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PENAPISAN KHAMIR SELULOLITIK CRYPTOCOCCUS SP. YANG DIISOLASI DARI TANAH KEBUN BIOLOGI WAMENA, JAYA WIJAYA, PROPINSI PAPUA Kanti, Atit
Jurnal Biologi Vol XI, No 1
Publisher : Jurnal Biologi

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Abstract

Cryptococcus sp. was isolated from Kebun Biologi Wamena, Papua. The isolate was able to grow in media with carboxymethyl cellulose as a sole carbon source implying that isolate produced 1-3 ? endo-glucanase. To study the effect of glucose and osmotic pressure, 0.1 % glucose and 0.1 % NaCl were amended into the medium containing CMC. Glucose significantly affected cellulolytic activity and biomass synthesis. At the beginning of cell cultivation glucose augmentation appear to slightly inhibit enzyme activity. Sodium chloride also significantly affected cellulolytic activity. Profile of pH varied dependent on cultivation media. Maximum growth of biomass was achieved after glucose addition, indicating that glucose stimulated cell growth.
Ecological and Taxonomical Perspective of Yeasts in Indonesia SJAMSURIDZAL, WELLYZAR; OETARI, ARIYANTI; KANTI, ATIT; SARASWATI, RASTI; NAKASHIMA, CHIHARU; WIDYASTUTI, YANTYATI; KATSUHIKO, ANDO
Microbiology Indonesia Vol 4, No 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

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Abstract

In the course of ecological and taxonomical study of yeasts in Indonesia, a total of 2147 yeast isolates from 315 samples in the year 2003, 2005, 2006, and 2008 had been obtained from eight locations e.g. Liwa (Sumatera), Cibinong (Java), Cibodas (Java), Kutai (Kalimantan), Enrekang (Sulawesi), Pucak (Sulawesi), Gili and Kuta (Lombok), and Kupang (Timor).  Leaves, flowers, litters, soils, epiphytic soils, insects and insect´s nests were collected for yeasts isolation.  Our molecular identification based on D1/D2 region of nuclear large-subunit rDNA and the internal transcribed spacer (ITS) regions sequence data on 525 representative isolates revealed that 306 isolates belong to 48 described species (18 genera) and 209 strains belong to 19 undescribed species (19 genera), and 10 isolates were discarded because of contamination.  Based on their substrates, litter had the highest yeasts genera (19) followed by soils (18), flowers (10), leaves (6), epiphytic soils (4), and insects and insect´s nests (4).  Genera found on soils were also common on litters.  Yeasts genera found on flowers and epiphytic soils were common on leaves and litters.  The genera Aureobasidium, Cryptococcus, Pseudozyma, Rhodotorula and Sporidiobolus were found in all substrates.  Based on their locations, Kutai had the highest number of genera (15) followed by Cibodas (10), Cibinong (10), Enrekang (10), Kupang (10), Pucak (9), Liwa (7), and Lombok (7).  The genus Cryptococcus was found in all locations.  Our study shed a light to detection of many new taxa of yeasts, 41% of yeasts found in this study represented novel taxa.
KESIAGAAN PAKAN PADA TERNAK SAPI SKALA KECIL SEBAGAI STRATEGI ADAPTASI TERHADAP PERUBAHAN IKLIM MELALUI PEMANFAATAN BIODIVERSITAS FLORA LOKAL Rahmansyah, Maman; Sugiharto, Arwan; Kanti, Atit; Sudiana, I Made
Buletin Peternakan Vol 37, No 2 (2013): BULETIN PETERNAKAN VOL. 37 (2) JUNI 2013
Publisher : Fakultas Peternakan UGM

