Riris Istighfari Jenie
Fakultas Farmasi, UGM, Sekip Utara Yogyakarta 55281
Articles
9
Documents
ARECA (Areca catechu L.) SEEDS ETHANOLIC EXTRACT AND ITS CHLOROFORM FRACTION INCREASE APOPTOTIC EFFECT OF DOXORUBICIN ON HUMAN COLON CANCER CELLS

Jurnal Bahan Alam Indonesia Vol 6, No 5 (2008)
Publisher : Indonesian Research Gateway

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Abstract

Pada penelitian terdahulu, ekstrak etanolik biji buah pinang (AE) dan fraksi kloroformnya (ACF) memiliki aktifitas sitotoksik pada sel kanker kolon WiDr dengan nilai IC50 masing-masing sebesar 124 μg/mL dan 119 μg/mL. Penelitian ini bertujuan untuk mengetahui efek kombinasi AE dan ACF dengan agen kemoterapi Doxorubicin (Dox), serta efek kombinasi tersebut dalam menginduksi apoptosis pada sel kanker WiDr.Serbuk biji buah pinang diekstraksi menggunakan etanol 96%. Kemudian ekstrak difraksinasi secara partisi menggunakan heksan dan klorofon untuk mendapatkan fraksi klorofom. Aktivitas sitotoksik baik AE, ACF dan Dox tunggal maupun kombinasi dilakukan dengan metode MTT. Pengamatan apoptosis menggunakan metode double staining (acrydine orange-ethidium bromide). Metode imunositokimia digunakan untuk mendeteksi ekspresi Bcl-2 dan COX-2.Kombinasi AE dan ACF dengan Dox menunjukkan efek sinergistik pada sel kanker WiDr dengan indeks kombinasi (Cl) <0.9 (P<0.05). Pada kombinasi Dox - AE, efek sinergis paling kuat terlihat pada kombinasi Dox 100 dan 334 nM dengan AE 60 μg/mL (Cl<0.1), sedangkan efek sinergis paling kuat pada kombinasi Dox-ACF terlihat pada kombinasi Dox 100 nM dengan ACF 30,40 dan 60 μg/mL (Cl=0.1). Kedua kombinasi tersebut mampu  menginduksi apoptosis pada sel kanker WiDr. Kombinasi Dox-ACF mampu menurunkan ekspresi Bcl-2 dan meningkatkan ekspresi Bax. Hasil tersebut menunjukkan apoptosis pada sel kanker WiDr yang dimungkinkan mekanismenya berhubungan dengan ekspresi Bcl-2 dan COX-2. 

The Safety of Areca Seed Ethanolic Extract as Potential Chemopreventive Agent is Proven by Acute Toxicity Test

Indonesian Journal of Cancer Chemoprevention Vol 3, No 1 (2012)
Publisher : Indonesian Research Gateway

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Abstract

Areca (Areca catechu L.) seeds ethanolic extract (AE) exhibits antiproliferative activity and induces apoptosis on T47D and MCF-7 cells. This study aimed to verify AE safety using acute toxicity test to support its development as chemopreventive agent. Male Sprague Dawley Rat 9Rattus norvegicus) age 8 weeks divided into five groups, one group of control treated with 0.5 % CMC-Na only and four groups for treatment. Single dose in oral administration was done to test animal with various dose of AE starts from lowest dose to highest dose expected toxic to all of test animal (0.1; 0.72; 5.36 and 10 gram/kgBW). Observation was done during 24 hours and continued for 14 days. The observation criteria were toxic symptoms, appearance and mechanism of toxic effect and pathology of vital organ. Histopathology analysis of some vital organs was done with Haematoxyllin & Eosin (H&E) staining. Toxic effect did not appear either on treatment groups or control group. Treatment of single dose of areca ethanolic extract, even in highest dose, did not cause the death of the animals. Therefore, observation extended to 14 days and terminated by necroption of the animals. All of group did not show histopathological alterations in microscopic observation. Category of the potential toxicity of AE is practically non-toxic, ie 10 g/kgBW. The result show the safety of areca seed ethanolic extract which is important for its development as chemopreventive agent.Key words: Areca catechu, acute toxicity, rat

