L.B.S. Kardono
Research Center for Chemistry, Indonesian Institute of Sciences Puspiptek Area, Serpong 15314 and Pharmacy Graduate School, University of Pancasila, Jagakarsa, Jakarta 126410, Indonesia

Published : 4 Documents

Found 4 Documents

Synthesis of 125I Labeled Estradiol-17β-Hemisuccinate and Its Binding Study to Estrogen Receptors Using Scintillation Proximity Assay Method Susilo, Y.; Mondrida, G.; Setiyowati, S.; Sutari, Sutari; Triningsih, Triningsih; Lestari, W.; Widayati, P.; Ardiyatno, C.N.; Ariyanto, A.; Darwati, S.; Kardono, L.B.S.; Yanuar, A.
Atom Indonesia Vol 38, No 3 (2012): December 2012
Publisher : PPIKSN-BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1276.418 KB) | DOI: 10.17146/aij.2012.175


Research was carried out to obtain a selective ligand which strongly bind to estrogen receptors through determination of binding affinity of estradiol-17β-hemisuccinate. Selectivity of these compounds for estrogen receptor was studied using Scintillation Proximity Assay (SPA) method. Primary reagents required in the SPA method including radioligand and receptor, the former was obtained by labeling of estradiol-17β-hemisuccinate with 125I, while MCF7 was used as the receptor. The labeling process was performed by indirect method via two-stage reaction. In this procedure, first step was activation of estradiol-17β-hemisuccinate using isobutylchloroformate and tributylamine as a catalist, while labeling of histamine with 125I was carried out using chloramin-T method to produce 125I-histamine. The second stage was conjugation of activated estradiol-17β-hemisuccinate with 125I-histamine. The product of estradiol-17β-hemisuccinate labeled 125I was extracted using toluene. Furtherly, the organic layer was purified by TLC system. Characterization of estradiol-17β-hemisuccinate labeled 125I from this solvent extraction was carried out by determining its radiochemical purity and the result was obtained using paper electrophoresis and TLC were 79.8% and 84.4% respectively. Radiochemical purity could be increased when purification step was repeated using TLC system, the result showed up to 97.8%. Determination of binding affinity by the SPA method was carried out using MCF7 cell lines which express estrogen receptors showed the value of Kd at 7.192 x 10-3 nM and maximum binding at 336.1 nM. This low value of Kd indicated that binding affinity of estradiol-17β-hemisuccinate was high or strongly binds to estrogen receptor.Received: 04 December 2012; Revised: 19 December 2012; Accepted: 21 December 2012
Isolation of Bioactive Compounds from Aspergillus terreus LS07 Dewi, Rizna Triana; Tachibana, Sanro; Dewi, Puspa; Kardono, L.B.S.; Ilyas, Muhammad
Indonesian Journal of Chemistry Vol 14, No 3 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.866 KB) | DOI: 10.22146/ijc.21243


This study aims to search for the active components from Aspergillus terreus LS07 which isolated from an Indonesian soil. Bioassay-guided fractionations of the ethyl acetate (EtOAc) extract against α-glucosidase and DPPH free radical to give four isolated compounds: oleic acid (1), ergosterol (2), butyrolactone I (3), and butyrolactone II (4). The structures of these metabolites were assigned on the basis of detailed spectroscopic analysis. Oleic acid (1) was showed significant activity toward α-glucosidase with IC50 value of 8.54 μM, but not for antioxidant. Butyrolactone I (3) and II (4) were showed significant activities against the α-glucosidase with their IC50 values at 52.17 and 96.01 μM, and those against DPPH free radicals at 51.39 and 17.64 μM, respectively. On the other hand, ergosterol (2) did not show any activities.
Teknologi Indonesia Vol 33, No 1 (2010)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v33i1.17


Oncom is a product prepared by fermenting cooked peanut presscake as substrate mixed with tapioca fl our using Mucor sp as inoculum. The aim of this research is to determine the chemical composition and nutritional contents of black oncom using lupine (Lupinus angustifolius) and pigeon pea (Cajanus cajan) as substrate instead peanuts ( Arachis hypogaea ) to evaluate its potential as a new source of protein. The moisture content, pH, texture, solidness, hardness, mold growth and colour of black oncom fermented product were observed. Various tapioca fl our concentration in amount of 5%, 20% and 35% respectively were mixed on peanuts, lupine and pigeon pea as substrates. The fermentation process was conducted for 48 hours at 30oC.The result revealed that the addition 20% of tapioca to substrate showed the best performance of black oncom product and had good sensory characteristics such as texture, taste, aroma and colour based on the sensory evaluation test.
Antioxidant Compound from The Rhizomes of Kaempferia Angustifolia Collected from Purworejo, Central Java Secondary Forest Kardono, L.B.S.; Minarti, Minarti; Sutaryo, B.; Kawanishi, K.
Publisher : Fakultas Farmasi Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (972.071 KB)


Kaempferia angustifolia Rosc. (Zingiberaceae) dan K. rotunda L. di Jawa dikenal sebugai Kunci pepet dan Kunir putih. Kedua macam tumbuhan tersebut biasanya tumbuh di daerah hutan sekunder di Jawa. Rhizoma dari K. angustifolia berbentuk serbuk kering atau tunbuhan segar biasa dijual di pasar tradisional di Indonesia, dan dikenal sebagai pencegah dan pengobatan kanker secara tradisional. Ekstrak khloroform dari rhyzoma K. Angustifolia menunjukkan efek antioksidan (scavenging) yang signifikan terhadap radikal bebas 1,7-difenil2 pikril hidrasil (DPPH). (IC50 = 370 ug/mL). Dua macam senyawa dari ekstrak khloroforin telah diisolasi dan diidentifikasi. Senyawa 1, suatu khalkon yang telah dikenal, yaitu, 2'hidroksi-4', 4', 6'-trimetoksi khalkon adalah senyawa yang aktif (IC5, = 97 ug/mL). Senyuwu 2, suatu sikloheksan epoksid yang telah dikenal, (+)-krotepoksida, tidak aktif (IC;, = 690 ugi mL) Struktur senyawa 1 dan 2 diidentifikasi berdasarkan data spektroskopik, MS dan 2DNMR (HMQC dan HMBC) dan dibandingkan dengan data yang telah dipublikasikan.