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SIFAT PROTEKSI EKSTRAK AIR PANAS TEH {Camellia sinensis (L.) Kuntze} HIJAU PADA KHAMIR Candida tropicalis YANG DIPERLAKUKAN DENGAN PARACETAMOL Julistiono, Heddy
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.535 KB) | DOI: 10.14203/beritabiologi.v10i6.1943

Abstract

In order to develop yeast Candida tropicalis as a model cell for evaluation of substance having anti- or pro-oxidant activity in cell level, the effect of hot water tea {Camellia sinensis (LJ Kuntze) extract on peroxidized lipids, a marker of oxidative stress and cell mortality in the yeast caused by paracetamol has been evaluated. Incubation of yeast cell in the presence of 1.38 % green tea for 2 h decreased cell viability followed with increasing of peroxidized lipids, whereas 0.27 % green tea caused increasing of peroxidized lipids without causing cell death.Yeast cell was not affected by 0.1 % green tea. Incubation of yeast cell with presence of 0.15 % (g/v) paracetamol for 2 h did not cause cell mortality, however content of peroxidized lipids increased significantly. In the presence of higher dose of paracetamol (0.3 %) cell viability remarkably decreased and followed with increasing of peroxidized lipids significantly. Green tea of 0.1 % increased cell viability of cells treated with 0.3 % paracetamol while peroxidised lipids decreased. The data indicated that high dose of paracetamol caused oxidative stress in yeast cells, while green tea with lower concentration might protect paracetamol toxicity due to its antioxidant property. Although the antioxidant property, high dose of green tea could cause oxidation due to its pro-oxidant activity. In conclusion, yeast C. tropicalis could be potentially used as a model cell to evaluate substances having antioxidant property in cell level.
CEKAMAN OKSIDASI SEL KHAMIR Candida tropicalis YANG DIPERLAKUKAN DENGAN PARASETAMOL DAN ANTIOKSIDAN (+)-KATEKHIN [Oxidative Stress in Candida tropicalis Treated with Paracetamol and Antioxidant (+)-catechin] Julistiono, Heddy
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.601 KB) | DOI: 10.14203/beritabiologi.v11i1.1889

Abstract

In order to more understand similarity of yeast Candida tropicalis with mammalian cells in analgesic drug paracetamol metabolism and toxicity, ability of yeast in the drug metabolism and oxidative response of cells treated with the drug and (+)-catechin was studied. In mammalian cells, paracetamol toxicity is mainly caused by metabolite byproduct of drug metabolism catalyzed by cytochrome P450, a membrane-bound enzyme and peroxidase and a soluble enzyme. Previously it has been shown that paracetamol induced oxidative stress in the yeast cells; and green tea extract protected the cells from oxidation. In this study, it had been shown that paracetamol could be metabolized by yeast cell suspension or cell free extracellular protein, reflecting possibility of role of enzyme that can not be separated from cell and that is soluble, which is common phenomenon in mammalian cell system. Paracetamol of 3 mg/ml increased lipid peroxidation, a marker of oxidative stress. A green tea polyphenol, (+)-catechin of 0.1 mg/ml did not decrease lipid peroxidation content induced by paracetamol. At higher concentration (2 mg/ml), solely (+)-catechin did not increase lipid peroxidation content. Paracetamol or (+)-catechin induced slightly activity of superoxide dismutase enzyme. The data indicated that paracetamol metabolism or toxicity in the yeast may be similar to that of mammalian cells. In this condition, it suggested that (+)-catechin is one of polyphenol green tea that has weak activity of antioxidant and consequently has weak activity of prooxidant. Mechanism of paracetamol toxicity in C. tropicalis is still to be studied with emphasis on the free radical formation and drug metabolism.
Peroksidasi Lipid oleh Parasetamol dan Ekstrak Air Panas Teh Hijau (Camellia sisnensis ) pada Sel Khamir Candida tropicalis yang di Simpan pada Suhu Rendah Julistiono, Heddy
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.376 KB) | DOI: 10.14203/jbi.v8i1.3070

