Diana Krisanti Jasaputra
Fakultas Kedokteran Universitas Kristen Maranatha

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Deteksi Mycobacterium tuberculosis dengan Teknik PCR pada Cairan Efusi Pleura Penderita Tuberkulosis Paru Jasaputra, Diana Krisanti; Widjaja, Jahja Teguh; Wargasetia, Teresa Liliana; Makangiras, Iryanthy
Jurnal Kedokteran Maranatha Vol 7, No 1 (2007)
Publisher : Universitas Kristen Maranatha

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Tuberculosis pleural effusion is a manifestation of  extrapulmonal tuberculosis, which is rampant in various countries in  the world, including Indonesia. Nowadays one-third of the world population has been infected by tuberculosis. Every year there are approximately 8 million new tuberculosis patients throughout the world and nearly 3 million people die of this disease every year. Pathogenesis of tuberculosis pleural effusion is a pleural hypersensitivity reaction accompanied by a small number of  Mycobacterium tuberculosis. The objective of this research was to detect  Mycobacterium tuberculosis in pleural effusion liquid by means of PCR technique to establish diagnosis of tuberculosis pleural effusion. On this research, detection of Mycobacterium tuberculosis by PCR technique was compared with microscopic examination by means of Ziehl-Neelsen (ZN) method. The research result showed that 9 out of 11 samples were positive by using PCR technique whereas with ZN method all samples showed negative result. The conclusion of this research was that Mycobacterium tuberculosis in small numbers could be detected in pleural effusion liquid by means of PCR technique so that diagnosis could certainly be established.
Efek Antiinflamasi dan Keamanan Phyllanthus niruri L. Herba dan Taraxacum officinale Weber et Wiggers Herba terhadap Dermatitis Alergika pada Mencit Jasaputra, Diana Krisanti; ., Rosnaeni
Jurnal Kedokteran Maranatha Vol 7, No 1 (2007)
Publisher : Universitas Kristen Maranatha

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Allergic dermatitis is a common disease  in community, and is still a health problem because of its recurency. Phyllanthus niruri L. Herba and Taraxacum officinale Weber et Wiggers Herba are often used  in treating Atopic Dermatitis (AD). The objectives of this research are to determine antiinflammation effect of n-hexane fraction of these herb  to  Allergic dermatitis in mice and its  safety. A longitudinal prospective research using Randomized Complete Design with Swiss Webster adult male mice strain as experimental animal. The examination of the antiinflammation effect use 30 mice divided in 6 group (n=5). Each gruop induced by Ovalbumin at day 1,7, and 21, except the negative controle group. N-hexane fraction of these herb was given orally at day 21. The measured data consitsts of  the inflammation coverage area, the number of inflammation cells microscopically at histology preparate with HE and the percentage of eosinophyl in peripheral blood swab. The acute toxicity test  was carried on 80 male mice  and 80 female mice . There are differences in the  inflammation coverage area and the number of inflammation cells on the mice induced by Ovalbumin group (positive control, 27,30 mm2, 247, p<0,05) and the mice group received Ovalbumin and n-hexane fraction of the two herbs with dose 1 (12,85 mm2, 142), dose 2 (5,09 mm2, 53), and dose 3 (15,28 mm2, 116). Eosinophyl percentage on the test group with dose 1 (1%) and dose 2 (2%) are significantly different from the positive control (10%)(p<0,05).However, the eosinophyl percentage on the test group with dose 3 (10%) is insignificantly different from the positive control (p>0,05). The toxicity test of the two herbs showed There are no toxicity until dose 16 000 mg/KgBW. The conclusion are  n-hexane fraction of Phyllanthus niruri L. Herba and Taraxacum officinale Weber et Wiggers Herba have an antiinflammation effect on mice with Allergic Dermatitis  and are safe for oral consumption.
