Hardi Julendra
Balai Pengembangan Proses dan Teknologi Kimia (BPPTK)-LIPI, Yogyakarta

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Penggunaan Tepung Cacing Tanah (Lumbricus rubellus) sebagai Aditif Pakan terhadap Penampilan Produksi Ayam Pedaging, Profil Darah dan Kecernaan Protein (The Effect of Earthworm (Lumbricus rubellus) Meal as Feed Additive on Broiler Production Performance, Julendra, Hardi; (Zuprizal), Zuprizal; (Supadmo), Supadmo
Buletin Peternakan Vol 34, No 1 (2010): Buletin Peternakan Vol. 34 (1) Februari 2010
Publisher : Fakultas Peternakan UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | http://journal.ugm.ac.id/index.php/buletinpeternakan/article/view/103

Abstract

The objective of this research was to study the use of earthworm meal (Lumbricus rubellus) (TCT) as feed additive on broiler performance, blood profile and protein digestibility. The experiment used 100 day old chicken of cobb strain (CP 707), for 35 days feeding trial. The birds were randomly divided into four dietary treatments (R0: without TCT addition, R1: 0.5% TCT addition, R2: 1% TCT addition, R3: 1.5% TCT addition) with five replications, wich consisted five chickens each. The experimental design used Completely Randomized Design (CRD) of one way ANOVA then continued with Duncan test. Performance test was conducted by recording body weight gain, feed consumption and feed efficiency. Blood profile test was obtained by counting the number of erythrocyte, leukocyte and lymphocytes, whereas protein digestibility test used the collection method of excreta and ileum content. The result showed that feed consumption of R1 (2,065 g), R2 (2,025.4 g), R3 (2,075.3 g) were significantly different (P<0.05) fromR0 (2,309.8 g), while the body weight gain were not different among treatments and FCR of R1 (1.78), R2 (1.78), R3 (1.78) were significantly different (P<0.05) from R0 (2.04). The result of blood profile test showed that the number oferythrocyte among treatments were not significantly different, the number of leukocyte of R1 (18,575 μl), R2 (18,200 μl), R3 (20,175 μl) were found significantly higher (P<0.05) than R0 (16,675 μl) and lymphocytes of R1 (22.7%), R2 (21.8%), R3 (23.6%) were significantly higher (P<0.05) than R0 (15.2%). Protein digestibility of R3 (91.69%) was significantly different (P<0.05) from R0 (81.75%), R1 (90.48%) and R2 (85.99%), while R1 (90.48%) was significantly different (P<0.05) from R0 (81.75%), but R2 (85.99%) was not significantly different from R0 (81.75%). It could be inferred that earthworm meal addition as feed additive could improved production performance of broiler, the poultry health through blood profile, and enhanced the protein digestibility.(Key words: Feed additive, Earthworm meal, Broiler)
Inhibitory Effect of Extract Granule of Earthworms (Lumbricus rubellus) on the Pathogenic Bacteria In Vitro Istiqomah, Lusty; Damayanti, Ema; Julendra, Hardi; istika, Dewi; Winarsih, Sri
Jurnal Sain Veteriner Vol 32, No 1 (2014)
Publisher : Fakultas Kedokteran Hewan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

