Enade Perdana Istyastono
Pharmaceutical Technology Lab., Faculty of Pharmacy, Sanata Dharma University, Yogyakarta, Indonesia 55284.

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ANALISIS REFLEKSI PADA PEMBELAJARAN: REVIEW REASEARCH Yuliyanto, Eko; Hidayah, Fitria Fatichatul; Istyastono, Enade Perdana; Wijoyo, Yosef
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Profesionalisme guru penting dalam menunjang proses pembelajaran di kelas. Indonesia adalah negara dengan kualitas pendidikan yang belum baik. Banyak faktor yang menyebabkan hal tersebut. Salah satu faktor adalah Sumber Daya Manusia, Guru. Seorang pendidik di sekolah dituntut memiliki 3 kompetensi wajib  yaitu  pedagodik,  social  dan professional. Pada  kompetensi  pedagogic menjadi kebutuhan mutlak bagi seorang guru. Untuk menjadi guru yang mampu mengajar di kelas dengan baik membutuhkan pengalaman yang cukup banyak dan waktu yang lama. Namun, jika harus demikian makan kebutuhan guru di lapangan  akan  sulit  terpenuhi.  Ada  salah  satu  cara  yang  mampu  untuk membantu meningkatkan kapasitas guru dalam mengajar. Alternatif tersebut berupa kemampuan refleksi diri seorang guru. Kebiasaan refleksi yang saat ini ada  pada  beberapa  guru  di  Indonesia  masih  sedikit,  salah  satunya  ?Lesson study?, namun jumlah komunitas ini tidak begitu banyak, Oleh karena itu untuk mengetahui berbagai bentuk relfeksi guru yang ada di Indonesia diperlukan suatau  penelitian  untuk  memetakan  kondisi  tersebut.  Penelitian  ini  adalah Review Reasearch dengan objek berbagai artikel hasil penelitian di Indonesia yang meneliti tentang ?refleksi? guru dalam pembelajaran. Berdasarkan hasil review   artikel   penelitian   diperoleh   banwa   bentuk-bentuk   refleksi   yang dilakukan  guru  di  Indonesia  berupa:  jurnal  reflksi,  refleksi  wawancara, konfrensi observasi temanejawat, diskusi group, Video, Blok, dan portofolio elektronik. Kata Kunci : Refleksi, Pembelajaran
KEUNGGULAN PEMBELAJARAN REFLECTIVE PEDAGOGY PARADIGM (RPP) UNTUK PERBAIKAN PROSES PEMBELAJARAN DI PERGURUAN TINGGI Yuliyanto, Eko; Hidayah, Fitria Fatichatul; Istyastono, Enade Perdana; Wijoyo, Yosef; Hartayu, Titien Siwi
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Pendidikan, Sains dan Teknologi
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Pendidikan di perguraun tinggi merupakan pembelajaran sangat berbeda dengan pembelajaran tingkat SMA. Namun, hal itu juga menjadi perhatiankhusus supaya proses transfer ilmu pengetahuan dari dosen ke mahasiswaberlangsung dengan baik. Tujuan penelitian ini mengetahu keunggulan RPPdalam penerapan pembelajaran di tingkat perguruan tinggi. Metode penelitianini menggunakan kajian referensi, observasi, dan wawancara. Hasil penelitianmenunjukkan bahwa pembelajaran di perguruan tinggi memerlukan perhatiankhusus terutama pada metode-metode yang digunakan. Adanya keunggulanRPP diharapkan dapat membuka pengetahuan para dosen untuk mampumelakukan pembelajaran RPP: membangu konteks, penglaman,refleksi, aksidan evaluasi, sehingga kebermaknaan belajar para mahasiswa akan tertatadengan baik dan sesuai dengan outcome yang diharapkan oleh program studi.    Keywords: Reflective Pedagogy Paradigm, Keunggulan, Pembelajaran,Perguruan Tinggi
DOES REFLECTIVE PEDAGOGY MAKE ENVIROMENTAL LACTURER BE BETTER? Yuliyanto, Eko; Hidayah, Fitria Fatichatul; Istyastono, Enade Perdana; Wijoyo, Yosef
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: PROCEEDING 1ST INSELIDEA INTERNATIONAL SEMINAR ON EDUCATION AND DEVELOPMENT OF ASIA (INseIDEA)
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Environmental education is an aspect of education for students who are important to support life in the future. In the learning process there are several factors that influence success in learning. One of them is the ability factor of teaching from a teacher include material, method and learning media. However, there are important factors that need to be considered, namely the ability of teachers to reflect themselves during the learning process. Therefore it is necessary for the research to know the reflection of teachers in the learning process. This study aims to determine the effect of teacher reflection on student learning outcomes. The study involved as many as 15 students of English education. Data obtained in the form of student reflection data, questionnaire value of proenvironment attitudes. The data obtained were analyzed using Paired Samples T-Test. The results showed that the process of reflection that has not been optimal can?t improve the quality of learning.Keywords: pro-environment, reflection of paradigm pedagogy, lecturer, learning
VIRTUAL SCREENING CAMPAIGNS ON ISOFLAVONES TO DISCOVER POTENT CYCLOOXYGENASE-2 INHIBITORS Istyastono, Enade Perdana
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 14, No 2 (2017)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (505.928 KB) | DOI: 10.24071/jpsc.142698

