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Amplified Fragment Length Polymorphism Diversity of Cultivated White Oyster Mushroom Pleurotus ostreatus Jusuf, Muhammad
HAYATI Journal of Biosciences Vol 17, No 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.17.1.21

Abstract

Amplified fragment length Polymorphism (AFLP) analysis was performed to study the genetic diversity of fifteen isolates of white oyster mushroom (Pleurotus ostreatus) originated from different places in Indonesia (Java, Sumatra, Bali, and Kalimantan) and Thailand. The majority of studied isolates originated from commercial mushroom, and some of them were collected from nature. AFLP analysis revealed 202 loci of positive DNA bands. Heterozygosity value showed polymorphisms of the fifteen studied isolates. Correlation and cluster analyses of the isolates showed that isolate from Thailand (BNK isolate), isolate from Bogor (AMD isolate), and isolate from Purwokerto (USX isolate) were distinctly different from the other isolates indicated by positive bands with DNA size greater than 650 bp. Isolate from Kalimantan (BJM isolate) was distinctly different from Java island isolates. Generally there was no correlation of AFLP variability and geographic distribution of commercial strain isolates.
TOLERANSI TANAMAN KEDELAI TERHADAP CEKAMAN AIR : AKUMULASI PROLIN DAN ASAM ABSISIK DAN HUBUNGANNYA DENGAN POTENSIAL OSMOTIK DAUN DAN PENYESUAIAN OSMOTIK Sopandie, Didy; Hamim, ,; Jusuf, Muhammad; Heryani, Nani
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 24, No 1 (1996): Buletin Agronomi
Publisher : Bogor Agricultural University

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Abstract

In this experiment. the changes on leaf osmotic potential and accumulation of proline and abscisic acid were identified from drought-tolerant and drought - sensitive soybean genotypes. Three drought - tolerant (Mlg 2805, Mlg 2984 and Mlg 2999) and two sensitive soybean genotypes (Mlg 2510 and Mlg 3541) were subjected to drought condition created by regulating water supply in greenhouse.  The results revealed that exposing plants to drought stress brought about a decrease of leaf osmotic potential. The decrease of which was greater in drought-tolerant genotypes (6.91 to 10.11 bars) than in sensitive genotypes (0.55 to 0.69 bars). The decreasing of leaf osmotic potential was followed with increasing praline accumulation, especialy for Mlg 2805. Only Mlg 2805 showed the significant ABA accumulation when the plants were subjected to drought stress. It is suggested that the drought tolerance was associated with the reduction of leaf osmotic potential (osmotic adjusment) in which proline might play an important role. The role of ABA could not be clarified since there had been a great variability in ABA content of all tolerant genotypes.
Hubunagn kemampuan pergantian inang dengan plastisitas genetika pada cendawan blas padi (Pyricularia grisea) Listiyowati, Sri; Widyastuti, Utut; Rahayu, Gayuh; Hartana, Alex; Jusuf, Muhammad
Jurnal Ilmu Pertanian Indonesia Vol 14, No 2 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The Digitaria ciliaris, wild grass grown around rice field, was a host for Pyricularia grisea (Cooke) Sacc., the fungi caused blast disease of rice. This fungi have a specific mechanism to regenerate new genetic variation in its life cycle. The aim of this research is to study the relation between the ability of the fungi to infect different species of host with its genetic plasticity. It was used three SCAR molecular markers Cutl, Pwl 1 and Erg2. P.grisea isolates (Dc4J1) originated from D. ciliaris at Jasinga-Bogor were able to infect rice cultivars Kencana Baliand Cisokan. The original Dc4Jl, from D. ciliaris, and the Dc4Jl that were reisolated from the infected rice cultivars (reisolates-1) had the same ability to infect Kencana Bali and Cisokan. Molecular technique showed that there was a different molecular marker genotype between the original Dc4J1, from D. ciliaris, and the Dc4Jl reisolated from infected rice cultivars. The original Dc4J1 owned Cutl but did not Pwl2 in contrary the reisolatesDc4J1 from rice cultivars (reisolates-1) had Pwl2 but did not Cutl. The Erg2 presented in both the original and the reisolated Dc4Jl. These results indicated that there were a change of genotype of P. grisea at the same time with the change of host species. The Dc4Jl isolates originated from Kencana Bali and Cisokan (reisolates-2) that were infected by reisolate-1, had the same genotype with the reisolates-1. Keywords: Digitaria ciliaris, Pyricularia grisea, genetic plasticity.
Computation Technique to Estimate Recombination Value, With an Example Loci of MDH, Esterase and Hypocotyl Colour of Setaria italica Jusuf, Muhammad
Forum Pasca Sarjana Vol 12, No 1 (1989): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Abstract

