HEDI INDRA JANUAR
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Analysis of Bacterial Community Associated with Aaptos sp. from Rote and Seribu Islands CHASANAH, EKOWATI; PATANTIS, GINTUNG; DEWI, ARIYANTI SUHITA; MARRASKURANTO, ENDAR; JANUAR, HEDI INDRA; STELLA, STELLA; SOKA, SUSAN; YOGIARA, YOGIARA
Microbiology Indonesia Vol 7, No 1 (2013): March 2013
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.7.1.37

Abstract

Aaptos sp. is a marine sponge that could produce bioactive compounds such as aaptamin, aaptosin, and isoaaptamin which have activities as antitumor, antimicrobial, and antiviral. Community of bacteria associated with the sponge might correlate with production of those bioactive compounds and be affected  by  water environment where the sponge grow. The presence of anthropogenic stressor such as pollutans might become a burden to the waters where the biota grown and could affect the microbial biodiversity in the sponge and its active metabolite produced. The objective of this research was to analyze bacterial community associated with Aaptos sp. from Rote Island and Seribu Islands, using T-RFLP method. The results showed that bacterial community associated with Aaptos sp. from both sampling sites shared 40.81% similarity in which they were dominated by the same bacteria class of Actinobacteria, Flavobacteria, α-proteobacteria, δ-proteobacteria, and γ–proteobacteria. The bacteria collected from Rote island  were more highly distributed and diverse than those from Seribu Islands. A total of 23 classes of microorganism were identified in Rote Island waters, while in Seribu Islands was 14 classes of microorganism. The presence of Actinobacteria and Proteobacteria in Aaptos sp., is allegedly involved in the production of secondary metabolites.
Peta Keanekaragaman Bakteri Spons Aaptos sp. di Perairan Taman Nasional Kepulauan Seribu (Bacterial Diversity Map in Aaptos sp. from Seribu Islands National Parks) Patantis, Gintung; Januar, Hedi Indra; Zilda, Dewi Seswita
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 18, No 3 (2013): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (540.566 KB)

Abstract

Senyawa metabolit pada spons diketahui memiliki keterkaitan dengan mikroba baik yang bersimbion maupun dalam lingkungan sekitarnya. Perubahan lingkungan baik yang disebabkan oleh pengaruh antropogenik maupun perubahan iklim dapat berpengaruh terhadap lingkungan perairan yang secara tidak langsung dapat mempengaruhi struktur komunitas bakteri di lingkungan tersebut. Penelitian ini dilakukan untuk mengetahui peta keanekaragaman bakteri yang berasosiasi dengan Aaptos sp. Sampel yang diuji adalah Aaptos sp. dan air dari wilayah perairan Taman Nasional Kepulauan Seribu (TNKpS). Analisis keragaman bakteri dilakukan menggunakan teknik Terminal Restriction Fragment Length Polymorphism (T-RFLP). Hasil penelitian dari 8 lokasi pengambilan sampel Aaptos sp. memperlihatkan bahwa bakteri di wilayah ini memiliki rentang indek keanekaragaman  Shannon-Weiner (H) sebesar: 0,12–2,56, kekayaan (R): 4-29, dan kerataan (E): 0,09–0,78. Sementara itu, rentang H, R, dan E dari air berturut-turut: 1,28-1,97; 6-17; 0,58-0,90. Analisis lanjutan menunjukan tidak adanya korelasi yang signifikan antara indeks keanekaragaman bakteri Aaptos sp. dan air di perairan TNKpS (R= 0,310 pada P<0,01). Peta keanekaragaman bakteri menunjukan wilayah dengan keanekaragaman tertinggi adalah di perairan utara dan selatan TNKpS. Hal ini selaras dengan kualitas perairan terbaik di wilayah perairan tersebut, oleh karena itu kondisi lingkungan yang baik perlu terus dijaga agar kekayaan alam yang terkandung didalamnya juga tetap terjaga. Kata kunci: peta, keanekaragaman, bakteri, T-RFLP, Taman Nasional Kepulauan Seribu  Sponge metabolites were reported to be related with their associated microbes and the environment where the sponges grow. Environment changes caused by anthropogenic stressor and climate changes can affect the waters, and indirectly can affect bacterial community structure in the environment and in the sponges. The aim of this study was to determine the map of the bacteria diversity associated with Aaptos sp. Samples tested were Aaptos sp. and water from the Seribu Islands National Park (TNKpS). Bacterial diversity analysis was conducted using Terminal Restriction Fragment Length Polymorphism (T-RFLP) technique. The results showed that the diversity of bacteria in Aaptos sp. from the 8 sampling sites had a range of Shannon-Weiner diversity index (H) of 0.12 to 2.56, richness (R) of 4-29, and evenness (E) of 0.09 to 0.78. Meanwhile, the range of H, R, and E of waters were 1.28-1.97; 6-17; 0.58 to 0.90, subsequently. Further analysis showed that no significant correlation between bacterial diversity index of Aaptos sp. and water in the TNKpS waters (R = 0.310 at P<0.01). Diversity map showed that the areas with highest bacterial diversity were in the north and south of TNKpS waters. This was consistent with the best water quality in these areas, therefore environmental conditions is crucial to maintain its natural resources. Keywords: map, diversity, bacteria, T-RFLP, Seribu Islands National Parks
CORRELATION DIVERSITY OF BACTERIA TO SECONDARY META BOLITES Nephthea spp. from SERIBU ISLANDS NATIONAL PARKS WATERS Patantis, Gintung; Januar, Hedi Indra; Chasanah, Ekowati
Widyariset Vol 15, No 3 (2012): Widyariset
Publisher : LIPI-Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (832.743 KB)