Abstract

By employing recently published structure-based virtual screening (SBVS) to identify potent cyclooxygenase-2 (COX-2) inhibitors, all isoflavones collected by ZINC15 database were virtually screened. There were 3371 isoflavones in ZINC15 database and 1356 compounds out of them met the Lipinski’s rule of 5. Notably, only 3 isoflavones out of those 1356 compounds were identified as potent COX-2 inhibitors.
Activity of propyl p-benzoyloxybenzoate as mu-class glutathione s-transferase inhibitor Harnita, Agnes Nora Iska; Istyastono, Enade Perdana; Martono, Sudibyo
INDONESIAN JOURNAL OF PHARMACY Vol 17 No 2, 2006
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.279 KB) | DOI: 10.14499/indonesianjpharm0iss0pp76-80

Abstract

Non-steroidal anti-inflammatory drugs (NSAIDs) are the most prescribed medicines. Anti-inflammatory activity was reported to have relationship with the inhibition of prostaglandin synthesis, one of the inflammation mediators. The inhibition mechanism might be through the cyclooxygenase (COX) inhibition, oxygen radical scavenging, and mu-class glutathione S-transferase (GST) inhibition. Aspirin has been used as a NSAID since a hundred years ago and was reported as cyclooxigenase-1 (COX-1) selective inhibitor. The selectivity leds to gastrointestinal ulceration. Propyl p-benzoyloxybenzoate was a new compound which was predicted to have anti-inflammatory activity and would be developed to be an NSAID with minimum side effect.mu-class GST inhibition was examined using formation reaction model of GS-CNB conjugate through conjugation of 1,2-dichloro-4-nitrobenzene (DCNB) and glutathione (GSH) with GST (prepared from rat’s liver) as a catalyst. GSTs were isolated from the rat liver cytosolic fraction by centrifugation according to Lundgren. Protein concentration of the cytosol was determined spectrophotometrically by using bovine serum albumin as a standard. The GST activity was determined using conjugation reaction rate between DCNB and GSH, followed by determination of IC50 of propyl p-benzoyloxybenzoate. The result showed that propyl p-benzoyloxybenzoate has activity as  mu-class GST inhibitor with IC50 = 111.77 μM as the result from extrapolation.Key words: Anti-inflammatory, propyl p-benzoyloxybenzoate, inhibitor, mu-class glutathione S-transferase (GST).
Quantitative structure-activity relationship of curcumin and its derivatives as μ-class of GST inhibitors Istyastono, Enade Perdana; Martono, Sudibyo
INDONESIAN JOURNAL OF PHARMACY Vol 16 No 4, 2005
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (224.779 KB) | DOI: 10.14499/indonesianjpharm0iss0pp204-213

Abstract

The Quantitative Structure-Activity Relationship (QSAR) study has been performed on curcumin and its derivatives as μ-class glutathione Stransferase inhibitors using atomic net charges as the predictors. The charges were resulted by semiempirical AM1 quantum-chemical calculations using the computational chemistry approach. The inhibition activity was expressed as the concentration that gave 50% inhibition of μ-class GST activity (IC50). The selection of the best QSAR equation models was determined by multiple linear regression analysis. Curcumin and its derivatives were reported as selective μ-class GST inhibitors. The enzyme plays an important role in the process of inflammation and the effectivity of anti lung-cancer compounds. μ-Class GST inhibitors could enhance the effectivity of anti lung-cancer compounds.The result showed that the best QSAR equation model of curcumin and its derivatives as μ-class GST inhibitors was described by.: log (1/IC50) = (-454.686) + (314.235)qC2 + (1593.499)qC3 + (452.563)qC4 + (1057.194)qC5 + (-1.756)qBenzene’  The equation was significant at 95% level with statistical parameters : n = 10, m = 5, F/FTabel = 3.335, R2 = 0.963, SE = 0.150, and PRESS = 0.559.Key words : QSAR analysis, curcumin, μ-class glutathione S-transferase, atomic net charge.
CONSTRUCTION AND RETROSPECTIVE VALIDATION OF STRUCTURE-BASED VIRTUAL SCREENING PROTOCOLS TO IDENTIFY POTENT LIGANDS FOR HUMAN ADRENERGIC BETA-2 RECEPTOR Istyastono, Enade Perdana; Setyaningsih, Dewi
INDONESIAN JOURNAL OF PHARMACY Vol 26 No 1, 2015
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (717.965 KB) | DOI: 10.14499/indonesianjpharm26iss1pp20