Coefficient of recombination between two loci can be estimated from it´s maximum likelihood function. Newton approximation method that was combined with computer programation can be used on that estimation. Application of this method to data of Setaria italica F2 population, showed recombination coefficient between Est-3 and MDH-I loci is 33.25 + 2.92% and between hypocotyl color and EST-2 loci is 10.21 + 3.25%.
TOLERANSI TANAMAN KEDELAI TERHADAP CEKAMAN AIR: UJI LAPANG BEBERAPA GENOTIPE TOLERAN Sopandie, Didy; Hamin, ,; Jusuf, Muhammad; Supijatno, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 25, No 2 (1997): Buletin Agronomi
Publisher : Bogor Agricultural University

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Abstract

The purpose of this field verification was to determine the stability of yield of several drought-tolerant soybean genotypes selected from green house evaluation. The plants were planted at Muneng, Probolinggo in dry season (June-September 1995) with and without irrigation. From 5 tolerant genotypes, Mlg 2999 and Mlg 3474 gave a good stability of tolerance as evidence by less significance of growth and grain yield reduction. The tolerant genotypes of Mlg 2805 and Mlg 2984 suffered from tremendous leaves and pods abcission, leading to low yield.
Analisis Generasi F2 dan Seleksi Pertama dari Persilangan Kedelai antara Kultivar Slamet dan Wase Suharsono, ,; Jusuf, Muhammad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 37, No 1 (2009): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The research had an objective to analyse the population of F2 and first selection (S1) generations of the cross between soybean cultivar Slamet and Wase. The ultimate goal of this research is to obtain the elite cultivars of soybean having high yield, big seeds, and tolerant to acid and aluminum stresses. The genetic variance and heritability in the broad sense of all characters observed of F2 population were very high. The seed productivity of F2 population was higher than that of Slamet and Wase cultivars. The size of seeds of F2 population was bigger than that of Slamet and comparable to that of Wase. The heritability in the broad sense of all characters of F2 population was very high because the maximum segregation occurs in F2 and the two parents had a very different genetic back ground. By using 7.5% selection intensity based on productivity, we got selected F2 population having productivity two times than that of Slamet. Seeds of this selected F2 population were bigger than that of F2 population and cultivar Slamet. The S1 population had seed productivity higher than Slamet and Wase cultivars. The heritability in the broad sense of all characters of S1 population was smaller than that of F2 population caused by the selection. The selection by 4.8% intensity resulted the selected S1 population having productivity three times than that of cultivar Slamet and big seeds. The selected S1 population had a phenotype variance of productivity smaller than S1 population. Therefore, the selected S1 population is very potential to be developed as elite soybean cultivars.   Key words: soybean, genetic variance, heritability, selection, F2 population
Keragaan dan Keragaman Genetik Sifat-sifat Kuantitatif Kedelai (Glycine max L. Merrill) pada Generasi Seleksi F6 Persilangan Varietas Slamet x Nakhonsawan1 Jambormias, Edizon; Sutjahjo, Surjono H.; Jusuf, Muhammad; Suharsono, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 35, No 3 (2007): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

