Riris Istighfari Jenie
Fakultas Farmasi, UGM, Sekip Utara Yogyakarta 55281

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The Safety of Areca Seed Ethanolic Extract as Potential Chemopreventive Agent is Proven by Acute Toxicity Test Handayani, Sri; Jenie, Riris Istighfari; Susidarti, Ratna Asmah
Indonesian Journal of Cancer Chemoprevention Vol 3, No 1 (2012)
Publisher : Indonesian Research Gateway

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Abstract

Areca (Areca catechu L.) seeds ethanolic extract (AE) exhibits antiproliferative activity and induces apoptosis on T47D and MCF-7 cells. This study aimed to verify AE safety using acute toxicity test to support its development as chemopreventive agent. Male Sprague Dawley Rat 9Rattus norvegicus) age 8 weeks divided into five groups, one group of control treated with 0.5 % CMC-Na only and four groups for treatment. Single dose in oral administration was done to test animal with various dose of AE starts from lowest dose to highest dose expected toxic to all of test animal (0.1; 0.72; 5.36 and 10 gram/kgBW). Observation was done during 24 hours and continued for 14 days. The observation criteria were toxic symptoms, appearance and mechanism of toxic effect and pathology of vital organ. Histopathology analysis of some vital organs was done with Haematoxyllin & Eosin (H&E) staining. Toxic effect did not appear either on treatment groups or control group. Treatment of single dose of areca ethanolic extract, even in highest dose, did not cause the death of the animals. Therefore, observation extended to 14 days and terminated by necroption of the animals. All of group did not show histopathological alterations in microscopic observation. Category of the potential toxicity of AE is practically non-toxic, ie 10 g/kgBW. The result show the safety of areca seed ethanolic extract which is important for its development as chemopreventive agent.Key words: Areca catechu, acute toxicity, rat
Combination of Tangeretin and 5-Fluorouracil Modulates Cell Cycle and Induce Apoptosis on Widr Cells Indriyani, Luthfia; Hermawan, Adam; Jenie, Riris Istighfari
Indonesian Journal of Cancer Chemoprevention Vol 3, No 1 (2012)
Publisher : Indonesian Research Gateway

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Co-chemotherapeutics approaches are increasing in cancer treatment in order mainly to suppress the resistance phenomenon of cancer treatment and to enhance the cytotoxic effect of the main chemotherapeutics agent. Tangeretin has been known to have cytotoxic effect to some cancer cells through some pathways in the cells. To explore the potential effect of tangeretin as co-chemotherapeutics agent this research was subjected to study the cytotoxic  effect of tangeretin in combination with 5-Fluoro Uracil (5-FU) on WiDR colon cancer cells covering the modulation of cell cycle and apoptosis induction. Cytotoxic effect was examined by using MTT assay while apoptosis induction was determined by annexin-V flowcytometry. Under MTT assay, tangeretin showed weak cytotoxic activity on the cells. However, tangeretin significantly enhanced the cytotoxic effect of 5-FU on the cells. This co-chemotherapeutics effect likely correlated with cell cycle modulation effect, especially in inducing polyploidy phenomenon as expressed in the flowcytometric graph of the DNA content. This combination also increased apoptosis induction. These result suggest that tangeretin is potential to be developed as co-chemotherapeutic agent for 5-FU on colon cancer and further molecular mechanism need to be exploredKeywords : Tangeretin, 5-Fluorourasil, WiDr, cell cycle, apoptosis
Hesperidin Increases Cytotoxic Effect of 5-Fluorouracil on WiDr Cells Gilang, Yurista; Hermawan, Adam; Fitriasari, Aditya; Jenie, Riris Istighfari
Indonesian Journal of Cancer Chemoprevention Vol 3, No 2 (2012)
Publisher : Indonesian Research Gateway

