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Determination of Level of Food Additives in Labisia pumila (LP) Beverages Consumed in Kuantan, Malaysia

Agritech Vol 32, No 4 (2012)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

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Abstract

The content levels of several food additives (gallic acid, benzoic acid and caffeine) in commercial Labisia pumila (LP) beverage samples in Kuantan, Malaysia were determined by high performances liquid chromatography (HPLC). These analytical measurements were undertaken primarily to assess the compliance of content levels of the investigated food additives and their daily intake doses with permissible levels. The results obtained from this study indicated that the average levels of GA, caffeine and benzoic acid in the analyzed beverages were 37.62-229.35 ppm, 43.46 -168.00 ppm and 98.10-241.13 ppm, respectively. In addition, the concentrations of these food additives have been converted into daily intake doses based on beverage consumption. It was estimated that the mean daily intake of GA, caffeine and benzoic acid  by the adult population of Kuantan through the consumption of the analyzed beverages  were 0.39 mg/kg body weight/day for GA, 0.59 mg/kg body weight/day for caffeine (19.6 % ADI) and 0.43 mg/kg body weight/day for benzoic acid (8.6%ADI). None of the analyzed beverage sample was found to violate the current legal limits as stipulated in Malaysian food regulation.

IDENTIFIKASI DAN KUANTIFIKASI ASAM GALAT SEBAGAI SUMBER ANTIOKSIDAN PADA EKSTRAK DAUN KACIP FATIMAH (Labisia pumila var. alata) LARUT AIR

Jurnal Aplikasi Teknologi Pangan Vol 2, No 3 (2013): Agustus 2013
Publisher : Indonesian Food Technologists

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Abstract

Labisia pumila (Myrsinaceae) atau "Kacip Fatimah", secara tradisional digunakan dalam pengobatan tradisional Melayu sebagai tonik setelah melahirkan. Saat ini di Malaysia, Labisia pumila begitu populer sebagai makanan atau minuman fungsional. Perusahaan mengimpor bahan baku tanaman ini untuk memenuhi permintaan konsumen. Namun, informasi mengenai senyawa aktif dari tanaman ini sangat jarang dan langka. Tujuan penelitian ini adalah untuk mengidentifikasi, menghitung serta mengetahui pengaruh asal tumbuh terhadap kandungan asam galat Labisia pumila var alata (LP) asal Indonesia. Identifikasi dan kuantifikasi asam galat diukur dengan menggunakan metode kromatografi cair berkinerja tinggi (KCKT). Hasil menunjukan ekstrak LP larut air asal Indonesia memiliki karakteristik fisik: rendemen (10-11% b/b), total padatan (1.33˚brix) dan kelarutan dalam air 88% (b/b) (air dingin) dan 93% (b/b) (air panas). Ekstrak LP larut air yang berasal dari Gunung Tilu, Bogor memiliki kandungan asam galat (GA) tertinggi (1.86% b/b) dibandingan ekstrak LP larut air dari daerah lain. Kandungan asam galat (GA) pada tanaman LP dipengaruhi oleh faktor lokasi dan tempat asal tumbuh (p ≤ 0,01), tetapi tidak dengan karakteristik fisik (rendemen, total padatan dan kelarutan) (p ≥ 0.05). LPT memiliki sumber antioksidan alami, yang bermanfaat bagi kesehatan manusia dan dapat digunakan sebagai makanan fungsional untuk memenuhi kebutuhan diet.

POTENSI FRAKSI ETIL ASETAT DAUN TORBANGUN (Coleus amboinicus L.) DALAM MENINGKATKAN PRODUKSI SUSU, BOBOT BADAN INDUK, DAN ANAK TIKUS

Jurnal Gizi dan Pangan Vol 12, No 1 (2017)
Publisher : Food and Nutrition Society of Indonesia

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Abstract

The aims of the research were to investigate the potential medicine from ethyl acetate fraction of Coleus amboinicus L. for improving milk yield, body weight of rats and its effects on growth of the rat pups. Galactagogue activity was evaluated in terms of quantity of milk produced from the rats treated with ethyl acetate fraction of torbangun leaves (FEA), and commercial milk booster contained ‘katuk’ leaves extract (AF). Lactating rats (n=5) of Sprague dawley with six pups were fed with FEA and AF in the amount of 30 mg/kg and 50 mg/kg body weight, respectively. The feed were given orally every two days and starting from day 2 after giving birth until day 14. The performance of milk production was measured along the experimental period by weight-suckle-weight method. The rates of pups’ growth were measured as the weight gain along experimental period. The rats fed with ethyl acetate fraction of torbangun leaves produced higher milk than AF and the control groups, while FEA did not affected the body weight of rats in comparison to the control groups. Ethyl acetate fraction of torbangun leaves was identified to increase milk production by 17%, while AF by 22%. The mean of yields produced by the rats during lactation period for FEA, AF and control were 7.12±1.16, 7.43±1.04 and 6.07±0.85 g/pups/day, respectively. The FEA administration showed gradually increased of rat pups growth and body weight gain significantly in pups 2-14 days old compared to the control group. The present study reveals the ethyl acetate fraction has the potency as a remedy for improving milk yield without influencing the rats.