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Abstract

The presence of local floral resources as forage is essential, especially for the farm survival in anticipaty the impact of the global climate change. The availability of forage in the dry land ecosystems depends on water supply. However, the climate change has caused the change on rainfall pattern including the initiation of the dry season. The problem should be anticipated by the local farmers’ readiness in providing sufficient feed and their ability in performing the excellent management during all seasons. In some areas of East Bali and Nusa Tenggara (West andEast), dry climate crisis 2004 led to feed scarcity. Therefore, this study was conducted in order to identify the potential forage resources in the arid land. The discussion is focused on the results of survey and secondary data collection. Based on the results, there were 22 plants that could be used as forage which were abundant during rainy season, whereas 15 others were able to be forage and silage during the transitional season and along the dry season. The results also showed that the introduction of silage fermentation technique was required as the way to preserve feed for the supply during the dry season. In this study the role of polymer compounds hydrolyzing microbes and the fermentation process by the GRAS (Generally Recognized as Safe) microbes preceeded the role of Lactobacillus plantarum were observed. The results of this study may become a reference in anticipating the global climate change impacts in dry land areas by the application of functional microbial technology in small local farms’ feed management activities.(Key words: Local floral resources, Dry land climate, Fermented feed, Lactobacillus plantarum)
Lipid Accumulation by Flavodon flavus ATH using Palm Oil Mill Effluent as Substrate Sudiana, I Made; Kanti, Atit; Helbert, Helbert; Octaviana, Senlie; Suprapedi, Suprapedi
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol 21, No 2 (2014)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2014.21.2.388

Abstract

Large amount of palm mill effluent is generated annually. The waste would be potential for production of single cell oils (SCOs). The objective of study was to evaluate the lipid accumulation by fungi using palm mill effluent as substrate. To obtain most potential strains for lipid accumulation, seven filamentous fungi isolated from various biomes were evaluated for their ability to produce endoglucanase, and its lipid accumulation. Fungal hypae grown on palm oil mill effluent accumulated lipid of 34,3-87,5 of their dry cell mass. The profile of transesterified SCOs revealed a high content of saturated and monounsaturated fatty acids i.e., palmitic (C16:0), stearic (C18:0) and oleic (C18:1) acids similar to conventional vegetable oils used for biodiesel production. The strain was able to use organic substrates in POME implies that they are promising strain for biofuel feed stock as well as for meeting effluent quality for wastewater discharge.
DIVERSITAS DAN PROFIL METABOLIT SEKUNDER JAMUR ENDOFIT YANG DIISOLASI DARI TUMBUHAN GAMBIR (Uncaria gambler) SERTA AKTIVITAS BIOLOGISNYA SEBAGAI ANTIBAKTERI Jamal, Yuliasri; Ilyas, Muhamad; Kanti, Atit; Agusta, Andria
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (853.072 KB) | DOI: 10.14203/beritabiologi.v9i2.2023

Abstract

Five kinds of endophytic filamentous fungi i.e. Pestalotiopsis sp. GNC, Fusarium sp. GNC-A, Fusahum sp. GNC-B, Pestalotiopsis sp. GUC and Fusarium sp. GUC were isolated from surface sterilized of steems of gambir nasi and gambir udang (Uncaria gambier Roxb.) collected from Rengat, Riau Province,Indonesia. Identification of the endophytic fungi were done through morphologically observations and chemotaxonomically approach. In an antibacterial assays, the ethyl acetate extract of the Pestalotiopsis sp.GNC cultures showed their activity against pathogenic bacteria, Eschericia coli, Staphylococcus aureus and Bacillus sublilis.Beside the above three pathogenic bacteria, the ethyl acetate extract of Fusarium sp. GNC-B culture was also active against
KESIAGAAN PAKAN PADA TERNAK SAPI SKALA KECIL SEBAGAI STRATEGI ADAPTASI TERHADAP PERUBAHAN IKLIM MELALUI PEMANFAATAN BIODIVERSITAS FLORA LOKAL Rahmansyah, Maman; Sugiharto, Arwan; Kanti, Atit; Sudiana, I Made
Buletin Peternakan Vol 37, No 2 (2013): BULETIN PETERNAKAN VOL. 37 (2) JUNI 2013
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.21059/buletinpeternak.v37i2.2427

