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The Result Discrepancies between Histological and PCR Method for Detecting Helicobacter pylori in Patients with Dyspepsia due to Inappropriate Preparation before Endoscopy

The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 10, ISSUE 2, August 2009
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Abstract

Background: False negative result of Helicobacter pylori (H. pylori) detection in gastric tissue can be due to inappropriate preparation before endoscopy. The objectives of this study is to compare the result of H. pylori detection in gastric biopsy by histological method and ure C polymerase chain reaction (PCR) in patients with dyspepsia who underwent upper gastrointestinal (GI) endoscopy without preparations other than six hours fasting before endoscopy. Method: We obtained 156 paraffin blocks of gastric endoscopic biopsy samples, taken from antrum and corpus of patients with dyspepsia who underwent endoscopic examination at the Endoscopy Unit of Biomedika hospital, Mataram. All biopsy samples were stained with Hematoxylin and Eosin for tissue diagnosis and Giemsa stain for detecting H. pylori Ure C PCR were done on all blocks. Cag PCR were performed on all Ure C PCR positive samples. Results: Of 156 paraffin blocks, only 17 blocks (10.9%) were positive for H. pylori by histological examination. All of the 17 samples showed positive results on PCR method. Of 156 paraffin blocks, positive results were found in 73 patients (45.9%) by ure C PCR method. The PCR method has increased the positivity rates of H. pylori more than four times compared to histological method. This study showed that the rate of cag a was 63.0%. Conclusion: Ure C PCR is superior to histological examination in patients who did not stop consuming acid supressor drug and antibiotic two weeks prior to endoscopy. This phenomenon can be explained by the change of spiral form into coccoid form of H. pylori, which is hardly detected using Giemsa stain.   Keywords: Helicobacter pylori, histology, ureC, Cag a, PCR

The Result Discrepancies between Histological and PCR Method for Detecting Helicobacter pylori in Patients with Dyspepsia due to Inappropriate Preparation before Endoscopy

The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 10, ISSUE 2, August 2009
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Abstract

Background: False negative result of Helicobacter pylori (H. pylori) detection in gastric tissue can be due to inappropriate preparation before endoscopy. The objectives of this study is to compare the result of H. pylori detection in gastric biopsy by histological method and ure C polymerase chain reaction (PCR) in patients with dyspepsia who underwent upper gastrointestinal (GI) endoscopy without preparations other than six hours fasting before endoscopy. Method: We obtained 156 paraffin blocks of gastric endoscopic biopsy samples, taken from antrum and corpus of patients with dyspepsia who underwent endoscopic examination at the Endoscopy Unit of Biomedika hospital, Mataram. All biopsy samples were stained with Hematoxylin and Eosin for tissue diagnosis and Giemsa stain for detecting H. pylori Ure C PCR were done on all blocks. Cag PCR were performed on all Ure C PCR positive samples. Results: Of 156 paraffin blocks, only 17 blocks (10.9%) were positive for H. pylori by histological examination. All of the 17 samples showed positive results on PCR method. Of 156 paraffin blocks, positive results were found in 73 patients (45.9%) by ure C PCR method. The PCR method has increased the positivity rates of H. pylori more than four times compared to histological method. This study showed that the rate of cag a was 63.0%. Conclusion: Ure C PCR is superior to histological examination in patients who did not stop consuming acid supressor drug and antibiotic two weeks prior to endoscopy. This phenomenon can be explained by the change of spiral form into coccoid form of H. pylori, which is hardly detected using Giemsa stain.   Keywords: Helicobacter pylori, histology, ureC, Cag a, PCR

RESISTENSI PRIMER MYCOBACTERIUM TUBERCULOSIS DENGAN TARGET Gen rpoB

Jurnal Kesehatan Prima Vol 8, No 1 (2014): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

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Abstract

Abstract: Detection of primary resistance in sputum samples of patients with pulmonary tuberculosis AFB (+) with molecular analysis on the target rpoB gene causes anti-tuberculosis drug resistance (OAT) rifampicin can be beneficial to the holder of a policy program to look for tuberculosis control efforts, one of attempt to overcome these problems is to deliver information about the resistance report in this interval. This research observasional descriptive study, the research variables Mycobacterium tuberculosis rpoB gene mutations and patients with pulmonary TB sputum smear (+), with the number of 50 sputum samples with acidental sampling technique. This study results showed all the samples (50 samples) microscopic examination of smear and PCR diagnostics with primary genes and gene Tb1-Tb2 results are positive (+) tuberculosis (100%). By nested PCR amplification techniques to prove that the primary gene rpoB1 negative results of 23 samples (46%), with the primary gene rpoB2 negative results in 13 samples (26%). Conclusion: A total of 13 samples (26%) occurred mutation in the gene rpoB / is resistant to the antibiotic rifampin.