Combination of Tangeretin and 5-Fluorouracil Modulates Cell Cycle and Induce Apoptosis on Widr Cells

Indonesian Journal of Cancer Chemoprevention Vol 3, No 1 (2012)
Publisher : Indonesian Research Gateway

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Abstract

Co-chemotherapeutics approaches are increasing in cancer treatment in order mainly to suppress the resistance phenomenon of cancer treatment and to enhance the cytotoxic effect of the main chemotherapeutics agent. Tangeretin has been known to have cytotoxic effect to some cancer cells through some pathways in the cells. To explore the potential effect of tangeretin as co-chemotherapeutics agent this research was subjected to study the cytotoxic  effect of tangeretin in combination with 5-Fluoro Uracil (5-FU) on WiDR colon cancer cells covering the modulation of cell cycle and apoptosis induction. Cytotoxic effect was examined by using MTT assay while apoptosis induction was determined by annexin-V flowcytometry. Under MTT assay, tangeretin showed weak cytotoxic activity on the cells. However, tangeretin significantly enhanced the cytotoxic effect of 5-FU on the cells. This co-chemotherapeutics effect likely correlated with cell cycle modulation effect, especially in inducing polyploidy phenomenon as expressed in the flowcytometric graph of the DNA content. This combination also increased apoptosis induction. These result suggest that tangeretin is potential to be developed as co-chemotherapeutic agent for 5-FU on colon cancer and further molecular mechanism need to be exploredKeywords : Tangeretin, 5-Fluorourasil, WiDr, cell cycle, apoptosis

Hesperidin Increases Cytotoxic Effect of 5-Fluorouracil on WiDr Cells

Indonesian Journal of Cancer Chemoprevention Vol 3, No 2 (2012)
Publisher : Indonesian Research Gateway

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Abstract

Therapy  of  colon  cancer  by  using  5-FU  often  causes  problems  of  resistance.  This encourages the development of co-chemotherapy agent. One of the compounds that could potentially be used as a co-chemotherapy agent  is hesperidin. This study was conducted to determine the cytotoxic effects of hesperidin, 5-FU and the combination of them, as well as apoptosis induction in colon cancer cells WiDr. Cytotoxic effect of hesperidin, 5-FU, and its combination were observed using MTT assay. Observation of apoptosis was done by double staining method using ethidium bromide-acridin orange. Until 48 hours incubation, hesperidin showed no cytotoxic effects. Cytotoxic effects of 5-FU was observed after 48 hours with the IC50 value of 422 µM. However, hesperidin improved the cytotoxic effects of 5-FU at 48 hour incubation.  Either  single  treatment  of  hesperidin  200µM  or  5-FU  1500  µM  did  not  trigger apoptosis, but combination of them led to the emergence of signs of apoptosis. Based on this study,it can be concluded thathesperidin is potential to be developed as a co-chemotherapy agent of 5-FU on colon cancer but still need further study on its molecular mechanisms.Keywords : hesperidin, 5-fluorouracil, WiDr cells, cytotoxic, apoptosis

Co-chemotherapy of sambung nyawa (Gynura procumbens (Lour.) Merr.) leaves ethanolic extract and Doxorubicin on breast cancer cell

INDONESIAN JOURNAL OF PHARMACY Vol 18 No 2, 2007
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