A field experiment to obtain new improved soybean varieties with higher yield and seed size was conducted by crossing Slamet Variety (high yield, small seed size) with Nakhonsawan (large seed size) in order to produce F6 selected generation. The process of selection and evaluation was conducted during a 3.5 month period, from August to December 2003 in KP IPB Sindang Barang Bogor. The pedigree selection method was used in the experiments. Data was analyzed based on information of set of total data, relatives and individually. The results showed that low performance for all traits compared to the Slamet variety except seed size and seed production traits, and higher than Nakhonsawan variety except seed size. However, genetic variances and heritabilities were high for all traits except number of branch. On the other hand, distribution of genetic variances and heritabilities on all levels of relatives were small except for the within-family F6 generations. This indicated that there was an effect of over-dominance gene action. Conclusion of analysis showed existence of two families with high seed production and seed size if compared to Slamet variety.   Key words: Soybean, selection, performance, genetic variability, and heritabilities.
Diversity of SCAR Markers of Pyricularia grisea Isolated from Digitaria ciliaris Following Cross Infection to Rice LISTIYOWATI, SRI; WIDYASTUTI, UTUT; RAHAYU, GAYUH; HARTANA, ALEX; JUSUF, MUHAMMAD
Microbiology Indonesia Vol 5, No 1 (2011): March 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.5.1.1

Abstract

Cross infection of Pyricularia grisea from grass to rice and vice versa has been reported, but genetic changes are not known yet.  This research  aimed at estimating the possibility of the genotype alteration in P. grisea dc4 isolated from Digitaria ciliaris, following cross infection to either rice cv. Kencana bali, Cisokan, and IR64 or Panicum repens, Cynodon dactylon, Digitaria sp., and Ottochloa nodosa. The genotypes were analyzed by employing three SCAR markers, Cut1; PWL2; and Erg2.  The results indicated that the dc4 was only able to infect Kencana bali, Cisokan, and P. repens.  The dc4 had only two out of three SCAR markers, Cut1 and Erg2.  Host shift was followed by genotype alteration in two loci of SCAR.  Isolates derived from lesions on Kencana bali (dc4-kb) and Cisokan (dc4-c) of the dc4 infection, both lost their Cut1 and gained PWL2. On the contrary, there was no genotype alteration from dc4 to isolate derived from P. repens of dc4 infection (dc4-pr).  Neither the isolate dc4-kb  that was cross-inoculated to Cisokan nor the dc4-c that was cross-inoculated to Kencana bali showed SCAR marker change.  In comparison,  race 173 isolate and those derived from Kencana bali and Cisokan did not show genotype alteration. All had two out of three SCAR markers, PWL2 and Erg2.  The isolate 173 was adapted to rice. This indicated that genotype diversity of the dc4 might arise following host shift from grass to rice.Pyricularia grisea merupakan cendawan blas yang telah diketahui memiliki kisaran inang luas selain pada padi. Infeksi silang cendawan blas pada rumput ke padi dan sebaliknya telah dilaporkan, tetapi perubahan genetiknya belum dilaporkan. Tujuan penelitian ini menganalisis kemampuan infeksi silang dan perubahan genotipe P. grisea dc4 asal Digitaria ciliaris dalam perpindahannya ke padi cv. Kencana bali, Cisokan, dan IR64 atau rumput Panicum repens, Cynodon dactylon, Digitaria sp. dan Ottochloa nodosa. Genotipe P. grisea dianalisis melalui tiga marka SCAR, yaitu Cut1; PWL2; dan Erg2. Isolat dc4 memiliki 2 marka SCAR, yaitu Cut1 dan Erg2; tidak memiliki PWL2. Isolat dc4 hanya mampu menginfeksi silang Kencana bali, Cisokan, dan P. repens. Turunan isolat dc4 sebagai hasil infeksi silang ke Kencana bali (dc4-kb) dan Cisokan (dc4-c) menunjukkan perubahan genotipenya, yaitu Cut1 tidak teramplifikasi pada keduanya; PWL2 teramplifikasi;, serta Erg2 tetap teramplifikasi. Sebaliknya, turunan isolat dc4 sebagai hasil infeksi silang ke P. repens (dc4-pr) tidak mengalami perubahan genotipe. Turunan isolat dc4-kb sebagai hasil infeksi silang ke Cisokan, maupun turunan isolat dc4-c dari Kencana bali, juga tidak menunjukkan perubahan genotipe, yaitu tetap menunjukkan keberadaan PWL2 dan Erg2. Sebagai pembanding digunakan isolat ras 173 yang diisolasi dari padi.  Genotipe isolat tersebut maupun turunannya, sebagai hasil infeksi silang ke Kencana bali dan Cisokan, tidak menunjukkan perubahan. Perubahan genotipe dc4 terjadi mengikuti pergantian inang dari rumput ke padi.
Screening of Genomic Library of Soybean Cultivar Lumut by Using Peroxidase Gene from Arabidopsis thaliana as Probe Suharsono, Suharsono; Juliyanto, Teguh; Jusuf, Muhammad
Jurnal ILMU DASAR Vol 11, No 1 (2010)
Publisher : Fakultas MIPA Universitas Jember

