Rina Sri Kasiamdari
Laboratorium Sistematika Tumbuhan, Fakultas Biologi Universitas Gadjah Mada Jl. Teknika Selatan, Sekip utara, Daerah Istimewa Yogyakarta

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Karakter Makromorfologi dan Mikromorfologi Duku, Kokosan, Langsat dalam Penentuan Status Taksonomi pada Kategori Infraspesies Hanum, Laila; Kasiamdari, Rina Sri; Santosa, Santosa; Rugayah, Rugayah
Biospecies Vol 6, No 02 (2013)
Publisher : Biospecies

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Abstract

Duku, kokosan dan langsat merupakan buah-buahan yang populer dan memiliki nilai ekonomi yang penting. Secara morfologi duku, kokosan dan langsat  hampir mirip satu sama lain sehingga menyulitkan dalam penentuan pada tingkat infraspesies bagi para pemulia tanaman. Berbagai pendekatan untuk penentuan morfologi ini telah banyak dilakukan. Tujuan penelitian ini menentukan status taksonomi duku, kokosan dan langsat pada ketegori infraspesies berdasarkan karakteritik makro dan mikromorfologi. Berbagai variasi duku, langsat dan kokosan dikoleksi dari beberapa pusat pertanaman di Indonesia, meliputi wilayah Sumatera, Jawa dan Kalimantan. Preparasi herbarium tumbuhan meliputi koleksi sampel, persiapan, penempelan dan identifikasi. Analisis morfologi menggunakan metode pengamatan dan deskripsi, pengukuran karakter morfologi dilakukan baik pada organ generatif dan vegetatif meliputi bunga, batang, biji dan buah, dan semua sampel didokumentasikan dengan menggunakan foto. Hasil penelitian ini menunjukkan ditemukan 29 variasi dari duku, kokosan, dan langsat yang terdiri dari 19 duku, 2 kokosan dan 8 langsat dari semua wilayah. Hasil karakterisasi makro dan mikromorfologi menunjukkan duku, kokosan, dan langsat dari berbagai daerah di Indonesia dapat dinyatakan duku, kokosan, dan langsat merupakan marga Lansium, untuk kategori jenis adalah L. domesticum Correa dan pada kategori infraspesies dapat dibagi menjadi dua group yaitu group duku dan group kokosan-langsat.
DAYA ANTIMIKROBA METABOLIT BIOAKTIF JAMUR SHIITAKE (Lentinula edodes (Berk.) Pegler) YANG DIKULTUR PADA TIGA JENIS MEDIUM FERMENTASI Ekowati, Nuraeni; Kasiamdari, Rina Sri; Pusposendjojo, Nursamsi; Soegihardjo, C.J.
Majalah Obat Tradisional Vol 16, No 3 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/tradmedj.8050

Abstract

Jamur shiitake (Lentinula edodes (Berk.) Pegler) merupakan salah satu jamur yang berpotensi sebagai jamur pangan dan bahan obat (edible and medicinal mushroom). Kultivasi pada medium cair dengan proses fermentasi telah dikembangkan selama beberapa tahun terakhir ini. Empat isolat L. edodes (isolat asal Malang, Cianjur, Lembang dan Yogyakarta) dikultur pada tiga jenis medium fermentasi yaitu (KM: Kauffman Medium; GYMT: Glucose, Yeast extract,  Malt extract, Thiamin; YEMR: Yeast extract,  Malt extract,  Rice bran). Metabolit bioaktif yang diekstrak dari biomassa miselium dan filtrat kultur hasil fermentasi diuji menggunakan mikroba patogen Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 35218, Candida albicans ATCC 10231,dan  Trichophyton mentagrophytes. Data diameter zona  hambat dianalisis menggunakan analisis ragam, dan dilanjutkan dengan uji Duncan pada tingkat kesalahan 5 %. Hasil penelitian menunjukkan bahwa senyawa bioaktif dalam filtrat kultur maupun biomassa miselium L.edodes yang diekstrak menggunakan kloroform, etil asetat dan air, mampu menghambat S. aureus, E.coli dan C. albicans tetapi tidak mampu menghambat T.mentagrophytes. Isolat L.edodes asal Lembang dan Yogyakarta yang dikultur pada medium Kauffman memberikan hasil zona hambat terbaik (24,97-31,14 mm). Mikroba uji yang paling sensitif terhadap senyawa bioaktif dari L. edodes adalah C. albicans diikuti oleh E.coli, S. aureus dan T. mentagrophytes. Metabolit bioaktif dari L. edodes berpotensi menghambat mikroba patogen dari kelompok bakteri dan khamir tetapi tidak menghambat jamur.  
Characterization and Pathogenicity of Fusarium oxysporum as the Causal Agent of Fusarium Wilt in Chili (Capsicum annuum L.) FERNIAH, REJEKI SITI; DARYONO, BUDI SETIADI; KASIAMDARI, RINA SRI; PRIYATMOJO, ACHMADI
Microbiology Indonesia Vol 8, No 3 (2014): September 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.8.3.5

