with the culture of rice fields, it is because basically their agricultural base is dry land agriculture with farming patterns. This will certainly give a great challenge and not easy for the cultivation of rice in the Mentawai Islands.Keywords:challenge,rice agribusiness, cultivation of rice