Abstract

ABSTRACTLipid Peroxidation by Paracetamol and Hot Water Extract of Green Tea (Camellia sinensis ) in Yeast Candida tropicalis preserved in Low Temperature. The use of C. tropicalis cell as a tool to evaluate antioxidant property of green tea to protect oxidative stress caused by paracetamol in cell level was investigated in our laboratory. Immediate availability of cell culture will significantly reduce the time of cell preparation. Low temperature preservation of cell culture is one of methods to produce cell cultures. However, low temperature might affectphysiological state of the cell. In this study, effect of low temperature preservation (4 ºC, 10 days) on the oxidative response of yeast cell treated with paracetamol and hot water extract of green tea had been investigated. Cells incubation with paracetamol 0.3 % for 2 h caused oxidative stress in both fresh and preserved culture since the content of a marker of oxidativestress, peroxyd lipids increased significantly. Whereas, concentration of peroxidised lipids in preserved cultures was lower than that of fresh culture. Increasing of peroxydized lipids followed with decreasing of cell viability in fresh culture but not in preserved culture. Green tea withconcentration of 0.1 % decreased peroxyd lipids in fresh cultures treated with paracetamol but not in preserved cultures. Green tea with concentration of 0.2 % or 0.4 % in increased peroxyd lipids in fresh cultures treated with paracetamol but not in preserved cultures. The data indicated that green tea did not show anti- or pro-oxidative effect in cultures preserved in low temperature treated with paracetamol. However, induction of super oxide dismutase (SOD), an antioxidant defense enzyme, was not observed in cell preserved in low temperature. The study revealedthat low temperature might make the cell more resistant against prooxidative properties of paracetamol.Keywords: C. tropicalis, oxidative stress, low temperature, paracetamol, green tea
Eksplorasi Keanekaragaman Aktinomisetes Tanah Ternate Sebagai Sumber Antibiotik Nurkanto, Arif; Listyaningsih, Febrianti; Julistiono, Heddy; Agusta, Andria
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.268 KB) | DOI: 10.14203/jbi.v6i3.3141

Abstract

ABSTRACTExploration of Soil Actinomycetes Diversity from Ternate as Indigenous Antibiotic Sources.Actinomycetes of soil samples from Ternate, North Moluccas were isolated using SDS-YEmethod in humic acid vitamin agar. Ternate has high abundance of Actinomycetes, approximately6.00 – 487 x 104 CFU/ g soil, depends on habitat types. We have selected 60 isolates andconducted antibiotic screening against pathogenic bacteria and fungi using agar diffusionmethod and found both narrow and broad antibiotic spectrum types . Based on 16S rDNAanalysis, all Actinomycetes with antibiotic activities are belong to the genus Streptomyces. .Minimum Inhibitor Concentration (MIC) value was determined by broth microdilution method.It was found that MIC values varied, depended on microbial tested. We found two isolateswith higher antibiotic activity compared to the commercial antibiotics (chloramphenicol,erythromycin for antibacterial and nystatin, kabicidin for antifungal). Cell destruction analysiscaused antibiotic activities was conducted through leak of protein and nuclatic acid.Key words : Actinomycetes, soil, Ternate, antibiotic, cell distruction
Induction of Superoxide Dismutase Activities and Ethanol Resistance by Sodium Chloride and Lead Acetate in Yeast Candida sp. Y390 Julistiono, Heddy
JURNAL BIOLOGI INDONESIA Vol 4, No 1 (2006): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.188 KB) | DOI: 10.14203/jbi.v4i1.3271