Minerals and heavy metals in Labisia pumila var. alata folia of Selected Geographic Origins

Jurnal Kartika Kimia Vol 2 No 1 (2019): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, Jenderal Achmad Yani University

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Abstract

Labisia pumila var. alata leaves (LP) with a long history of use folk remedy and endemic to the Malay Archipelago, is now supplied worldwide as ingredient of functional foods and beverages. Minerals and heavy metal concentrations in Labisia pumila var. alata folium (LP) of selected geographic origin based on microwave-assisted sample digestion and inductively coupled plasma mass spectrometry (ICP-MS) were determined.  Fifteen elements comprising minerals (Ba, Ca, Cr, Co, Cu, Fe, K, Mg, Na, Ni, Rb and Zn) and heavy metals (As, Cd and Pb) were analyzed with an inductively coupled plasma – mass spectrometer (ICP-MS). The highest nutrient concentration was measured in LP from Tilu Mountain (Cu, Na, K, Mg and Zn). The highest values of Ba, Ca, Co, Cr and Fe were detected in LP from Raub. Ni and Rb were highest in LP from Cibeundey Village. As was highest in LP from Raub (0.04 ± 0.00 mg/kg). The highest Pb contents were in LP from Tilu Mountain (2.90 ± 0.10 mg/kg) and LP from Halimunan-Salak Mountain (3.12 ± 0.03 mg/kg), all of which were well within the permissible limits as specified by the U.S. FDA for edible plant parts.  

Pengaruh Jenis Pelarut terhadap Kandungan Total Fenolik, Aktifitas Antioksidan dan Toksisitas Ekstrak Buah Ciplukan (Physalis angulata L)

Jurnal Riset Teknologi Industri Vol.13. No.1 JUNI 2019
Publisher : Balai Riset dan Standardisasi Industri Samarinda

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Abstract

In Indonesia, most people have used herbal products to maintain their health. Ciplukan plant is one of the local wisdom that is believed to be able to retain and treat various diseases, such as Diabetes mellitus.its has potential as a functional food. Ciplukan contains bioactive components, where the extraction process and the use of solvent types play an important role. This study aims to determine the effect of solvent on total phenolics, antioxidant activities and toxicity of ciplukan (Physalis angulata L.)fruit. The experimental design used a completely randomized design (CRD) with three different solvents, namely: 70% ethanol (a1), ethyl acetate (a2), and n-hexane (a3), six replications. Total phenolic content, total flavonoids, antioxidant activity (DPPH method), and toxicity (Method of Brine Shrimp Lethality Test) of mature ciplukan fruit extract were analyzed. The results showed that the ethanol extract of ciplukan fruit (a1) had the highest total phenolic content (140.50 mg GAE / g extract) and total flavonoids (100.46 mg QE / g) followed by a2> a3. Antioxidant activity showed that a1 had the lowest IC50 (321.02 μg / mL), and had the highest antioxidant activity, compared to a2 and a3. Based on toxicity test, LC50 value of ethanolic extract of ciplukan (a1) (886,1 μg / mL) as cytotoxic. This study can conclude that the type of solvent affects the total phenolics, antioxidant activitiesand toxicity of ciplukan fruit extract.ABSTRAKDi Indonesia, produk herbal telah digunakan sebagian besar masyarakat untuk menjaga kesehatan. Tanaman ciplukan merupakan salah satu kearifan lokal yang dipercaya dapat menjaga dan mengobati berbagai penyakit, sepertiDiabetes mellitus dan memiliki potensi sebagai pangan fungsional.Ciplukan mengandung komponen bioaktif, dimana proses ekstraksi dan penggunaan jenis pelarut memainkan peranan penting.Tujuan dari penelitian ini adalah untuk mempelajari pengaruh jenis pelarut terhadap kandungan total fenolik, aktifitas antioksidan dan toksisitas ekstrakbuahciplukan (Physalis angulata L.). Desain eksperimental menggunakan rancangan acak lengkap (RAL) dengan menggunakan3 pelarut yang berbeda, yaitu: etanol 70% (a1), etil asetat (a2), dan n-heksana (a3), dengan6 replikasi. Kandungan total fenolik, total flavonoid, aktifitas antioksidan (metode DPPH), dan toksisitas (Metode Brine Shrimp Lethality Test) ekstrak buah ciplukan diteliti. Hasil penelitian menunjukkan bahwa ekstrak etanol buah ciplukan (a1) memiliki kandungan total fenolik tertinggi (140,50 mg GAE/g ekstrak) dan total flavonoid(100,46 mg QE/g) diikuti olehekstrak etil asetat buah ciplukan(a2) >ekstrak n-heksana buah ciplukan (a3). Aktifitas antioksidan menunjukkan a1 memiliki IC50 terendah (321,02 μg/mL), memiliki aktifitas antioksidan tertinggi, dibandingkan a2 dan a3. Uji toksisitas menunjukkan bahwa nilai LC50 ekstrak etanol buah ciplukan (a1) (886,1μg/mL) bersifat sitotoksik dibandingkan a2 dan a3.Penelitian ini menyimpulkan bahwa jenis pelarut yang digunakan dalam mengekstrak buah ciplukan dapat mempengaruhi kandungantotal fenolik, aktifitas antioksidandan toksisitas.Kata kunci:aktifitas antioksidan, ciplukan, Physalis angulata, fitokimia, toksisitas.