Abstract

Fusarium wilt is a serious disease attacking chili plants in Central Java which cause lost of chili productivity. Fusarium wilt is caused by pathogenic fungi Fusarium oxysporum, which is host specific. The objectives of this research were to characterize the pathogenic F. oxysporum as the causal agent of fusarium wilt in chili plants and to observe the virulence of the pathogen. Fungal pathogen was isolated from Tawangmangu as an endemic area of fusarium wilt in Central Java. The fungi was characterized morphologically and identified molecularly by its internal transcribed spacer regions (ITS regions). Pathogenicity test was done to observe the virulence of the pathogen. One pathogenic strain was isolated from Tawangmangu, Karanganyar and was identified  morphologically and molecularly as F. oxysporum.  
DAYA ANTIMIKROBA METABOLIT BIOAKTIF JAMUR SHIITAKE (Lentinula edodes (Berk.) Pegler) YANG DIKULTUR PADA TIGA JENIS MEDIUM FERMENTASI Ekowati, Nuraeni; Kasiamdari, Rina Sri; Pusposendjojo, Nursamsi; Soegihardjo, C.J.
Majalah Obat Tradisional Vol 16, No 3 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/mot-TradMedJ16iss3pp%p

Abstract

Jamur shiitake (Lentinula edodes (Berk.) Pegler) merupakan salah satu jamur yang berpotensi sebagai jamur pangan dan bahan obat (edible and medicinal mushroom). Kultivasi pada medium cair dengan proses fermentasi telah dikembangkan selama beberapa tahun terakhir ini. Empat isolat L. edodes (isolat asal Malang, Cianjur, Lembang dan Yogyakarta) dikultur pada tiga jenis medium fermentasi yaitu (KM: Kauffman Medium; GYMT: Glucose, Yeast extract,  Malt extract, Thiamin; YEMR: Yeast extract,  Malt extract,  Rice bran). Metabolit bioaktif yang diekstrak dari biomassa miselium dan filtrat kultur hasil fermentasi diuji menggunakan mikroba patogen Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 35218, Candida albicans ATCC 10231,dan  Trichophyton mentagrophytes. Data diameter zona  hambat dianalisis menggunakan analisis ragam, dan dilanjutkan dengan uji Duncan pada tingkat kesalahan 5 %. Hasil penelitian menunjukkan bahwa senyawa bioaktif dalam filtrat kultur maupun biomassa miselium L.edodes yang diekstrak menggunakan kloroform, etil asetat dan air, mampu menghambat S. aureus, E.coli dan C. albicans tetapi tidak mampu menghambat T.mentagrophytes. Isolat L.edodes asal Lembang dan Yogyakarta yang dikultur pada medium Kauffman memberikan hasil zona hambat terbaik (24,97-31,14 mm). Mikroba uji yang paling sensitif terhadap senyawa bioaktif dari L. edodes adalah C. albicans diikuti oleh E.coli, S. aureus dan T. mentagrophytes. Metabolit bioaktif dari L. edodes berpotensi menghambat mikroba patogen dari kelompok bakteri dan khamir tetapi tidak menghambat jamur.  
CELL DISTRUPTION MIKROALGA SECARA ENZIMATIS DENGAN SELLULASE Padil, Padil; Syamsiah, Siti; Hidayat, Muslikhin; Kasiamdari, Rina Sri
REAKTOR Volume 15 No.4 Oktober 2015
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/reaktor.15.4.213-217