Abstract

ABSTRAKAktivitas Induksi Superoksida Dismutase dan Resistensi Etanol oleh Sodium Kloridadan Plumbum Asetat pada Khamir Candida sp. Y390. Untuk mengetahui sifat khamirCandida sp. Y390 dalam melindungi diri dari cekaman oksidatif, telah dilakukan risetkajian toleransi sel terhadap etanol dan hubungannya dengan enzim superoksida dismutase(SOD) pada khamir tersebut. Viabilitas sel yang ditumbuhkan pada media gliserol dandiinkubasi pada etanol 17,s % selama 1 jam adalah 0.30 f 0.09 % , pada media gliseroldan 1 1,7 % natrium khlorida adalah 1.65 f 0.5 % sedang pada media giserol dan 1 ppmplumbum asetat adalah 1.16 f 0.7 %. Aktivitas CuZnSOD pada sel yang diinduksi olehnatrium khlorida atau plumbum asetat turun sedikit, yakni masing-masing 1,7 dan 1,9kali. Aktivitas MnSOD pada sel yang diperlakukan dengan natrium khlorida naik sedikit(sekitar 1,03 kali), namun pada sel yang diperlakukan dengan plumbum asetat naik secaramenyolok (sekitar 3,6 kali). Data ini menunjukkan bahwa metabolisme etanol dapatmenyebabkan Candida sp. Y390 mengalami cekaman oksidatif. MnSOD khamir mungkinberperan dalam melindungi sel dari kerusakan akibat cekaman oksidatif.Keywords: Candida sp. Y390, yeast, ethanol metabolism, oxidative stress, SOD.
Superoxide Dismutase Activity and Ethanol Respiration in a Fungi Resistance to Ethanol Monascus sp. MM Julistiono, Heddy; Suharna, Nandang; Desnora, Beni
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.717 KB) | DOI: 10.14203/jbi.v3i4.3319

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ABSTRACTMonascus sp. MM was a contaminant fungus isolated from museum specimenpreserved with ethanol 70 %. In order to verify role of superoxide dismutase (SOD) inprotecting cell from ethanol toxicity during ethanol metabolism, SOD activities of Monascussp.MM and a Monmcus sp. NGK, which was isolated fiom fermented red rice (angkak), werecompared. When fungus was grown with glucose, CuIZn-SOD activity of Monascus sp., MMwas 7.1 times of thht of Monascus sp. NGK. Whereas in ethanol medium, CuIZn-SOD activityof Monarcus sp. MM was 24.6 times of that in Monascus sp. NGK. Induction of CuIZn-SODMonmcus sp. MM by ethanol was not observed. Compared with Mn-SOD, activity of CuIZn-SOD was markedly important (I 0 times of Mn-SOD when fungi grown with ethanol; 12 timeswhen the fungi grown with glucose). The data indicated that Cu/Zn-SOD might play animportant role in protecting cell fiom ethanol toxicity during ethanol metabolism. Ethanolrespiration rate of Monascus sp. MM was also important since O2 consumption and ethanoldegradation rates were clearly higher than that of Monascus sp. NGK.Keywords: Monarcw sp., superoxide dismutase, respiration, ethanol resistance.
TOKSISITAS GLISEROL ATAU SUKROSA PADA SEL KHAMIR accharomyces cerevisiae YANG DISEMPAN PADA SUHU RENDAH BEKU Julistiono, Heddy
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.73 KB) | DOI: 10.14203/beritabiologi.v5i4.1123

Abstract

The effect of cryoprotectants glycerol and sucrose on cell viability and fermentation rate of Saccharomyces cerevisiae after freezing (-30 °C) and Chawing (30 °C) were studied.Both freezing and thawing were done rapidly. The mortality of cells treated with low concentrations of cryoprotectans (2.5, 5, and 10 %) after 15 and 30 days of cryopreservation, was remarkably higher than that of control and higher concentration (20% and 40%).Glycerol or sucrose with concentration of 20 % and 40 % protected cells from severe mortality only after 90 days of cryopreservation.Fermentation rate of cells treated with 20 % or 40 % of the two cryoprotectants were higher than that of control after 60 and 90 days of cryopreservation.The data indicated that in certain circumstance cryoprotectant could be toxic for the cells during freezing and thawing.Since biomembrane is not permeable to sucrose, therefore we proposed that target of sucrose toxicity may be extracellular, whereas glycerol, which penetrate cells,targets of the toxicity may be both extracellular and intracellular domains.Interaction between cryoprotectant and cell membranes is discussed.
EFEK FERRI SITRAT TERHADAP KEMAMPUAN KHAMIR Candida tropicalis DALAM MEREDUKSI 3-(4,5-DIMETHYLTHIAZOL-2-YL)- 2,5-DIPHENYLTETRAZOLIUM BROMIDE (MTT) [Effect of ferric citrate on 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) reduction in yeast Candida tropicalis] Julistiono, Heddy; Muthmainah, Resti Siti; Adam, Adam
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (533.416 KB) | DOI: 10.14203/beritabiologi.v11i2.488