Abstract

Adrenergic Beta-2 Receptor (ADRB2) is a member of G-protein coupled receptors family, which has served as targets for more than 30% of top-selling drugs in the market. Recently, an enhanced dataset of ligands and decoys for ADRB2 has publicly available. However, the original retrospective structure-based virtual screening campaign accompanying the dataset showed relatively poor quality with enrichment factor of true positives at 1% false positives (EF1%)value of 3.9. In this article, the construction and retrospective validation of a structure-based virtual screening protocol by employing PLANTS1.2 as the molecular docking software and PyPLIF as an alternative post docking scoring functions are presented. The results show that the developed protocols have better quality compared the original structure-based virtual screening with EF1% values of 24.24 and 8.22 by using ChemPLP from PLANTS1.2 and by using Tc-PLIF from PyPLIF, respectively. Further investigation by performing systematic filtering resulted in the identification of D113, S203, and N293 as molecular determinants in ADRB2-ligand binding.Key words: Structure-based virtual screening, molecular docking, adrenergic beta-2 receptor, protein-ligand interaction fingerprinting, molecular determinants
EFFECT OF ASPIRIN ON RAT LIVER CLASS- GLUTATHIONE S-TRANSFERASE ACTIVITY Istyastono, Enade Perdana; Martono, Sudibyo; A.M., Supardjan
INDONESIAN JOURNAL OF PHARMACY Vol 13 No 2, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm0iss0pp71-76

Abstract

Inflammation is the response of living tissues caused by injury. It involves a complex battery of enzyme activation, mediator release, extravasation of fluid, cell migration, tissue breakdown and repair. Inflammation mediator is synthesized in the body by several steps and catalyzed by several enzymes, such as cyclooxygenase and class- glutathione S-transferases (GSTs) in the cyclooxygenase arachidonic acid cascade. Aspirin, which has been reported as an inhibitor to inflammation mediator synthesis in the cyclooxygenase arachidonic acid cascade, is a non-steroid anti-inflammatory agent. The aim of this research is to study the effect of aspirin on rat liver class- GST activity in vitro. GSTs were isolated from the rat liver cytosolic fraction by centrifugation according to Lundgren. Protein concentration in the cytosol was determined spectrophotometrically using bovine serum albumin as a standard. The GST activity was determined using conjugation reaction rate between glutathione (GSH) and 1,2-dichloro-4-nitrobenzene (DCNB), followed by determining IC50 of aspirin. Then, a study was done to determine the ability of aspirin in inhibition of the rat liver class- GST activity in vitro. It can be concluded that aspirin has no inhibitory effect on rat liver class- GST activity.Key word: Aspirin, anti-inflammatory, glutathione S-transferase.
Synthesis of a potential angiogenesis inhibitor compound: 2-benzylidene cyclohexane-1,3-dione Istyastono, Enade Perdana; Yuniarti, Nunung; ., Jumina
INDONESIAN JOURNAL OF PHARMACY Vol 20 No 1, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (567.187 KB) | DOI: 10.14499/indonesianjpharm0iss0pp1-8

Abstract

Synthesis of an aromatic cyclic enadione, 2-benzylidene-cyclohexane-1,3-dione (a potential angiogenesis inhibitor), has been done using benzaldehide (50 mmole) and cyclohexane-1,3-dione (50 mmole) as starting materials and NaOH 1.0 N as a catalyst. Odorless and white needle-shape powder has been achieved. The powder was 252.7 mg (2.53 %) and the purity of the powder was 88.81 %. Its melting point was 214.8 oC. The purity of the product was examined by gas chromatography, while the structure elucidation was done using IR spectrometer, 1H-NMR spectrometer, and GC-MS.IR spectrometer showed that the compound had a -C=O bond conjugated to an alkene or a phenyl moiety, methylen groups, and alkene’s -C–H bonds. 1H-NMR spectrometer showed that the compound had benzene moiety and methylen moieties, which were in α and β position to a carbonyl group. GC-MS showed that the molecular weight of the compound was 200 atomic mass unit.Key words: Angiogenesis inhibitor, synthesis, 2-benzylidene-cyclohexane-1,3-dione.
The Keto-Enol Tautomerism of Curcumin and Some 4-substituted Curcumin Derivatives : A Theoretical Study Based on Computational Chemistry Approach Istyastono, Enade Perdana; Margono, Supardjan A.; Pranowo, Harno Dwi
INDONESIAN JOURNAL OF PHARMACY Vol 14 No 3, 2003
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.263 KB) | DOI: 10.14499/indonesianjpharm0iss0pp107-113

Abstract

Heat formation (DHf) for the enol and diketo tautomers of curcumin and some 4-substituted curcumin derivatives has been studied using semiempirical AM1 quantum-chemical calculations. The calculations on curcumin, 4-methylcurcumin, 4-ethylcurcumin, 4-npropylcurcumin, 4-isopropyl-curcumin, 4-n-butylcurcumin, and 4-benzylcurcumin showed that the more steric hindrance resulted from the subtituents, the more stable they were in the form of diketo tautomers than enol tautomers. Whereas, calculations on 4-phenylcurcumin, 4-(o-methoxyphenyl)-curcumin, and 4-(p-methoxyphenyl) curcumin showed that though steric hindrance influenced the keto-enol tautomerism, they were found more stable in the form of enol tautomers. This phenomenon was estimated to be related with conjugation effect of the aromatic groups with the enol moiety of the main bone structure.Keywords : 4-Substituted curcumin, the keto-enol tautomerism, AM1, semiempirical calculations.