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Y-Chromosome Microsatellite DNA Variations on Terunyan Bali Mula Society JUNITHA, I KETUT; SUDIRGA, SANG KETUT
HAYATI Journal of Biosciences Vol 14, No 2 (2007): June 2007
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.14.2.59

Abstract

The Balinese is comprised of genealogical patrilineal clans named as soroh. However, remnants of ancient Bali Mula communities have their own local genealogy which differ from the general soroh. The Pasek Trunyan together with Pasek Kayu Selem, Pasek Celagi, and Pasek Kayuan are part of Bali Mula which believed to be descendants of one ancestor, Empu Kamareka. Here we reported genetic variation of the Terunyan society. The microsatellites Y-chromosome markers (DYS19, DYS390, DYS393, and DYS395) were used in this study. There were 12 alleles found and the result showed the low genetic diversity (D = 0.28 + 0.05) within Terunyan society. Nine haplotypes based on allelic combination were found, dominated by major haplotype with frequency of 0.50 which spread out in many genealogical lineage called dadia. The haplotype data showed that Terunyan society derived from many gene sources. Key words: patrilineal, pasek, soroh, dadia, haplotipe
VARIASI GENETIK SUKU BATAK YANG TINGGAL DI KOTA DENPASAR DAN KABUPATEN BADUNG BERDASARKAN TIGA LOKUS MIKROSATELIT DNA AUTOSOM UNADI, YOSSY CAROLINA; NARAYANI, INNA; JUNITHA, I KETUT
Jurnal Biologi Vol XIV, No 2
Publisher : Jurnal Biologi

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Abstract

Study of genetic variation on three microsatellite DNA loci D2S1338, D13S317 and D16S539 from 76 unrelated individuals of Batak ethnic who live in Denpasar city and Badung regency were analyzed by using PCR method (SuperMix Kit, Invitrogen). DNA samples were extracted from blood cell using phenol-chloroform method and ethanol precipitation. The result of this study found 14 alleles on D2S1338 locus, 10 alleles on D13S317 locus and eight alleles on D16S539 locus. The finding showed high genetic diversity on three loci as well as on three sub-ethnic Batak of Batak Toba, Karo and Simalungun which were 0.8637; 0.7314 and 0.7692 respectively.
MORPHOLOGICAL CHARACTERS OF CHILLI PEPPER (Capsicum frutescens L.) AS INFLUENCED BY SODIUM AZIDE AT GENERATIVE STAGE OF M1 GENERATION Saraswati, I Gusti Agung Eka; Pharmawati, Made; Junitha, I Ketut
Jurnal Biologi Vol XVI, No 1
Publisher : Jurnal Biologi

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Abstract

Chili pepper (Capsicum frutescens L.) is one vegetable crop that has high economic value.  There are several problems in cultivation of chili pepper, for examples limited land, bad weather, and pests and diseases, which result in low production. These problems can be solved by developing new cultivar which has superior quality.  New cultivars of chili pepper plants can be obtained through induced mutation using chemical mutagen. In this research sodium azide (NaN3) was used as mutagen in chili pepper in order to obtain genetic variations that are useful in  increasing its  production.  The research was designed in  randomized  block  design. The observations were made on the response of plant morphology at generative stage, including plant height, number of branch, number of leaf, leaf length and leaf width.  The results showed that sodium azide increased plant height, branch number, leaf number and leaf length.  Sodium azide at concentrations of 2 mM and 5 mM were most effective in increasing plant height, number of leaves and number of branches. Keywords: Capsicum frutescens L., morphology, mutation, sodium azide
METODE ANALISIS KUALITATIF DAN KUANTITATIF LDL-C MENGGUNAKAN ELEKTROFORESISAGAROSE DAPAR TAE (TRIS-ASAM ASETAT-EDTA) Setyari, Putu Rosi; Wirasutha, IMA Gelgel; Junitha, I Ketut
Jurnal Farmasi Udayana Vol. 1, No. 1, Tahun 2012
Publisher : Jurnal Farmasi Udayana