COLIFORM MOST PROBABLE NUMBER (MPN) WITH VARIETIES OF MEDIA VOLUME LACTOSE BROTH SINGLE STRENGTH (LBSS) AND LACTOSE BROTH DOUBLE STRENGTH (LBDS)

Jurnal Kesehatan Prima Vol 11, No 1 (2017): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

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Abstract

Most Probable Number (MPN) is a test that detects the fermentative nature of Coliform in the sample. The MPN test consists of three stages: presumptive test, confirm test, and completeness test. Each of these tests used LBSS, LBDS and BGLB media with volume between 5 ml - 10 ml. There is the variation of this media volume, whether it will affect the positivity value and MPN value of Coliform, it is a problem that will be answered in this study. The research objective was to find out the influence of media volume variation to the result of Coliform bacterium counting test method of MPN variety 5 1 1. The media volume which was varied namely LBSS and LBDS with volume variation 4 m, 6 ml, 8 ml, 10 ml, 12 Ml. The research was an experimental in laboratory. The sample was the suspense of Escherichia coli bacteria which are equivalent to standard 0.5 Mc. Farland and diluted 1000 ml sterile aquadest, the result data indicated MPN value was obtained, then it is analyzed by descriptive analysis. The findings indicated that the volume of LBDS and LBSS media were 4ml, 6ml, 8ml, 10ml, 12ml, replicated 5 times obtained positive result on all tubes 5 1 1 with MPN value> 265/100 ml. The conclusion of the research is media volume of LBSS and LBDS does not affect the positive and MPN Coliform values.

PERBEDAAN HASIL UJI KOAGULASE MENGGUNAKAN PLASMA SITRAT MANUSIA 3,8%, PLASMA SITRAT DOMBA 3,8%, DAN PLASMA SITRAT KELINCI 3,8% PADA BAKTERI Staphylococcus aureus

Jurnal Kesehatan Prima Vol 9, No 2 (2015): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

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Abstract

Abstract: Coagulase test is a bacterial examination performed to detect the formation of coagulase enzyme bound to the bacterial cell wall. This study aims to determine the difference in the form of a coagulase test results clot formation by using several types of plasma citrate in bacteria Staphylococcus aureus. This type of research is true experimental replication using 9 and 3 human citrate plasma treatment is 3.8%, 3.8% sheep plasma citrate, and 3.8% rabbit citrate plasma. The results show the average time of the formation of clots in the coagulase test using 3.8% human citrate plasma was 1:29 minutes, using sheep plasma citrate 3.8% was 00:54 minutes, and using 3.8% rabbit citrate plasma is 1:40 minutes. Results of One Way Anova statistical test obtained p value count (0,00)

GOLD IMMUNOCHROMATOGRAPHIC ASSAY (GICA) SEBAGAI IMUNOSENSOR MENDETEKSI ANTIBODI Bacillus Anthracis PENYEBAB PENYAKIT ZOONOSIS

Jurnal Kesehatan Prima Vol 9, No 1 (2015): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

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Abstract

Abstract: The purpose of this study was to identify patterns of protein antigens full / secretory antigens may be antigenic protein (PA) of Bacillus anthracis by the use of SDS-PAGE method (Laemmli) which can be used as bioreseptor on the test device fabrication method for detecting antibodies GICA of Bacillus anthracis , identify patterns of proteins that are antigenic protein (PA) or indicate the nature of the reactivity of Bacillus anthracis by using Western blotting and Determining the specificity and sensitivity of the test method to detect antibodies GICA Bacillus anthracis when using the results of the ELISA method as a reference. This study is a descriptive observational study. Research variable antigenic protein of Bacillus anthracis as bioreseptor and Antibodies from Bacillus anthracis as imunosenssor. The test prototype test device made using 20 sample with ELISA as a reference for calculating the sensitivity and specificity. The results of this study were identified band – band of 92 kDa, 68 kDa, 54 kDa, 43 kDa, 29 kDa and 18 kDa that can be used as bioreseptor on GICA, identified a pattern of proteins that are antigenic or indicate the nature of the reactivity of the Bacillus anthracis 68 kDa by Western blotting using the method, extract secretory antigen (SA) Bacillus anthracis can be used as a test device bioreseptor on GICA method for the detection of IgG antibodies against Bacillus anthracis and sensitivity and specificity of the test method to detect antibodies GICA Bacillus anthracis when using the results of the ELISA method as a reference each is 100% and 87.5%.