Abstract

Bioactive compounds from biota on coral reef is potentially of plasma nutfah that has high economic value.Their existence was influenced by the microbes which associated with them and the environmental conditions. Theaim of the research was to analyze the correlation between the diversity of bacteria and secondary metabolitesNephthea spp. collected from Seribu Islands National Parks (TNKpS). The analysis of bacteria diversity performedby Terminal Restriction Fragment Length Polymorphism (T-RFLP) and secondary metabolites used High PerformanceLiquid Chromatography (HPLC). The results of study showed that the range of diversity index (H), richness(R), and evenness index (E) of bacteria were 1,47–0,88; 13–3; 0,84–0,43. And the for the secondary metabolitesthe range were 2,21–1,12; 20-5; 0,95–0,63. Further study showed that there was a significant correlation (R =0.96 at p< 0.01) between bacteria and secondary metabolites group richness. The area which high diversity ofbacteria and secondary metabolites were within central and southern TNKpS waters. This results were in line withthe best water quality in these areas.
Cembranoids Content of Soft Coral Sarcophyton from Acidified Environment at Volcano Island, Indonesia Januar, Hedi Indra; Zamani, Neviaty Putri; Soedharma, Dedi; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 1 (2017): May 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (413.038 KB) | DOI: 10.15578/squalen.v12i1.276

Abstract

Cembranoid content in soft coral is known as a chemotype that relate with genotype and environment. This research aimed to characterize the cembranoid Sarcophyton soft coral from the reef that acidified by CO2 volcanic vents (pHT 7.8) at Volcano Islands waters, Banda-Neira (Indonesia), as a means of predicting the future impact of ocean acidification to the genetic diversity of Sarcophyton soft coral. 30 random colonies were taken, combined, and extracted with ethanol. Cembranoid isolation and identification had been done by high performance liquid chromatography and spectroscopic techniques. Results of the study found sarcophytol derivatives (sarcophytol A, 11,12-epoxy sarcophytol A, sarcophytol B, and sarcophytol M) as the only chemotype in the sample. This may suggest low genetic diversity in the observed Sarcophyton sample. Therefore, it may suggest that even soft coral is known to be resilient to future acidification pressures, the genetic diversity or the production of diverse cytotoxic metabolite may be hampered due to ocean acidification in future climate change adaptation.
Fatty Acid Profile, Carotenoid Content, and In Vitro Anticancer Activity of Karimunjawa and Lampung Sea Cucumber Chasanah, Ekowati; Fawzya, Yusro Nuri; Tarman, Kustiariyah; Januar, Hedi Indra; Nursid, Muhammad
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 3 (2016): December 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1039.624 KB) | DOI: 10.15578/squalen.v11i3.269