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Abstract

In this research had been done the development of analysis qualitative and quantitative methods of LDL-C in human serum using agarose electrophoresis. The methods that had been developments are separation lipoprotein in human serum using agarose electrophoresis with TAE pH 8,6 buffer system and quantification methods for LDL-C using spectrophotometer UV-Vis. The aim of this research is to find out a simple method that can separate lipoproteins of human serum and quantificating LDL-C. The results of this research shows agarose electrophoresis with TAE pH 8,6 buffer system could  separate LDL-C in human serum as good as electrophoresis with Na-Barbital buffer system. However, for its quantification only can be done with spectrophotometer UV-Vis.
PENGARUH MUTAGEN KIMIA SODIUM AZIDA TERHADAP MORFOLOGI TANAMAN CABAI BESAR (Capsicum annuum L.) Yunita Sari, Ni Kadek; Pharmawati, Made; Junitha, I Ketut
Metamorfosa: Journal of Biological Sciences Vol 1 No 1 (2012)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

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Abstract

The aim of this research was to examine the effects of sodium azide at concentration of 1 mM, 2 mM, 3 mM, 4 mM and 5 mM on the morphological characters of Capsicum annuum L. The method used for the observation of morphological characters was direct observation by measuring plant height, leaf length and width as well as counting the number of leaves. The results showed that effect of sodium azide at 3 mM caused an increase in plant height, while concentration of 1 mM, 2 mM, 4 mM dan 5 mM caused a decrease in plant height. Similar trends were found for number of leaf. Leaf length and width tended to increase in sodium azide treatments.
PREDIKSI LAMA KEMATIAN BERDASARKAN KEBERADAAN SERANGGA GENUS LUCILIA (CALLIPHORIDAE) PADA BANGKAI MENCIT (Mus musculus) DI LOKASI HUTAN MANGROVE Laksmita, Ayu Saka; Watiniasih, Ni Luh; Junitha, I Ketut
Jurnal Biologi Vol 17, No 1 (2013): Jurnal Biologi
Publisher : Jurnal Biologi

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Abstract

This study aimed to investigate the presence of insects on mice carcasses in mangrove forest, the chronology of the insect presence and growth phase of the insects on estimating the time of death of the animal for the beneficial of forensic study. Collected insects were identified at The Laboratory of Animal Taxonomy and The Laboratory of Ecology, Department of Biology, Udayana University. The samples were divided into two based on the study areas: dry and watered areas. Five mice carcasses in three different spot were laid on each dry area. The data collected were analyzed descriptively. The differences of insect growths from egg to pupae and also the ex-pupae found on carcasses could be used to estimated the time of death of an animal.
PREDIKSI LAMA KEMATIAN BERDASARKAN KEBERADAAN SERANGGA GENUS LUCILIA (CALLIPHORIDAE) PADA BANGKAI MENCIT (Mus musculus) DI LOKASI HUTAN MANGROVE Laksmita, Ayu Saka; Watiniasih, Ni Luh; Junitha, I Ketut
Jurnal Biologi Vol 17, No 1 (2013): Jurnal Biologi
Publisher : Jurnal Biologi

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Abstract

This study aimed to investigate the presence of insects on mice carcasses in mangrove forest, the chronology of the insect presence and growth phase of the insects on estimating the time of death of the animal for the beneficial of forensic study. Collected insects were identified at The Laboratory of Animal Taxonomy and The Laboratory of Ecology, Department of Biology, Udayana University. The samples were divided into two based on the study areas: dry and watered areas. Five mice carcasses in three different spot were laid on each dry area. The data collected were analyzed descriptively. The differences of insect growths from egg to pupae and also the ex-pupae found on carcasses could be used to estimated the time of death of an animal.
PROFIL STRUKTUR SERAT IBU TANGKAI DAUN ANTARA INDUK DAN ANAKAN KELAPA (Cocos nucifera L “Rangda”) Puspawati, Ni Made; Kriswiyanti, Eniek; Junitha, I Ketut
SIMBIOSIS Journal of Biological Sciences Vol I, No 2, Tahun 2013
Publisher : SIMBIOSIS Journal of Biological Sciences