Akurasi Deteksi Mycobacterium tuberculosis dengan Teknik PCR menggunakan “Primer X” dibandingkan dengan Pemeriksaan Mikroskopik (BTA) dan Kultur Sputum Penderita dengan Gejala Tuberkulosis Paru Jasaputra, Diana Krisanti; Onggowidjaja, Philips; Soeng, Sylvia
Jurnal Kedokteran Maranatha Vol 5, No 1 (2005)
Publisher : Universitas Kristen Maranatha

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Akhir-akhir ini, terjadi peningkatan jumlah penderita tuberkulosis. Guna pembe-rantasan penyakit TBC, diagnosis dan deteksi Mycobacterium tuberculosis menjadi amat penting. Deteksi tersebut dapat dilakukan dengan teknik Polymerase Chain Reaction (PCR), pemeriksaan mikroskopik, dan kultur bakteri. Tujuan penelitian ini adalah menilai sensitivitas, spesifisitas, dan akurasi deteksi Mycobacterium tuberculosis dalam sputum penderita TBC paru dengan teknik PCR dibandingkan pemeriksaan secara mikroskopik (Bakteri Tahan Asam/BTA) dan kultur bakteri TBC dalam sputum.Penelitian ini merupakan suatu uji diagnostik yang dirancang secara cross sectional. Penelitian dilakukan terhadap penderita TB paru di BP4 Jl Cibadak Bandung, mulai April 2004 sampai dengan Agustus 2004.Pemeriksaan sputum penderita dilakukan dengan tiga teknik pemeriksaan, yaitu dengan teknik PCR, pemeriksaan BTA secara mikroskopik, dan kultur bakteri.Dibandingkan dengan pemeriksaan Bakteri Tahan Asam secara mikroskospik, deteksi Mycobacterium tuberculosis dengan teknik PCR memiliki sensitivitas 30%, spesifisitas 80%, dan akurasi 47%. Uji kemaknaan dengan Mc Nemar memberikan hasil adanya perbedaan yang bermakna. (p < 0,01).Dibandingkan dengan metode kultur bakteri TBC, deteksi Mycobacterium tuberculosis dengan teknik PCR memiliki sensitivitas 65%, spesifisitas 40%, dan akurasi 57%. Uji kemaknaan dengan Mc Nemar memberikan hasil tidak adanya perbedaan yang bermakna. (p = 1,0). Dibandingkan dengan pemeriksaan Bakteri Tahan Asam secara mikroskospik, deteksi Mycobacterium tuberculosis dengan metode kultur bakteri TBC memiliki sensitivitas 31,6%, spesifisitas 81,8%, dan akurasi 50%. Uji kemaknaan dengan Mc Nemar memberikan hasil adanya perbedaan yang bermakna. (p < 0,01).Dengan demikian, dapat disimpulkan bahwa deteksi Mycobacterium tuberculosis dengan teknik PCR sama baiknya dengan kultur bakteri TBC, namun waktu pemeriksaan dengan teknik PCR lebih singkat dibandingkan dengan kultur bakteri TBC. Mycobacterium tuberculosis banyak tidak terdeteksi dengan pemeriksaan mikroskopik (BTA)
Imunomodulator pada Penyakit Alergi Jasaputra, Diana Krisanti
Jurnal Kedokteran Maranatha Vol 4, No 2 (2005)
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Penyakit-penyakit alergi seperti asma, pilek alergi dan dermatitis alergika adalah penyakit-penyakit yang patofisiologinya didasari oleh ketidakseimbangan sistem imun, yang mana terjadi dominasi jalur Th2.Oleh karena itu, terapi imunomodulator yang mengurangi aktivitas sel-sel imun pada jalur Th2 sebaiknya digunakan untuk mengatasi berbagai manifestasi penyakit alergi.Terapi imunomodulator yang telah digunakan antara lain adalah imunoterapi. Beberapa tumbuhan obat seperti Phyllanthus niruri, Physalis angulata, Smilax officinalis, dan Pfaffia paniculata dapat pula memodulasi sel-sel imun yang aktif secara berlebihan. Selain itu, imunomodulator seperti Methotrexate, Cyclosporine, dan intravenous immunoglobulin (IVIG) yang telah digunakan untuk mengatasi berbagai penyakit gangguan sistem imun mungkin dapat digunakan juga. Namun penggunaan ketiga obat tersebut untuk mengatasi penyakit alergi khususnya asma masih sangat terbatas, karena keterbatasan data yang menunjang penggunaannya dan potensi obat-obat tersebut untuk menimbulkan efek samping.