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Therapy  of  colon  cancer  by  using  5-FU  often  causes  problems  of  resistance.  This encourages the development of co-chemotherapy agent. One of the compounds that could potentially be used as a co-chemotherapy agent  is hesperidin. This study was conducted to determine the cytotoxic effects of hesperidin, 5-FU and the combination of them, as well as apoptosis induction in colon cancer cells WiDr. Cytotoxic effect of hesperidin, 5-FU, and its combination were observed using MTT assay. Observation of apoptosis was done by double staining method using ethidium bromide-acridin orange. Until 48 hours incubation, hesperidin showed no cytotoxic effects. Cytotoxic effects of 5-FU was observed after 48 hours with the IC50 value of 422 µM. However, hesperidin improved the cytotoxic effects of 5-FU at 48 hour incubation.  Either  single  treatment  of  hesperidin  200µM  or  5-FU  1500  µM  did  not  trigger apoptosis, but combination of them led to the emergence of signs of apoptosis. Based on this study,it can be concluded thathesperidin is potential to be developed as a co-chemotherapy agent of 5-FU on colon cancer but still need further study on its molecular mechanisms.Keywords : hesperidin, 5-fluorouracil, WiDr cells, cytotoxic, apoptosis
Co-chemotherapy of sambung nyawa (Gynura procumbens (Lour.) Merr.) leaves ethanolic extract and Doxorubicin on breast cancer cell Meiyanto, Edy; Jenie, Riris Istighfari
INDONESIAN JOURNAL OF PHARMACY Vol 18 No 2, 2007
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (421.883 KB) | DOI: 10.14499/indonesianjpharm0iss0pp81-87

Abstract

Chemotherapy combination attracted high attention in recent years to cure cancer. This method combines between non-toxic or less toxic phytochemicals and chemotherapeutic agents to sensitize cancer cell, to enhance the efficacy of chemotherapeutic agents as well as to reduce its toxicity to normal tissues. The aim of the present research was to examine whether ethanolic extract of Sambung Nyawa leaves (SNE) or Gynura procumbens (Lour.) Merr. synergizes the therapeutic potential of doxorubicin (Dox) on breast cancer cell line T47D.MTT assay was used to measure the effect of growth inhibitory of the combination therapy on T47D cells. SNE (25-500 μg/mL) treatment of cell resulted in 12-97.% growth inhibition in a dose dependent manner (IC50 90 μg/mL), while Dox (1.8-90 nM) treatment showed inhibitory effect of 16-83.% (IC50 50 nM). The combinations of SNE with Dox (1.8-18 nM) showed synergistic effect (CI<1). These results demonstrate a strong possibility of synergistic efficacy of SNE and Dox combination for breast cancer treatment.Key words: Sambung Nyawa leaves ethanolic extract, doxorubicin, cochemotherapy, T47D human mammary cancer cell.
Snake beans (Vigna sinensis (L) Savi ex Hassk) extract increases breast epithelial cells proliferation Meiyanto, Edy; Handayani, Sri; Jenie, Riris Istighfari
INDONESIAN JOURNAL OF PHARMACY Vol 19 No 4, 2008
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1596.989 KB) | DOI: 10.14499/indonesianjpharm0iss0pp191-197

Abstract

Some Javaness people use snake beans for skin and breast care, especially for better breast development. This research was conducted to examine the effect of snake beans extract (EKP) on the breast epithelial cells proliferation on in vitro and in vivo models. The in vitro experiment was carried out against MCF-7 cells using MTT assay and morphologically examination was carried out under light microscope. Sprague Dawley female Rats were used in in vivo experiment. The rats (30 days of age) were separated into 3 groups, namely base line group, control group, and treatment groups. The extract was administered in the dose of 1000 mg/kgBW p.o. every day for 14 days then the rats were examined for wet uterus weight, lobulus development, and estrogen receptor (ER) expression. Extract treatment induced MCF-7 cells proliferation in dose dependent manner. The extract exhibited proliferative effect in the dose of 50 ug/mL 300 ug/mL, but in the dose of 400 ug/mL and 500 ug/mL the extract inhibited cells proliferation and there were no cell death effect. Extract treatment in the dose of 1000 mg/kgBW tended to increase uterus weight. The extract also increased lobulus development up to two fold and induced estrogen receptor expression in epithelial cells of lobulus and ductus. These results conclude, snake bean (in appropriate dose) induces breast glands development and relatively safe (no death effect on the cells), therefore can be developed for breast care product.Key words: Snake bean, breast care, proliferation, lobulus epithelial cells, estrogen receptor
Antiangiogenic effect of sambung nyawa leaves (Gynura procumbens (Lour.) Merr.) etanolic extract on chick embryo chorioallantoic membrane (CAM) Jenie, Riris Istighfari; Meiyanto, Edy; Murwanti, Retno
INDONESIAN JOURNAL OF PHARMACY Vol 17 No 1, 2006
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.767 KB) | DOI: 10.14499/indonesianjpharm0iss0pp50-55