The objective of this study was to determine the inhibition ability of the earthworm (L. rubellus) extract (ECT), dried earthworm extract (ECT-k), and granule earthworm extract (ECT-g) as poultry feed additive against some pathogenic bacteria. Antibacterial activity was performed using diffusion method against Escherichia coli, Salmonella pullorum, Pseudomonas aeruginosa, and Staphylococcus aureus  in vitro. In the present study, the concentrations of ECT, ECT-k, and ECT-g in nutrient broth (NB) media tested were consisted of treatments A: 0%, B: 0.26%, C: 0.52%, D: 0.78% and E: 1.04% (g/vol) respectively. The results of the in vitro study showed that started from ECT level 0.26% inhibited (P <0.05) growth of P. aeruginosa and S. aureus, while ECT level 0.52% inhibited (P <0.05) E. coli and S. pullorum which proportional to the increased in concentration. ECT-k level 0.26% inhibited (P <0.05) growth of E. coli and S. aureus, while ECT-k level 0.52% inhibited (P <0.05) P. aeruginosa, and ECT-k level 1.04% inhibited (P<0.05) growth of S. pullorum. ECT-g level 0.26% inhibited (P <0.05) growth of S. pulorum, while ECT-g level 0.52% inhibited (P <0.05) S. aureus and ECT-g level 1.04% inhibited (P<0.05) growth of P. aeruginosa. There were no antibacterial action (P>0.05) of ECT and ECT-t against S. pullorum. Diameter of inhibition zone for 24 hours showed that S. aureus was the most sensitive bacterium to ECT and ECT-k, and S. pullorum was the most sensitive bacterium to ECT-g. 
The use of earthworm meal (Lumbricus rubellus) as anti-pullorum agent in feed additive of broiler chicken Damayanti, Ema; Sofyan, Ahmad; Julendra, Hardi; Untari, Tri
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 2 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.826 KB) | DOI: 10.14334/jitv.v14i2.348

Abstract

The aim of this research was to study the use of earthworm meal (TCT) L. rubellus as anti pullorum agent in poultry feed additive (IP). The antibacterial activity of TCT against Salmonella pullorum was examined using diffusion agar method at each of the following concentrations: 0, 25, 50, 75 and 100% (w/v) in 100 µL DMSO. In vivo test was conducted using 80 broiler chicken and were infected by S. pullorum with treatments of: IP0: IP contained 0% TCT, IP1: IP contained 25% TCT, IP2: IP contained 50% TCT, IP3: IP contained 75% TCT and IP4: IP contained 100% TCT. Each treatment was replicated 4 times with 4 chicks each. Feed additive was periodically fed to broiler during 7 days before and 10 days after infection. Anti-pullorum activities were evaluated using serology test, isolation and biochemical identification of S. pullorum. The results showed that 75% TCT was optimum to inhibit S. pullorum in vitro. The isolation and identification of S. pullorum results showed that 0 out of 8 (0%) broilers treated with IP4 was not infected by S. pullorum whereas 1 out of 2 (50%) broilers treated with IP0 were infected by S. pullorum. The reduction of S. pullorum prevalence as followed by increasing TCT in feed additive. In conclusion, TCT as poultry feed additive could inhibit S. pullorum infection. Key words: Earthworm Meal, Feed Additive, S. Pullorum
Antibacterial activity and retained protein of earthworm meal (Lumbricus rubellus) as feed additive combined with chitosan Sofyan, Ahmad; Damayanti, Ema; Julendra, Hardi
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 3 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v13i3.586

Abstract

This research was conducted to enhance the bacterial growth inhibition of E. coli by using earthworm (Lumbricus rubellus) meal (TCT) which was added with chitosan and its effect on the retained protein in broilers. Inhibition of E. coli growth was tested using dilution method on the nutrient broth by additional 2% TCT combined with 0, 0.5, 1.0 and 1.5% chitosan. Retained protein was measured using broiler fed diet containing 2% TCT (w/w) and added by chitosan 0, 0.5, 1.0 and 1.50% of TCT (w/w). The numbers of 15 broilers Cobb strain 35 days old were arranged on Completely Randomized Design (CRD). Results showed inhibition of E. coli was increased using TCT mixed chitosan. The highest inhibition to E. coli growth obtained from TCT + 0.5% chitosan. Retained protein tended to increase up to 1% (w/w) chitosan. Otherwise, chitosan level more than 1% could reduce protein retention. It is concluded that use of 1% chitosan increased TCT capability to inhibit E. coli and protein retention in the broilers. Key Words: Feed Additive, Chitosan, L. rubellus, E. coli
The use of earthworm meal (Lumbricus rubellus) as anti-pullorum agent in feed additive of broiler chicken Damayanti, Ema; Sofyan, Ahmad; Julendra, Hardi; Untari, Tri
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 2 (2009): JUNE 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.826 KB) | DOI: 10.14334/jitv.v14i2.348