Abstract

ENZYMATIC CELL DISRUPTION OF MICROALGAE USING CELLULASE. Micro-algae is one source of potential alternative energy of third generation to be developed as bioethanol raw material. The starch content trapped in Micro-algae cell walls causing the need of cell distruption to release and convert starch into simple glucose before the fermentation process. This study aims to open up the cell walls of Micro-algae and to explore the effect of sellulase enzymes from Aspergillus niger as Micro-algae cell distruption strategy as well as the optimization of process parameters, i.e the concentration of enzyme, temperature, pH, and time which produce the highest glucose yield. The results showed that the highest glucose yield was 82.44% (w/w) obtained at an enzyme concentration of 30% (w/w), temperature 45oC, pH of 4.5 at 40 minutes, the amount of Micro-algae as 0.5 g/L. In general, cell distruption method using sellulase enzyme was proven to be a promising option to open the cell walls of Micro-algae and convert cellulose into simple glucose simultaneously in producing bioethanol.   Keywords: bioethanol; cellulose; cell distruption; enzymatic; micro-algae; starch Abstrak Mikroalga merupakan salah satu sumber energi alternatif generasi ketiga yang potensial untuk dikembangkan sebagai bahan baku bioetanol. Kandungan pati yang terperangkap dalam dinding sel mikroalga menyebabkan perlunya cell distruption untuk melepaskan sekaligus mengkonversi pati menjadi glukosa sederhana sebelum proses fermentasi. Penelitian ini bertujuan untuk membuka dinding sel mikroalga sekaligus mengeksplorasi pengaruh enzim sellulase dari aspergillus niger sebagai strategi cell distruption mikroalga serta melakukan optimasi parameter proses yaitu konsentrasi enzim, suhu, pH, dan waktu yang memberikan yield glukosa tertinggi. Hasil penelitian menunjukkan bahwa yield glukosa tertinggi adalah 82,44% (w/w) yang diperoleh pada konsentrasi enzim 30% (w/w), suhu 450C, pH 4,5 pada waktu 40 menit, dengan jumlah mikroalga 0,5 g/L. Secara umum, metode cell distruption dengan menggunakan enzim sellulase terbukti menjadi pilihan yang menjanjikan untuk membuka dinding sel mikroalga sekaligus mengkonversi selulosa menjadi glukosa sederhana dalam memproduksi bioetanol. 
Genetic Relatedness among Duku, Kokosan, and Pisitan in Indonesia Based on Random Amplified Polymorphic DNA Markers Hanum, Laila; Kasiamdari, Rina Sri; ., Santosa; ., Rugayah
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