Abstract

Fatty acids and carotenoid has been known as an anticancer agent on both preventing and treating cancer disease. This study was conducted to analyze the fatty acid profile, carotenoid and in vitro anticancer activity of 12 sea cucumber harvested from Karimunjawa and Lampung waters. The aim of the study was to determin the potency of sea cucumbers as raw material for nutraceutical products. Fatty acid profile and carotenoid content were characterized by gas chromatography and spectrophotometry techniques, while in vitro anticancer activity was assessed by MTT assay against cervix (HeLa), breast (T47D and MCF-7) and colon (WiDR) cancer cells. Results of the study showed polyunsaturated fatty acid (PUFA) dominated the composition of fatty acids in the samples from both locations. Holothuria sp. was detected to contain the highest amount of carotenoid. Furthermore, the highest in vitro anticancer activity was detected also in the sample of Holothuria sp. The activity of 30 ppm Holothuria sp. extract against HeLa cell was detected to be almost equal to the 5 ppm doxorubicin control. Concentration of 5 ppm Holothuria sp. extract also showed positive result in killing 50% of MCF-7 and T47D, but capable to 100% kill HeLa and WiDR cells. At concentration of 25 ppm, the extract was able to kill all the 4 cells tested. Statistical analysis showed the amount of carotenoid and two particular fatty acid compounds (docosadienoic and eicosapentaenoic acid) significantly (P<0.05) contributed to the cytotoxic activity that was found in the sea cucumber samples. Those compounds were found in highest concentration from Holothuria sp harvested from Lampung waters, thus being the most prospective raw material for nutraceutical or functional food ingredient with anticancer potency.
Correlation between fucoxanthin contents in Turbinaria sp.and sea water nutrients at Binuangeun and Krakal Coasts Januar, Hedi Indra; Wikanta, Thamrin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 6, No 1 (2011): May 2011
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (388.043 KB) | DOI: 10.15578/squalen.v6i1.57

Abstract

Fucoxanthin pigment is a potential compound in nutraceutical. It is commonly found in algaesuch as in Turbinaria sp.,a very abundant species in Indonesian coastal. To maximize theproduction of fucoxanthin for the development of nutraceutical industry in the coastal area, it isimportant to identify the optimal environmental parameters for its biosynthesis. It has been reportedthat nitrogen and or phosphate nutrients affect the growth of algae. Therefore, this paper aimed toobtain an optimal environment for Turbinaria sp. to produce fucoxanthin based on correlationanalysis. Correlated data were fucoxanthin isolated from Turbinaria sp.that was collected fromKrakal (Yogyakarta) and Binuangeun (Banten) coasts. Fucoxanthin contents in each sample werecompared based on the peak areas in chromatogram after 1 mg injection of n-hexane extract ofT. decurrrens in the HPLC system. The analysis results showed that the peak areas of fucoxanthinwere significantly different among sampling locations (P = 7.99 x 10-6), sampling periods (P = 6 x10-3) and interaction of both (P = 3 x 10-3). Whereas the correlation analysis revealed that interactionbetween phosphate and temperature could be a predictor that strongly (R = 0.986) and significantlycorrelated (P = 1.09 x 10-7) with the peak areas of fucoxanthin (R2= 96.5%). These results were inline with growth limiting factor in seawater and the function of fucoxanthin as ultraviolet protector.Based on these results, it is suggested that the suitable location for fucoxanthin production is in thearea of agricultural run-off that contains an elevated concentration of dissolved phosphate.
STUDY ON THE EFFECT OF POLLUTANTS ON THE PRODUCTION OF AAPTAMINES AND THE CYTOTOXICITY OF CRUDE EXTRACT FROM Aaptos suberitoides Dewi, Ariyanti Suhita; Hadi, Tri Aryono; Januar, Hedi Indra; Pratitis, Asri; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 3 (2012): December 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (640.121 KB) | DOI: 10.15578/squalen.v7i3.4

Abstract

This experiment was conducted to study the effects of anthropogenic stressor on the spatialvariability of secondary metabolites from marine sponge Aaptos suberitoides. Samplings wereconducted at 7 sites in Marine National Park of Thousand Islands that are extended within 30 kmoff Jakarta bay on late February 2011. Sponges were collected and quantified by means of liquidchromatography coupled with photo-diode array detection, whereas, cytotoxicity of sponges extractswas determined against T47D (breast) cancer cell lines. Results showed that the spatialproduction of aaptamine and isoaaptamine did not correlate with the quality of their surroundinghabitat, despite nitrite and nitrate levels significantly affected the bioactivity of crude extracts.
PERBANDINGAN BEBERAPA METODE ANALISIS HISTAMIN UNTUK PRODUK PERIKANAN Januar, Hedi Indra
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 4, No 2 (2009): August 2009
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (345.13 KB) | DOI: 10.15578/squalen.v4i2.150