Abstract

The aim of this research was to study the use of earthworm meal (TCT) L. rubellus as anti pullorum agent in poultry feed additive (IP). The antibacterial activity of TCT against Salmonella pullorum was examined using diffusion agar method at each of the following concentrations: 0, 25, 50, 75 and 100% (w/v) in 100 µL DMSO. In vivo test was conducted using 80 broiler chicken and were infected by S. pullorum with treatments of: IP0: IP contained 0% TCT, IP1: IP contained 25% TCT, IP2: IP contained 50% TCT, IP3: IP contained 75% TCT and IP4: IP contained 100% TCT. Each treatment was replicated 4 times with 4 chicks each. Feed additive was periodically fed to broiler during 7 days before and 10 days after infection. Anti-pullorum activities were evaluated using serology test, isolation and biochemical identification of S. pullorum. The results showed that 75% TCT was optimum to inhibit S. pullorum in vitro. The isolation and identification of S. pullorum results showed that 0 out of 8 (0%) broilers treated with IP4 was not infected by S. pullorum whereas 1 out of 2 (50%) broilers treated with IP0 were infected by S. pullorum. The reduction of S. pullorum prevalence as followed by increasing TCT in feed additive. In conclusion, TCT as poultry feed additive could inhibit S. pullorum infection. Key words: Earthworm Meal, Feed Additive, S. Pullorum
SELEKSI BAKTERI ASAM LAKTAT DENGAN AKTIVITAS ANTI JAMUR YANG DIISOLASI DARI SILASE DAN SALURAN CERNA TERNAK (Isolation of Lactic Acid Bacteria for Antifungal Activity Isolated from Silage and Animal Digestives Tract) Damayanti, Ema; Suryani, Ade Erma; Sofyan, Ahmad; Karimy, Muhammad Faiz; Julendra, Hardi
Jurnal Agritech Vol 35, No 02 (2015)
Publisher : Jurnal Agritech

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Fungi contamination was a serious problem on feed industry in Indonesia. Mycotoxin was produced by contaminated fungi could decrease feed quality and it accumulation on animal caused immunosuppressive and mortality effect. The application of biological agent such as antifungal microbe was a promising solution and to be important for futher study. The objective of this research was to select lactic acid bacteria (LAB) with antifungal activity against mycotoxin producing fungi. Lactic acid bacteria were isolated from oil palm frond (OPF) silage, poultry and ruminant digestive tracts (cattle and goat). Antifungal activities of LAB was conducted by using overlay method and paper disc diffusion method of the cell free supernatant against   FNCC 6002,   FNCC 6033 and FNCC 6111. The result showed that LAB strain PDS2 from OPF silage had the highestKeywords: Animal, lactic acid bacteria, fungi, mycotoxin, oil palm frond silage ABSTRAKKontaminasi jamur dalam bahan pakan masih menjadi masalah dalam industri ternak di Indonesia. Selain karena menurunkan kualitas pakan, akumulasi mikotoksin yang dihasilkan oleh jamur kontaminan dalam tubuh ternak juga mengakibatkan efek immunosupresif yang  menyebabkan ternak mudah terserang penyakit hingga menyebabkan kematian. Penggunaan agen biologis berupa mikrobia dengan aktivitas anti jamur menjadi solusi menjanjikan dan penting untuk dikaji. Penelitian ini bertujuan untuk menyeleksi bajteri asam laktat (BAL) dengan aktivitas anti jamur penghasil mikotoksin. BAL diisolasi dari silase pelepah sawit, saluran cerna unggas dan ruminansia (kambing dan sapi).Pengujian aktivitas anti jamur dilakukan dalam secara kualitatif dengan metode dan secara kuantitatif dengan menguji daya hambat supernatan bebas sel menggunakan metode difusi kertas cakram terhadap kapang FNCC 6002,   FNCC 6033 dan   FNCC 6111. Hasil penelitian menunjukkan isolat PDS2 dari silase memiliki daya hambat yang nyata terhadap ketiga jamur uji, sedangkan isolat BAL dari saluran cerna unggas dan ruminansia tidak menunjukkan daya hambat yang nyata.Kata kunci: Anti jamur, bakteri asam laktat, saluran cerna ternak, silase
Antibacterial activity and retained protein of earthworm meal (Lumbricus rubellus) as feed additive combined with chitosan Sofyan, Ahmad; Damayanti, Ema; Julendra, Hardi
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 3 (2008): SEPTEMBER 2008
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.843 KB) | DOI: 10.14334/jitv.v13i3.586