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Abstract

Genetic relatedness among duku, kokosan, and pisitan from Indonesia were investigated using random amplified polymorphic DNA (RAPD) markers. Eleven primers (OPA-01, OPA-02, OPA-10, OPB-07, OPB-11, OPB-12, OPB-15, OPT-16, OPU-14, OPU-19, and OPU-20) were used for amplification and yielded a total of 174 DNA bands, of which 167 were polymorphic. Primer OPA-10, OPB-11, OPB-12, OPB-15, and OPU-19 produced all of the polymorphic DNA bands. The size of the amplified DNA fragments ranged from 41-1546 bp. The dendrogram separated into two clusters at a genetic similarity coefficient of 0.76. The cluster 1 consisted of subclusters duku and several pisitan (pisitan OKI, pisitan Sleman, pisitan Hatu, pisitan Punggur, and pisitan Tanjung), and cluster 2 consisted of subclusters kokosan and pisitan. In the kokosan subclusters, including duku Drendan. Dendrogram supported the determination of taxonomic status of duku, kokosan, and pisitan as one species, namely Lansium domesticum Corr. and its divided into two groups, namely L. domesticum ’duku group’ and L. domesticum ’pisitan-kokosan group’. Thus, RAPD analysis was useful tool for determining the genetic variation and the genetics relatedness among duku, kokosan, and pisitan in Indonesia.Key words: duku, kokosan, pisitan/langsat, genetic relatedness, RAPD
Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon Daryono, Budi Setiadi; Aristya, Ganies Riza; Kasiamdari, Rina Sri
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

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Abstract

A random amplified polymorphic DNA (RAPD) marker linked to powdery mildew resistance gene (Pm-I) in melon PI 371795 was reported. However, the RAPD marker has problem in scoring. To detect powdery mildew resistance gene (Pm-I) in melon accurately, the RAPD marker was cloned and sequenced to design sequence characterized amplified region (SCAR) markers. SCAPMAR5 marker derived from pUBC411 primer yielded a single DNA band at 1061 bp. Segregation of SCAPMAR5 marker in bulk of F2 plants demonstrated that the marker was co-segregated with RAPD marker from which the SCAR marker was originated. Moreover, results of SCAR analysis in diverse melons showed SCAPMAR5 primers obtained a single 1061 bp linked to Pm-I in resistant melon PI 371795 and PMAR5. On the other hand, SCAPMAR5 failed to detect Pm-I in susceptible melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to Pm-I evaluated by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers.   Key words: Cucumis melo L., Pm-I, RAPD, SCAPMAR5
The Phylogenetic Relationship Among Varieties of Lansium domesticum Correa Based on ITS rDNA Sequences Hanum, Laila; Kasiamdari, Rina Sri; ., Santosa; ., Rugayah
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

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Abstract

Lansium domesticum Corr. with vernacular name in Indonesian duku has been reported containingtherapeutic bioactive compounds, and some of these compounds shown to be potent antitumor, anticancer,antimalaria, antimelanogenesis, antibacteria, and antimutagenic activities. This plant is commonly known asduku, kokosan and langsat by the local community in Indonesia. The morphological appearance of all varieties isnearly the same, and identifi cation of the varieties is very diffi cult for growers. Variation of DNA sequences ofthe ITS (Internal transcribed spacer) region can be used as a molecular character to determine the phylogeneticrelationship of different varieties of L. domesticum. The aims of this study were to determine taxonomy status ofduku, kokosan, and langsat, also phylogenetic relationship among varieties of L. domesticum based on ITS rDNAsequencing. DNA was isolated from leaves of plant and then amplifi ed using F1 and R1 primers. Nucleotidesequences were identifi ed using Sequence Scanner Software Programm version 1.0, nucleotide sequences from18S, ITS1, 5.8S, ITS2 and 26S region, that has been mergered using EditSeq and SegMan in software Suite forSequence Analysis DNASTAR Lasergene DM version 3.0.25. The results of study showed that DNA fragmentsranging in size from 782-810 bp. Different pattern of DNA fragments indicated polymorphism among duku,kokosan, and langsat. Based on the results of the ITS rDNA sequencing and phylogenetic tree analysis. Itwas determined that Lansium and Aglaia are a separated genus with the similarity index value of 0.98. Duku,kokosan and langsat were divided into two cluster, namely cluster kokosan-langsat and cluster duku with thesimilarity index value of 0.996.Keywords : Phylogenetic relationship, ITS region, L. domesticum, duku, kokosan, langsat
KEBERADAAN FUNGI MIKORIZA ARBUSKULA DI KAWASAN TAILING TAMBANG EMAS TIMIKA SEBAGAI UPAYA REHABILITASI LAHAN RAMAH LINGKUNGAN Suharno, Suharno; Sancayaningsih, Retno Peni; Soetarto, Endang Sutariningsih; Kasiamdari, Rina Sri
Jurnal Manusia dan Lingkungan (Journal of People and Environment) Vol 21, No 3 (2014)
Publisher : Pusat Studi Lingkungan Hidup Universitas Gadjah Mada