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Abstract

Screening to genomic library of soybean cv. Lumut by using gene encoding for peroxidase (per) from A. thaliana as a probe has an objective isolate the whole gene of per from soybean. The probe was labeled by non-radioisotope alkalin phosphatase. Screening was done by two steps. The first, screening was done to 105 recombinant lambda phages containing genome of soybean cv. Lumut. After southern hybridization, positive signal of plaques were isolated and screened for the second time. After second screening, some recombinant lambda phages containing putatively per genes were isolated. Excision from recombinant lambda phages into recombinant plasmid was successfully done in Escherichia coli strain BM25.8. The plasmid DNAs were isolated from E. coli strain BM25.8 and introduced into E. coli strain DH5α for multiplication. Plasmid DNAs were digested by EcoRI and transferred onto nylon membrane hybond N+. Southern hybridization analysis showed that one clone, L10/R/3/4, contain per gene in the 7.7 kb EcoRI fragment. This fragment is inserted into pSportI.Keywords: Genomic library, soybean, peroxidase, screening
DISTRIBUTION OF Hoya multiflora Blume AT GUNUNG GEDE PANGRANGO NATIONAL PARK, INDONESIA Rahayu, Sri; Kusmana, Cecep; Abdulhadi, Rochadi; Jusuf, Muhammad; Suharsono, Suharsono
Indonesian Journal of Forestry Research Vol 7, No 1 (2010): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

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Abstract

Hoya multiflora is one of the valuable germplasm in Indonesia that has been utilized as ornamental and medicinal plant. This epiphytic plant faces problems in decreasing habitat. As a means for developing a habitat framework for describing the distributions and ecological relations of H.multiflora at Gunung Gede Pangrango National Park, Bogor, Indonesia, ecological study of this species was established over the ranges of altitudes and characteristic vegetation structural types (primary and secondary forest, and plantation) present in the Park. Recognizing the fact that such study requires multidisciplinar y data, this paper explores the evidences from both herbarium sheets and field observations. The result of the study showed that the population of this species was only found at the Bodogol Research Station at elevation of 700 - 900 m above sea level (a.s.l.). Thus, the facts contradict with the evidence from the herbarium sheets of the Herbarium Bogoriense which have presumed that this species has a wide variation of altitudinal range from 20 to 1500 m a.s.l. (Indonesia) or 200 - 1400 m a.s.l. ( Java). The Bodogol’s population showed the clumped type of dispersion (Morisita’s Index = 1.35), which indicated such environment that was characterized by patchy resources. Direction and speed of wind coupled with the topography are ecological factors that affect to the distribution of this parachute typed seeds of the H.multiflora.