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Abstract

Chemotherapy combination attracted high attention in recent years to cure cancer. This method combines between non-toxic or less toxic phytochemicals and chemotherapeutic agents to sensitize cancer cell, to enhance the efficacy of chemotherapeutic agents as well as to reduce its toxicity to normal tissues. The aim of the present research was to examine whether ethanolic extract of Sambung Nyawa leaves (SNE) or Gynura procumbens (Lour.) Merr. synergizes the therapeutic potential of doxorubicin (Dox) on breast cancer cell line T47D.MTT assay was used to measure the effect of growth inhibitory of the combination therapy on T47D cells. SNE (25-500 μg/mL) treatment of cell resulted in 12-97.% growth inhibition in a dose dependent manner (IC50 90 μg/mL), while Dox (1.8-90 nM) treatment showed inhibitory effect of 16-83.% (IC50 50 nM). The combinations of SNE with Dox (1.8-18 nM) showed synergistic effect (CI<1). These results demonstrate a strong possibility of synergistic efficacy of SNE and Dox combination for breast cancer treatment.Key words: Sambung Nyawa leaves ethanolic extract, doxorubicin, cochemotherapy, T47D human mammary cancer cell.

Snake beans (Vigna sinensis (L) Savi ex Hassk) extract increases breast epithelial cells proliferation

INDONESIAN JOURNAL OF PHARMACY Vol 19 No 4, 2008
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

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Abstract

Some Javaness people use snake beans for skin and breast care, especially for better breast development. This research was conducted to examine the effect of snake beans extract (EKP) on the breast epithelial cells proliferation on in vitro and in vivo models. The in vitro experiment was carried out against MCF-7 cells using MTT assay and morphologically examination was carried out under light microscope. Sprague Dawley female Rats were used in in vivo experiment. The rats (30 days of age) were separated into 3 groups, namely base line group, control group, and treatment groups. The extract was administered in the dose of 1000 mg/kgBW p.o. every day for 14 days then the rats were examined for wet uterus weight, lobulus development, and estrogen receptor (ER) expression. Extract treatment induced MCF-7 cells proliferation in dose dependent manner. The extract exhibited proliferative effect in the dose of 50 ug/mL 300 ug/mL, but in the dose of 400 ug/mL and 500 ug/mL the extract inhibited cells proliferation and there were no cell death effect. Extract treatment in the dose of 1000 mg/kgBW tended to increase uterus weight. The extract also increased lobulus development up to two fold and induced estrogen receptor expression in epithelial cells of lobulus and ductus. These results conclude, snake bean (in appropriate dose) induces breast glands development and relatively safe (no death effect on the cells), therefore can be developed for breast care product.Key words: Snake bean, breast care, proliferation, lobulus epithelial cells, estrogen receptor

Antiangiogenic effect of sambung nyawa leaves (Gynura procumbens (Lour.) Merr.) etanolic extract on chick embryo chorioallantoic membrane (CAM)

INDONESIAN JOURNAL OF PHARMACY Vol 17 No 1, 2006
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

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Abstract

Antiangiogenesis (inhibition of new blood vessels formation) has become a strategy to inhibit cancer development lack of nutrition and oxygen supply. The aim of the present research is to investigate antiangiogenesis effect of ethanolic extract of Gynura procumbens (Lour.) Merr. Leaves in situ using chick embryo chorioallantoic membrane (CAM). Eight to 9 days old fertilized chicken eggs were treated with b-FGF (angiogenesis inductor) and extracts. Eggs were then incubated for 3 days in order to observe its angiogenesis response (new blood vessels converged toward the implant).The results showed that the ethanolic extract of G.procumbens could inhibit angiogenesis in a dose-dependent manner. Doses 10, 20, 40, 80 ug gave angiogenesis response of (in percent) 82.32 ± 6.33; 68.38 ± 6.24; 56.48 ± 11.61; 41.43 ± 7.46 (p<0.05), respectively. These results indicate a potential antiangiogenic effect of the extract.Key words: antiangiogenic, CAM, G.procumbens.