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Abstract

Fungi mikoriza arbuskula (FMA) berperan penting dalam menunjang rehabilitasi lahan terdegradasi, termasuk lahan tailing. Tujuan penelitian ini adalah untuk mengetahui keberadaan FMA lokal di lahan tailing tambang emas Timika – Papua, Indonesia. Metode yang digunakan adalah survei dengan mengisolasi FMA dari rhizosfer beberapa jenis tumbuhan dominan di kawasan daerah pengendapan pasir sisa tambang. Pengecatan akar untuk melihat infeksi oleh FMA dilakukan dengan trypane blue, sedangkan perhitungan persen infeksinya dilakukan dengan metode slide. Keberadaan spora FMA dilakukan dengan metode wet sieving. Hasil penelitian menunjukkan bahwa terdapat FMA di lahan tailing di kawasan pengendapan Modified Ajkwa Deposition Area (ModADA). Persentase infeksi tertinggi (>50%) diketahui pada jenis tumbuhan Ficus adenosperma (86,7%), Brachiaria sp (73,3%), Amomum sp (66,7%), Bidens pilosa (63,3%), dan Musaenda frondosa (56,7%), sedangkan beberapa jenis lain mempunyai persen infeksi yang lebih rendah. Jumlah spora pada rhizosfer tumbuhan Brachiaria sp., F. adenosperma, dan Amomum sp., merupakan yang tertinggi dibanding dengan tumbuhan lain yakni 17, 13, dan 11 spora per 10 g tanah. 
Sitotoksisitas Ekstrak Aspergillus fumigatus dari Daun Mekai (Albertisia papuana Becc.) terhadap Sel Kanker Payudara T47D dan MCF-7 Maritsa, Hasnaul; Moeljopawiro, Soekarti; Kasiamdari, Rina Sri
BIO-SITE |BIOLOGI Sains Terapan Vol 1, No 01 (2015): Bio-Site
Publisher : BIO-SITE |BIOLOGI Sains Terapan

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Abstract

The previous studies showed that the Albertisia papuana Becc. root have cytotoxicity on breast cancer. The A. papuana root toxicity on breast cancer could not only by plant secondary metabolism, but may be also by secondary metabolism of endophytes. Aspergillus fumigatus is one of endophytes that have anticancer agent. Endophytes can be distributed dynamically in whole of plant organ, one of them are leaves. Therefore the objective of this studies were to know the presence of A. fumigatus in A. papuana leaves, and the cytotoxicity of their secondary metabolism on breast cancer cells. The sample of A. papuana were collected from Botanical Zoo of Bogor, while T47D and MCF-7 cell lines were obtained of Tropical Medicine’s Faculty, UGM. Isolation of endophytes was done by growing leaves extract on water agar 2 % medium. Secondary metabolism was extracted from fermented broth using in ethyl acetat and n-butanol. The cytotoxicity was perform by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The result showed that A. fumigatus assosiated with A. papuana leaves. Ethyl acetat extract from fermented A. fumigatus both on T47D and MCF-7 cell lines had lower (IC50. 50, 444 µg/ml and 59 µg/ml) than n-butanol (IC50. 103, 398 µg/ml and 127,188 µg/ml. It could be said that A. fumigatus from A. papuana leaves could induce cytotoxicity on T47D and MCF-7 breast cancer cells.   Keywords: cancer, Aspergillus fumigatus, secondary metabolism, Albertisia papuana Becc., cytotoxiciy