Gangguan Sistem Imun pada Anak Autistik Jasaputra, Diana Krisanti
Jurnal Kedokteran Maranatha Vol 2, No 2 (2003)
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Autisme pada anak merupakan gangguan perkembangan yang muncul pada usia batita (bawah tiga tahun), yang menyebabkan mereka tidak mampu membentuk hubungan sosial atau mengembangkan komunikasi normal.Salah satu kelainan yang dijumpai pada anak autistik adalah gangguan sistem imun antara lain (1) Defisiensi Sistem Imun seperti defisiensi enzim myeloperoxidase, defek pada limfosit T dan Limfosit B, defisiensi Ig A, yang berfungsi untuk melindungi sepanjang dinding saluran cerna terhadap paparan benda asing, dan defisiensi komplemen C4b. (2) Kelainan Autoimun, dengan dibentuknya antibodi terhadap infeksi virus baik yang alamiah maupun yang berasal dari vaksin dan antibodi terhadap infeksi jamur seperti Candida albicans, yang mana antibodi tersebut dapat bereaksi silang dengan myelin otak, atau jaringan otak sehingga terjadi gangguan perkembangan saraf anak-anak autistik. dan (3) Fenomena alergi makanan pada anak autistik yang mana gangguan ini didasari oleh adanya defisiensi sistem imun dalam saluran pencernaan yang akan menyebabkan peningkatan pertumbuhan organisme seperti jamur dan masuknya benda-benda asing termasuk alergen makanan ke dalam berbagai bagian tubuh yang lain yang menimbulkan reaksi alergi dengan manifestasi antara lain gangguan perilaku seperti yang dijumpai pada autism spectrum disorder.Salah satu cara penanggulangan defesiensi sistem imun adalah dengan pemberian IVIG. Penanggulangan terhadap pertumbuhan Candida albicans perlu dilakukan pula dengan pemberian diet, suplementasi makanan tertentu, dan beberapa obat-obat seperti nystatin, ketoconazole, dan kadang-kadang amphotericin B dapat diberikan dengan dosis sangat rendah per oral. Probiotik, seperti lactobacillus Gg dapat diberikan untuk menjaga flora usus dalam keadaan seimbang. Penanggulangan alergi makanan, sebagai salah satu akibat defisiensi sistem imun, yang paling penting adalah eliminasi alergen makanan dari diet penderita. Makanan-makanan yang dipantang ini sebaiknya ditentukan dengan tes alergi misalnya dengan pemeriksaan Ig E RAST dalam darah. Obat-obat anti alergi dan anti radang seperti antihistamin penghambat reseptor H1 dan H2, ketotifen, kortikosteroid, dan penghambat sistesis prostaglandin dapat diberikan.
Etnopharmacology Observation of Medicinal Plant/Traditional Medicinal Ingredient for Dyslipidemia Treatment in West Java Local Wisdom Wiwaha, Guswan; Budiastuti, Sarifudin Niken; Jasaputra, Diana Krisanti; Rohmawaty, Enny; Yunivita KD, Vycke; Muchtar, Elvy
Jurnal Medika Planta Vol 2, No 1 (2012)
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Introduction: SP3T (Sentra Pengembangan Penerapan Pengobatan Tradisional) West Java traditional medicinal plants to identify efficacious anti-dyslipidemia, because (1) Indonesia has a wealth of medicinal plant materials, (2) provisions of the Government of Indonesia to improve the utilization of medicinal plants and its data base, (3) and an increase in the use of plant drugs in the world.Objective: to know the use of medicinal plants by traditional healers (BATTRA) for dyslipidemia. Methods: This study identifies plants used by BATTRA to proceed with study dyslipidemia etnofarmakologis through in-depth interviews with community leaders and resource practitioners / academics universities (anthropologist, botanical expert and sociologist) to acquire pharmaceutical products containing indigenous Sundanese Society.Results: The term dyslipidemia by BATTRA called diseases associated with obesity cholesterol.; Various medicinal herb or plant a single drug has been used by such BATTRA: betel leaf and Mahkotadewa (Sukabumi), bitter, antanan, temuputih, ginger, and honey habbatussaudah (Falkirk), celery, white radish, apples, tangerines, and brown sugar (Sumedang), the main quality of green tea, the leaves mimbau, Culan girlfriend, ginger, ginger and turmeric (Cimahi), SATEKOLA which consisted of a bitter, ginger, komprey, and pepper (Tasikmalaya), antanan, leaves gods, and continued life (Bekasi), noni seeds (Indramayu), mangosteen (Garut). Conclusions antanan plants, continued life and leaves gods agreed as medicinal plants / herbs that are indigenous Sundanese community in West Java.
Protective Effect of Ethanol Extract Purwoceng to Phone Electromagnetic Wave Exposure as a Risk Factor for Andropouse Jasaputra, Diana Krisanti; Herdiman, Heddy; -, Elvin
Jurnal Medika Planta Vol 2, No 1 (2012)
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Introduction: Andropause is now well accepted that serum testosterone (T) levels decline progressively with aging in men and circumstances related to exposure to free radicals, including exposure from mobile phone. Purwoceng been used by communities for generations to enhance virility. Objective: to assess protective effect of purwoceng in overcoming the negative impact of the concentration of cell phones from the concentration of spermatozoa.Method: The research method used is true experimental design in laboratorium with completely randomized design. This research used 24 male mice divided into 4 groups (n = 6), namely: Group I as negative control; Group II, mice were given only the phone electromagnetic waves; Group III, mice were given 32 mg / kg Purwoceng extracts; Group IV, mice were given 32 mg / kg Purwoceng extracts and providing mobile phone electromagnetic waves. Parameters which are measured were concentration of spermatozoa. The analysis data by one-way ANOVA and Tukey HSD test with α = 0.05. Result: the average concentration of spermatozoa (million/mm3) in group I (3.97), group II (0.37), group III (6.43), and group IV (4.4). Group II and III are significantly different than the control group with p <0.05, while the fourth group is not significant compared with the negative control group.Conclusion: an electromagnetic wave radiation cell phone decrease the sperm concentrations and extracts Purwoceng has protective effect to cell phone electromagnetic waves radiation though spermatozoa concentration parameters. And this study indicates that Purwoceng has a positive effect in reducing the symptoms of andropause.