Abstract

The presence of local floral resources as forage is essential, especially for the farm survival in anticipaty the impact of the global climate change. The availability of forage in the dry land ecosystems depends on water supply. However, the climate change has caused the change on rainfall pattern including the initiation of the dry season. The problem should be anticipated by the local farmers’ readiness in providing sufficient feed and their ability in performing the excellent management during all seasons. In some areas of East Bali and Nusa Tenggara (West andEast), dry climate crisis 2004 led to feed scarcity. Therefore, this study was conducted in order to identify the potential forage resources in the arid land. The discussion is focused on the results of survey and secondary data collection. Based on the results, there were 22 plants that could be used as forage which were abundant during rainy season, whereas 15 others were able to be forage and silage during the transitional season and along the dry season. The results also showed that the introduction of silage fermentation technique was required as the way to preserve feed for the supply during the dry season. In this study the role of polymer compounds hydrolyzing microbes and the fermentation process by the GRAS (Generally Recognized as Safe) microbes preceeded the role of Lactobacillus plantarum were observed. The results of this study may become a reference in anticipating the global climate change impacts in dry land areas by the application of functional microbial technology in small local farms’ feed management activities.(Key words: Local floral resources, Dry land climate, Fermented feed, Lactobacillus plantarum)
DIVERSITY OF XYLOSE ASSIMILATING YEAST FROM THE ISLAND OF ENGGANO, SUMATERA, INDONESIA [Keragaman Khamir Pengguna Xilose yang Diisolasi dari Pulau Enggano, Sumatera, Indonesia] Kanti, Atit; Sumerta, I Nyoman
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3160.618 KB) | DOI: 10.14203/beritabiologi.v15i3.2215

Abstract

Naturally occurring yeasts (wild type) are important genetic resources for many industrial interests. Though biodiversity exploration of Enggano has been done since 1944, but there is no report for yeast diversity from this island. Due to its industrial interest, xylose fermenting yeast is target for many scientists. This present study aims to isolate xylose assimilating yeast from island of Enggano. The samples were collected from varying sources included soil, leaf litter, decay wood, fruit and sediment. Xylose enrichment culture technique was performed to enrich xylose fermenting yeast. While for the isolation of yeast several methods which include dilution, ballistopore falling, direct isolation, membrane filtration were done. Of 200 isolates, 76 strains (38 %) were xylose assimilating yeast which mostly belong to Candida which include C. insectorum, C. tropicalis, C. boidinii, C. pseudolambica, C. yuanshanica, C. silvae, Cyberlindnera saturnus, Williopsis saturnus and Sporobolomyces poonsookiae. One strain Candida sp. that was isolated from soil could be assigned as a candidate of novel species on base on its morphological and biochemical characteristics along with analyses of gene sequence from D1/D2 domain. This study confirms that yeast survey is very important to obtain new genetic resources for industrial interest as well as for taxonomic study.
Carboxymethyl Cellulose Hydrolyzing Yeast Isolated from South East Sulawesi, Indonesia Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1163.197 KB) | DOI: 10.14203/jbi.v11i2.2203

Abstract

ABSTRACTThe objective of study was to isolate, identify and characterize the CMC-ase producing yeast from South East Sulawesi, Indonesia.  We isolated 142 strains and obtain 53 strains (37.32%) were CMC-ase producer consist of 26 species residing within 10 genera.  Candida was the most diverse genus consisting of 15 species. It is important to note that several strains residing within this genus could be candidate for new taxa, among others Candida aff. cylindracea PL2W1, Candida aff. insectorum PL3W6, Candida aff. friedrichii  MKL7W3, Candida aff. lessepsii, Candida aff. tenuis.  Five new candidates for novel species of cellulolytic yeast close to Yamadazyma mexicana: were Yamadazyma aff. mexicana (5 strains). Pichia, Pseudozyma, Sporodiobolus, and Sporobolomyces were other cellulolytic yeasts found in South East Sulawesi.  It is obvious, that leaf litter was a good source for cellulolytic yeasts. This CMC-ase producing yeasts dominate this biome, and production of extracellular cellulase is critical strategy for such yeast to survive in cellulose rich ecosystem such as leaf-litter.  This finding would suggest that yeasts play key role on hydrolyzes of cellulose and important resources for sustainable energy research. Keywords: cellulolytic yeasts, secondary forest, South East Sulawesi, leaf-litter 
Aktivitas Enzim Selulase dari Khamir Candida sp. dan Debaryomyces sp. yang Diisolasi dari Lahan gambut Taman Nasional Bukit Duabelas Jambi Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 4, No 1 (2006): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (645.864 KB) | DOI: 10.14203/jbi.v4i1.3273