ARECA (Areca catechu L.) SEEDS ETHANOLIC EXTRACT AND ITS CHLOROFORM FRACTION INCREASE APOPTOTIC EFFECT OF DOXORUBICIN ON HUMAN COLON CANCER CELLS

Jurnal Bahan Alam Indonesia Vol 6, No 5 (2008)
Publisher : Indonesian Research Gateway

Show Abstract | Original Source | Check in Google Scholar

Abstract

Pada penelitian terdahulu, ekstrak etanolik biji buah pinang (AE) dan fraksi kloroformnya (ACF) memiliki aktifitas sitotoksik pada sel kanker kolon WiDr dengan nilai IC50 masing-masing sebesar 124 μg/mL dan 119 μg/mL. Penelitian ini bertujuan untuk mengetahui efek kombinasi AE dan ACF dengan agen kemoterapi Doxorubicin (Dox), serta efek kombinasi tersebut dalam menginduksi apoptosis pada sel kanker WiDr.Serbuk biji buah pinang diekstraksi menggunakan etanol 96%. Kemudian ekstrak difraksinasi secara partisi menggunakan heksan dan klorofon untuk mendapatkan fraksi klorofom. Aktivitas sitotoksik baik AE, ACF dan Dox tunggal maupun kombinasi dilakukan dengan metode MTT. Pengamatan apoptosis menggunakan metode double staining (acrydine orange-ethidium bromide). Metode imunositokimia digunakan untuk mendeteksi ekspresi Bcl-2 dan COX-2.Kombinasi AE dan ACF dengan Dox menunjukkan efek sinergistik pada sel kanker WiDr dengan indeks kombinasi (Cl) <0.9 (P<0.05). Pada kombinasi Dox - AE, efek sinergis paling kuat terlihat pada kombinasi Dox 100 dan 334 nM dengan AE 60 μg/mL (Cl<0.1), sedangkan efek sinergis paling kuat pada kombinasi Dox-ACF terlihat pada kombinasi Dox 100 nM dengan ACF 30,40 dan 60 μg/mL (Cl=0.1). Kedua kombinasi tersebut mampu  menginduksi apoptosis pada sel kanker WiDr. Kombinasi Dox-ACF mampu menurunkan ekspresi Bcl-2 dan meningkatkan ekspresi Bax. Hasil tersebut menunjukkan apoptosis pada sel kanker WiDr yang dimungkinkan mekanismenya berhubungan dengan ekspresi Bcl-2 dan COX-2. 

APLIKASI KO-KEMOTERAPI FRAKSI ETIL ASETAT EKSTRAK ETANOLIK DAUN SAMBUNG NYAWA (GYNURA PROCUMBENS (LOUR.) MERR.) PADA SEL KANKER PAYUDARA MCF-7

Pharmaceutical Sciences and Research (PSR) Vol 6, No 3 (2009)
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

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Abstract

Combination chemotherapy has been an interesting attention in recent years to cure cancer e.g. non-toxic or less toxic phytochemicals are being combined with chemo-therapeutic agents to sensitize cancer cell and to enhance the efficacy of chemothera-peutic agents as well as to reduce its toxicity to normal tissues. The aim of this research is to examine whether ethyl acetate fraction of Gynura procumbens ethanolicextract (SEF) synergizes the therapeutic potential of doxorubicin (Dox) on breast cancer cell line MCF-7. MTT  assay were used to measure the growth inhibitory effect of the combination therapy on MCF-7 cells. SEF (5-250  µg/ml) treatment of cellresulted in 15-76% growth inhibition in a dose dependent manner (IC50 85 µg/ml), while Dox (10-100 nM) treatment did not show any inhibitory effect. The combina-tions of SEF (5-40µg/ml) with Dox (10-75 nM) seemed to not have any synergistic efficacy towards cell growth inhibition. Nevertheless, this result need further observa-tion regarding the IC50 of Dox on MCF-7 has not been determined yet. The cellcharacterization may influence the result. Doxorubicin could induce Akt survival apoptosis pathway in MCF-7 resulting resistancy of the cell towards doxorubicin.Key words: MCF-7 human mammary cancer cell, Doxorubicin, Sambung Nyawaleaves ethanolic extract, co-chemotherapy.