THE Th1/Th2 IMBALANCE, ATOPIC ECZEMA, AND HERBAL MEDICINE Jasaputra, Diana Krisanti; Kurniawati, Dewi; Baskara, Tri Budhi
Jurnal Medika Planta Vol 1, No 1 (2010)
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People with immune system imbalance tent to get allergic diseases, autoimmune diseases, carcinoma, and other diseases. People with atopic disease have too many Th2 defenders and a lack of Th1 defenders. Immune system imbalance can be caused by environmental stress, such as eating an unnatural diet, being exposed to certain bacteria, breathing in polluted air, drinking chemically processed water, living under psychological pressure. These can produced oxidative stress, that can be treated using antioxidant, such as garlic and tumeric, so that Th2 dominant be reduced and atopic eczema symptom be reduced too. 
THE COMPARISON OF EFFECT BETWEEN WATER AND ETHANOL EXTRACT OF MENIRAN HERB ON THE AMOUNT OF EOSINOPHIL PERCENTAGE IN ATOPIC DERMATITIS MICE MODEL Jasaputra, Diana Krisanti; Santosa, Slamet; Sujatno, Muchtan
Jurnal Medika Planta Vol 1, No 1 (2010)
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Introduction: Atopic dermatitis is hypersensitivity reaction type I which is followed by the increasing of eosinophil, which has role in pathogenesis chronic allergic inflammation. One of the treatment target of atopic dermatitis is to decrease the amount of eosinophil, so the inflammation reaction decreased. Meniran herb is usually used by community to treat dermatitis. The aim of this research is to find out the effect of water and ethanol extract of meniran herb (Phyllanthus niruri. L) to the amount of eosinophil percentage. Methods: The experimental preclinic research in laboratorium is longitudinal, prospective, and comparatives. The mice that induced allergy with ovalbumin were divided randomly in groups (n=6). Various doses of  water and ethanol extract meniran herb were given to those groups, then compared to the group which is given the ovalbumin only (control positive). The experimental data of the amount of eosinophil percentage was analyzed by ANOVA, which followed by the Duncan test, with α=0,05. Results: the research proved that decreasing of the amount of eosinophil percentage doses 1, 2, 3, 4, water meniran extract consecutively 34.833%, 16.167%, 22.833%, 27.833% whereas doses 1, 2, 3, 4, etanol meniran extract consecutively 31.167%, 21.667%,35.167%, 25.833% compared with the positive control (46.833%) (p<0,05). Water and ethanol extract have the same maximum effectivity at dose 2 (p>0,05). Conclusion: meniran herb water and ethanol extract have same effect in decreasing the amount of eosinophil percentage.
ANTIINFLAMMATORY EFFECT OF DANDELION INTO ATOPIC DERMATITIS MODELS USING MICE Jasaputra, Diana Krisanti; Liana, Laella K.; Sujatno, Muchtan; Soen, Mary
Jurnal Medika Planta Vol 1, No 1 (2010)
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Introduction: Atopic dermatitis is an inflammation of the skin based on hypersensitivity type I reaction. Medication for atopic dermatiis can be done in conventional or inconventional ways and dandelion (Taraxacum officinale Weber et Wiggers) is one of them. Objective: to determine antiinflammatory effect of dandelion’s aqueous and ethanol extract in reducing the inflammation area and the number of inflammatory cells on mice’s skin tissue with atopic dermatitis induced by ovalbumin. Methods: The characteristic of this research was prospective experimental laboratory, using mice, injected with 0.2 ml ovalbumin 10% intracutaneously, on the first, seventh, and twenty-first days. The mice were given dandelion’s aqueous and ethanol extract with dose 1: 0.0078g/ mice 20g; dose 2: 0.0195g/ mice 20g;  dose 3: 0.039g/ mice 20g; dose 4: 0.078 g/ mice 20g perorally. Results: The results show that dandelion’s aqueous extract with dose 4 (6.640mm2) and ethanol extract with dose 2 (5.013mm2)  were the most effective doses in reducing the inflammation area (p<0,05). The summary result of the number of inflammation cells shows statistically significant on giving the dandelion’s ethanol extract dose 1 and 2 compared with the positive control (p<0,05). The effectivity of ethanol extract equal to the aqueous extract in reducing the inflammation coverage area and the number of inflammation cells. Conclusion: both dandelion’s aqueous and ethanol extract are effective in reducing the inflammation coverage area, ethanol extract effective to reduce the number of inflammation cells. Both dandelion’s aqueous and ethanol extract are equally effective.