Abstract

ABSTRACTCellulalytic Activity of Candida sp and Debaryomyces sp Isolated from Peat Soil ofBukit Duabelas Natural Park, Jambi. Candida sp. and Debaryomyces sp. YeastDegrading cellulose Isolated from Peat Soil in Bukit Duabelas National Park, Jambi. Theobjective of study was to investigate the characteristic of yeast that are able to solubilizecellulose. The yeast was isolated from soil of Tarnan Nasional Bukit Duabelas Jambi.Out of 2 1 ioslates tested 2 isolates Candida sp. (Isolate J I) and Debaryomyces sp. (IsolateJ2) were able to solubilize Carboxymethyl cellulose. Other isolated soil yeasts wereRhodotor& sp, and C~ptococcusp . Medium acidity during cell cultivation variedbetween 5.8 to 7.2. The CMC-ase activity was 5.9 unit and 5.4 unit for isolate J1 andisolate 52 respectively. The Km of isolate J1 and isolate 52 were 7.7 x 10" and 8.4 x 1 0-2(% b/v) respectively. Vmaks of isolate J1 and J2 was 8.28 xlW3 dan 30.66 x lo-) pgglukosa/ml enzyme /minute, respectively.Key words: Cellulolytic Yeasts, Candida sp., Debaryomyces sp., Enzymes Activity,Taman Nasional Bukit Duabelas
Keragaman Jenis Khamir Penghasil Etanol yang Diisolasi dari Makanan Fermentasi di Kepulauan Riau Sumerta, I Nyoman; Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (901.807 KB) | DOI: 10.14203/jbi.v13i1.3096

Abstract

ABSTRACTInformation on genetic diversity of fermentative yeast which produce ethanol is very crucial in developing biofuel production in Indonesia. Research on ethanol producing yeasts is interest of many scientist. The objective of study was to reveal yeast diversity in Indonesian fermented foods that able to produce ethanol. The sample of fermented foods were collected in the traditional market in Karimun Besar Island, Kepulauan Riau. Yeast isolation was performed using serial dilution with direct plating and enrichment culture with glucose as carbon source. Fifteen of isolates were isolated and identified by amplification of D1/D2 region LSU 26S rDNA. Its ethanol production characteristic was analyzed base on fermentation activity and measurement with gas chromatography for ethanol content. The result revealed that 8 yeast species were found belong to Ascomycetous and grouped into 5 clades which are able to produce ethanol. The highest ethanol production was obtained by Saccharomyces cerevisiae Y15Kr107 (3.53%) followed by Torulaspora delbrueckii Y15Kr104 (1.63%), Saccharomyces cerevisiae Y15Kr093 (1.58%), Candida glabrata Y15Kr110 (1.4%), Torulaspora delbrueckii Y15Kr103 (1.29%), Candida glabrata Y15Kr108 (1%), Torulaspora globosa Y15Kr094 (0.92%), Kodamaea ohmeri Y15Kr096 (0.61%), and Pichia kudriavsevii Y15Kr106 (0.31%) Y15Kr105 (0.21%) Y15Kr109 (0.16%). Other yeasts strains did not produce ethanol but may play different role in fermentation process.Key words: yeast, fermented food, ethanol, Kepulauan Riau