Abstract

This research was conducted to enhance the bacterial growth inhibition of E. coli by using earthworm (Lumbricus rubellus) meal (TCT) which was added with chitosan and its effect on the retained protein in broilers. Inhibition of E. coli growth was tested using dilution method on the nutrient broth by additional 2% TCT combined with 0, 0.5, 1.0 and 1.5% chitosan. Retained protein was measured using broiler fed diet containing 2% TCT (w/w) and added by chitosan 0, 0.5, 1.0 and 1.50% of TCT (w/w). The numbers of 15 broilers Cobb strain 35 days old were arranged on Completely Randomized Design (CRD). Results showed inhibition of E. coli was increased using TCT mixed chitosan. The highest inhibition to E. coli growth obtained from TCT + 0.5% chitosan. Retained protein tended to increase up to 1% (w/w) chitosan. Otherwise, chitosan level more than 1% could reduce protein retention. It is concluded that use of 1% chitosan increased TCT capability to inhibit E. coli and protein retention in the broilers. Key Words: Feed Additive, Chitosan, L. rubellus, E. coli
The use of earthworm meal (Lumbricus rubellus) as anti-pullorum agent in feed additive of broiler chicken Damayanti, Ema; Sofyan, Ahmad; Julendra, Hardi; Untari, Tri
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 2 (2009): JUNE 2009
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.826 KB) | DOI: 10.14334/jitv.v14i2.348

Abstract

The aim of this research was to study the use of earthworm meal (TCT) L. rubellus as anti pullorum agent in poultry feed additive (IP). The antibacterial activity of TCT against Salmonella pullorum was examined using diffusion agar method at each of the following concentrations: 0, 25, 50, 75 and 100% (w/v) in 100 µL DMSO. In vivo test was conducted using 80 broiler chicken and were infected by S. pullorum with treatments of: IP0: IP contained 0% TCT, IP1: IP contained 25% TCT, IP2: IP contained 50% TCT, IP3: IP contained 75% TCT and IP4: IP contained 100% TCT. Each treatment was replicated 4 times with 4 chicks each. Feed additive was periodically fed to broiler during 7 days before and 10 days after infection. Anti-pullorum activities were evaluated using serology test, isolation and biochemical identification of S. pullorum. The results showed that 75% TCT was optimum to inhibit S. pullorum in vitro. The isolation and identification of S. pullorum results showed that 0 out of 8 (0%) broilers treated with IP4 was not infected by S. pullorum whereas 1 out of 2 (50%) broilers treated with IP0 were infected by S. pullorum. The reduction of S. pullorum prevalence as followed by increasing TCT in feed additive. In conclusion, TCT as poultry feed additive could inhibit S. pullorum infection. Key words: Earthworm Meal, Feed Additive, S. Pullorum
Inhibitory Effect of Extract Granule of Earthworms (Lumbricus rubellus) on the Pathogenic Bacteria In Vitro Istiqomah, Lusty; Damayanti, Ema; Julendra, Hardi; istika, Dewi; Winarsih, Sri
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.22146/jsv.5427