PENGARUH PERBEDAAN SUHU TERHADAP POTENSI TABLET SIPROFLOKSASIN (EKSPERIMEN DENGAN BAKTERI Escherichia coli)

Jurnal Kesehatan Prima Vol 9, No 2 (2015): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

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Abstract

Abstract: It has been conducted the determination of potency of Ciprofloxacin 500 tablets was storage in temperature 0°C, 10°C, 20°C, 30°C, 40°C for 20 hour. The aim of research is to find out the effect of different temperature for potency of Ciprofloxacin tablets. The method of determination applied is the measure diameter zone inhibition Escherichia coli in MHA. The result indicated that was negative interaction temperature storage for potency of Ciprofloxacin tablets.

DRAGON FRUIT (Hylocereus polyrhizus) AS NATURAL DYES STAINING FOR BACTERIA

Jurnal Kesehatan Prima Vol 10, No 2 (2016): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

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Abstract

Abstract :  the bacteria is difficult to be perceived with a light microscope because it can not adsorb or refract the light, consequently the dye applies for staining the bacteria or their background. Synthetic dye has a fairly expensive price than natural dye. This study aims to determine the usage of dragon fruit (Hylocereus polyrhizus) as a natural dye for staining the bacteria. This research is quasi experimental, while the data will be analysed by descriptive analysis with 3 treatments, in each uses eight samples. The study showed that on the quality of stains, bacterium Staphylococcus aureus and E. coli by using the juice of the dragon is obtained a result, as many as 4 slides preparations of bacteria Staphylococcus aureus (33.33%) and 4 slides preparations of bacteria E .coli (33.33%) were not stained. Therefore, the result on the research can be concluded that the dragon fruit (Hylocereus polyrhizus) can not be used as a natural dye for staining bacteria.

NATURAL MEDIA FOR THE GROWTH OF CANDIDA ALBICANS CAUSES OF CANDIDIASIS BY ARTOCARPUS COMMUNIS

Jurnal Kesehatan Prima Vol 11, No 2 (2017): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

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Abstract

a candida albicans requires a source of nutrients especially carbohydrates that are sufficient for its growth. Semi-synthesis media is the most common medium for growing Candida albicans. Semi-synthesized media composes of natural and synthesis materials. An abundance of biological sources in nature with higher carbohydrate content than natural ingredients on semi-synthesized media encourage to find the materials of alternative media from different carbohydrate sources, particularly grains, one of them is breadnut seed. This study aimed to find out a potential of Artocarpus communis as an alternative media for the growth of Candida albicans causes of candidiasis. This study was true experiment, used 6 replications and 4 treatments with PDA as control media, the media of Artocarpus communis with concentration of 10%, 20%, and 30%. The data of Candida albicans growth was tested statistically used One Way Anova with 95% confidence level or α = 0.05. The result indicated that a number of Candida albicans growth on PDA media was 13.7 colonies, on alternative media of Artocarpus communis with the concentration of 10% was an average of 13.5 colonies, the concentration of 20% was an average of 16.3 colonies and the concentration of 30% was an average of 24 colonies. The conclusion: the Artocarpus communis can be employed as an alternative natural media of Candida albicans growth.

TOTAL KOLESTEROL CONDITIONS ON CONSUMPTION OF BLACK POWDER COFFEE (Traditional) WITH PATIENT PREPARATION

Jurnal Kesehatan Prima Vol 12, No 1 (2018): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

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Abstract

Abstract: A traditional black coffee powder is one of the beverages that excessively consumed by communities. The increasing of total cholesterol level in blood is influenced by the habit of consuming coffee. Coffee has chemicals that can cause some health problems, namely cafestol and kahweol. The objective study was to determine the effect of patient preparation against the result of total cholesterol levels examination on the consumers of traditional black coffee powder. This research was analytic observational. The sample was capillary blood of consumers’ a traditional black coffee as many as 30 people. Statistical analysis utilized Mann-Whitney U Test with 95% confidence level (α = 0,05). The findings indicated the average deviation of total cholesterol level after fasting 12,86 mg / dL and mean difference after giving coffee 7 cups 18,46 mg / dL. Mann Whitney Test found significant value 0.001 <α = 0.05 which means there is an influence of patient preparation against the result of total cholesterol levels examination on the consumers of traditional black coffee powder.Keywords: Total Cholesterol Level; Consumers of Traditional Black Coffee Powder.