Abstract

Studi ini  bertujuan  untuk  membandingkan  beberapa  metode dalam penentuan kadar amina  biogenik histamin. Diketahui, kadar histamin merupakan salah satu parameter yang penting  sebagai  standar  kualitas  produk  perikanan. Metode yang dibandingkan meliputi metode menggunakan spektrofluorometri, High Performance Liquid Chromatography (HPLC), Enzyme Linked Immunosorbent  Assay (ELISA), dan Capillary Electrophoresis/Capillary Zone Electrophoresis (CE/CZE). Hasil perbandingan menunjukkan bahwa baik dari sisi ketidakpastian yang mungkin ditimbulkan dari metodenya serta hasil uji kelayakan laboratorium di Eropa, metode HPLC derivatisasi post-kolom merupakan metode yang optimal saat ini untuk menentukan kadar histamin secara kuantitatif. Akan tetapi, untuk pertimbangan efisiensi waktu, maka studi ini mengusulkan bahwa penggabungan metode ELISA kualitatif dan HPLC kuantitatif sangat baik  dijadikan sebagai standar metode penentuan histamin di laboratorium pengujian produk perikanan.
Chemical Composition and Fatty Acid Profile of Some Indonesian Sea Cucumbers Fawzya, Yusro Nuri; Januar, Hedi Indra; Susilowati, Rini; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 1 (2015): May 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (614.077 KB) | DOI: 10.15578/squalen.v10i1.118

Abstract

Indonesia has a great sea cucumber resource which is useful as functional food. Even though much information about the biopotency of sea cucumbers have been published, however, studies on nutrition value of Indonesian sea cucumbers are still beneficial due to the great diversity and uniqueness of each species. The present work was  intended to obtain information about chemical composition and fatty acid profile of 4 (four) types of sea cucumbers which are frequently found in Halmahera water, North Maluku (Molucca),  one  of  sea  cucumbers  producing area  in Indonesia. The samples coded as  H-03, H-04, H-05 and H-10, were identified as Bohadschia  argus, Holothuria  fuscogilva, Thelenota  ananas, and Actinophyga lecanora. All sea cucumbers showed high protein content, more than 60% (drybase/db), except for T. ananas which had the lowest protein content (48.26% db). As for fats, T. ananas showed the highest amount (2.35%db) with dominated saturated fatty acids (SFA). On the other side, A. lecanora showed higher value of PUFA compared to SFA and MUFA.  Palmitic acid (C16:0) was the most abundant SFA in the most samples with the amount of 0.49–4.9 mg/g sample. Among PUFA, eicosapentaenoic acid/EPA (C20:5n3) was detected, and eicosatrienoic acid (C20:3n6) was relatively higher than the others. The ratio of w6/w3 fatty acid was 1.2–3.2 showing that total w6 fatty acid was higher than total w3 fatty acid. However, this value is safe enough to protect against chronic and degenerative diseases.
CYTOTOXIC SATURATED FATTY ACIDS FROM THE INDONESIAN SEA CUCUMBER Holothuria sp. Januar, Hedi Indra; Nursid, Muhammad; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 9, No 1 (2014): May 2014
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.322 KB) | DOI: 10.15578/squalen.v9i1.69

Abstract

Sea cucumbers are one of Indonesia’s marine organism with the potential to be developed as possible herbal medicines. In a preliminary study of cytotoxic activity of ethanol extracts of 14Indonesian sea cucumber species, the most active extract came from the Holothuria sp. Thecurrent research aimed to identify the major cytotoxic component in the sea cucumber Holothuriasp., that yielded the most active extract. The samples were collected from South Lampung beach.Isolation of the cytotoxic component was done employing liquid flash and preparative reversedphase (C 18) chromatography. Cytotoxic ity evalu atio n of fractio ns collected durin g thechromatography was conducted using the MCF-7 tumor cell line. Elucidation of the structure ofmost active isolate was done by NMR (Nuclear Magnetic Resonance) spectroscopy and GC-FID(Gas-Chromatography-Flame Ionisation Detector) analysis. The results of these analyses showedthe most active compound to be stearic acid; IC 50towards MCF-7 cells 10.32 ppm.