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Abstract

The research aims to determine the anatomical structure of the leaf stalk fiber Coconut(Cocos nucifera L. "Rangda") on the seedling and parent plant. Sampling was conducted atSanghyang village Melaya district, Regency of Jembrana. Preparations and microscopicobservations made in the laboratory of Plant Development Structure Biology Department,FMIPA Unud. The research was conducted in October 2012 - April 2013. Maceration methodused for preparations sklerenkim fibers and xylem elements. The results showed that theanatomical parent plant and seedling petiole were torque and mostly composed bysclerenchym fibers but in the parent plant more torque than seedling. In the parent plant about81% the sclerenchym cell was torque but in seedling only 13%, with an average number of17 ± 1 torsion of the parent plant and 6 ± 1 in seedling.Keywords: the fiber structure, torque
EKSTRAKSI DNA SPERMA PADA KONDOM YANG DISIMPAN DALAM RENTANG WAKTU BERBEDA Lanang, A.A. Gde; Junitha, I Ketut; Suaskara, I.B. Made
Jurnal Biologi Vol 17, No 2 (2013): Jurnal Biologi
Publisher : Jurnal Biologi

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Abstract

Sperma adalah bahan biologis yang sering digunakan sebagai bukti untuk kasus pemerkosaan. Penelitian ekstraksi DNA dari sperma dilakukan untuk mengetahui apakah DNA dapat diekstraksi dari sperma pada kondom yang tersimpan selama 15, 20, 25, 30, dan 35 hari serta untuk mengetahui keberhasilan amplifikasinya. Sampel sperma dari seorang probandus diteteskan ke dalam kondom 2000 µL, kemudian disimpan selama 15, 20, 25, 30, dan 35 hari. Ekstraksi DNA dilakukan dengan menggunakan metode fenol-klorofom yang sudah dimodifikasi dan amplifikasi DNA dengan menggunakan PCR Mastermix. Hasil penelitian menunjukkan bahwa DNA masih dapat diekstraksi dan diamplifikasi dari sperma dalam kondom yang tersimpan hingga 35 hari.  
PENELITIAN PENDAHULUAN VARIASI GENETIK MASYARAKAT SOROH PANDE BERDASARKAN PENANDA DNA MIKROSATELIT KROMOSOM Y: MASYARAKAT SOROH PANDE DESA ABIANSEMAL, BADUNG Wulandari, Ni Putu Putri; Junitha, I Ketut; Wirasiti, Ni Nyoman
Jurnal Biologi Vol 18, No 1 (2014): Jurnal Biologi
Publisher : Jurnal Biologi

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Abstract

Masyarakat Hindu di Bali dibedakan ke dalam kelompok-kelompok berdasarkan garis keturunan laki-laki yang disebut klan atau di Bali lebih dikenal dengan istilah soroh. Salah satu soroh yang dikenal di Bali adalah soroh Pande. Penelitian ini bertujuan untuk mengetahui variasi genetik masyarakat soroh Pande di Desa Abiansemal, Badung menggunakan empat lokus mikrosatelit kromosom Y, yaitu DYS19, DYS390, DYS393 dan DYS395. Berdasarkan hasil analisis, didapatkan 11 ragam alel dan enam haplotipe yang didominasi oleh haplotipe A (200, 215, 121, 123) dengan frekuensi sebesar 0,65 serta keragaman genetik sebesar 0,32 ± 0,07. Ragam haplotipe yang didapatkan menunjukkan bahwa masyarakat soroh Pande di Desa Abiansemal berasal dari berbagai sumber gen.