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Selective Media for In Vitro Activity Evaluation of Bacterial Biocontrol Against Pathogenic Vibrio

HAYATI Journal of Biosciences Vol 18, No 3 (2011): September 2011
Publisher : Bogor Agricultural University, Indonesia

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Abstract

In vitro activity test is a critical evaluation to screen the potential biocontrol agent. We developed a selective medium for quantitative in vitro activity evaluation of bacterial biocontrol agents against pathogenic Vibrio in aquaculture. Sensitivity test of bacterial biocontrol and Vibrio spp. to nine antibiotics showed that oxytetracycline inhibited the growth of Vibrio spp., but did not inhibit the growth of the bacterial biocontrol. This selective inhibition activity of oxytetracycline suggested this antibiotic might be supplemented to establish a selective medium. The MIC of oxytetracycline against V. harveyi, V. parahaemolyticus, and V. algosus were 120, 250, and 120 mg/ml, respectively. These concentration did not inhibit the growth of bacterial biocontrols. Therefore, oxytetracycline was supplemented at 250 µg/ml in Zobell agar medium. This medium was used as a selective medium to enumerate the density of bacterial biocontrol. In vitro activity test of bacterial biocontrol (RLP1) against V. parahaemolyticus showed that strain RLP1 at density of 104 and 106 cells/ml was able to kill V. parahaemolyticus during 6 h incubation. At lower density, 102 cells/ml, this bacterial biocontrol agent was able to kill the pathogenic Vibrio during 12 h incubation. This study discovered a selective medium for the bacterial biocontrol and Vibrio spp. and provided the results of its application in the evaluation of in vitro activity of a bacterial biocontrol agent against V. parahaemolyticus. The results also revealed that strain RLP1 is a potential bacterial biocontrol against vibriosis in marine aquaculture.

Patogenisitas Vibrio Fluvialis 24SK terhadap Kerapu Tikus (Cromileptes altivelis)

Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

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Abstract

This research was aimed to investigate the pathogenicity of Vibrio fluvialis 24SK in humpback grouper (Cromileptes altivelis) based on its Lethal Dosage 50 (LD50). V. fluvialis 24SK was isolated from ren of humpback grouper cultured in floating net cage at Brackishwater Aquaculture Development Center (BADC) Situbondo, with vibriosis signs. The bacterium was cultured in Tryptone Soy Broth (TSB) medium dissolved in trisalt solution (KCl, 0.75 g/l; MgSO4.7H2O; 14.2 g/l; NaCl, 18.4 g/l), incubated at 300C for 24 h. Infection was carried out by interperitoneal injection to humpback grouper (8-9 cm of total length) at 102, 104, 106, and 108 cfu/fish. Control fishes were injected with 0.2 ml trisalt solution. Disease sign and mortality of fishes were observed every eight hour for 40 days. LD50 was calculated based on Dragstedt Behrens method (Hubert, 1980). Result indicated that infection of the bacteria at 106 and 108 cfu/fish caused sub-acute disease signs, such as haemorhagic on operculum, base of fins (pinnae pectorales, pinnae abdominales, pinna analis), and also head and abdomen, while infection at 102 and 104 cfu/fish caused chronic disease signs, such as haemorhagic on fins base which was followed by necrotic on fins and skin tissue in prolonged time. Histopathologically, infection of the bacteria caused atrophy on the gills, infiltrations of lymphocyte, heterofel and plasma cell on the gills and fins base, vacuolar degeneration on the liver, and also present the bacteria colony on the fins base and intestine tissues. V. fluvialis 24SK has LD50 at (1,1±0,5)x107 cfu/fish.