Abstract

Antiangiogenesis (inhibition of new blood vessels formation) has become a strategy to inhibit cancer development lack of nutrition and oxygen supply. The aim of the present research is to investigate antiangiogenesis effect of ethanolic extract of Gynura procumbens (Lour.) Merr. Leaves in situ using chick embryo chorioallantoic membrane (CAM). Eight to 9 days old fertilized chicken eggs were treated with b-FGF (angiogenesis inductor) and extracts. Eggs were then incubated for 3 days in order to observe its angiogenesis response (new blood vessels converged toward the implant).The results showed that the ethanolic extract of G.procumbens could inhibit angiogenesis in a dose-dependent manner. Doses 10, 20, 40, 80 ug gave angiogenesis response of (in percent) 82.32 ± 6.33; 68.38 ± 6.24; 56.48 ± 11.61; 41.43 ± 7.46 (p<0.05), respectively. These results indicate a potential antiangiogenic effect of the extract.Key words: antiangiogenic, CAM, G.procumbens.
SYNTHESIS AND CYTOTOXIC ACTIVITY OF 2,5-BIS(4-BORONIC ACID)BENZYLIDINE CYCLOPENTANONE ON HER2 OVEREXPRESSED-CANCER CELLS Utomo, Rohmad Yudi; Putri, Herwandhani; Pudjono, Pudjono; Susidarti, Ratna Asmah; Jenie, Riris Istighfari; Meiyanto, Edy
INDONESIAN JOURNAL OF PHARMACY Vol 28 No 2, 2017
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (949.546 KB) | DOI: 10.14499/indonesianjpharm28iss2pp74

Abstract

Development of chemotherapeutic agent and boron carrying pharmaceutical based on HER2 specific targeted is important due to its role in enhancing cancer progression. The purpose of this study is to synthesize curcumin analogue, namely Pentagamaboron-0 (PGB-0) or 2,5-bis(4-boronic acid)benzylidine cyclopentanone, and to explore the cytotoxic activity on HER2 overexpressed-cancer cells. MCF-7/HER2 was used as a model of HER2 overexpressed-cancer cells and NIH3T3 as normal cells. PGB-0 bound to ATP binding site of HER2 and EGFR based on molecular docking study. PGB-0 was synthesized resulting in 33% yield and was confirmed by IR, 1HNMR, 13CNMR and Mass spectroscopy. Based on MTT assay PGB-0 decreased cells viability on MCF-7/HER2 cells with IC50 value of 270 µM but performed no effect on NIH3T3 cells. Cell cycle analysis revealed that PGB-0 increased sub-G1 accumulation. PGB-0 decreased HER2 expression in a dose-dependent manner. We conclude that the new compound PGB-0 inhibits cell growth through cell death induction and decreased HER2 expression. Thus, PGB-0 is potential to be developed as a chemotherapeutic agent and boron carrying pharmaceutical targeted on the HER2 receptor.
APLIKASI KO-KEMOTERAPI FRAKSI ETIL ASETAT EKSTRAK ETANOLIK DAUN SAMBUNG NYAWA (GYNURA PROCUMBENS (LOUR.) MERR.) PADA SEL KANKER PAYUDARA MCF-7 Jenie, Riris Istighfari; Meiyanto, Edy
Pharmaceutical Sciences and Research (PSR) Vol 6, No 3 (2009)
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (81.17 KB) | DOI: 10.7454/psr.v6i3.3442