Abstract

The objective of this study was to determine the inhibition ability of the earthworm (L. rubellus) extract (ECT), dried earthworm extract (ECT-k), and granule earthworm extract (ECT-g) as poultry feed additive against some pathogenic bacteria. Antibacterial activity was performed using diffusion method against Escherichia coli, Salmonella pullorum, Pseudomonas aeruginosa, and Staphylococcus aureus  in vitro. In the present study, the concentrations of ECT, ECT-k, and ECT-g in nutrient broth (NB) media tested were consisted of treatments A: 0%, B: 0.26%, C: 0.52%, D: 0.78% and E: 1.04% (g/vol) respectively. The results of the in vitro study showed that started from ECT level 0.26% inhibited (P <0.05) growth of P. aeruginosa and S. aureus, while ECT level 0.52% inhibited (P <0.05) E. coli and S. pullorum which proportional to the increased in concentration. ECT-k level 0.26% inhibited (P <0.05) growth of E. coli and S. aureus, while ECT-k level 0.52% inhibited (P <0.05) P. aeruginosa, and ECT-k level 1.04% inhibited (P<0.05) growth of S. pullorum. ECT-g level 0.26% inhibited (P <0.05) growth of S. pulorum, while ECT-g level 0.52% inhibited (P <0.05) S. aureus and ECT-g level 1.04% inhibited (P<0.05) growth of P. aeruginosa. There were no antibacterial action (P>0.05) of ECT and ECT-t against S. pullorum. Diameter of inhibition zone for 24 hours showed that S. aureus was the most sensitive bacterium to ECT and ECT-k, and S. pullorum was the most sensitive bacterium to ECT-g. 
Penggunaan Tepung Cacing Tanah (Lumbricus rubellus) sebagai Aditif Pakan terhadap Penampilan Produksi Ayam Pedaging, Profil Darah dan Kecernaan Protein (The Effect of Earthworm (Lumbricus rubellus) Meal as Feed Additive on Broiler Production Performance, Julendra, Hardi; (Zuprizal), Zuprizal; (Supadmo), Supadmo
Buletin Peternakan Vol 34, No 1 (2010): Buletin Peternakan Vol. 34 (1) Februari 2010
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.21059/buletinpeternak.v34i1.103

Abstract

The objective of this research was to study the use of earthworm meal (Lumbricus rubellus) (TCT) as feed additive on broiler performance, blood profile and protein digestibility. The experiment used 100 day old chicken of cobb strain (CP 707), for 35 days feeding trial. The birds were randomly divided into four dietary treatments (R0: without TCT addition, R1: 0.5% TCT addition, R2: 1% TCT addition, R3: 1.5% TCT addition) with five replications, wich consisted five chickens each. The experimental design used Completely Randomized Design (CRD) of one way ANOVA then continued with Duncan test. Performance test was conducted by recording body weight gain, feed consumption and feed efficiency. Blood profile test was obtained by counting the number of erythrocyte, leukocyte and lymphocytes, whereas protein digestibility test used the collection method of excreta and ileum content. The result showed that feed consumption of R1 (2,065 g), R2 (2,025.4 g), R3 (2,075.3 g) were significantly different (P<0.05) fromR0 (2,309.8 g), while the body weight gain were not different among treatments and FCR of R1 (1.78), R2 (1.78), R3 (1.78) were significantly different (P<0.05) from R0 (2.04). The result of blood profile test showed that the number oferythrocyte among treatments were not significantly different, the number of leukocyte of R1 (18,575 μl), R2 (18,200 μl), R3 (20,175 μl) were found significantly higher (P<0.05) than R0 (16,675 μl) and lymphocytes of R1 (22.7%), R2 (21.8%), R3 (23.6%) were significantly higher (P<0.05) than R0 (15.2%). Protein digestibility of R3 (91.69%) was significantly different (P<0.05) from R0 (81.75%), R1 (90.48%) and R2 (85.99%), while R1 (90.48%) was significantly different (P<0.05) from R0 (81.75%), but R2 (85.99%) was not significantly different from R0 (81.75%). It could be inferred that earthworm meal addition as feed additive could improved production performance of broiler, the poultry health through blood profile, and enhanced the protein digestibility.(Key words: Feed additive, Earthworm meal, Broiler)