ISOLASI, KARAKTERISASI, DAN PATOGENITAS BAKTERI PENYEBAB PENYAKIT PADA GURAMI (Osphronemus goramy) DI KABUPATEN BANTUL

Jurnal Perikanan Universitas Gadjah Mada Vol 15, No 2 (2013)
Publisher : Universitas Gadjah Mada

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Abstract

Gouramy (Osphronemusgoramy) is an important fish comodity and preferred to be consumed by people. One of the limiting factor in freshwater aquaculture is bacterial infections. The objective of this research was to identify the species of pathogenic bacteria on gourami from Bantul Regency and to determine their pathogenicity. Eighteen diseased gouramy were collected from seven different fi sh farms. Bacteria were isolated from kidney and purifyon TSA medium. Characterization was conducted on the morphology of bacterial colonies and cells, biochemical tests. Koch Postulate Test was done to confirm pathogenic properties of the isolated bacteria. Pathogenicity test was performed by intraperitoneal injection of a serial doses of bacteria into gouramy and the Lethal Dosage 50 (LD50) was calculated. Twenty isolates of Aeromonas hydrophila, 5 isolates of Aeromonas salmonicida and one isolate of Vibrio cholerae,Vibrio anguillarum and Pseudomonas fluorescens respectively were isolated from and confi rmed as pathogen ingouramy. Pathogenic diversity was found among isolated bacteria, which a group of virulent bacteria as Aeromonas hydrophila GBNB, GGRB, and GCMA3 with LD50 values of 6.28 x 105, 2.09 x 105 and 5.37 x 105 CFU /fish; and a group of non virulent bacteria as A. hydrophila GKRA isolate and Vibrio cholera GPDA isolat with LD50 value of 7.14 x 107 and 3.45 x 107 CFU/fish respectively.

ISOLASI, IDENTIFIKASI DAN KARAKTERISASI Vibrio spp. PATOGEN PENYEBAB VIBRIOSIS PADA KERAPU DI BALAI BUDIDAYA AIR PAYAU SITUBONDO

Jurnal Perikanan Universitas Gadjah Mada Vol 7, No 1 (2005)
Publisher : Universitas Gadjah Mada

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Abstract

This research was conducted to isolate, identify, and characterize pathogenic Vibrio spp., causative agents of vibriosis in grouper at Brackishwater Aquaculture Development Center (BADC), Situbondo. Twenty-nine isolates were isolated from wound, gills, eyes, liver, and ren of grouper with vibriosis on Thiosulphate Citrate Bile Sucrose Agar (TCBSA) medium. Koch Postulate Test was conducted to determine pathogenic Vibrio spp., by intraperitoneal injection to humpback grouper (Cromileptes altivelis) (9-10 cm of total length) at 106 cells/fish in triplicates. Results indicated that the pathogenic Vibrio spp., causative agents of vibriosis in grouper at BADC, Situbondo were identified to be V. alginolyticus, V. anguillarum, V. metchnikovii, V. vulnificus, V. fluvialis, V. furnisii, and V. parahaemolyticus. Infection of the pathogenic Vibrio spp. caused acute mortality within 17-46 hours with specific disease signs like haemorhagic on fins (pinnae pectorales, pinnae abdominales, pinna analis) and also on the body. These results suggested that vibriosis should be monitored and controlled properly and quickly.

Growth, Total Production and Feed Efficiency of Catfish (Clarias sp.) Orally Administered with Shrimp Waste Hydrolyzate

Aquacultura Indonesiana Vol 19, No 1 (2018)
Publisher : Indonesian Aquaculture Society (MAI)

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Abstract

Shrimp Waste Hydrolyzate (SWH) /Shrimp Soluble Extract (SSE) is a product produced from the enzymatic process of shrimp waste. SWH contains essential and non-essential amino acids which useful for fish. This study aimed to determine effect of SWH on the growth and total production of catfish. The parameters observed were average growth rate, specific growth rate (SGR), food conversion ratio (FCR), protein efficiency ratio (PER), total production and length weight relationship from beginning until the end of rearing period. Growth performance was observed by calculating average growth rate and SGR of catfish. The results of this study showed that oral administration of SWH significantly increased SGR of catfish length at 4th sampling period with value 1.54% body length/day. Administration of SWH did not affect on the AGR, FCR, PER and total production. The growth pattern of catfish is negative allometric.