Abstract

Combination chemotherapy has been an interesting attention in recent years to cure cancer e.g. non-toxic or less toxic phytochemicals are being combined with chemo-therapeutic agents to sensitize cancer cell and to enhance the efficacy of chemothera-peutic agents as well as to reduce its toxicity to normal tissues. The aim of this research is to examine whether ethyl acetate fraction of Gynura procumbens ethanolicextract (SEF) synergizes the therapeutic potential of doxorubicin (Dox) on breast cancer cell line MCF-7. MTT  assay were used to measure the growth inhibitory effect of the combination therapy on MCF-7 cells. SEF (5-250  µg/ml) treatment of cellresulted in 15-76% growth inhibition in a dose dependent manner (IC50 85 µg/ml), while Dox (10-100 nM) treatment did not show any inhibitory effect. The combina-tions of SEF (5-40µg/ml) with Dox (10-75 nM) seemed to not have any synergistic efficacy towards cell growth inhibition. Nevertheless, this result need further observa-tion regarding the IC50 of Dox on MCF-7 has not been determined yet. The cellcharacterization may influence the result. Doxorubicin could induce Akt survival apoptosis pathway in MCF-7 resulting resistancy of the cell towards doxorubicin.Key words: MCF-7 human mammary cancer cell, Doxorubicin, Sambung Nyawaleaves ethanolic extract, co-chemotherapy.
Cytoprotective effect of tangeretin in hydrogen peroxyde-inducedoxydative stress on human umbilical vein endothelial cells (HUVECs) Jenie, Riris Istighfari; Setiawan, Irfan Muris; Jenie, Ikhlas Muhammad; Muflikhasari, Haruma Anggraini
Jurnal Ilmiah Farmasi Vol 11, No 1 (2015): Jurnal Ilmiah Farmasi
Publisher : Jurnal Ilmiah Farmasi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol11.iss1.art3

Abstract

Oxydative stress is known as a major trigger in cardiovascular dysfunction that leads to hypertension, atherosclerosis, and chronic heart failure. Polymethoxyflavone such as tangeretin and nobiletin has been reported to have biological activity including reducing cholesterol level and anti inflammation activity. The critical point that mediates atherosclerosis is the formation of stress oxidative which causes endothelial cells dysfunction.This study was conducted to explore the effect of tangeretin in protecting H2O2-induced oxidative stress on HUVEC. Cell viability was determined by using MTT assay. Cells were treated with different concentrations of tangeretin (12.5; 100; and 200 µM) for 24h before exposed to 600 µM H2O2 for 4h. Cells viability analyses showed that at low concentration (12.5 µM), tangeretin inhibited H2O2-induced cytotoxicity. Furthermore, it induced cells proliferation. However, at high concentration, it failed to protect HUVEC from H2O2-induced cytotoxicity and increased the cytotoxicity of H2O2. Based on this result, tangeretin in low concentration has a potential to reduce oxidative stress on HUVECs.  
Brazilein in combination with cisplatin inhibit proliferation and migration on highly metastatic cancer cells, 4T1 Handayani, Sri; Susidarti, Ratna Asmah; Udin, Zalinar; Meiyanto, Edy; Jenie, Riris Istighfari
Indonesian Journal of Biotechnology Vol 21, No 1 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.26106

Abstract

Brazilein performs anti­cancer activities on several cancer cells and potentially inhibits metastasis. The aims of this study is to observe the synergistic cytotoxic and migration inhibitory effect of brazilein combined with cisplatin on 4T1 breast cancer cells. Under MTT assay, we found that brazilein revealed cytotoxic effect on 4T1 cells in a dose­dependent manner (IC50=50 ± 0.3 µM). Combination of brazilein and cisplatin showed synergistic effect (CI=0.72). Flowcytometry analysis on the cell cycle progression showed that single treatment of 25 µM brazilein induced G2/M­phase accumulation, 12.5 µM cisplatin induced S­phase accumulation, while combination of brazilein and cisplatin induced S­phase and G2/Mphase accumulation. Combination of brazilein and cisplatin induced apoptosis higher than that of the single treatments. Based on wound healing assay, 12.5 µM brazilein and its combination with 6.25 µM cisplatin inhibited cells migration. Immunoblotting and gelatin zymography analysis showed that combination of brazilein and cisplatin inhibited the expression level of Rac1 and MMP9 proteins. Based on these results, we conclude that brazilein enhanced cytotoxic activity of cisplatin and inhibited migration on 4T1 cells and potentially can be developed as an enhancing cytotoxic and antimetastasis agent.