Abstract

Effect of iron (ferric citrate) on 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) reduction in yeast Candida tropicalis was investigated. Reduction of MTT in yeast grown in YMB media containing 5 mM of ferric citrate decreased significantly compared to that of yeast grown containing 0, or 1.25 or 2.5 mM ferric citrate after 24 h incubation. However, there was no difference in cell density among cultures treated with 0 mM, 1.25 mM, and 5 mM ferric citrate. Ferric citrate of 5 mM caused smaller colony when cells were grown on YPDG media. Reduction of MTT in smaller colony cells was weaker than that of with normal size colony. An antioxidant, Epigallocatechin Gallate (EGCG) of 0.01 % could not reverse MTT reduction caused by 5 mM ferric citrate. Since enzymes responsible in MTT reduction are usually located in mitochondrion, the data suggested that, in the condition of 5 mM ferric citrate might cause mitochondrion disorder without killing the yeast cells. The data was in concordance with other studies on other yeast or human cells. However, this study does not show role of free radicals provoked by high level of iron concentration.
ANALISIS DELIMITASI JENIS PADA Monascus Spp. MENGGUNAKAN SIDIK JARI DNA ARBITRARY PRIMER PCR [Species Delimitation Analysis within Monascus spp. using Arbitrary Primer PCR DNA Fingerprinting] Suharna, Nandang; Julistiono, Heddy
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2629.51 KB) | DOI: 10.14203/beritabiologi.v15i2.2928

Abstract

A species delimitation analysis within Monascus spp. using Arbitrary Primer Polymerase Chain Reaction (AP PCR) DNA fingerprint was carried out. This is one of the methods used for identification and discrimination of bacterial strains within the same species. Its advantages including using single primer, independent of DNA quality, and observing amplicon shared by only some strain. This study analyzed Monascus sp. MM isolate which was originated from a source contaning high level of ethanol and two M. purpureus isolates which isolated from angkak. However, based on ITS region, 99% homology showed the unclear species delimitation. Therefore, this analysis was aimed at clarifying on the identities of Monascus species tested. The result showed DNA polymorphism among three isolates of Monascus that showed species delimitation. This study showed that species delimitation within Monascus isolates used in this analysis could be supported by AP PCR DNA fingerprinting. Therefor we suggested to use this technique or method for phylogenetic study to clarify taxonomic position of Monascus strains. 
ANALISIS VARIASI GENETIK Saccharomyces cerevisiae Dl TAHAN ETANOL DENGAN RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) [Genetic Variation Analysis of Ethanol Tolerance Yeast, Saccharomyces cerevisiae Dl by Using RAPD] Julistiono, Heddy; Yulineri, Titin; Hanjono, Sukamto
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.724 KB) | DOI: 10.14203/beritabiologi.v4i5.1240

Abstract

Genetic variations among 3 cultures, which were treated with or without Mn of Saccharomvces cerevisiae D1, were analyzed using RAPD (Random Amplified Polymorphic DNA ) technique. The Mn-treatment of three cultures were as follows: the culture KMn was D1 strain, the culture Mn+ was D1 strain colony survived in ethanol 20%, which was previously treated with 0,5 mU MnSOt and the culture Mn- was a D1 colony survived in ethanol 20% without MnSOi treatment. Polymorphism of total DNA of the three cultures may indicate that mutation may occur in cells which were tolerant to ethanol. The locus or base change was not identified. However, since the oxygen uptake rate of the three cultures at catabolite derepression state were identical,the results suggest that the locus may not be in mitochondrial DNA encoding respiratory chain proteins. The relation between DNA polimorphic and ethanol tolerant cell is still to be clarified.